Walnut dreg protein hydrolysates(WDPHs)exhibit a variety of biological activities,however,the cyclooxygenase-2(COX-2)inhibitory peptide of WDPHs remain unclear.The aim of this study was to rapidly screen for such pept...Walnut dreg protein hydrolysates(WDPHs)exhibit a variety of biological activities,however,the cyclooxygenase-2(COX-2)inhibitory peptide of WDPHs remain unclear.The aim of this study was to rapidly screen for such peptides in WDPHs through a combination of in silico and in vitro analysis.In total,1262 peptide sequences were observed by nano liquid chromatography/tandem mass spectrometry(nano LC-MS/MS)and 4 novel COX-2 inhibitory peptides(AGFP,FPGA,LFPD,and VGFP)were identified.Enzyme kinetic data indicated that AGFP,FPGA,and LFPD displayed mixed-type COX-2 inhibition,whereas VGFP was a non-competitive inhibitor.This is mainly because the peptides form hydrogen bonds and hydrophobic interactions with residues in the COX-2 active site.These results demonstrate that computer analysis combined with in vitro evaluation allows for rapid screening of COX-2 inhibitory peptides in walnut protein dregs.展开更多
Halide perovskites with naturally coupled electron-ion dynamics hold greatpotential for nonvolatile memory applications. Self-rectifying memristors arepromising as they can avoid sneak currents and simplify device con...Halide perovskites with naturally coupled electron-ion dynamics hold greatpotential for nonvolatile memory applications. Self-rectifying memristors arepromising as they can avoid sneak currents and simplify device configuration.Here we report a self-rectifying memristor firstly achieved in a single perovskite(NH CINH_(3))_(3)PbI_(5) (abbreviated as (IFA)_(3)PbI_(5)), which is sandwiched byAg and ITO electrodes as the simplest cell in a crossbar array device configuration.The iodide ions of (IFA)_(3)PbI_(5) can be easily activated, of which the migrationin the bulk contributes to the resistance hysteresis and the reaction withAg at the interface contributes to the spontaneous formation of AgI. The perfectcombination of n-type AgI and p-type (IFA)_(3)PbI_(5) gives rise to the rectificationfunction like a p–n diode. Such a self-rectifying memristor exhibits therecord-low set power consumption and voltage. This work emphasizes that themultifunction of ions in perovskites can simplify the fabrication procedure,decrease the programming power, and increase the integration density offuture memory devices.展开更多
Diabetes mellitus(DM)is a chronic metabolic disease characterized by hyperglycemia,which poses a serious threat to human health.Moringa oleifera Lam.is a medicinal and edible plant with various physiological functions...Diabetes mellitus(DM)is a chronic metabolic disease characterized by hyperglycemia,which poses a serious threat to human health.Moringa oleifera Lam.is a medicinal and edible plant with various physiological functions.However,its main hypoglycemic components and mechanisms are still unclear.In this study,network pharmacology and bioinformatics were used to analyze the potential bioactive substances of M.oleifera leaf extract(MOLE)and its hypoglycemic mechanism.Studies have shown that MOLE has the effect of increasing glucose consumption in insulin resistant-HepG2 cells.MOLE was found to contain 975 compounds by ultrahigh performance liquid chromatography-mass spectrometry(UHPLC-MS).Network pharmacology analysis indicated that the main active component was robinetin and the identified core genes were AKT1 and GAPDH.KEGG pathway enrichment analysis showed that the hypoglycemic effect of MOLE may be closely related to the PI3K-Akt signaling pathway.This study revealed the possible active components and mechanisms of action of M.oleifera for hypoglycemia,laying the theoretical foundation for subsequent studies.展开更多
基金supported by the Major Project of Science and Technology Department of Yunnan Province (202002AA100005 and 202102AE090027-2)the Project of Yunnan Province Food and Drug Homologous Resources Functional Food Innovation Team (A3032023057)+2 种基金the YEFICRC project of Yunnan provincial key programs (2019ZG009)Yunnan Province Ten Thousand Plan Industrial Technology Talents project (YNWR-CYJS-2020-010)the Yunnan Provincial Department of Science and Technology Agricultural Joint Special Project (202101BD070001-120)。
文摘Walnut dreg protein hydrolysates(WDPHs)exhibit a variety of biological activities,however,the cyclooxygenase-2(COX-2)inhibitory peptide of WDPHs remain unclear.The aim of this study was to rapidly screen for such peptides in WDPHs through a combination of in silico and in vitro analysis.In total,1262 peptide sequences were observed by nano liquid chromatography/tandem mass spectrometry(nano LC-MS/MS)and 4 novel COX-2 inhibitory peptides(AGFP,FPGA,LFPD,and VGFP)were identified.Enzyme kinetic data indicated that AGFP,FPGA,and LFPD displayed mixed-type COX-2 inhibition,whereas VGFP was a non-competitive inhibitor.This is mainly because the peptides form hydrogen bonds and hydrophobic interactions with residues in the COX-2 active site.These results demonstrate that computer analysis combined with in vitro evaluation allows for rapid screening of COX-2 inhibitory peptides in walnut protein dregs.
基金National Key Research and Development Program of China,Grant/Award Numbers:2020YFA0308900,2022YFB3602801National Natural Science Foundation of China,Grant/Award Numbers:52373290,62288102。
文摘Halide perovskites with naturally coupled electron-ion dynamics hold greatpotential for nonvolatile memory applications. Self-rectifying memristors arepromising as they can avoid sneak currents and simplify device configuration.Here we report a self-rectifying memristor firstly achieved in a single perovskite(NH CINH_(3))_(3)PbI_(5) (abbreviated as (IFA)_(3)PbI_(5)), which is sandwiched byAg and ITO electrodes as the simplest cell in a crossbar array device configuration.The iodide ions of (IFA)_(3)PbI_(5) can be easily activated, of which the migrationin the bulk contributes to the resistance hysteresis and the reaction withAg at the interface contributes to the spontaneous formation of AgI. The perfectcombination of n-type AgI and p-type (IFA)_(3)PbI_(5) gives rise to the rectificationfunction like a p–n diode. Such a self-rectifying memristor exhibits therecord-low set power consumption and voltage. This work emphasizes that themultifunction of ions in perovskites can simplify the fabrication procedure,decrease the programming power, and increase the integration density offuture memory devices.
基金supported by the Major Project of Science and Technology Department of Yunnan Province(202002AA100005 and 202102AE090027-2)Cassava Industrial Technology System of China(CARS-11-YNTY)Yunnan Province Ten Thousand Plan Industrial Technology Talents project(YNWR-CYJS-2020-010).
文摘Diabetes mellitus(DM)is a chronic metabolic disease characterized by hyperglycemia,which poses a serious threat to human health.Moringa oleifera Lam.is a medicinal and edible plant with various physiological functions.However,its main hypoglycemic components and mechanisms are still unclear.In this study,network pharmacology and bioinformatics were used to analyze the potential bioactive substances of M.oleifera leaf extract(MOLE)and its hypoglycemic mechanism.Studies have shown that MOLE has the effect of increasing glucose consumption in insulin resistant-HepG2 cells.MOLE was found to contain 975 compounds by ultrahigh performance liquid chromatography-mass spectrometry(UHPLC-MS).Network pharmacology analysis indicated that the main active component was robinetin and the identified core genes were AKT1 and GAPDH.KEGG pathway enrichment analysis showed that the hypoglycemic effect of MOLE may be closely related to the PI3K-Akt signaling pathway.This study revealed the possible active components and mechanisms of action of M.oleifera for hypoglycemia,laying the theoretical foundation for subsequent studies.
