[Objectives]Using wild-type AB strain of zebrafish as experimental animal,this study investigated the damaging effect of Wufang Babu Ointment on skin cells,in order to evaluate the skin toxicity of Wufang Babu Ointmen...[Objectives]Using wild-type AB strain of zebrafish as experimental animal,this study investigated the damaging effect of Wufang Babu Ointment on skin cells,in order to evaluate the skin toxicity of Wufang Babu Ointment.[Methods]Wild-type AB strain of zebrafish with an age of 2 d were taken and fed in different concentrations of Wufang Babu Ointment solution for 24 h.The number of deaths in each group of zebrafish was recorded,and the mortality rate was calculated.Using Origin 8.0 software,the maximum non lethal concentration(MNLC)was simulated.Zebrafish raised in different concentrations of Wufang Babu Ointment solution for 24 h were placed under an anatomical microscope for taking photos,to analyze and calculate the incidence of skin damage in zebrafish.Based on the statistical analysis results of this indicator,the skin toxicity of Wufang Babu Ointment was evaluated.[Results]The MNLC of Wufang Babu Ointment on zebrafish was 671μg/mL;Wufang Babu Ointment can induce skin damage at the concentrations of 224μg/mL(1/3 MNLC)and 671μg/mL(MNLC).[Conclusions]Wufang Babu Ointment had certain skin toxicity to zebrafish.展开更多
The synthesis of oxygen vacancies(OVs)-modified TiO_(2)under mild conditions is attractive.In this work,OVs were easily introduced in TiO_(2)lattice during the hydrothermal doping process of trivalent iron ions.Theore...The synthesis of oxygen vacancies(OVs)-modified TiO_(2)under mild conditions is attractive.In this work,OVs were easily introduced in TiO_(2)lattice during the hydrothermal doping process of trivalent iron ions.Theoretical calculations based on a novel charge-compensation structure model were employed with experimental methods to reveal the intrinsic photocatalytic mechanism of Fe-doped TiO_(2)(Fe-TiO_(2)).The OVs formation energy in Fe-TiO_(2)(1.12 eV)was only 23.6%of that in TiO_(2)(4.74 eV),explaining why Fe^(3+)doping could introduce OVs in the TiO_(2)lattice.The calculation results also indicated that impurity states introduced by Fe^(3+)and OVs enhanced the light absorption activity of TiO_(2).Additionally,charge carrier transport was investigated through the carrier lifetime and relative mass.The carrier lifetime of Fe-TiO_(2)(4.00,4.10,and 3.34 ns for 1at%,2at%,and 3at%doping contents,respectively)was longer than that of undoped TiO_(2)(3.22 ns),indicating that Fe^(3+) and OVs could promote charge carrier separation,which can be attributed to the larger relative effective mass of electrons and holes.Herein,Fe-TiO_(2)has higher photocatalytic indoor NO removal activity compared with other photocatalysts because it has strong light absorption activity and high carrier separation efficiency.展开更多
Aging, subjected to scientific scrutiny, is extensively defined as a time-dependent decline in functions that involves the majority of organisms. The time-dependent accretion of cellular lesions is generally a univers...Aging, subjected to scientific scrutiny, is extensively defined as a time-dependent decline in functions that involves the majority of organisms. The time-dependent accretion of cellular lesions is generally a universal trigger of aging, while mitochondrial dysfunction is a sign of aging. Dysfunctional mitochondria are identified and removed by mitophagy, a selective form of macroautophagy. Increased mitochondrial damage resulting from reduced biogenesis and clearance may promote the aging process. The primary purpose of this paper is to illustrate in detail the effects of mitophagy on aging and emphasize the associations between mitophagy and other signs of aging, including dietary restriction, telomere shortening, epigenetic alterations, and protein imbalance.The evidence regarding the effects of these elements on aging is still limited. And although the understanding of relationship between mitophagy and aging has been long-awaited, to analyze details of such a relationship remains the main challenge in aging studies.展开更多
[Objectives]The paper was to establish the quality standard of Shenqi Qiangjing granules and to conduct routine inspection of granular dosage forms.[Methods]The particle size,moisture,loss on drying,dissolubility,cont...[Objectives]The paper was to establish the quality standard of Shenqi Qiangjing granules and to conduct routine inspection of granular dosage forms.[Methods]The particle size,moisture,loss on drying,dissolubility,content uniformity and microbial limit of Shenqi Qiangjing granules were detected.Radix Astragali,Herba Epimedii,Radix Dipsaci and Fructus Schisandrae Chinensis were qualitatively identified by thin layer chromatography(TLC).[Results]The particle size,moisture,loss on drying,dissolubility,content uniformity and microbial limit of Shenqi Qiangjing granules were all in line with the relevant regulations of Pharmacopoeia of the People's Republic of China(2020 edition).Radix Astragali,Herba Epimedii,Radix Dipsaci and Fructus Schisandrae Chinensis had clear fluorescent spots in TLC assay,and no such spots were found in negative control.[Conclusions]The method has the advantages of simple operation,accurate and reliable results,strong specificity and good repeatability,and can effectively control the quality of Shenqi Qiangjing granules.展开更多
[Objectives] To identify the species of Hirudo from different sources. [Methods] In accordance with morphological, anatomical and molecular taxonomic characteristics, the identification conclusion was drawn. [Results]...[Objectives] To identify the species of Hirudo from different sources. [Methods] In accordance with morphological, anatomical and molecular taxonomic characteristics, the identification conclusion was drawn. [Results] The living samples of Hirudo belong to 1 order, 2 families, 3 genera and 5 species, and the dry samples of medicinal leeches belong to 4 species. [Conclusions] The sources of medicinal leeches are complex, and there is a problem that Hirudo from different basic sources are mixed for medication.展开更多
[Objectives]To establish the quality standard of Fuyang Wenshen Granules and carry out the routine examination of granule type.[Methods]The particle size,water content,solubility,loading difference and microbial limit...[Objectives]To establish the quality standard of Fuyang Wenshen Granules and carry out the routine examination of granule type.[Methods]The particle size,water content,solubility,loading difference and microbial limit of Fuyang Wenshen Granules were examined,and TLC was used for qualitative identification of Astmgali Radix and Notoginseng Radix Et Rhizoma.[Results]The particle size,water content,solubility,loading difference,and microbial limit of Fuyang Wenshen Granules complied with the relevant provisions of the Pharmacopoeia of the People's Republic of China(2020).The TLC spots of Astmgali Radix and Notoginseng Radix Et Rhizoma were clearly colored,and negative had no interference.[Conclusions]The method was simple,accurate,reliable,specific and reproducible,and can effectively control the quality of Fuyang Wenshen Granules.展开更多
Convenient,rapid,and accurate detection of cardiac troponin I(cTnI)is crucial in early diagnosis of acute myocardial infarction(AMI).A paper-based electrochemical immunosensor is a promising choice in this field,becau...Convenient,rapid,and accurate detection of cardiac troponin I(cTnI)is crucial in early diagnosis of acute myocardial infarction(AMI).A paper-based electrochemical immunosensor is a promising choice in this field,because of the flexibility,porosity,and cost-efficacy of the paper.However,paper is poor in electronic conductivity and surface functionality.Herein,we report a paper-based electrochemical immunosensor for the label-free detection of cTnI with the working electrode modified by MXene(Ti_(3)C_(2))nanosheets.In order to immobilize the bio-receptor(anti-cTnI)on the MXene-modified working electrode,the MXene nanosheets were functionalized by aminosilane,and the functionalized MXene was immobilized onto the surface of the working electrode through Nafion.The large surface area of the MXene nanosheets facilitates the immobilization of antibodies,and the excellent conductivity facilitates the electron transfer between the electrochemical species and the underlying electrode surface.As a result,the paper-based immunosensor could detect cTnI within a wide range of 5-100 ng/mL with a detection limit of 0.58 ng/mL.The immunosensor also shows outstanding selectivity and good repeatability.Our MXene-modified paper-based electrochemical immunosensor enables fast and sensitive detection of cTnI,which may be used in real-time and cost-efficient monitoring of AMI diseases in clinics.展开更多
Coalbed methane(CBM)drilling and completion technologies(DCTs)are signifcant basis for achieving efcient CBM exploration and exploitation.Characteristics of CBM reservoirs vary in diferent regions around the world,the...Coalbed methane(CBM)drilling and completion technologies(DCTs)are signifcant basis for achieving efcient CBM exploration and exploitation.Characteristics of CBM reservoirs vary in diferent regions around the world,thereby,it is crucial to develop,select and apply the optimum DCTs for each diferent CBM reservoir.This paper frstly reviews the development history of CBM DCTs throughout worldwide and clarifes its overall development tendency.Secondly,diferent well types and its characteristics of CBM exploitation are summarized,and main application scopes of these well types are also discussed.Then,the key technologies of CBM drilling(directional drilling tools,measurement while drilling,geo-steering drilling,magnetic guidance drilling,underbalanced drilling and drilling fuids),and the key technologies of CBM completion(open-hole,cavity and under-ream completion,cased-hole completion,screen pipe completion and horizontal well completion)are summarized and analyzed,it is found that safe,economic and efcient development of CBM is inseparable from the support of advanced technologies.Finally,based on the current status of CBM development,the achievements,existing challenges and future prospects are summarized and discussed from the perspective of CBM DCTs.展开更多
Bone-like nanohydroxyapatite powders (b-nanoHA) were synthesized in simulated body fluid (SBF). The b-nanoHA, gelatin (Gel) and Polyvinyl Alcohol (PVA) were used to prepare bone-like composites (b-nanoHA/ Gel/PVA) at ...Bone-like nanohydroxyapatite powders (b-nanoHA) were synthesized in simulated body fluid (SBF). The b-nanoHA, gelatin (Gel) and Polyvinyl Alcohol (PVA) were used to prepare bone-like composites (b-nanoHA/ Gel/PVA) at room temperature. Characterizations of b-nanoHA powders and b-nanoHA/Gel/PVA composites were investigated by using X-ray diffraction (XRD), transmission electron microscopy (TEM), High-resolution transmission electron microscopy (HRTEM), scanning electron microscopy (SEM) and Fourier transform infrared spectroscopy (FT-IR). Bending strength and compressive strength of the composite were tested. It was found that microstructure of the b-nanoHA powders was whisker shape and its crystalline degree was low similar to natural bone, bending strength and compressive strength of the b-nanoHA/Gel/PVA composite depended on the mixing ratio of HA, Gel and PVA, and also PVA could induce the network formation in the b-nanoHA/Gel/ PVA composite.展开更多
This article reviews the species,identification,chemical composition and pharmacological effects of hirudo.Hirudo is widely sourced,difficult to identify,contains a variety of active ingredients,and has a variety of p...This article reviews the species,identification,chemical composition and pharmacological effects of hirudo.Hirudo is widely sourced,difficult to identify,contains a variety of active ingredients,and has a variety of pharmacological effects.In-depth research on hirudo is of great significance to the quality control and clinical application of hirudo.展开更多
At present,the education of specialized courses in science and engineering still focuses on imparting specialized knowledge,which seriously lacks the embodiment of educating students.Taking the specialized course“Sol...At present,the education of specialized courses in science and engineering still focuses on imparting specialized knowledge,which seriously lacks the embodiment of educating students.Taking the specialized course“Solid Luminescent Materials”as an example,the new mode of ideological/political teaching is integrated into the specialized course,giving full play to the role of the main position of the specialized class,so as to realize the aim of teaching specialized knowledge in cooperation with educating students and imperceptibly influencing the ideological/political teaching.In this paper,the design of ideological/political knowledge points and the integration of ideological/political cases are carried out from the aspects of teaching concepts,teaching contents and teaching cases.By adhering to the cooperation between moral and financial classroom and specialized education,the educational function of university specialized courses can be effectively brought into play,which is expected to guide students to enhance their awareness of energy conservation,environmental protection,innovation and patriotism.展开更多
[Objectives] This study was conducted to clarify the roles of macronutrients in regulating plant primary and secondary metabolism of Scutellaria baicalensis Georgi. [Methods] The contents of chlorophyll(Chl) and solub...[Objectives] This study was conducted to clarify the roles of macronutrients in regulating plant primary and secondary metabolism of Scutellaria baicalensis Georgi. [Methods] The contents of chlorophyll(Chl) and soluble sugar were detected with ultraviolet spectrophotometry. The activities of phenylalanine ammonia-lyase(PAL), cinnamate 4-hydroxylase(C_4H) and chalcone synthase(CHS) were determined with ultraviolet spectrophotometry. Secondary metabolites were detected by high performance liquid chromatography(HPLC). [Results] The content of chlorophyll in treatments N, P and K was increased, showing significant difference from that of the control(P<0.05). Among them, Treatment N has the highest content of chlorophyll. Soluble sugar content in treatments N, P and K(K>N>P>CK) was increased considerably and had significant difference from that of the control(P<0.05). Compared with the control, PAL and C_4H activity in treatments N, P and K were increased dramatically by 186.57%, 134.09%, 306.91% and 73.21%, 28.91%, 247.57%, respectively. CHS activity was also increased sharply in treatments N, P and K. Scutellarin, baicalin, wogonoside, baicalein, wogonin and oroxylin A contents in all treatments N, P and K(K>N>P) were increased, and had significant difference from that of the control(P<0.05). [Conclusions] The results showed that macronutrients can increase leaf chlorophyll content, soluble sugar content, PAL activity, C_4H activity and secondary metabolites of S. baicalensis.展开更多
[Objectives]This study was conducted to study the Germination characteristics and secondary metabolism regulation of Scutellaria baicalensis Georgi under osmotic stress.[Methods]The activity of praline,soluble sugar a...[Objectives]This study was conducted to study the Germination characteristics and secondary metabolism regulation of Scutellaria baicalensis Georgi under osmotic stress.[Methods]The activity of praline,soluble sugar and protein was detected with ultraviolet spectrophotometry.The activity of phenylalanine ammonia-lyase(PAL)and cinnamic acid 4-hydroxylase(C_4H)was determined with ultraviolet spectrophotometry.The secondary metabolites were detected by high performance liquid chromatography(HPLC).[Results]The germination percentage,germination potentiality and germination index decreased at first and increased subsequently along with the enhancement of PEG concentration.The praline content,the soluble sugar content and the soluble protein content in S.baicalensis seedlings increased along with the enhancement of PEG concentration and the differences from the CK were significant(P<0.05).The PAL and C_4H in S.baicalensis seedlings decreased after osmotic stress and the differences from the CK were significant(P<0.05).The results indicate that,the scutellarin,baicalin,wogonoside,baicalein,wogonin and oroxylinA contents decreased after osmotic stress and the differences from the CK were significant(P<0.05).[Conclusions]The quality of the skullcaps could be improved by improving the activity of PAL and C_4H.展开更多
[Objectives]The content of total anthraquinone and 5 free anthraquinones in different processed products of rhubarb were determined.[Methods]Using emodin as the reference,the concentration of 1%magnesium acetate metha...[Objectives]The content of total anthraquinone and 5 free anthraquinones in different processed products of rhubarb were determined.[Methods]Using emodin as the reference,the concentration of 1%magnesium acetate methanol solution was used for color development,and the absorbance was measured at 516 nm wavelength;the mobile phase was methanol-0.1%phosphoric acid(76∶24)aqueous solution,the detection wavelength was set at 256 nm,the flow rate was 1.0 mL/min,the column temperature was 30℃,and the sample size was 10μL.[Results]The content of total anthraquinones in rhubarb was determined by UV spectrophotometry.The linear range was 0.98-23.52μg/mL,R2=0.9993,the average recoveries were 100.12%,99.66%and 99.15%,with RSDs of 1.58%,0.94%and 1.41%,respectively.The linear ranges of emodin,emodin,emodin,chrysophanol and emodin methyl ether were 0.0207-0.414(R2=0.9999),0.1721-3.442(R2=0.9996),0.0203-0.406(R2=0.9999),0.148-2.96(R2=0.9998)and 0.164-3.28(R2=0.9993),respectively.[Conclusions]The contents of total anthraquinones and free anthraquinones in different processed products of rhubarb were determined by UV spectrophotometry and HPLC.The method can be used for quality control of rhubarb and its different processed products.展开更多
[Objectives]To study the long-term toxicity of Maxing Erchen Zhike granules to rats after intragastric administration,so as to provide reference for its preclinical safety evaluation.[Methods]Total 80 rats were random...[Objectives]To study the long-term toxicity of Maxing Erchen Zhike granules to rats after intragastric administration,so as to provide reference for its preclinical safety evaluation.[Methods]Total 80 rats were randomly and evenly divided into high-dose group(1.2 mL/100 g,120 g/kg),middle-dose group(96.0 g/kg),low-dose group(72.0 g/kg)and blank control group.The rats in the treatment groups were administered with corresponding doses of Maxing Erchen Zhike granules,and those in the blank control group were given with equal-amount normal saline.The administration lasted for 30 consecutive days.During the experiment,the rats'feed intake,activity,feces and other conditions and toxicity reactions were observed every day.After 24 h of the last administration,12 rats(half male and half female)were randomly selected from each group.Each of the rats was anesthetized with 10%chloral hydrate solution(0.3 mL/100 g)through intraperitoneal injection and subjected to abdominal aorta blood collection(two tubes)for hematological examination and blood biochemical examination(serum).Then,the main organs of the rats were weighed,and pathological examinations were performed.After that,the main organs were weighed and pathological examination was performed.The remaining rats in each group were discontinued and observed for 14 d.On the 15th d,they were subjected to the same treatment,and the body weight,organ coefficients,hematological indices,blood biochemical indices and pathological indices were examined.[Results]After 30 d of administration,there was no abnormality in the appearance and behavior of the animals.There was no significant difference in the daily consumption of feed among the groups,and there was no special case of weight gain.Among the blood biochemical indices,the ALB and ALT levels of each administration group were significantly different from those of the blank control group(P<0.05).The results of histopathological examination show that there was one case of interstitial pneumonia in each of the high-dose group,middle-dose group and blank control group.After 14 d that the administration was stopped,one case of focal myocarditis appeared in the high-dose group,and one case of interstitial pneumonia appeared in the middle-dose group.[Conclusions]Maxing Erchen Zhike granules are safe to be administered to rats at 100 times the clinical dose.and there should be no safety hazards clinically when used at conventional doses.展开更多
[Objectives]To explore the effects of temperature on the primary and secondary metabolism of Scutellaria baicalensis Georgi during the seed germination.[Methods]The superoxide dismutase(SOD)activity was determined usi...[Objectives]To explore the effects of temperature on the primary and secondary metabolism of Scutellaria baicalensis Georgi during the seed germination.[Methods]The superoxide dismutase(SOD)activity was determined using riboflavin-NBT;peroxidase(POD)activity was determined using guaiacol colorimetric method,catalase(CAT),ascorbate peroxidase(APX),phenylalanine ammonia lyase(PAL)and cinnamic acid-4-hydroxylase(C4H)activity were detected by ultraviolet spectrophotometry,and chalcone synthase(CHS)activity and the content of secondary metabolites were measured by high performance liquid chromatography(HPLC).[Results]The germination rate,germination potential and germination index of S.baicalensis seeds were significantly affected by temperature.The most suitable temperature for the germination of S.baicalensis seeds was 25℃.The activities of SOD,POD and CAT in S.baicalensis seeds treated at low and high temperature were higher than that treated at suitable temperature;the activities of PAL,C4H and CHS of S.baicalensis seeds treated at low and high temperature were lower than that treated at suitable temperature.There was a good positive correlation between flavonoids and soluble sugar,PAL activity and C4H activity,and the correlation coefficients were R=0.894*,R=0.956*and R=0.951*,respectively.[Conclusions]In adverse environment,S.baicalensis seeds have good defense capabilities.During the germination of seeds,the formation of secondary metabolites is significantly correlated to the activity of key enzymes.Therefore,high-quality medicinal materials can be obtained by taking measures to improve the activity of key enzymes.展开更多
In this paper,the resource distribution,pharmacological action and culture status of hirudos were reviewed.Hirudo resources are widely distributed and have a variety of pharmacological effects,but culture is difficult...In this paper,the resource distribution,pharmacological action and culture status of hirudos were reviewed.Hirudo resources are widely distributed and have a variety of pharmacological effects,but culture is difficult,so the scientific development of hirudos culture and solving the problem of hirudos breeding can alleviate the contradiction in the medicine market and contribute to the sustainable development of hirudo medicine.展开更多
While teaching professional knowledge,higher education should organically introduce moral education into classroom teaching,strengthen ideological and political teaching,and realize the goal of moral education.Photovo...While teaching professional knowledge,higher education should organically introduce moral education into classroom teaching,strengthen ideological and political teaching,and realize the goal of moral education.Photovoltaic industry is the only industry in China that ranks first in the world.Putting the concept of“China's PV world first”into the classroom teaching of related disciplines,especially into the teaching of related major courses,has a great promoting effect on improving young students’patriotism and family love,inspiring young students to strive hard for China’s science and technology,inspiring young students’innovative spirit and ability,etc.Taking“China’s PV World No.1”as an example,this paper guides students to establish noble moral character and hard-working spirit from various angles,such as relevant typical cases,relevant data,and relevant foreign reports.It also guides students to establish national pride,carry forward the spirit of self-improvement,build national self-confidence and patriotism,and inspire students to establish the belief of striving for science and technology,and implement it in practical study.展开更多
Higher education is one of the critical education stages,which has an important influence on the student’s future work and life.Science and engineering courses such as solar cells fundamental and applications should ...Higher education is one of the critical education stages,which has an important influence on the student’s future work and life.Science and engineering courses such as solar cells fundamental and applications should not only teach students professional knowledge but also run through ideological and political education elements to improve students’quality in an all-round way.Craftsman’s spiritis especially important for science and engineering students,and it is an important foundation to realizing the“Made in China 2025”strategy.Through typical cases,the craftsman’s spiritis organically integrated into the course design of“Solar Cell Fundamental and Applications”,which is of great significance to the development of our country and society.展开更多
基金Supported by Guangxi Key R&D Plan(GUIKE AB20297010)Guangxi Science and Technology Base and Talent Special Project(GUIKE AD23023011)the National Natural Science Foundation of China(81960889).
文摘[Objectives]Using wild-type AB strain of zebrafish as experimental animal,this study investigated the damaging effect of Wufang Babu Ointment on skin cells,in order to evaluate the skin toxicity of Wufang Babu Ointment.[Methods]Wild-type AB strain of zebrafish with an age of 2 d were taken and fed in different concentrations of Wufang Babu Ointment solution for 24 h.The number of deaths in each group of zebrafish was recorded,and the mortality rate was calculated.Using Origin 8.0 software,the maximum non lethal concentration(MNLC)was simulated.Zebrafish raised in different concentrations of Wufang Babu Ointment solution for 24 h were placed under an anatomical microscope for taking photos,to analyze and calculate the incidence of skin damage in zebrafish.Based on the statistical analysis results of this indicator,the skin toxicity of Wufang Babu Ointment was evaluated.[Results]The MNLC of Wufang Babu Ointment on zebrafish was 671μg/mL;Wufang Babu Ointment can induce skin damage at the concentrations of 224μg/mL(1/3 MNLC)and 671μg/mL(MNLC).[Conclusions]Wufang Babu Ointment had certain skin toxicity to zebrafish.
基金supported by the BJAST High-level Innovation Team Program (No.BGS202001)the Beijing Postdoctoral Research Foundation (No.2022-ZZ-046)+3 种基金the National Natural and Science Foundation of China (No.51972026)the Japan Society for the Promotion of Science (JSPS)Grant-in-Aid for the Scientific Research (KAKENHI,Nos.16H06439 and 20H00297)the Dynamic Alliance for Open Innovations Bridging Human,Environment and Materials,the Cooperative Research Program of“Network Joint Research Center for Materials and Devices.”the scholarship granted to a visiting Ph.D.student of the Inter-University Exchange Project by the China Scholarship Council (CSC,No.201906460113)。
文摘The synthesis of oxygen vacancies(OVs)-modified TiO_(2)under mild conditions is attractive.In this work,OVs were easily introduced in TiO_(2)lattice during the hydrothermal doping process of trivalent iron ions.Theoretical calculations based on a novel charge-compensation structure model were employed with experimental methods to reveal the intrinsic photocatalytic mechanism of Fe-doped TiO_(2)(Fe-TiO_(2)).The OVs formation energy in Fe-TiO_(2)(1.12 eV)was only 23.6%of that in TiO_(2)(4.74 eV),explaining why Fe^(3+)doping could introduce OVs in the TiO_(2)lattice.The calculation results also indicated that impurity states introduced by Fe^(3+)and OVs enhanced the light absorption activity of TiO_(2).Additionally,charge carrier transport was investigated through the carrier lifetime and relative mass.The carrier lifetime of Fe-TiO_(2)(4.00,4.10,and 3.34 ns for 1at%,2at%,and 3at%doping contents,respectively)was longer than that of undoped TiO_(2)(3.22 ns),indicating that Fe^(3+) and OVs could promote charge carrier separation,which can be attributed to the larger relative effective mass of electrons and holes.Herein,Fe-TiO_(2)has higher photocatalytic indoor NO removal activity compared with other photocatalysts because it has strong light absorption activity and high carrier separation efficiency.
基金supported by the Construction Project of Capacity Improvement Plan for Chongqing Municipal Health Commission affiliated unit (2019NLTS001)-ZS03174the operating grant to Chongqing Key Laboratory of Neurodegenerative, and Diseases (Grant No. 1000013)Chongqing Talent Project (Grant No. 2000062)。
文摘Aging, subjected to scientific scrutiny, is extensively defined as a time-dependent decline in functions that involves the majority of organisms. The time-dependent accretion of cellular lesions is generally a universal trigger of aging, while mitochondrial dysfunction is a sign of aging. Dysfunctional mitochondria are identified and removed by mitophagy, a selective form of macroautophagy. Increased mitochondrial damage resulting from reduced biogenesis and clearance may promote the aging process. The primary purpose of this paper is to illustrate in detail the effects of mitophagy on aging and emphasize the associations between mitophagy and other signs of aging, including dietary restriction, telomere shortening, epigenetic alterations, and protein imbalance.The evidence regarding the effects of these elements on aging is still limited. And although the understanding of relationship between mitophagy and aging has been long-awaited, to analyze details of such a relationship remains the main challenge in aging studies.
基金Supported by Guangxi Key Research and Development Project(GK AB2203-5073)Guangxi TCM Appropriate Technology Development and Promotion Project(GZSY22-18)Research Project of Guangxi Administration of Traditional Chinese Medicine(GXZYZ20210209,GXZYA20220180).
文摘[Objectives]The paper was to establish the quality standard of Shenqi Qiangjing granules and to conduct routine inspection of granular dosage forms.[Methods]The particle size,moisture,loss on drying,dissolubility,content uniformity and microbial limit of Shenqi Qiangjing granules were detected.Radix Astragali,Herba Epimedii,Radix Dipsaci and Fructus Schisandrae Chinensis were qualitatively identified by thin layer chromatography(TLC).[Results]The particle size,moisture,loss on drying,dissolubility,content uniformity and microbial limit of Shenqi Qiangjing granules were all in line with the relevant regulations of Pharmacopoeia of the People's Republic of China(2020 edition).Radix Astragali,Herba Epimedii,Radix Dipsaci and Fructus Schisandrae Chinensis had clear fluorescent spots in TLC assay,and no such spots were found in negative control.[Conclusions]The method has the advantages of simple operation,accurate and reliable results,strong specificity and good repeatability,and can effectively control the quality of Shenqi Qiangjing granules.
文摘[Objectives] To identify the species of Hirudo from different sources. [Methods] In accordance with morphological, anatomical and molecular taxonomic characteristics, the identification conclusion was drawn. [Results] The living samples of Hirudo belong to 1 order, 2 families, 3 genera and 5 species, and the dry samples of medicinal leeches belong to 4 species. [Conclusions] The sources of medicinal leeches are complex, and there is a problem that Hirudo from different basic sources are mixed for medication.
基金Supported by Project of Guangxi Clinical Research Center of Traditional Chinese Medicine Encephalopathy(GUIKE AD20238028)High-level Innovation Team and Outstanding Scholar Program of Guangxi Colleges and Universities[GUIJIAORENCAI(2020)6]+1 种基金Academic Team Construction Project of the First Affiliated Hospital of Guangxi University of Traditional Chinese Medicine[YUANZI(2018)146]Key Discipline Construction Project of Guangxi Traditional Chinese Medicine(GZXK-Z-20-13)。
文摘[Objectives]To establish the quality standard of Fuyang Wenshen Granules and carry out the routine examination of granule type.[Methods]The particle size,water content,solubility,loading difference and microbial limit of Fuyang Wenshen Granules were examined,and TLC was used for qualitative identification of Astmgali Radix and Notoginseng Radix Et Rhizoma.[Results]The particle size,water content,solubility,loading difference,and microbial limit of Fuyang Wenshen Granules complied with the relevant provisions of the Pharmacopoeia of the People's Republic of China(2020).The TLC spots of Astmgali Radix and Notoginseng Radix Et Rhizoma were clearly colored,and negative had no interference.[Conclusions]The method was simple,accurate,reliable,specific and reproducible,and can effectively control the quality of Fuyang Wenshen Granules.
基金financially supported by the National Key R&D Program of China(2017YFA0204700)the Joint Research Funds of Department of Science&Technology of Shaanxi Province and Northwestern Polytechnical University(2020GXLH-Z-021)+1 种基金the China-Sweden Joint Mobility Project(51811530018)the Fundamental Research Funds for the Central Universities.
文摘Convenient,rapid,and accurate detection of cardiac troponin I(cTnI)is crucial in early diagnosis of acute myocardial infarction(AMI).A paper-based electrochemical immunosensor is a promising choice in this field,because of the flexibility,porosity,and cost-efficacy of the paper.However,paper is poor in electronic conductivity and surface functionality.Herein,we report a paper-based electrochemical immunosensor for the label-free detection of cTnI with the working electrode modified by MXene(Ti_(3)C_(2))nanosheets.In order to immobilize the bio-receptor(anti-cTnI)on the MXene-modified working electrode,the MXene nanosheets were functionalized by aminosilane,and the functionalized MXene was immobilized onto the surface of the working electrode through Nafion.The large surface area of the MXene nanosheets facilitates the immobilization of antibodies,and the excellent conductivity facilitates the electron transfer between the electrochemical species and the underlying electrode surface.As a result,the paper-based immunosensor could detect cTnI within a wide range of 5-100 ng/mL with a detection limit of 0.58 ng/mL.The immunosensor also shows outstanding selectivity and good repeatability.Our MXene-modified paper-based electrochemical immunosensor enables fast and sensitive detection of cTnI,which may be used in real-time and cost-efficient monitoring of AMI diseases in clinics.
基金supported by the Youth Scientific and Technological Innovation Team Foundation of Southwest Petroleum University(2019CXTD09)the Program of Introducing Talents of Discipline to Chinese Universities(111 Plan)(D18016).
文摘Coalbed methane(CBM)drilling and completion technologies(DCTs)are signifcant basis for achieving efcient CBM exploration and exploitation.Characteristics of CBM reservoirs vary in diferent regions around the world,thereby,it is crucial to develop,select and apply the optimum DCTs for each diferent CBM reservoir.This paper frstly reviews the development history of CBM DCTs throughout worldwide and clarifes its overall development tendency.Secondly,diferent well types and its characteristics of CBM exploitation are summarized,and main application scopes of these well types are also discussed.Then,the key technologies of CBM drilling(directional drilling tools,measurement while drilling,geo-steering drilling,magnetic guidance drilling,underbalanced drilling and drilling fuids),and the key technologies of CBM completion(open-hole,cavity and under-ream completion,cased-hole completion,screen pipe completion and horizontal well completion)are summarized and analyzed,it is found that safe,economic and efcient development of CBM is inseparable from the support of advanced technologies.Finally,based on the current status of CBM development,the achievements,existing challenges and future prospects are summarized and discussed from the perspective of CBM DCTs.
文摘Bone-like nanohydroxyapatite powders (b-nanoHA) were synthesized in simulated body fluid (SBF). The b-nanoHA, gelatin (Gel) and Polyvinyl Alcohol (PVA) were used to prepare bone-like composites (b-nanoHA/ Gel/PVA) at room temperature. Characterizations of b-nanoHA powders and b-nanoHA/Gel/PVA composites were investigated by using X-ray diffraction (XRD), transmission electron microscopy (TEM), High-resolution transmission electron microscopy (HRTEM), scanning electron microscopy (SEM) and Fourier transform infrared spectroscopy (FT-IR). Bending strength and compressive strength of the composite were tested. It was found that microstructure of the b-nanoHA powders was whisker shape and its crystalline degree was low similar to natural bone, bending strength and compressive strength of the b-nanoHA/Gel/PVA composite depended on the mixing ratio of HA, Gel and PVA, and also PVA could induce the network formation in the b-nanoHA/Gel/ PVA composite.
基金Supported by Science and Technology Development Plan of Traditional Chinese Medicine in Shandong Province(2019-2020)(2019-1036)Public Health Service Subsidy for TCM Department(Cai She[2019]39).
文摘This article reviews the species,identification,chemical composition and pharmacological effects of hirudo.Hirudo is widely sourced,difficult to identify,contains a variety of active ingredients,and has a variety of pharmacological effects.In-depth research on hirudo is of great significance to the quality control and clinical application of hirudo.
基金supported by the Teaching and Research Project on“Ideological and Political Special Item”(No.2020szzx04)Project(No.2019zd01)of Qilu University of Technologypartly supported by the Natural Science Foundation of Shandong Province(No.ZR2020MF104).
文摘At present,the education of specialized courses in science and engineering still focuses on imparting specialized knowledge,which seriously lacks the embodiment of educating students.Taking the specialized course“Solid Luminescent Materials”as an example,the new mode of ideological/political teaching is integrated into the specialized course,giving full play to the role of the main position of the specialized class,so as to realize the aim of teaching specialized knowledge in cooperation with educating students and imperceptibly influencing the ideological/political teaching.In this paper,the design of ideological/political knowledge points and the integration of ideological/political cases are carried out from the aspects of teaching concepts,teaching contents and teaching cases.By adhering to the cooperation between moral and financial classroom and specialized education,the educational function of university specialized courses can be effectively brought into play,which is expected to guide students to enhance their awareness of energy conservation,environmental protection,innovation and patriotism.
基金Supported by Special Fund for Public Health Service of Traditional Chinese Medicine Departments([2017]66)Traditional Chinese Medicine Science and Technology Development Project(2017-522)
文摘[Objectives] This study was conducted to clarify the roles of macronutrients in regulating plant primary and secondary metabolism of Scutellaria baicalensis Georgi. [Methods] The contents of chlorophyll(Chl) and soluble sugar were detected with ultraviolet spectrophotometry. The activities of phenylalanine ammonia-lyase(PAL), cinnamate 4-hydroxylase(C_4H) and chalcone synthase(CHS) were determined with ultraviolet spectrophotometry. Secondary metabolites were detected by high performance liquid chromatography(HPLC). [Results] The content of chlorophyll in treatments N, P and K was increased, showing significant difference from that of the control(P<0.05). Among them, Treatment N has the highest content of chlorophyll. Soluble sugar content in treatments N, P and K(K>N>P>CK) was increased considerably and had significant difference from that of the control(P<0.05). Compared with the control, PAL and C_4H activity in treatments N, P and K were increased dramatically by 186.57%, 134.09%, 306.91% and 73.21%, 28.91%, 247.57%, respectively. CHS activity was also increased sharply in treatments N, P and K. Scutellarin, baicalin, wogonoside, baicalein, wogonin and oroxylin A contents in all treatments N, P and K(K>N>P) were increased, and had significant difference from that of the control(P<0.05). [Conclusions] The results showed that macronutrients can increase leaf chlorophyll content, soluble sugar content, PAL activity, C_4H activity and secondary metabolites of S. baicalensis.
基金Supported by 2017 Traditional Chinese Medicine Public Health Service Specific Grant(CS[2017]66)Shandong Traditional Chinese Medicine Science and Technology Development Program(2017-522)
文摘[Objectives]This study was conducted to study the Germination characteristics and secondary metabolism regulation of Scutellaria baicalensis Georgi under osmotic stress.[Methods]The activity of praline,soluble sugar and protein was detected with ultraviolet spectrophotometry.The activity of phenylalanine ammonia-lyase(PAL)and cinnamic acid 4-hydroxylase(C_4H)was determined with ultraviolet spectrophotometry.The secondary metabolites were detected by high performance liquid chromatography(HPLC).[Results]The germination percentage,germination potentiality and germination index decreased at first and increased subsequently along with the enhancement of PEG concentration.The praline content,the soluble sugar content and the soluble protein content in S.baicalensis seedlings increased along with the enhancement of PEG concentration and the differences from the CK were significant(P<0.05).The PAL and C_4H in S.baicalensis seedlings decreased after osmotic stress and the differences from the CK were significant(P<0.05).The results indicate that,the scutellarin,baicalin,wogonoside,baicalein,wogonin and oroxylinA contents decreased after osmotic stress and the differences from the CK were significant(P<0.05).[Conclusions]The quality of the skullcaps could be improved by improving the activity of PAL and C_4H.
基金the Training Project of National Traditional Chinese Medicine Characteristic Technology Inheritance Talents(T20184828005,201548160100).
文摘[Objectives]The content of total anthraquinone and 5 free anthraquinones in different processed products of rhubarb were determined.[Methods]Using emodin as the reference,the concentration of 1%magnesium acetate methanol solution was used for color development,and the absorbance was measured at 516 nm wavelength;the mobile phase was methanol-0.1%phosphoric acid(76∶24)aqueous solution,the detection wavelength was set at 256 nm,the flow rate was 1.0 mL/min,the column temperature was 30℃,and the sample size was 10μL.[Results]The content of total anthraquinones in rhubarb was determined by UV spectrophotometry.The linear range was 0.98-23.52μg/mL,R2=0.9993,the average recoveries were 100.12%,99.66%and 99.15%,with RSDs of 1.58%,0.94%and 1.41%,respectively.The linear ranges of emodin,emodin,emodin,chrysophanol and emodin methyl ether were 0.0207-0.414(R2=0.9999),0.1721-3.442(R2=0.9996),0.0203-0.406(R2=0.9999),0.148-2.96(R2=0.9998)and 0.164-3.28(R2=0.9993),respectively.[Conclusions]The contents of total anthraquinones and free anthraquinones in different processed products of rhubarb were determined by UV spectrophotometry and HPLC.The method can be used for quality control of rhubarb and its different processed products.
基金Scientific Research Project of the First Affiliated Hospital of Guangxi University of Chinese Medicine(2017ZJ006)Key Research and Development Project of Department of Science and Technology of Guangxi Zhuang Autonomous Region(AB19110003).
文摘[Objectives]To study the long-term toxicity of Maxing Erchen Zhike granules to rats after intragastric administration,so as to provide reference for its preclinical safety evaluation.[Methods]Total 80 rats were randomly and evenly divided into high-dose group(1.2 mL/100 g,120 g/kg),middle-dose group(96.0 g/kg),low-dose group(72.0 g/kg)and blank control group.The rats in the treatment groups were administered with corresponding doses of Maxing Erchen Zhike granules,and those in the blank control group were given with equal-amount normal saline.The administration lasted for 30 consecutive days.During the experiment,the rats'feed intake,activity,feces and other conditions and toxicity reactions were observed every day.After 24 h of the last administration,12 rats(half male and half female)were randomly selected from each group.Each of the rats was anesthetized with 10%chloral hydrate solution(0.3 mL/100 g)through intraperitoneal injection and subjected to abdominal aorta blood collection(two tubes)for hematological examination and blood biochemical examination(serum).Then,the main organs of the rats were weighed,and pathological examinations were performed.After that,the main organs were weighed and pathological examination was performed.The remaining rats in each group were discontinued and observed for 14 d.On the 15th d,they were subjected to the same treatment,and the body weight,organ coefficients,hematological indices,blood biochemical indices and pathological indices were examined.[Results]After 30 d of administration,there was no abnormality in the appearance and behavior of the animals.There was no significant difference in the daily consumption of feed among the groups,and there was no special case of weight gain.Among the blood biochemical indices,the ALB and ALT levels of each administration group were significantly different from those of the blank control group(P<0.05).The results of histopathological examination show that there was one case of interstitial pneumonia in each of the high-dose group,middle-dose group and blank control group.After 14 d that the administration was stopped,one case of focal myocarditis appeared in the high-dose group,and one case of interstitial pneumonia appeared in the middle-dose group.[Conclusions]Maxing Erchen Zhike granules are safe to be administered to rats at 100 times the clinical dose.and there should be no safety hazards clinically when used at conventional doses.
基金Shandong Province Traditional Chinese Medicine Science and Technology Development Plan Project in 2019-2020(2019-1036)2019 Chinese Medicine Public Health Service Subsidy Project:National Chinese Medicine Resources Survey Project(Cai She[2019]No.39).
文摘[Objectives]To explore the effects of temperature on the primary and secondary metabolism of Scutellaria baicalensis Georgi during the seed germination.[Methods]The superoxide dismutase(SOD)activity was determined using riboflavin-NBT;peroxidase(POD)activity was determined using guaiacol colorimetric method,catalase(CAT),ascorbate peroxidase(APX),phenylalanine ammonia lyase(PAL)and cinnamic acid-4-hydroxylase(C4H)activity were detected by ultraviolet spectrophotometry,and chalcone synthase(CHS)activity and the content of secondary metabolites were measured by high performance liquid chromatography(HPLC).[Results]The germination rate,germination potential and germination index of S.baicalensis seeds were significantly affected by temperature.The most suitable temperature for the germination of S.baicalensis seeds was 25℃.The activities of SOD,POD and CAT in S.baicalensis seeds treated at low and high temperature were higher than that treated at suitable temperature;the activities of PAL,C4H and CHS of S.baicalensis seeds treated at low and high temperature were lower than that treated at suitable temperature.There was a good positive correlation between flavonoids and soluble sugar,PAL activity and C4H activity,and the correlation coefficients were R=0.894*,R=0.956*and R=0.951*,respectively.[Conclusions]In adverse environment,S.baicalensis seeds have good defense capabilities.During the germination of seeds,the formation of secondary metabolites is significantly correlated to the activity of key enzymes.Therefore,high-quality medicinal materials can be obtained by taking measures to improve the activity of key enzymes.
文摘In this paper,the resource distribution,pharmacological action and culture status of hirudos were reviewed.Hirudo resources are widely distributed and have a variety of pharmacological effects,but culture is difficult,so the scientific development of hirudos culture and solving the problem of hirudos breeding can alleviate the contradiction in the medicine market and contribute to the sustainable development of hirudo medicine.
基金supported by the Teaching and Research Project on“Ideological and Political Special Item”(No.2020szzx04)Project(No.2019zd01)of Qilu University of Technology,and Research Project of Education and Teaching Reform in Shandong University(No.2021Y289)Natural Science Foundation of Shandong Province(No.ZR ZR2020MF104).
文摘While teaching professional knowledge,higher education should organically introduce moral education into classroom teaching,strengthen ideological and political teaching,and realize the goal of moral education.Photovoltaic industry is the only industry in China that ranks first in the world.Putting the concept of“China's PV world first”into the classroom teaching of related disciplines,especially into the teaching of related major courses,has a great promoting effect on improving young students’patriotism and family love,inspiring young students to strive hard for China’s science and technology,inspiring young students’innovative spirit and ability,etc.Taking“China’s PV World No.1”as an example,this paper guides students to establish noble moral character and hard-working spirit from various angles,such as relevant typical cases,relevant data,and relevant foreign reports.It also guides students to establish national pride,carry forward the spirit of self-improvement,build national self-confidence and patriotism,and inspire students to establish the belief of striving for science and technology,and implement it in practical study.
基金This work is supported by the Teaching and Research Project on“Ideological and Political Special Item”(No.2020szzx04)and Project(No.2019zd01)of Qilu University of Technologyand Research Project of Education and Teaching Reform in Shandong University(No.2021Y289).
文摘Higher education is one of the critical education stages,which has an important influence on the student’s future work and life.Science and engineering courses such as solar cells fundamental and applications should not only teach students professional knowledge but also run through ideological and political education elements to improve students’quality in an all-round way.Craftsman’s spiritis especially important for science and engineering students,and it is an important foundation to realizing the“Made in China 2025”strategy.Through typical cases,the craftsman’s spiritis organically integrated into the course design of“Solar Cell Fundamental and Applications”,which is of great significance to the development of our country and society.
