Ever since the low energy N + ion beam has been accepted, the mutations of ionizing radiation are attributable mainly to avoidance of DNA damages repair. Evidences based on in vivo proof results are limited. Using the...Ever since the low energy N + ion beam has been accepted, the mutations of ionizing radiation are attributable mainly to avoidance of DNA damages repair. Evidences based on in vivo proof results are limited. Using the E.coli wild type and mutator strains, the mutant frequencies suggest that base substitutions in rpoB gene are induced by the N + implantation. A highly conserved region is selected to get the direct evidence for base substitutions by sequence of the high fidelity PCR amplification products in mutants. Most of the mutants (90.9%, 40/44) have at least one base substitution in the amplification region. The evidences for CG to TA (55%, 22/40), AT to GC (20%, 8/40) and TA to CG (5%, 2/40) transitions are identified. The transversions are AT to TA (15%, 6/40) and GC to CG (5%, 2/40). It is suggested that DNA cytosine methylase might play an important role in mismatch repair of DNA damage induced by N + implantation by analysis of the mutant frequencies of mutator strains.展开更多
采用蛋白质组学寻找可能与支气管哮喘(bronchial asthma, BA)致病相关的关键蛋白。首先收集健康成年人与哮喘患者的血清样本,运用去除高丰度结构和非特异性蛋白质后的蛋白定量质谱(Tandem Mass TagTM,TMT),分别获得哮喘患者与健康成年...采用蛋白质组学寻找可能与支气管哮喘(bronchial asthma, BA)致病相关的关键蛋白。首先收集健康成年人与哮喘患者的血清样本,运用去除高丰度结构和非特异性蛋白质后的蛋白定量质谱(Tandem Mass TagTM,TMT),分别获得哮喘患者与健康成年人差异表达蛋白,并进行GO富集和KEGG富集分析,通过蛋白相互作用(PPI)网络分析筛选出哮喘组中的核心蛋白。然后将核心蛋白在3例支气管哮喘患者和3例健康成年人中进行蛋白印迹(Western blot)验证。通过蛋白质组学筛查共鉴定出778个差异表达蛋白,其中32个蛋白质包含定量信息(18个蛋白表达上调、14个蛋白表达下调);KEGG富集分析具有明显表达差异的信号通路有28条。PPI结果显示有10个蛋白(GDN、1433Z、VWF、HEMO、CERU、A1AT、TSP1、G3P、IBP7、KPYM)可能在支气管哮喘的发病机制中扮演重要作用。而相对于健康成年人,支气管哮喘患者血清中SLC25A4、SVEP1和KRT25蛋白表达水平升高,差异均具有统计学意义(P<0.05)。因此,推测多种免疫信号通路及差异表达蛋白在BA的发病机制中扮演了重要作用,为BA的治疗靶点研究进一步提供了方向。展开更多
目的探索经颅直流电刺激（tDCS）干预对精神分裂症患者认知功能的作用。方法纳入具有认知功能损害的精神分裂症住院患者39例，随机分为刺激组和伪刺激组。两组疗程均为连续10个工作日，每日进行一次tDCS干预。电极阳极置于患者左侧背外...目的探索经颅直流电刺激（tDCS）干预对精神分裂症患者认知功能的作用。方法纳入具有认知功能损害的精神分裂症住院患者39例，随机分为刺激组和伪刺激组。两组疗程均为连续10个工作日，每日进行一次tDCS干预。电极阳极置于患者左侧背外侧前额叶区，阴极置于右侧眶上缘区，电流强度2mA，干预时间20min。研究期间患者均接受常规治疗。采用中文版MATRICS认知成套测验（MCCB）、节奏听觉串行加法任务（PASAT）、CogState成套测验（Cog State Battery）、“看眼读心”测验（RMET）进行治疗基线和干预结束后4周的认知功能评定。结果除社会认知外，刺激组在各领域分值及MCCB总分上均高于对照组，但是仅在信息处理速度上组间差异有统计学意义（F=4．087，P=0．051）。刺激组中MCCB总分改善的患者的基线认知功能显著优于未改善患者（Z=0．085，P=0．011）。结论tDCS对信息处理速度的调控上具有一定维持作用，但仍需要大样本研究进一步证实。展开更多
文摘Ever since the low energy N + ion beam has been accepted, the mutations of ionizing radiation are attributable mainly to avoidance of DNA damages repair. Evidences based on in vivo proof results are limited. Using the E.coli wild type and mutator strains, the mutant frequencies suggest that base substitutions in rpoB gene are induced by the N + implantation. A highly conserved region is selected to get the direct evidence for base substitutions by sequence of the high fidelity PCR amplification products in mutants. Most of the mutants (90.9%, 40/44) have at least one base substitution in the amplification region. The evidences for CG to TA (55%, 22/40), AT to GC (20%, 8/40) and TA to CG (5%, 2/40) transitions are identified. The transversions are AT to TA (15%, 6/40) and GC to CG (5%, 2/40). It is suggested that DNA cytosine methylase might play an important role in mismatch repair of DNA damage induced by N + implantation by analysis of the mutant frequencies of mutator strains.
基金国家建设高水平大学公派研究生项目（CSC201606230115） 感谢John M.Davis Family Foundation对本课题的资助
文摘目的探索经颅直流电刺激（tDCS）干预对精神分裂症患者认知功能的作用。方法纳入具有认知功能损害的精神分裂症住院患者39例，随机分为刺激组和伪刺激组。两组疗程均为连续10个工作日，每日进行一次tDCS干预。电极阳极置于患者左侧背外侧前额叶区，阴极置于右侧眶上缘区，电流强度2mA，干预时间20min。研究期间患者均接受常规治疗。采用中文版MATRICS认知成套测验（MCCB）、节奏听觉串行加法任务（PASAT）、CogState成套测验（Cog State Battery）、“看眼读心”测验（RMET）进行治疗基线和干预结束后4周的认知功能评定。结果除社会认知外，刺激组在各领域分值及MCCB总分上均高于对照组，但是仅在信息处理速度上组间差异有统计学意义（F=4．087，P=0．051）。刺激组中MCCB总分改善的患者的基线认知功能显著优于未改善患者（Z=0．085，P=0．011）。结论tDCS对信息处理速度的调控上具有一定维持作用，但仍需要大样本研究进一步证实。
