The back propagation(BP)neural network method is widely used in bathymetry based on multispectral satellite imagery.However,the classical BP neural network method faces a potential problem because it easily falls into...The back propagation(BP)neural network method is widely used in bathymetry based on multispectral satellite imagery.However,the classical BP neural network method faces a potential problem because it easily falls into a local minimum,leading to model training failure.This study confirmed that the local minimum problem of the BP neural network method exists in the bathymetry field and cannot be ignored.Furthermore,to solve the local minimum problem of the BP neural network method,a bathymetry method based on a BP neural network and ensemble learning(BPEL)is proposed.First,the remote sensing imagery and training sample were used as input datasets,and the BP method was used as the base learner to produce multiple water depth inversion results.Then,a new ensemble strategy,namely the minimum outlying degree method,was proposed and used to integrate the water depth inversion results.Finally,an ensemble bathymetric map was acquired.Anda Reef,northeastern Jiuzhang Atoll,and Pingtan coastal zone were selected as test cases to validate the proposed method.Compared with the BP neural network method,the root-mean-square error and the average relative error of the BPEL method can reduce by 0.65–2.84 m and 16%–46%in the three test cases at most.The results showed that the proposed BPEL method could solve the local minimum problem of the BP neural network method and obtain highly robust and accurate bathymetric maps.展开更多
Pericytes are the main cellular components of tiny arteries and capillaries.Studies have found that pericytes can undergo morphological contraction or relaxation under stimulation by cytokines,thus affecting the contr...Pericytes are the main cellular components of tiny arteries and capillaries.Studies have found that pericytes can undergo morphological contraction or relaxation under stimulation by cytokines,thus affecting the contraction and relaxation of microvessels and playing an essential role in regulating vascular microcirculation.Moreover,due to the characteristics of stem cells,pericytes can differentiate into a variety of inflammatory cell phenotypes,which then affect the immune function.Additionally,pericytes can also participate in angiogenesis and wound healing by interacting with endothelial cells in vascular microcirculation disorders.Here we review the origin,biological phenotype and function of pericytes,and discuss the potential mechanisms of pericytes in vascular microcirculation disorders,especially in pulmonary hypertension,so as to provide a sound basis and direction for the prevention and treatment of vascular microcirculation diseases.展开更多
Background:Aberrant expression of microRNAs(miRNAs)has been associated with the pathogenesis of pulmonary hypertension(PH).It is,however,not clear whether miRNAs are involved in estrogen rescue of PH.Methods:Fresh pla...Background:Aberrant expression of microRNAs(miRNAs)has been associated with the pathogenesis of pulmonary hypertension(PH).It is,however,not clear whether miRNAs are involved in estrogen rescue of PH.Methods:Fresh plasma samples were prepared from 12 idiopathic pulmonary arterial hypertension(IPAH)patients and 12 healthy controls undergoing right heart cath-eterization in Shanghai Pulmonary Hospital.From each sample,5μg of total RNA was tagged and hybridized on microRNA microarray chips.Monocrotaline-induced PH(MCT-PH)male rats were treated with 17β-estradiol(E_(2))or vehicle.Subgroups were cotreated with estrogen receptor(ER)antagonist or with antagonist of miRNA.Results:Many circulating miRNAs,including miR-21-5p and miR-574-5p,were mark-edly expressed in patients and of interest in predicting mean pulmonary arterial pres-sure elevation in patients.The expression of miR-21-5p in the lungs was significantly upregulated in MCT-PH rats compared with the controls.However,miR-574-5p showed no difference in the lungs of MCT-PH rats and controls.miR-21-5p was se-lected for further analysis in rats as E_(2) strongly regulated it.E_(2) decreased miR-21-5p expression in the lungs of MCT-PH rats by ERβ.E_(2) reversed miR-21-5p target gene FilGAP downregulation in the lungs of MCT-PH rats.The abnormal expression of RhoA,ROCK2,Rac1 and c-Jun in the lungs of MCT-PH rats was inhibited by E_(2) and miR-21-5p antagonist.Conclusions:miR-21-5p level was remarkably associated with PH severity in patients.Moreover,the miR-21-5p/FilGAP signaling pathway modulated the protective effect of E_(2) on MCT-PH through ERβ.展开更多
The Convolutional Neural Network(CNN)is a widely used deep neural network.Compared with the shallow neural network,the CNN network has better performance and faster computing in some image recognition tasks.It can eff...The Convolutional Neural Network(CNN)is a widely used deep neural network.Compared with the shallow neural network,the CNN network has better performance and faster computing in some image recognition tasks.It can effectively avoid the problem that network training falls into local extremes.At present,CNN has been applied in many different fields,including fault diagnosis,and it has improved the level and efficiency of fault diagnosis.In this paper,a two-streams convolutional neural network(TCNN)model is proposed.Based on the short-time Fourier transform(STFT)spectral and Mel Frequency Cepstrum Coefficient(MFCC)input characteristics of two-streams acoustic emission(AE)signals,an AE signal processing and classification system is constructed and compared with the traditional recognition methods of AE signals and traditional CNN networks.The experimental results illustrate the effectiveness of the proposed model.Compared with single-stream convolutional neural network and a simple Long Short-Term Memory(LSTM)network,the performance of TCNN which combines spatial and temporal features is greatly improved,and the accuracy rate can reach 100%on the current database,which is 12%higher than that of single-stream neural network.展开更多
The Pt-Sb system was critically assessed by means of CALPHAD technique. Based on the experimental data in the literature, the excess Gibbs energies of the solution phases (liquid, rhombohedral, fcc) were modeled with ...The Pt-Sb system was critically assessed by means of CALPHAD technique. Based on the experimental data in the literature, the excess Gibbs energies of the solution phases (liquid, rhombohedral, fcc) were modeled with the Redlich-Kister equation. The five intermetallic compounds, Pt7Sb, Pt3Sb, Pt3Sb2, PtSb, and PtSb2 were treated as stochiometric compounds and expressed as the formula (Pt)m(Sb)n. The intermetallic compound, Pt5Sb with a homogenerity ranges 0.155 -?0.189 Sb, were treated as the formula (Pt,Sb)m(Pt,Sb)n. A set of self-consistent thermodynamic parameters of the Pt-Sb system was obtained.展开更多
Background:Our previous study found that mouse embryonic neural stem cell(NSC)-derived exosomes(EXOs)regulated NSC differentiation via the miR-9/Hes1 axis.However,the effects of EXOs on brain microvascular endothelial...Background:Our previous study found that mouse embryonic neural stem cell(NSC)-derived exosomes(EXOs)regulated NSC differentiation via the miR-9/Hes1 axis.However,the effects of EXOs on brain microvascular endothelial cell(BMEC)dysfunction via the miR-9/Hes1 axis remain unknown.Therefore,the current study aimed to determine the effects of EXOs on BMEC proliferation,migration,and death via the miR-9/Hes1 axis.Methods:Immunofluorescence,quantitative real-time polymerase chain reaction,cell counting kit-8 assay,wound healing assay,calcein-acetoxymethyl/propidium iodide staining,and hematoxylin and eosin staining were used to determine the role and mechanism of EXOs on BMECs.Results:EXOs promoted BMEC proliferation and migration and reduced cell death under hypoxic conditions.The overexpression of miR-9 promoted BMEC prolifera-tion and migration and reduced cell death under hypoxic conditions.Moreover,miR-9 downregulation inhibited BMEC proliferation and migration and also promoted cell death.Hes1 silencing ameliorated the effect of amtagomiR-9 on BMEC proliferation and migration and cell death.Hyperemic structures were observed in the regions of the hippocampus and cortex in hypoxia-induced mice.Meanwhile,EXO treatment improved cerebrovascular alterations.Conclusion:NSC-derived EXOs can promote BMEC proliferation and migra-tion and reduce cell death via the miR-9/Hes1 axis under hypoxic conditions.Therefore,EXO therapeutic strategies could be considered for hypoxia-induced vascular injury.展开更多
Background:Schistosomiasis remains a major public health concern in China and an epidemiological survey has revealed that schistosome-infected bovines and goats are the main transmission sources for the disease.Theref...Background:Schistosomiasis remains a major public health concern in China and an epidemiological survey has revealed that schistosome-infected bovines and goats are the main transmission sources for the disease.Therefore,development of a sensitive technique for the diagnosis of schistosomiasis in domestic animals is necessary.Method:A novel colloidal gold immunochromatography assay(GICA)strip was developed for detecting Schistosoma japonicum in domestic animals.The colloidal gold was conjugated with recombinant streptococcal protein G(rSPG).As the test and control lines,the schistosome soluble egg antigen and rSPG,respectively,were blotted on nitrocellulose membrane.Results:The lowest detectable serum dilution was 1∶640 for schistosome-infected buffaloes.The cross-reaction rate of GICA was 14.29%with Paramphistomum sp.in buffaloes,16.67%with Haemonchus sp.in goats,and 33.33%with Orientobilharzia sp.in goats.These results were slightly lower and similar to those obtained through ELISA.Moreover,the strips for detecting S.japonicum in mice,rabbits,buffaloes,and goats showed high sensitivity(100.00%,100.00%,100.00%,and 100.00%,respectively)and specificity(100.00%,100.00%,94.23%,and 88.64%,respectively).And the sensitivity or specificity of the GICA strips did not present any significant differences after storage for 12 months at room temperature.When compared with ELISA,the GICA strips exhibited similar sensitivity and specificity in the diagnosis of schistosomiasis in mice,rabbits,buffaloes,and goats.Besides,only 5μl of serum are required for the test and the detection can be completed within 5 min.Conclusion:This study is the first to develop a GICA strip using gold-rSPG conjugate for the diagnosing of schistosomiasis in domestic animals,and preliminary results showed that the developed strip may be suitable for large-scale screening of schistosomiasis in endemic areas.展开更多
Liquid biopsy has become an emerging technology in the detection of cancer related biomarkers as well as the continuous monitoring of cancer treatment.There has been extensive research on the applications of magnetic ...Liquid biopsy has become an emerging technology in the detection of cancer related biomarkers as well as the continuous monitoring of cancer treatment.There has been extensive research on the applications of magnetic nanotechnologies in liquid biopsies from the separation of target analytes to the detection of cancer biomarkers.Magnetic separation plays an important role in increasing both the efficiency and sensitivity of the liquid biopsy process.The detection of cancer biomarkers through magnetic nanosensors also possesses many advantages such as low background noise,high sensitivity,short assay time,and the ability to detect multiple biomarkers at the same time.This review focuses on the recent advances of magnetic nanotechnologies in liquid biopsies for cancer detection and its future potential in comparison with other technologies.展开更多
基金The National Natural Science Foundation of China under contract No.42001401the China Postdoctoral Science Foundation under contract No.2020M671431+1 种基金the Fundamental Research Funds for the Central Universities under contract No.0209-14380096the Guangxi Innovative Development Grand Grant under contract No.2018AA13005.
文摘The back propagation(BP)neural network method is widely used in bathymetry based on multispectral satellite imagery.However,the classical BP neural network method faces a potential problem because it easily falls into a local minimum,leading to model training failure.This study confirmed that the local minimum problem of the BP neural network method exists in the bathymetry field and cannot be ignored.Furthermore,to solve the local minimum problem of the BP neural network method,a bathymetry method based on a BP neural network and ensemble learning(BPEL)is proposed.First,the remote sensing imagery and training sample were used as input datasets,and the BP method was used as the base learner to produce multiple water depth inversion results.Then,a new ensemble strategy,namely the minimum outlying degree method,was proposed and used to integrate the water depth inversion results.Finally,an ensemble bathymetric map was acquired.Anda Reef,northeastern Jiuzhang Atoll,and Pingtan coastal zone were selected as test cases to validate the proposed method.Compared with the BP neural network method,the root-mean-square error and the average relative error of the BPEL method can reduce by 0.65–2.84 m and 16%–46%in the three test cases at most.The results showed that the proposed BPEL method could solve the local minimum problem of the BP neural network method and obtain highly robust and accurate bathymetric maps.
基金Program of Fundamental Research Funds for the Central Universities,Grant/Award Number:22120220562Program of Natural Science Foundation of Shanghai,Grant/Award Number:201409004100 and 21ZR1453800+1 种基金Three Year Action Plan to Promote Clinical Skills and Clinical Innovation in Municipal Hospitals,Grant/Award Number:SHDC2020CR6016-002 and SHDC2020CR4021Program of Shanghai Pulmonary Hospital,Grant/Award Number:fkzr2320 and FKLY20005。
文摘Pericytes are the main cellular components of tiny arteries and capillaries.Studies have found that pericytes can undergo morphological contraction or relaxation under stimulation by cytokines,thus affecting the contraction and relaxation of microvessels and playing an essential role in regulating vascular microcirculation.Moreover,due to the characteristics of stem cells,pericytes can differentiate into a variety of inflammatory cell phenotypes,which then affect the immune function.Additionally,pericytes can also participate in angiogenesis and wound healing by interacting with endothelial cells in vascular microcirculation disorders.Here we review the origin,biological phenotype and function of pericytes,and discuss the potential mechanisms of pericytes in vascular microcirculation disorders,especially in pulmonary hypertension,so as to provide a sound basis and direction for the prevention and treatment of vascular microcirculation diseases.
基金National Natural Science Foundation of China,Grant/Award Number:8 1870042Natural Science Foundation of Shanghai,Grant/Award Number:21ZR1453800。
文摘Background:Aberrant expression of microRNAs(miRNAs)has been associated with the pathogenesis of pulmonary hypertension(PH).It is,however,not clear whether miRNAs are involved in estrogen rescue of PH.Methods:Fresh plasma samples were prepared from 12 idiopathic pulmonary arterial hypertension(IPAH)patients and 12 healthy controls undergoing right heart cath-eterization in Shanghai Pulmonary Hospital.From each sample,5μg of total RNA was tagged and hybridized on microRNA microarray chips.Monocrotaline-induced PH(MCT-PH)male rats were treated with 17β-estradiol(E_(2))or vehicle.Subgroups were cotreated with estrogen receptor(ER)antagonist or with antagonist of miRNA.Results:Many circulating miRNAs,including miR-21-5p and miR-574-5p,were mark-edly expressed in patients and of interest in predicting mean pulmonary arterial pres-sure elevation in patients.The expression of miR-21-5p in the lungs was significantly upregulated in MCT-PH rats compared with the controls.However,miR-574-5p showed no difference in the lungs of MCT-PH rats and controls.miR-21-5p was se-lected for further analysis in rats as E_(2) strongly regulated it.E_(2) decreased miR-21-5p expression in the lungs of MCT-PH rats by ERβ.E_(2) reversed miR-21-5p target gene FilGAP downregulation in the lungs of MCT-PH rats.The abnormal expression of RhoA,ROCK2,Rac1 and c-Jun in the lungs of MCT-PH rats was inhibited by E_(2) and miR-21-5p antagonist.Conclusions:miR-21-5p level was remarkably associated with PH severity in patients.Moreover,the miR-21-5p/FilGAP signaling pathway modulated the protective effect of E_(2) on MCT-PH through ERβ.
基金This research was funded by the National Natural Science Foundation of China[Nos.51908285,61673108 and 61571106]School-level Research Fund Project of Nanjing Institute of Technology[YKJ201975]China Postdoctoral Science Foundation[2018M630559].
文摘The Convolutional Neural Network(CNN)is a widely used deep neural network.Compared with the shallow neural network,the CNN network has better performance and faster computing in some image recognition tasks.It can effectively avoid the problem that network training falls into local extremes.At present,CNN has been applied in many different fields,including fault diagnosis,and it has improved the level and efficiency of fault diagnosis.In this paper,a two-streams convolutional neural network(TCNN)model is proposed.Based on the short-time Fourier transform(STFT)spectral and Mel Frequency Cepstrum Coefficient(MFCC)input characteristics of two-streams acoustic emission(AE)signals,an AE signal processing and classification system is constructed and compared with the traditional recognition methods of AE signals and traditional CNN networks.The experimental results illustrate the effectiveness of the proposed model.Compared with single-stream convolutional neural network and a simple Long Short-Term Memory(LSTM)network,the performance of TCNN which combines spatial and temporal features is greatly improved,and the accuracy rate can reach 100%on the current database,which is 12%higher than that of single-stream neural network.
文摘The Pt-Sb system was critically assessed by means of CALPHAD technique. Based on the experimental data in the literature, the excess Gibbs energies of the solution phases (liquid, rhombohedral, fcc) were modeled with the Redlich-Kister equation. The five intermetallic compounds, Pt7Sb, Pt3Sb, Pt3Sb2, PtSb, and PtSb2 were treated as stochiometric compounds and expressed as the formula (Pt)m(Sb)n. The intermetallic compound, Pt5Sb with a homogenerity ranges 0.155 -?0.189 Sb, were treated as the formula (Pt,Sb)m(Pt,Sb)n. A set of self-consistent thermodynamic parameters of the Pt-Sb system was obtained.
基金Program of Natural Science Foundation of Shanghai,Grant/Award Number:21ZR1453800 and 22ZR1452400Program of National Natural Science Foundation of China,Grant/Award Number:82370057+3 种基金Fundamental Research Funds for the Central Universities,Grant/Award Number:22120220562Program of Shanghai Municipal Health Commission,Grant/Award Number:20204Y0384Program of National Key Research and Development Project of China,Grant/Award Number:2023YFC2509500。
文摘Background:Our previous study found that mouse embryonic neural stem cell(NSC)-derived exosomes(EXOs)regulated NSC differentiation via the miR-9/Hes1 axis.However,the effects of EXOs on brain microvascular endothelial cell(BMEC)dysfunction via the miR-9/Hes1 axis remain unknown.Therefore,the current study aimed to determine the effects of EXOs on BMEC proliferation,migration,and death via the miR-9/Hes1 axis.Methods:Immunofluorescence,quantitative real-time polymerase chain reaction,cell counting kit-8 assay,wound healing assay,calcein-acetoxymethyl/propidium iodide staining,and hematoxylin and eosin staining were used to determine the role and mechanism of EXOs on BMECs.Results:EXOs promoted BMEC proliferation and migration and reduced cell death under hypoxic conditions.The overexpression of miR-9 promoted BMEC prolifera-tion and migration and reduced cell death under hypoxic conditions.Moreover,miR-9 downregulation inhibited BMEC proliferation and migration and also promoted cell death.Hes1 silencing ameliorated the effect of amtagomiR-9 on BMEC proliferation and migration and cell death.Hyperemic structures were observed in the regions of the hippocampus and cortex in hypoxia-induced mice.Meanwhile,EXO treatment improved cerebrovascular alterations.Conclusion:NSC-derived EXOs can promote BMEC proliferation and migra-tion and reduce cell death via the miR-9/Hes1 axis under hypoxic conditions.Therefore,EXO therapeutic strategies could be considered for hypoxia-induced vascular injury.
基金This study was financially supported by the Basic Foundation for Scientific Research of State-level Public Welfare Institutes of China(Grant no.2014JB01).
文摘Background:Schistosomiasis remains a major public health concern in China and an epidemiological survey has revealed that schistosome-infected bovines and goats are the main transmission sources for the disease.Therefore,development of a sensitive technique for the diagnosis of schistosomiasis in domestic animals is necessary.Method:A novel colloidal gold immunochromatography assay(GICA)strip was developed for detecting Schistosoma japonicum in domestic animals.The colloidal gold was conjugated with recombinant streptococcal protein G(rSPG).As the test and control lines,the schistosome soluble egg antigen and rSPG,respectively,were blotted on nitrocellulose membrane.Results:The lowest detectable serum dilution was 1∶640 for schistosome-infected buffaloes.The cross-reaction rate of GICA was 14.29%with Paramphistomum sp.in buffaloes,16.67%with Haemonchus sp.in goats,and 33.33%with Orientobilharzia sp.in goats.These results were slightly lower and similar to those obtained through ELISA.Moreover,the strips for detecting S.japonicum in mice,rabbits,buffaloes,and goats showed high sensitivity(100.00%,100.00%,100.00%,and 100.00%,respectively)and specificity(100.00%,100.00%,94.23%,and 88.64%,respectively).And the sensitivity or specificity of the GICA strips did not present any significant differences after storage for 12 months at room temperature.When compared with ELISA,the GICA strips exhibited similar sensitivity and specificity in the diagnosis of schistosomiasis in mice,rabbits,buffaloes,and goats.Besides,only 5μl of serum are required for the test and the detection can be completed within 5 min.Conclusion:This study is the first to develop a GICA strip using gold-rSPG conjugate for the diagnosing of schistosomiasis in domestic animals,and preliminary results showed that the developed strip may be suitable for large-scale screening of schistosomiasis in endemic areas.
基金supported by the National Key Research and Development Program of China(2020YFA080370 and 2020YFA0803702)the Frontiers Science Center for New Organic Matter,Nankai University(63181206)+1 种基金the National Natural Science Foundation of China(21875116,81921004,51973096,and 31970716)the financial support from TinGo Regenerative Medicine(Tianjin)Co.,Ltd.
基金the Institute of Engineering in Medicine of the University of Minnesota through FY18 IEM Seed Grant Funding Program and the Doctoral Dissertation Fellowship.
文摘Liquid biopsy has become an emerging technology in the detection of cancer related biomarkers as well as the continuous monitoring of cancer treatment.There has been extensive research on the applications of magnetic nanotechnologies in liquid biopsies from the separation of target analytes to the detection of cancer biomarkers.Magnetic separation plays an important role in increasing both the efficiency and sensitivity of the liquid biopsy process.The detection of cancer biomarkers through magnetic nanosensors also possesses many advantages such as low background noise,high sensitivity,short assay time,and the ability to detect multiple biomarkers at the same time.This review focuses on the recent advances of magnetic nanotechnologies in liquid biopsies for cancer detection and its future potential in comparison with other technologies.
