期刊文献+
共找到2篇文章
< 1 >
每页显示 20 50 100
微小RNA-29a-3p调节卷曲蛋白4表达影响高脂血症大鼠种植体骨整合的实验研究 被引量:5
1
作者 刘飞 王志峰 +3 位作者 刘芳芳 徐巾诏 刘奇博 蓝菁 《华西口腔医学杂志》 CAS CSCD 北大核心 2019年第2期200-207,共8页
目的研究microRNA-29a-3p(miR-29a-3p)对高脂环境下大鼠骨髓间充质干细胞(BMSCs)成骨分化和高脂血症大鼠种植体骨整合的影响及其作用位点。方法 1)体外实验:对BMSCs分别进行普通和高脂成骨诱导,通过逆转录实时定量聚合酶链反应和Western... 目的研究microRNA-29a-3p(miR-29a-3p)对高脂环境下大鼠骨髓间充质干细胞(BMSCs)成骨分化和高脂血症大鼠种植体骨整合的影响及其作用位点。方法 1)体外实验:对BMSCs分别进行普通和高脂成骨诱导,通过逆转录实时定量聚合酶链反应和Western blot检测miR-29a-3p及成骨相关因子碱性磷酸酶(ALP)、Runt相关基因2(Runx2)的基因和蛋白质表达;高脂培养的BMSCs分别转染miR-29a-3p模拟物、抑制物及阴性对照(NC)质粒,RT-qPCR检测miR-29a-3p、ALP及Runx2基因表达情况,Western blot检测ALP、Runx2蛋白表达情况。通过靶基因预测软件(Target Scan、MiRNA.org等)预测miR-29a-3p与成骨相关的靶基因为卷曲蛋白4(Fzd4),双荧光素酶报告检测miR-29a-3p与Fzd4的相互作用关系。2)体内实验:高脂血症大鼠为实验组,普通大鼠为对照组,两组分别植入种植体,检测种植体周围骨组织中miR-29a-3p、ALP、Runx2的表达差异;行种植体-骨组织的硬组织切片,亚甲基蓝-酸性品红染色,进行组织学观察。分别将miR-29a-3p过表达慢病毒载体及空白对照慢病毒载体注射入高脂血症大鼠,种植体植入后3、10 d检测种植体周围骨组织中ALP、Runx2的表达变化以研究miR-29a-3p对高脂血症大鼠成骨的作用。结果高脂组与普通组相比,ALP、Runx2和miR-29a-3p表达下调,BMSCs成骨分化能力下降。miR-29a-3p模拟物组与抑制物组相比,ALP、Runx2表达升高,BMSCs成骨分化能力增强。体内实验也得到了相似结果。双荧光素酶报告基因分析证实miR29a-3p通过直接结合3’-UTR抑制Fzd4表达。结论miR-29a-3p对高脂环境下大鼠BMSCs成骨分化起正向调节作用,可直接与Fzd4结合调节成骨分化;mi R-29a-3p能够促进高脂血症大鼠种植体周围成骨标志基因的表达,有利于骨整合。 展开更多
关键词 微小RNA-29a-3p 高脂血症 卷曲蛋白4 双荧光素酶报告基因 骨整合
下载PDF
Safety assessment of monosodium glutamate based on intestinal function and flora in mice
2
作者 jinzhao xu Mengqi Tang +2 位作者 Yini Liu Jinghan xu Xiaoxi xu 《Food Science and Human Wellness》 SCIE 2022年第1期155-164,共10页
Although monosodium glutamate(MSG)is a widely used food additive,its safety and systemic side effects have not been fully clarified.The intestinal flora is closely associated with human health;however,it remains uncle... Although monosodium glutamate(MSG)is a widely used food additive,its safety and systemic side effects have not been fully clarified.The intestinal flora is closely associated with human health;however,it remains unclear whether MSG consumption can affect health by acting on the intestinal flora.In this study,serum biomarkers,intestinal structure,intestinal immunity,and intestinal flora were examined to investigate the effects of different doses of sodium glutamate on the body,intestinal function,and intestinal flora of mice.The results showed that 30 mg/kg MSG had no significant effect on serum C-reactive protein,trimethylamine N-oxide,angiotensin II,intestinal interleukin(IL)-1β,IL-6,tumor necrosis factor-α,secretory IgA and fecal albumin in mice,but also promoted intestinal development and regulated the intestinal flora.Moreover,1500 mg/kg MSG increased the risk of cardiovascular disease and damaged the intestinal structure and flora.In this study,MSG was also found to be healthier than salt at the equivalent sodium concentration.Collectively,these findings suggested that low doses of MSG were safe for mice and may have some health benefits as a probiotic by promoting intestinal development and regulating the intestinal flora. 展开更多
关键词 Monosodium glutamate Safety assessment Intestinal flora Intestinal inflammation
下载PDF
上一页 1 下一页 到第
使用帮助 返回顶部