As an excellent local crop variety of Huanggang,"Huangzhou radish" has been widely promoted and its market influence is increasing day by day.Recently,the purity of "Huangzhou radish" has declined ...As an excellent local crop variety of Huanggang,"Huangzhou radish" has been widely promoted and its market influence is increasing day by day.Recently,the purity of "Huangzhou radish" has declined deeply,so it is urgent to improve and strengthen the purity and viability of "Huangzhou radish" including bad phenomena such as degeneration of varieties after self-pollination.At present,there was no SSR molecular marker can be used for genetic diversity identification of "Huangzhou radish".SSR molecular marker can provide a favorable tool to study "Huangzhou radish".In this study,"Xiakang","Hongbao"and "Huangzhou radish" were used as experimental materials,100 SSR primers were screened,and 25 pairs of SSR primers were selected.Finally,two pairs of primers were selected for identification of the purity of "Huangzhou radish" by non-denatured polyacrylamide gel electrophoresis (PAGE).The results showed that R-102 and R-112 primer pairs could amplify the special bands for "Huangzhou radish" from above three kinds of radishes.These two pairs of primers could be developed as SSR markers to identify the specificity and purity of "Huangzhou radish".Furthermore,this study provides convenient conditions for promoting the economic and social value of "Huangzhou radish".展开更多
In the present context of increasing social demands for natural science education,increasing people s awareness of environmental biodiversity protection,and ecological civilization lifting to the state strategy,it is ...In the present context of increasing social demands for natural science education,increasing people s awareness of environmental biodiversity protection,and ecological civilization lifting to the state strategy,it is just the time to explore a new botany field practice model.The attempt of a new task-driven model for botany field practice will greatly enhance students thinking about plants and nature,plants and environment,and plant and ecological civilization,and will inevitably enhance students initiative awareness and practical ability to protect and rationally utilize plant resources.展开更多
基金Supported by the Hubei Special Project for Development of Science and Technology in Local by Central Guidance(2018ZYYD019)
文摘As an excellent local crop variety of Huanggang,"Huangzhou radish" has been widely promoted and its market influence is increasing day by day.Recently,the purity of "Huangzhou radish" has declined deeply,so it is urgent to improve and strengthen the purity and viability of "Huangzhou radish" including bad phenomena such as degeneration of varieties after self-pollination.At present,there was no SSR molecular marker can be used for genetic diversity identification of "Huangzhou radish".SSR molecular marker can provide a favorable tool to study "Huangzhou radish".In this study,"Xiakang","Hongbao"and "Huangzhou radish" were used as experimental materials,100 SSR primers were screened,and 25 pairs of SSR primers were selected.Finally,two pairs of primers were selected for identification of the purity of "Huangzhou radish" by non-denatured polyacrylamide gel electrophoresis (PAGE).The results showed that R-102 and R-112 primer pairs could amplify the special bands for "Huangzhou radish" from above three kinds of radishes.These two pairs of primers could be developed as SSR markers to identify the specificity and purity of "Huangzhou radish".Furthermore,this study provides convenient conditions for promoting the economic and social value of "Huangzhou radish".
基金Supported by Special Fund for Reform of Teaching Model of Huanggang Normal University(2016CK06,2018CE42)
文摘In the present context of increasing social demands for natural science education,increasing people s awareness of environmental biodiversity protection,and ecological civilization lifting to the state strategy,it is just the time to explore a new botany field practice model.The attempt of a new task-driven model for botany field practice will greatly enhance students thinking about plants and nature,plants and environment,and plant and ecological civilization,and will inevitably enhance students initiative awareness and practical ability to protect and rationally utilize plant resources.
