Objective:To explore the correlation between epicardial fat thickness(EFT),aortic velocity propagation(AVP),and abdominal aortic intima-media thickness(AA-IMT)in patients with subclinical hypothyroidism(SH).Additional...Objective:To explore the correlation between epicardial fat thickness(EFT),aortic velocity propagation(AVP),and abdominal aortic intima-media thickness(AA-IMT)in patients with subclinical hypothyroidism(SH).Additionally,to compare these indicators between SH patients and healthy individuals,providing a new theoretical basis for the clinical prevention and treatment of cardiovascular diseases.Method:Clinical data from 50 SH patients(23 males and 27 females)and 50 healthy outpatient examinees(22 males and 28 females)were analyzed.The participants were selected from January 2022 to December 2023 at Loudi Central Hospital.EFT,AVP,and AA-IMT were measured,and their correlations were analyzed.Results:SH patients had significantly higher EFT and AA-IMT levels than the control group,while their AVP was significantly lower,with these differences being statistically significant(P<0.05).Correlation analysis revealed a significant negative correlation between EFT and AVP(P<0.001),a significant positive correlation between EFT and AAO-IMT(P<0.001),and a significant negative correlation between AVP and AAO-IMT(P<0.001).Multivariate binary logistic regression analysis identified increased EFT,decreased AVP,and increased AAO-IMT as independent risk factors for SH patients.Conclusion:In SH patients,EFT and AAO-IMT are elevated,whereas AVP is reduced.EFT and AVP are significantly correlated with AAO-IMT.EFT and AAO-IMT can serve as reliable indicators for evaluating subclinical atherosclerosis in SH patients,providing a diagnostic basis for clinical practice.展开更多
The detection of manganese(Mn)in steel by laser-induced breakdown spectroscopy(LIBS)provides essential information for steelmaking.However,self-absorption greatly disrupts the LIBS spectral lines of Mn with high conte...The detection of manganese(Mn)in steel by laser-induced breakdown spectroscopy(LIBS)provides essential information for steelmaking.However,self-absorption greatly disrupts the LIBS spectral lines of Mn with high content.In this study,to minimize self-absorption for Mn spectral lines in LIBS,laser-induced fluorescence(LIF)was applied.Compared with conventional LIBS,the self-absorption factors(α)of Mn I 403.08,403.31,and 403.45 nm lines were reduced by 90%,88%,and 88%,respectively;the root mean square errors of crossvalidation were decreased by 88%,85%,and 87%,respectively;the average relative errors were reduced by 93%,90%,and 91%,respectively;and average relative standard deviations were decreased by 29%,32%,and 33%,respectively.The LIBS-LIF was shown to successfully minimize the self-absorption effect and spectral intensity fluctuation and improve detection accuracy.展开更多
Co–Mo catalysts applied on the hydrodesulfurization（HDS） for FCC gasoline were prepared with Zn–Al layered double hydroxides（LDHs） to improve their performances,and the effects of pore structures and acidity on ...Co–Mo catalysts applied on the hydrodesulfurization（HDS） for FCC gasoline were prepared with Zn–Al layered double hydroxides（LDHs） to improve their performances,and the effects of pore structures and acidity on HDS performances were studied in detail. A series of Zn–Al/LDHs samples with different pore structures and acidities are synthesized on the bases of co-precipitation of OH-,CO2-,Al3＋,and Zn2＋. The neutralization p H is a main factor to affect the pore structures and acidity of Zn–Al/LDHs,and a series of Zn–Al/LDHs with different pore structures and acidities are obtained. Based on the representative samples with different specific surface areas（SBET） and acidities,three Co Mo/LDHs catalysts were prepared,and their HDS performances were compared with traditional Co Mo/Al2O3 catalysts. The results indicated that catalysts prepared with high SBETpossessed high HDS activity,and Br？nsted acid sites could reduce the thiol content in the product to some extent. All the three catalysts prepared with LDHs displayed little lower HDS activity but higher selectivity than Co Mo/Al2O3,and could restrain the reactions of re-combination between olefin and H2 S which could be due to the existence of Br？nsted acid sites.展开更多
Polycrystalline La-doped CaMnO3 ceramics have been prepared by a solid-state sintering method. Analysis of microstructure and phase composition indicates that the addition of La can prohibit the further growth of grai...Polycrystalline La-doped CaMnO3 ceramics have been prepared by a solid-state sintering method. Analysis of microstructure and phase composition indicates that the addition of La can prohibit the further growth of grain, and no impurity phase appears. The results revealed that the La doping can lead to a large change of the activation energy （from 0.2：2 to 0.02 eV）, and thus result in a marked increase in electric conductivity of 2-4 orders of magnitude. The power factor can reach about 1.5×10-4 W.m-1.K-2 in a wide temperature range, which potentially make them attractive for n-type high-temperature thermoelectric materials.展开更多
Membrane distillation(MD)is a promising membrane separation technique used to treat industrial wastewater.When coupled with cheap heat sources,MD has significant economic advantages.Therefore,MD can be combined with s...Membrane distillation(MD)is a promising membrane separation technique used to treat industrial wastewater.When coupled with cheap heat sources,MD has significant economic advantages.Therefore,MD can be combined with solar energy to realize the large-scale and low-cost treatment of highly mineralized mine water in the western coalproducing region of China.In this study,highly mineralized mine water from the Ningdong area of China was subjected to vacuum MD(VMD)using polyvinylidene fluoride hollow-fiber membranes.The optimal operation parameters of VMD were determined by response surface optimization.Subsequently,the feasibility of VMD for treating highly mineralized mine water was explored.The fouling behavior observed during VMD was further investigated by scanning electron microscopy with energy-dispersive X-ray spectroscopy(SEM-EDS).Under the optimal parameters(pressure=-0.08 MPa,temperature=70℃,and feed flow rate=1.5 L/min),the maximum membrane flux was 8.85 kg/(m^(2) h),and the desalination rate was 99.7%.Membrane fouling could be divided into three stages:membrane wetting,crystallization,and fouling layer formation.Physical cleaning restored the flux and salt rejection rate to 94%and 97%of the initial values,respectively;however,the cleaning interval and cleaning efficiency decreased as the VMD run time increased.SEM-EDS analysis revealed that the reduction in flux was caused by the precipitation of CaCO_(3).The findings also demonstrated that the membrane wetting could be attributed to the formation of NaCl on the cross section and outer surface of the membrane.Overall,the results confirm the feasibility of MD for treating mine water and provide meaningful guidance for the industrial application of MD.展开更多
Supramolecular peptide nanofiber hydrogels are emerging biomaterials for tissue engineering,but it is difficult to fabricate multi-functional systems by simply mixing several short-motif-modified supramolecular peptid...Supramolecular peptide nanofiber hydrogels are emerging biomaterials for tissue engineering,but it is difficult to fabricate multi-functional systems by simply mixing several short-motif-modified supramolecular peptides because relatively abundant motifs generally hinder nanofiber cross-linking or the formation of long nanofiber.Coupling bioactive factors to the assembling backbone is an ideal strategy to design multi-functional supramolecular peptides in spite of challenging synthesis and purification.Herein,a multi-functional supramolecular peptide,P1R16,is developed by coupling a bioactive factor,parathyroid hormone related peptide 1(PTHrP-1),to the basic supramolecular peptide RADA16-I via solid-phase synthesis.It is found that P1R16 self-assembles into long nanofibers and co-assembles with RADA16-I to form nanofiber hydrogels,thus coupling PTHrP-1 to hydrogel matrix.P1R16 nanofiber retains osteoinductive activity in a dose-dependent manner,and P1R16/RADA16-I nanofiber hydrogels promote osteogenesis,angiogenesis and osteoclastogenesis in vitro and induce multi-functionalized osteoregeneration by intramembranous ossification and bone remodeling in vivo when loaded to collagen(Col)scaffolds.Abundant red blood marrow formation,ideal osteointegration and adapted degradation are observed in the 50%P1R16/Col scaffold group.Therefore,this study provides a promising strategy to develop multi-functional supramolecular peptides and a new method to topically administrate parathyroid hormone or parathyroid hormone related peptides for non-healing bone defects.展开更多
Coronavirus disease 2019(COVID-19)is a highly contagious disease caused by severe acute respiratory syndrome coronavirus 2(SARS-CoV-2).The gold standard method for the diagnosis of SARS-CoV-2 depends on quantitative r...Coronavirus disease 2019(COVID-19)is a highly contagious disease caused by severe acute respiratory syndrome coronavirus 2(SARS-CoV-2).The gold standard method for the diagnosis of SARS-CoV-2 depends on quantitative reverse transcription-polymerase chain reaction till now,which is time-consuming and requires expensive instrumentation,and the confirmation of variants relies on further sequencing techniques.Herein,we first proposed a robust technique-methodology of electrochemical CRISPR sensing with the advantages of rapid,highly sensitivity and specificity for the detection of SARS-CoV-2 variant.To enhance the sensing capability,gold electrodes are uniformly decorated with electro-deposited gold nanoparticles.Using DNA template identical to SARS-CoV-2 Delta spike gene sequence as model,our biosensor exhibits excellent analytical detection limit(50 fM)and high linearity(R2=0.987)over six orders of magnitude dynamic range from 100 fM to 10 nM without any nucleic-acid-amplification assays.The detection can be completed within 1 h with high stability and specificity which benefits from the CRISPR-Cas system.Furthermore,based on the wireless micro-electrochemical platform,the proposed biosensor reveals promising application ability in point-of-care testing.展开更多
Vanadium Alloy is a type of advanced nuclear material with many ideal properties compared as traditional nuclear materials, which has very wide and important application in first-wall and blanket structural material f...Vanadium Alloy is a type of advanced nuclear material with many ideal properties compared as traditional nuclear materials, which has very wide and important application in first-wall and blanket structural material for fusion power plant applications. So it has attracted increasing attentions, especially on new manufacturing methods, such as selective laser melting and so on. In this paper, the comparative study of the powders obtained by mechanical mixing method, dry grinding method and wet grinding method respectively was performed to evaluate the effect of ball milling process on the microstructure and degree of alloying of the vanadium-based powder mixtures with the nominal composition of V5Cr5Ti vanadium alloy. The powders prepared by dry grinding method exhibits better spherical-like morphology and degree of alloying than those prepared by mechanical mixing method and wet grinding method, which indicates that dry grinding method can be used to prepare the superfine vanadium alloy powders for selective laser melting. This work provides a new method as well as important insights into the preparation of superfine vanadium alloy powders for selective laser melting additive manufacturing technology.展开更多
A new structural design for the vent stack with an inclined exit was proposed to reduce the settlement hazard of the cryogenic plume from a cryogenic wind tunnel;it extends the plume trajectory to increase the effecti...A new structural design for the vent stack with an inclined exit was proposed to reduce the settlement hazard of the cryogenic plume from a cryogenic wind tunnel;it extends the plume trajectory to increase the effective contact space and time for mixing between the plume gas and atmospheric air before the plume settles to the ground,contributing to more efficient energy consumption for heating.Reduced-scale experiments and numerical simulations of plume dispersion based on vertical and 30°-and 45°-inclined exits were conducted to study harm reduction and energy-saving potential.Analyses of the minimum temperature and minimum oxygen concentration of the plume near the ground indicate that the new exhaust design with an inclined exit clearly reduces the settlement hazard.Under windless conditions and without using a fan-ejector system,up to 15.9%of the heating energy used by the burner can be saved by adopting the new design.展开更多
Defluoridation of coal mining water is of great significance for sustainable development of coal industry in western China.A novel one-step mechanochemical method was developed to prepare polymeric aluminum modified p...Defluoridation of coal mining water is of great significance for sustainable development of coal industry in western China.A novel one-step mechanochemical method was developed to prepare polymeric aluminum modified powder activated carbon(PAC)for effective fluoride removal from coal mining water.Aluminum was stably loaded on the PAC through facile solid-phase reaction between polymeric aluminum(polyaluminum chloride(PACl)or polyaluminum ferric chloride(PAFC))and PAC(1:15 W/W).Fluoride adsorption on PACl and PAFC modified PAC(C-PACl and C-PAFC)all reached equilibrium within 5 min,at rate of 2.56 g mg^(-1)sec^(-1)and 1.31 g mg^(-1)sec^(-1)respectively.Larger increase of binding energy of Al on C-PACl(Al–F bond:76.64 eV and Al–FOH bond:77.70 eV)relative to that of Al on C-PAFC(Al–F bond:76.52 eV)explained higher fluoride uptake capacity of C-PACl.Less chloride was released from C-PACl than that from C-PAFC due to its higher proportion of covalent chlorine and lower proportion of ionic chlorine.The elements mapping and atomic composition proved the stability of Al loaded on the PAC as well as the enrichment of fluoride on both CPACl and C-PAFC.The Bader charge,formation energy and bond length obtained from DFT computational results explained the fluoride adsorption mechanism further.The carbon emission was 7.73 kg CO_(2)-eq/kg adsorbent prepared through mechanochemical process,which was as low as 1:82.3 to 1:8.07×10^(4)compared with the ones prepared by conventional hydrothermal methods.展开更多
Rationale:Chronic wounds associated with diabetes exact a heavy burden on individuals and society and do not have a specific treatment.Exosome therapy is an extension of stem cell therapy,and RNA interference(RNAi)-ba...Rationale:Chronic wounds associated with diabetes exact a heavy burden on individuals and society and do not have a specific treatment.Exosome therapy is an extension of stem cell therapy,and RNA interference(RNAi)-based therapy is a type of advanced precision therapy.Based on the discovery of chronic wound-related genes in diabetes,we combined exosome therapy and RNAi therapy through an engineering approach for the treatment of diabetic chronic wounds.Methods:We combined exosome therapy and RNAi therapy to establish a precision therapy for diabetes-associated wounds via an engineered exosome approach.Results:First,chronic diabetic wounds express low levels of miR-31-5p compared with nondiabetic wounds,and an miR-31-5p mimic was shown to be effective in promoting the proliferation and migration of three wound-related cell types in vitro.Second,bioinformatics analysis,luciferase reporter assays and western blotting suggested that miR-31-5p promoted angiogenesis,fibrogenesis and reepithelization by inhibiting factor-inhibiting HIF-1(HIF1AN,also named FIH)and epithelial membrane protein-1(EMP-1).Third,engineered miR-31 exosomes were generated as a miR-31-5p RNAi therapeutic agent.In vivo,the engineered miR-31 exosomes promoted diabetic wound healing by enhancing angiogenesis,fibrogenesis and reepithelization.Conclusion:Engineered miR-31 exosomes are an ideal disease pathophysiology-initiated RNAi therapeutic agent for diabetic wounds.展开更多
Importance:The Coronavirus disease 2019(COVID-19)global pandemic poses a considerable challenge for pediatricians.Objective:This study aimed to identify the epidemiological characteristics and clinical features of ped...Importance:The Coronavirus disease 2019(COVID-19)global pandemic poses a considerable challenge for pediatricians.Objective:This study aimed to identify the epidemiological characteristics and clinical features of pediatric patients with COVID-19 in China.Methods:This multicenter retrospective study included pediatric patients from 46 hospitals in China,covering 12 provinces and two municipalities.Epidemiological,demographic,clinical,laboratory,treatment,and outcome data were analyzed.Results:In total,211 pediatric patients with COVID-19 were included in this study.The median age was 7.0 years(range:22 days to 18 years).Approximately 16.3%of the patients exhibited asymptomatic infections,23.0%had upper respiratory tract infections,and 60.7%had pneumonia,including two with severe pneumonia and one with critical illness.Approximately 78.7%of the pediatric patients occurred in familial clusters.The most three common symptoms or signs at onset in children with COVID-19 were fever(54.5%),cough(49.3%),and pharyngeal congestion(20.8%).Only 17.6%of the patients presented with decreased lymphocyte count,whereas 13.6%had increased lymphocyte count.Among the patients with pneumonia who exhibited abnormal chest computed tomography findings,18.2%(23/127)of the patients had no other symptoms.Generally,the chest radiographs showed abnormalities that affected both lungs(49.6%);ground-glass opacity(47.2%)was the most common manifestation.The cure and improvement rates were 86.7%(183/211)and 13.3%(28/211),respectively.Only one patient with an underlying condition received invasive mechanical ventilation;none of the patients died.Interpretation:Similar to adults,children of all age groups are susceptible to COVID-19.Fortunately,most pediatric patients have mild symptoms or remain asymptomatic,despite the high incidence of pneumonia.Decreased proportions of white blood cells and lymphocytes are less frequent in children than in adults.展开更多
Optothermal nanotweezers have emerged as an innovative optical manipulation technique in the past decade,which revolutionized classical optical manipulation by efficiently capturing a broader range of nanoparticles.Ho...Optothermal nanotweezers have emerged as an innovative optical manipulation technique in the past decade,which revolutionized classical optical manipulation by efficiently capturing a broader range of nanoparticles.However,the optothermal temperature field was merely employed for in-situ manipulation of nanoparticles,its potential for identifying bio-nanoparticles remains largely untapped.Hence,based on the synergistic effect of optothermal manipulation and CRIPSR-based bio-detection,we developed CRISPR-powered optothermal nanotweezers(CRONT).Specifically,by harnessing diffusiophoresis and thermo-osmotic flows near the substrate upon optothermal excitation,we successfully trapped and enriched DNA functionalized gold nanoparticles,CRISPR-associated proteins,as well as DNA strands.Remarkably,we built an optothermal scheme for enhancing CRISPR-based single-nucleotide polymorphism(SNP)detection at single molecule level,while also introducing a novel CRISPR methodology for observing nucleotide cleavage.Therefore,this innovative approach has endowed optical tweezers with DNA identification ability in aqueous solution which was unattainable before.With its high specificity and feasibility for in-situ bio-nanoparticle manipulation and identification,CRONT will become a universal tool in point-of-care diagnosis,biophotonics,and bio-nanotechnology.展开更多
基金Innovation Project of Loudi Science and Technology Bureau(Project No.Lou Caijiaozhi(2022)No.2)。
文摘Objective:To explore the correlation between epicardial fat thickness(EFT),aortic velocity propagation(AVP),and abdominal aortic intima-media thickness(AA-IMT)in patients with subclinical hypothyroidism(SH).Additionally,to compare these indicators between SH patients and healthy individuals,providing a new theoretical basis for the clinical prevention and treatment of cardiovascular diseases.Method:Clinical data from 50 SH patients(23 males and 27 females)and 50 healthy outpatient examinees(22 males and 28 females)were analyzed.The participants were selected from January 2022 to December 2023 at Loudi Central Hospital.EFT,AVP,and AA-IMT were measured,and their correlations were analyzed.Results:SH patients had significantly higher EFT and AA-IMT levels than the control group,while their AVP was significantly lower,with these differences being statistically significant(P<0.05).Correlation analysis revealed a significant negative correlation between EFT and AVP(P<0.001),a significant positive correlation between EFT and AAO-IMT(P<0.001),and a significant negative correlation between AVP and AAO-IMT(P<0.001).Multivariate binary logistic regression analysis identified increased EFT,decreased AVP,and increased AAO-IMT as independent risk factors for SH patients.Conclusion:In SH patients,EFT and AAO-IMT are elevated,whereas AVP is reduced.EFT and AVP are significantly correlated with AAO-IMT.EFT and AAO-IMT can serve as reliable indicators for evaluating subclinical atherosclerosis in SH patients,providing a diagnostic basis for clinical practice.
基金financially supported by National Natural Science Foundation of China(No.62005078)the Scientific Research Foundation of Hunan Provincial Education Department(No.21B0477)the Natural Science Foundation of Hunan Province(No.2020JJ5206)。
文摘The detection of manganese(Mn)in steel by laser-induced breakdown spectroscopy(LIBS)provides essential information for steelmaking.However,self-absorption greatly disrupts the LIBS spectral lines of Mn with high content.In this study,to minimize self-absorption for Mn spectral lines in LIBS,laser-induced fluorescence(LIF)was applied.Compared with conventional LIBS,the self-absorption factors(α)of Mn I 403.08,403.31,and 403.45 nm lines were reduced by 90%,88%,and 88%,respectively;the root mean square errors of crossvalidation were decreased by 88%,85%,and 87%,respectively;the average relative errors were reduced by 93%,90%,and 91%,respectively;and average relative standard deviations were decreased by 29%,32%,and 33%,respectively.The LIBS-LIF was shown to successfully minimize the self-absorption effect and spectral intensity fluctuation and improve detection accuracy.
文摘Co–Mo catalysts applied on the hydrodesulfurization（HDS） for FCC gasoline were prepared with Zn–Al layered double hydroxides（LDHs） to improve their performances,and the effects of pore structures and acidity on HDS performances were studied in detail. A series of Zn–Al/LDHs samples with different pore structures and acidities are synthesized on the bases of co-precipitation of OH-,CO2-,Al3＋,and Zn2＋. The neutralization p H is a main factor to affect the pore structures and acidity of Zn–Al/LDHs,and a series of Zn–Al/LDHs with different pore structures and acidities are obtained. Based on the representative samples with different specific surface areas（SBET） and acidities,three Co Mo/LDHs catalysts were prepared,and their HDS performances were compared with traditional Co Mo/Al2O3 catalysts. The results indicated that catalysts prepared with high SBETpossessed high HDS activity,and Br？nsted acid sites could reduce the thiol content in the product to some extent. All the three catalysts prepared with LDHs displayed little lower HDS activity but higher selectivity than Co Mo/Al2O3,and could restrain the reactions of re-combination between olefin and H2 S which could be due to the existence of Br？nsted acid sites.
基金supported by the National Program on Key Basic Research Project ("973 Program")under grant No.2007CB607505the National High Technology Research and Development Program of China under grant No.2009AAO3Z216
文摘Polycrystalline La-doped CaMnO3 ceramics have been prepared by a solid-state sintering method. Analysis of microstructure and phase composition indicates that the addition of La can prohibit the further growth of grain, and no impurity phase appears. The results revealed that the La doping can lead to a large change of the activation energy （from 0.2：2 to 0.02 eV）, and thus result in a marked increase in electric conductivity of 2-4 orders of magnitude. The power factor can reach about 1.5×10-4 W.m-1.K-2 in a wide temperature range, which potentially make them attractive for n-type high-temperature thermoelectric materials.
基金This research was supported by the Open Fund Project of the State Key Laboratory of Water Resources Protection and Utilization in Coal Mining(GJNY-18-73.13).
文摘Membrane distillation(MD)is a promising membrane separation technique used to treat industrial wastewater.When coupled with cheap heat sources,MD has significant economic advantages.Therefore,MD can be combined with solar energy to realize the large-scale and low-cost treatment of highly mineralized mine water in the western coalproducing region of China.In this study,highly mineralized mine water from the Ningdong area of China was subjected to vacuum MD(VMD)using polyvinylidene fluoride hollow-fiber membranes.The optimal operation parameters of VMD were determined by response surface optimization.Subsequently,the feasibility of VMD for treating highly mineralized mine water was explored.The fouling behavior observed during VMD was further investigated by scanning electron microscopy with energy-dispersive X-ray spectroscopy(SEM-EDS).Under the optimal parameters(pressure=-0.08 MPa,temperature=70℃,and feed flow rate=1.5 L/min),the maximum membrane flux was 8.85 kg/(m^(2) h),and the desalination rate was 99.7%.Membrane fouling could be divided into three stages:membrane wetting,crystallization,and fouling layer formation.Physical cleaning restored the flux and salt rejection rate to 94%and 97%of the initial values,respectively;however,the cleaning interval and cleaning efficiency decreased as the VMD run time increased.SEM-EDS analysis revealed that the reduction in flux was caused by the precipitation of CaCO_(3).The findings also demonstrated that the membrane wetting could be attributed to the formation of NaCl on the cross section and outer surface of the membrane.Overall,the results confirm the feasibility of MD for treating mine water and provide meaningful guidance for the industrial application of MD.
基金supported by the National Natural Science Foundation of China(No.82372405,No.81871752)the Fundamental Research Funds for the Central Universities(NO.2042023kf0199)+2 种基金the Key Research and Development Program of Hubei Province(No:2022BCA052)the Key Research and Development Program of Wuhan City(No.2023020402010591)the Translational Medicine and Interdisciplinary Research Joint Fund of Zhongnan Hospital of Wuhan University(No.ZNJC202014).
文摘Supramolecular peptide nanofiber hydrogels are emerging biomaterials for tissue engineering,but it is difficult to fabricate multi-functional systems by simply mixing several short-motif-modified supramolecular peptides because relatively abundant motifs generally hinder nanofiber cross-linking or the formation of long nanofiber.Coupling bioactive factors to the assembling backbone is an ideal strategy to design multi-functional supramolecular peptides in spite of challenging synthesis and purification.Herein,a multi-functional supramolecular peptide,P1R16,is developed by coupling a bioactive factor,parathyroid hormone related peptide 1(PTHrP-1),to the basic supramolecular peptide RADA16-I via solid-phase synthesis.It is found that P1R16 self-assembles into long nanofibers and co-assembles with RADA16-I to form nanofiber hydrogels,thus coupling PTHrP-1 to hydrogel matrix.P1R16 nanofiber retains osteoinductive activity in a dose-dependent manner,and P1R16/RADA16-I nanofiber hydrogels promote osteogenesis,angiogenesis and osteoclastogenesis in vitro and induce multi-functionalized osteoregeneration by intramembranous ossification and bone remodeling in vivo when loaded to collagen(Col)scaffolds.Abundant red blood marrow formation,ideal osteointegration and adapted degradation are observed in the 50%P1R16/Col scaffold group.Therefore,this study provides a promising strategy to develop multi-functional supramolecular peptides and a new method to topically administrate parathyroid hormone or parathyroid hormone related peptides for non-healing bone defects.
基金support from the Innovation Team Project of Department of Education of Guangdong Province(No.2018KCXTD026)Guangdong Scientific and Technological Project(2019B1515120043,2020A151501612,2021A1515220109,2022B1515020093)+1 种基金Science and Technology Innovation Commission of Shenzhen(Grant No.KCXFZ20201221173413038)Longhua District Science and Innovation Commission Project Grants of Shenzhen(JCYJ201904).
文摘Coronavirus disease 2019(COVID-19)is a highly contagious disease caused by severe acute respiratory syndrome coronavirus 2(SARS-CoV-2).The gold standard method for the diagnosis of SARS-CoV-2 depends on quantitative reverse transcription-polymerase chain reaction till now,which is time-consuming and requires expensive instrumentation,and the confirmation of variants relies on further sequencing techniques.Herein,we first proposed a robust technique-methodology of electrochemical CRISPR sensing with the advantages of rapid,highly sensitivity and specificity for the detection of SARS-CoV-2 variant.To enhance the sensing capability,gold electrodes are uniformly decorated with electro-deposited gold nanoparticles.Using DNA template identical to SARS-CoV-2 Delta spike gene sequence as model,our biosensor exhibits excellent analytical detection limit(50 fM)and high linearity(R2=0.987)over six orders of magnitude dynamic range from 100 fM to 10 nM without any nucleic-acid-amplification assays.The detection can be completed within 1 h with high stability and specificity which benefits from the CRISPR-Cas system.Furthermore,based on the wireless micro-electrochemical platform,the proposed biosensor reveals promising application ability in point-of-care testing.
文摘Vanadium Alloy is a type of advanced nuclear material with many ideal properties compared as traditional nuclear materials, which has very wide and important application in first-wall and blanket structural material for fusion power plant applications. So it has attracted increasing attentions, especially on new manufacturing methods, such as selective laser melting and so on. In this paper, the comparative study of the powders obtained by mechanical mixing method, dry grinding method and wet grinding method respectively was performed to evaluate the effect of ball milling process on the microstructure and degree of alloying of the vanadium-based powder mixtures with the nominal composition of V5Cr5Ti vanadium alloy. The powders prepared by dry grinding method exhibits better spherical-like morphology and degree of alloying than those prepared by mechanical mixing method and wet grinding method, which indicates that dry grinding method can be used to prepare the superfine vanadium alloy powders for selective laser melting. This work provides a new method as well as important insights into the preparation of superfine vanadium alloy powders for selective laser melting additive manufacturing technology.
基金supported by the Key Research and Development Program of Zhejiang Province(No.2020C01029),China。
文摘A new structural design for the vent stack with an inclined exit was proposed to reduce the settlement hazard of the cryogenic plume from a cryogenic wind tunnel;it extends the plume trajectory to increase the effective contact space and time for mixing between the plume gas and atmospheric air before the plume settles to the ground,contributing to more efficient energy consumption for heating.Reduced-scale experiments and numerical simulations of plume dispersion based on vertical and 30°-and 45°-inclined exits were conducted to study harm reduction and energy-saving potential.Analyses of the minimum temperature and minimum oxygen concentration of the plume near the ground indicate that the new exhaust design with an inclined exit clearly reduces the settlement hazard.Under windless conditions and without using a fan-ejector system,up to 15.9%of the heating energy used by the burner can be saved by adopting the new design.
基金supported by the National Natural Science Foundation of China(No.52100070)the Science and Technology Innovation Project of China Energy Investment Corporation(No.SZY93002219N).
文摘Defluoridation of coal mining water is of great significance for sustainable development of coal industry in western China.A novel one-step mechanochemical method was developed to prepare polymeric aluminum modified powder activated carbon(PAC)for effective fluoride removal from coal mining water.Aluminum was stably loaded on the PAC through facile solid-phase reaction between polymeric aluminum(polyaluminum chloride(PACl)or polyaluminum ferric chloride(PAFC))and PAC(1:15 W/W).Fluoride adsorption on PACl and PAFC modified PAC(C-PACl and C-PAFC)all reached equilibrium within 5 min,at rate of 2.56 g mg^(-1)sec^(-1)and 1.31 g mg^(-1)sec^(-1)respectively.Larger increase of binding energy of Al on C-PACl(Al–F bond:76.64 eV and Al–FOH bond:77.70 eV)relative to that of Al on C-PAFC(Al–F bond:76.52 eV)explained higher fluoride uptake capacity of C-PACl.Less chloride was released from C-PACl than that from C-PAFC due to its higher proportion of covalent chlorine and lower proportion of ionic chlorine.The elements mapping and atomic composition proved the stability of Al loaded on the PAC as well as the enrichment of fluoride on both CPACl and C-PAFC.The Bader charge,formation energy and bond length obtained from DFT computational results explained the fluoride adsorption mechanism further.The carbon emission was 7.73 kg CO_(2)-eq/kg adsorbent prepared through mechanochemical process,which was as low as 1:82.3 to 1:8.07×10^(4)compared with the ones prepared by conventional hydrothermal methods.
基金The authors acknowledge the support of the National Natural Science Foundation of China(81572178,81270397,81702317,81871752 and 81770802)the National Key R&D Program of China(2017YFC1309601 to Fang Liu)+1 种基金Shanghai Municipal Commission of Health and Family Planning under the fund(20124356)a Shanghai Municipal Education Commission-Gaofeng Clinical Medicine Grant(20152232).
文摘Rationale:Chronic wounds associated with diabetes exact a heavy burden on individuals and society and do not have a specific treatment.Exosome therapy is an extension of stem cell therapy,and RNA interference(RNAi)-based therapy is a type of advanced precision therapy.Based on the discovery of chronic wound-related genes in diabetes,we combined exosome therapy and RNAi therapy through an engineering approach for the treatment of diabetic chronic wounds.Methods:We combined exosome therapy and RNAi therapy to establish a precision therapy for diabetes-associated wounds via an engineered exosome approach.Results:First,chronic diabetic wounds express low levels of miR-31-5p compared with nondiabetic wounds,and an miR-31-5p mimic was shown to be effective in promoting the proliferation and migration of three wound-related cell types in vitro.Second,bioinformatics analysis,luciferase reporter assays and western blotting suggested that miR-31-5p promoted angiogenesis,fibrogenesis and reepithelization by inhibiting factor-inhibiting HIF-1(HIF1AN,also named FIH)and epithelial membrane protein-1(EMP-1).Third,engineered miR-31 exosomes were generated as a miR-31-5p RNAi therapeutic agent.In vivo,the engineered miR-31 exosomes promoted diabetic wound healing by enhancing angiogenesis,fibrogenesis and reepithelization.Conclusion:Engineered miR-31 exosomes are an ideal disease pathophysiology-initiated RNAi therapeutic agent for diabetic wounds.
基金This study was supported by CAMS Innovation Fund for Medical Sciences(CIFMS,2019-12M-5-0262020-I2M-C&T-B-098).
文摘Importance:The Coronavirus disease 2019(COVID-19)global pandemic poses a considerable challenge for pediatricians.Objective:This study aimed to identify the epidemiological characteristics and clinical features of pediatric patients with COVID-19 in China.Methods:This multicenter retrospective study included pediatric patients from 46 hospitals in China,covering 12 provinces and two municipalities.Epidemiological,demographic,clinical,laboratory,treatment,and outcome data were analyzed.Results:In total,211 pediatric patients with COVID-19 were included in this study.The median age was 7.0 years(range:22 days to 18 years).Approximately 16.3%of the patients exhibited asymptomatic infections,23.0%had upper respiratory tract infections,and 60.7%had pneumonia,including two with severe pneumonia and one with critical illness.Approximately 78.7%of the pediatric patients occurred in familial clusters.The most three common symptoms or signs at onset in children with COVID-19 were fever(54.5%),cough(49.3%),and pharyngeal congestion(20.8%).Only 17.6%of the patients presented with decreased lymphocyte count,whereas 13.6%had increased lymphocyte count.Among the patients with pneumonia who exhibited abnormal chest computed tomography findings,18.2%(23/127)of the patients had no other symptoms.Generally,the chest radiographs showed abnormalities that affected both lungs(49.6%);ground-glass opacity(47.2%)was the most common manifestation.The cure and improvement rates were 86.7%(183/211)and 13.3%(28/211),respectively.Only one patient with an underlying condition received invasive mechanical ventilation;none of the patients died.Interpretation:Similar to adults,children of all age groups are susceptible to COVID-19.Fortunately,most pediatric patients have mild symptoms or remain asymptomatic,despite the high incidence of pneumonia.Decreased proportions of white blood cells and lymphocytes are less frequent in children than in adults.
基金supported by the National Natural Science Foundation of China(62275164,61905145,62275168,61775148)National Key Research and Development Program of China(No.2022YFA1206300)+8 种基金Guangdong Natural Science Foundation and Province Project(2021A1515011916,2023A1515012250)Foundation from Department of Science and Technology of Guangdong Province(2021QN02Y124)Shenzhen Science and Technology R&D and Innovation Foundation(JCYJ20200109105608771)Shenzhen Key Laboratory of Photonics and Biophotonics(ZDSYS20210623092006020)The Research Grants Council(RGC)of Hong Kong China(RGC14207920)Innovation Team Project of Department of Education of Guangdong Province(2018KCXTD026)Deanship of Scientific Research(DSR)at King Abdulaziz University,Jeddah(KEP-MSc-70-130-42)King Khalid University through Research Center for Advanced Materials Science(RCAMS)(RCAMS/KKU/006/21)Medical-Engineering Interdisciplinary Research Foundation of Shenzhen University。
文摘Optothermal nanotweezers have emerged as an innovative optical manipulation technique in the past decade,which revolutionized classical optical manipulation by efficiently capturing a broader range of nanoparticles.However,the optothermal temperature field was merely employed for in-situ manipulation of nanoparticles,its potential for identifying bio-nanoparticles remains largely untapped.Hence,based on the synergistic effect of optothermal manipulation and CRIPSR-based bio-detection,we developed CRISPR-powered optothermal nanotweezers(CRONT).Specifically,by harnessing diffusiophoresis and thermo-osmotic flows near the substrate upon optothermal excitation,we successfully trapped and enriched DNA functionalized gold nanoparticles,CRISPR-associated proteins,as well as DNA strands.Remarkably,we built an optothermal scheme for enhancing CRISPR-based single-nucleotide polymorphism(SNP)detection at single molecule level,while also introducing a novel CRISPR methodology for observing nucleotide cleavage.Therefore,this innovative approach has endowed optical tweezers with DNA identification ability in aqueous solution which was unattainable before.With its high specificity and feasibility for in-situ bio-nanoparticle manipulation and identification,CRONT will become a universal tool in point-of-care diagnosis,biophotonics,and bio-nanotechnology.
