Objective: To analyze the correlation between salivary cortisol content and anxiety and depression in pregnant and postpartum women. Method: 300 pregnant and postpartum women who were admitted from January 2021 to Dec...Objective: To analyze the correlation between salivary cortisol content and anxiety and depression in pregnant and postpartum women. Method: 300 pregnant and postpartum women who were admitted from January 2021 to December 2021 were selected as the research subjects. They were all tested with the Hamilton Anxiety Scale (HAMA) and the Edinburgh Postnatal Depression Scale (EPDS). 40 pregnant women with anxiety and depression were selected as the observation group, and 50 normal pregnant women were selected as the control group, adopting logistic regression analysis to investigate the correlation between salivary cortisol and postpartum anxiety and depression during pregnancy. Result: The salivary cortisol levels in the observation group were significantly higher than those in the control group before bedtime, after waking up the next day, 15 minutes after waking up, 30 minutes after waking up, 60 minutes after waking up, and 1 hour before lunch (p < 0.05). Maternal depression and anxiety were not related to age, weight, gestational age, negative events, or history of adverse pregnancy and childbirth (p > 0.05). Maternal depression and anxiety are closely related to salivary cortisol levels and educational background (p Conclusion: There is a close correlation between anxiety and depression and an increase in cortisol levels, suggesting that the salivary cortisol awakening response during pregnancy has a predictive effect on the occurrence of postpartum depression.展开更多
Stimuli-responsive materials hold great potential for the development of smart materials due to their specifically tailored characteristics.However,it is challenging to understand internal molecular dynamics when the ...Stimuli-responsive materials hold great potential for the development of smart materials due to their specifically tailored characteristics.However,it is challenging to understand internal molecular dynamics when the macroscopic mechanics of materials change in response to specific applied stimuli.Herein,we present the biological composite fibers of which mechanical properties can be reversibly controlled on demand by photothermal effect of an alternating near-infrared light irradiation.In stark contrast to the weakening of the mechanical properties of conventional materials by heating,the mechanical performance of the obtained fibers are significantly enhanced,showing an increase of Young’s modulus by a factor of four.The outstanding photothermal-mechanical behavior relies on the evolution of hydrogen bonds within the system.We envision that this type of fiber material will inspire a new strategy for the construction of smart devices.展开更多
Cellular senescence provides a protective barrier against tumorigenesis in precancerous or normal tissues upon distinct stressors.However,the detailed mechanisms by which tumor cells evade premature senescence to mali...Cellular senescence provides a protective barrier against tumorigenesis in precancerous or normal tissues upon distinct stressors.However,the detailed mechanisms by which tumor cells evade premature senescence to malignant progression remain largely elusive.Here we reported that RBM4 adversely impacted cellular senescence to favor glutamine-dependent survival of esophageal squamous cell carcinoma(ESCC)cells by dictating the activity of LKB1,a critical governor of cancer metabolism.The level of RBM4 was specifically elevated in ESCC compared to normal tissues,and RBM4 overexpression promoted the malignant phenotype.RBM4 contributed to overcome H-RAS-or doxorubicin-induced senescence,while its depletion caused P27-dependent senescence and proliferation arrest by activating LKB1-AMPK-mTOR cascade.Mechanistically,RBM4 competitively bound LKB1 to disrupt the LKB1/STRAD/MO25 heterotrimeric complex,subsequently recruiting the E3 ligase TRIM26 to LKB1,promoting LKB1 ubiquitination and degradation in nucleus.Therefore,such molecular process leads to bypassing senescence and sustaining cell proliferation through the activation of glutamine metabolism.Clinically,the ESCC patients with high RBM4 and low LKB1 have significantly worse overall survival than those with low RBM4 and high LKB1.The RBM4 high/LKB1 low expression confers increased sensitivity of ESCC cells to glutaminase inhibitor CB-839,providing a novel insight into mechanisms underlying the glutamine-dependency to improve the efficacy of glutamine inhibitors in ESCC therapeutics.展开更多
Most plasmalemmal proteins are organized into clusters to modulate various cellular functions.However,the machineries that regulate protein clustering remain largely unclear.Here,with EGFR as an example,we directly an...Most plasmalemmal proteins are organized into clusters to modulate various cellular functions.However,the machineries that regulate protein clustering remain largely unclear.Here,with EGFR as an example,we directly and in detail visualized the entire process of EGFR from synthesis to secretion onto the plasma membrane(PM)using a high-speed,high-resolution spinning-disk confocal microscope.First,colocalization imaging revealed that EGFR secretory vesicles underwent transport from the ER to the Golgi to the PM,eventually forming different distribution forms on the apical and basal membranes;that is,most EGFR formed larger clusters on the apical membrane than the basal membrane.A dynamic tracking image and further siRNA interference experiment confirmed that fusion of secretory vesicles with the plasma membrane led to EGFR clusters,and we showed that EGFR PM clustering may be intimately related to EGFR signaling and cell proliferation.Finally,we found that the size and origin of the secretory vesicles themselves may determine the difference in the distribution patterns of EGFR on the PM.More importantly,we showed that actin influenced the EGFR distribution by controlling the fusion of secretory vesicles with the PM.Collectively,a comprehensive understanding of the EGFR secretion process helps us to unravel the EGFR clustering process and elucidate the key factors determining the differences in the spatial distribution of EGFR PM,highlighting the correlation between EGFR secretion and its PM distribution pattern.展开更多
Garnet amphibolite in the Baoyintu Group,Inner Mongolia, is mainly composed of garnet, hornblende,plagioclase, quartz and minor rutile/ilmenite. Garnet occurs as porphyroblasts surrounded by plagioclase, forming typic...Garnet amphibolite in the Baoyintu Group,Inner Mongolia, is mainly composed of garnet, hornblende,plagioclase, quartz and minor rutile/ilmenite. Garnet occurs as porphyroblasts surrounded by plagioclase, forming typical ‘‘white-eye socket'' texture. Garnet shows grossular content of 0.26–0.28 and pyrope of 0.11–0.13, without significant zoning. Plagioclase is generally zoned with anorthite(An) increasing from core to rim. The P–T pseudosection calculated using THERMOCALC in the system Mn NCKFMASHTO shows that the garnet amphibolite experienced a clockwise P–T path with a peak at*1.3 GPa/725 °C defined from the minimum An content in plagioclase and maximum pyrope content in garnet,followed by an isothermal decompression. LA-ICP-MS zircon dating for the garnet amphibolite indicates metamorphic ages of 399 ± 6 Ma. The peak P–T condition corresponds to a thermal gradient of *18 °C/km, indicating typical medium-pressure type that commonly occurs in orogenic process with crustal thickening. We prefer to interpret this orogenic event to be a result of collision,following the closure of the Paleo-Asian Ocean before theearly Devonian although there is a popular view that the Paleo-Asian Ocean may have lasted to the early Mesozoic.展开更多
Alternative splicing is a critical process to generate protein diversity.However,whether and how alternative splicing regulates autophagy remains largely elusive.Here we systematically identify the splicing factor SRS...Alternative splicing is a critical process to generate protein diversity.However,whether and how alternative splicing regulates autophagy remains largely elusive.Here we systematically identify the splicing factor SRSF1 as an autophagy suppressor.Specifically,SRSF1 inhibits autophagosome formation by reducing the accumulation of LC3-ⅡI and numbers of autophagosomes in different cell lines.Mechanistically,SRSF1 promotes the splicing of the long isoform of Bcl-x that interacts with Beclinl,thereby dissociating the Beclin1-PIK3C3 complex.In addition,SRSF1 also directly interacts with PIK3C3 to disrupt the interaction between Beclinl and PIK3C3.Consequently,the decrease of SRSF1 stabilizes the Beclinl and PIK3C3 complex and activates autophagy.Interestingly,SRSF1 can be degraded by starvation-and oxidative stresses-induced autophagy through interacting with LC3-Ⅱ,whereas reduced SRSF1 further promotes autophagy.This positive feedback is critical to inhibiting Gefitinib-resistant cancer cell progression both in vitro and in vivo.Consistently,the expression level of SRSF1 is inversely correlated to LC3 level in clinical cancer samples.Our study not only provides mechanistic insights of alternative splicing in autophagy regulation but also discovers a new regulatory role of SRSF1 in tumorigenesis,thereby offering a novel avenue for potential cancer therapeutics.展开更多
文摘Objective: To analyze the correlation between salivary cortisol content and anxiety and depression in pregnant and postpartum women. Method: 300 pregnant and postpartum women who were admitted from January 2021 to December 2021 were selected as the research subjects. They were all tested with the Hamilton Anxiety Scale (HAMA) and the Edinburgh Postnatal Depression Scale (EPDS). 40 pregnant women with anxiety and depression were selected as the observation group, and 50 normal pregnant women were selected as the control group, adopting logistic regression analysis to investigate the correlation between salivary cortisol and postpartum anxiety and depression during pregnancy. Result: The salivary cortisol levels in the observation group were significantly higher than those in the control group before bedtime, after waking up the next day, 15 minutes after waking up, 30 minutes after waking up, 60 minutes after waking up, and 1 hour before lunch (p < 0.05). Maternal depression and anxiety were not related to age, weight, gestational age, negative events, or history of adverse pregnancy and childbirth (p > 0.05). Maternal depression and anxiety are closely related to salivary cortisol levels and educational background (p Conclusion: There is a close correlation between anxiety and depression and an increase in cortisol levels, suggesting that the salivary cortisol awakening response during pregnancy has a predictive effect on the occurrence of postpartum depression.
基金supported by the National Natural Science Foundation of China(grant nos.22125701,21877104,21834007,21878258,22020102003)the National Key R&D Program of China(grant nos.2021YFB3502300,2021YFF0701800,2020YFA0908900)Grants from Natural Science Foundation of Guangdong Province,China(grant nos.2021A1515010253,202102020523).
文摘Stimuli-responsive materials hold great potential for the development of smart materials due to their specifically tailored characteristics.However,it is challenging to understand internal molecular dynamics when the macroscopic mechanics of materials change in response to specific applied stimuli.Herein,we present the biological composite fibers of which mechanical properties can be reversibly controlled on demand by photothermal effect of an alternating near-infrared light irradiation.In stark contrast to the weakening of the mechanical properties of conventional materials by heating,the mechanical performance of the obtained fibers are significantly enhanced,showing an increase of Young’s modulus by a factor of four.The outstanding photothermal-mechanical behavior relies on the evolution of hydrogen bonds within the system.We envision that this type of fiber material will inspire a new strategy for the construction of smart devices.
基金supported by the National Natural Science Foundation of China (82225034,81830088 to Y.W.,82103148 to Y.Q.81872247 to W.Z.)+4 种基金the Department of Science and Technology of Liaoning Province (2021JH6/10500160 to Y.W.)the Basic Scientific Research Project of Education Department of Liaoning Province (LJKQZ2021104 to Y.Q.)the Science and Technology Innovation Talent Support Program of Dalian (2022RQ056 Y.Q.)the Science and Technology Innovation Foundation of Dalian (2022JJ11CG009 to Y.W.)Dalian High Level Talents Renovation Supporting Program (2019RQ097 to W.Z.).
文摘Cellular senescence provides a protective barrier against tumorigenesis in precancerous or normal tissues upon distinct stressors.However,the detailed mechanisms by which tumor cells evade premature senescence to malignant progression remain largely elusive.Here we reported that RBM4 adversely impacted cellular senescence to favor glutamine-dependent survival of esophageal squamous cell carcinoma(ESCC)cells by dictating the activity of LKB1,a critical governor of cancer metabolism.The level of RBM4 was specifically elevated in ESCC compared to normal tissues,and RBM4 overexpression promoted the malignant phenotype.RBM4 contributed to overcome H-RAS-or doxorubicin-induced senescence,while its depletion caused P27-dependent senescence and proliferation arrest by activating LKB1-AMPK-mTOR cascade.Mechanistically,RBM4 competitively bound LKB1 to disrupt the LKB1/STRAD/MO25 heterotrimeric complex,subsequently recruiting the E3 ligase TRIM26 to LKB1,promoting LKB1 ubiquitination and degradation in nucleus.Therefore,such molecular process leads to bypassing senescence and sustaining cell proliferation through the activation of glutamine metabolism.Clinically,the ESCC patients with high RBM4 and low LKB1 have significantly worse overall survival than those with low RBM4 and high LKB1.The RBM4 high/LKB1 low expression confers increased sensitivity of ESCC cells to glutaminase inhibitor CB-839,providing a novel insight into mechanisms underlying the glutamine-dependency to improve the efficacy of glutamine inhibitors in ESCC therapeutics.
基金supported by the National Natural Science Foundation of China(nos.22150003,21727816 and 21721003)the Scientific Instrument Developing Project of the Chinese Academy of Sciences(ZDKYYQ20220005)the Foundation of Science and Technology Department of Jilin Province(YDZJ202201ZYTS530).
文摘Most plasmalemmal proteins are organized into clusters to modulate various cellular functions.However,the machineries that regulate protein clustering remain largely unclear.Here,with EGFR as an example,we directly and in detail visualized the entire process of EGFR from synthesis to secretion onto the plasma membrane(PM)using a high-speed,high-resolution spinning-disk confocal microscope.First,colocalization imaging revealed that EGFR secretory vesicles underwent transport from the ER to the Golgi to the PM,eventually forming different distribution forms on the apical and basal membranes;that is,most EGFR formed larger clusters on the apical membrane than the basal membrane.A dynamic tracking image and further siRNA interference experiment confirmed that fusion of secretory vesicles with the plasma membrane led to EGFR clusters,and we showed that EGFR PM clustering may be intimately related to EGFR signaling and cell proliferation.Finally,we found that the size and origin of the secretory vesicles themselves may determine the difference in the distribution patterns of EGFR on the PM.More importantly,we showed that actin influenced the EGFR distribution by controlling the fusion of secretory vesicles with the PM.Collectively,a comprehensive understanding of the EGFR secretion process helps us to unravel the EGFR clustering process and elucidate the key factors determining the differences in the spatial distribution of EGFR PM,highlighting the correlation between EGFR secretion and its PM distribution pattern.
基金supported by the National Basic Research Program of China(‘‘973’’Program)(2013CB429801)the China Survey of Geology(1212011121077)
文摘Garnet amphibolite in the Baoyintu Group,Inner Mongolia, is mainly composed of garnet, hornblende,plagioclase, quartz and minor rutile/ilmenite. Garnet occurs as porphyroblasts surrounded by plagioclase, forming typical ‘‘white-eye socket'' texture. Garnet shows grossular content of 0.26–0.28 and pyrope of 0.11–0.13, without significant zoning. Plagioclase is generally zoned with anorthite(An) increasing from core to rim. The P–T pseudosection calculated using THERMOCALC in the system Mn NCKFMASHTO shows that the garnet amphibolite experienced a clockwise P–T path with a peak at*1.3 GPa/725 °C defined from the minimum An content in plagioclase and maximum pyrope content in garnet,followed by an isothermal decompression. LA-ICP-MS zircon dating for the garnet amphibolite indicates metamorphic ages of 399 ± 6 Ma. The peak P–T condition corresponds to a thermal gradient of *18 °C/km, indicating typical medium-pressure type that commonly occurs in orogenic process with crustal thickening. We prefer to interpret this orogenic event to be a result of collision,following the closure of the Paleo-Asian Ocean before theearly Devonian although there is a popular view that the Paleo-Asian Ocean may have lasted to the early Mesozoic.
基金supported by the National Key Research and Development Program of China (2016YFC1102601 and 2017YFC1104901)the National Natural Science Foundation of China (51571190 and 51631007)the Key Research Program of Frontier Sciences of Chinese Academy of Sciences (QYZDJ-SSW-JSC031)
基金This work was supported by the National Natural Science Foundation of China(81830088,81422038,91540110,and 31471235 to Y.W.,81872247 and 31400726 to W.Z.)the Department of Education of Liaoning Province(the"Liaoning Supports High Level Talents Innovation and Entrepreneurship Program"XLYC1802067 to Y.W.)+1 种基金the Department of Science and Technology of Dalian City(the HDalian Supports High Level Talents Innovation and Entrepreneurship Program" 2016RJ02 to Y.W.)the Newton Advanced Fellowship from the Academy of Medical Sciences in UK(JXR11831 to Y.W.).
文摘Alternative splicing is a critical process to generate protein diversity.However,whether and how alternative splicing regulates autophagy remains largely elusive.Here we systematically identify the splicing factor SRSF1 as an autophagy suppressor.Specifically,SRSF1 inhibits autophagosome formation by reducing the accumulation of LC3-ⅡI and numbers of autophagosomes in different cell lines.Mechanistically,SRSF1 promotes the splicing of the long isoform of Bcl-x that interacts with Beclinl,thereby dissociating the Beclin1-PIK3C3 complex.In addition,SRSF1 also directly interacts with PIK3C3 to disrupt the interaction between Beclinl and PIK3C3.Consequently,the decrease of SRSF1 stabilizes the Beclinl and PIK3C3 complex and activates autophagy.Interestingly,SRSF1 can be degraded by starvation-and oxidative stresses-induced autophagy through interacting with LC3-Ⅱ,whereas reduced SRSF1 further promotes autophagy.This positive feedback is critical to inhibiting Gefitinib-resistant cancer cell progression both in vitro and in vivo.Consistently,the expression level of SRSF1 is inversely correlated to LC3 level in clinical cancer samples.Our study not only provides mechanistic insights of alternative splicing in autophagy regulation but also discovers a new regulatory role of SRSF1 in tumorigenesis,thereby offering a novel avenue for potential cancer therapeutics.
