Objective:To explore the detection and clinical significance of peripheral blood CTC,CA125,CA153,and CEA levels in patients with DCIS.Methods:210 patients who received surgical treatment for breast cancer in our hospi...Objective:To explore the detection and clinical significance of peripheral blood CTC,CA125,CA153,and CEA levels in patients with DCIS.Methods:210 patients who received surgical treatment for breast cancer in our hospital from January 2019 to December 2022 were selected as the research subjects.According to the postoperative pathology,100 cases were divided into breast cancer and 110 benign breast tumor groups.One hundred ten healthy patients undergoing physical examination during the same period were selected as the control group.CA153,CA125,and CEA levels were observed in the three groups.Results:The levels of CA153,CA125,and CEA in the breast cancer group were significantly higher than those in the benign breast tumor group and the control group(P<0.05);the levels of CA153,CA125,and CEA in the benign breast tumor group were all higher than those in the control group,but the differences were not statistically significant;among the three tumor markers,CA153 has the highest sensitivity at 39.00%,CA125 has the second highest sensitivity at 18.00%,and CEA has the lowest sensitivity at 17.00%;The sensitivity of the two joint tests of CA153+CA125,CA153+CEA,and CA125+CEA for the diagnosis of breast cancer were 50.00%,48.00%,and 26.00%respectively;the sensitivity of the three joint tests is the highest,reaching 53.00%,while the specificity of the joint tests was lower than the individual tests.Conclusion:Detection of peripheral blood CTC,CA125,CA153,and CEA levels has specific reference significance for the treatment and prognosis of DCIS patients.展开更多
Objective:To explore the value of circulating free(cfDNA)content in the clinical diagnosis and treatment of triple-negative breast cancer(TNBC).Methods:A total of 39 TNBC patients,45 non-TNBC patients,and 50 healthy i...Objective:To explore the value of circulating free(cfDNA)content in the clinical diagnosis and treatment of triple-negative breast cancer(TNBC).Methods:A total of 39 TNBC patients,45 non-TNBC patients,and 50 healthy individuals admitted to the Baoding First Central Hospital during 2019-2022 were recruited.The clinical data,peripheral blood cfDNA concentration,and clinicopathological indicators of the patients were observed and analyzed.Results:The difference in clinical indicators such as age,age range,tumor size,clinical stage,and lymph node metastasis between patients with TNBC and non-TNBC was insignificant(P>0.05).The cfDNA concentrations(ng/mL)of the TNBC group,non-TNBC group,and healthy group were 24.12±4.98,15.36±4.12,and 3.12±1.02,respectively,and they are statistically different(P<0.05).The difference in cfDNA concentration was insignificant between TNBC patients with tumors≤2 cm and>2 cm(P>0.05)but was significant between TNBC patients with clinical stages I+II and III+IV(P<0.05).The cfDNA concentration in TNBC patients with lymph node metastasis was significantly higher than those without lymph node metastasis(P<0.05).Conclusion:cfDNA has an important application value in the diagnosis and treatment of breast cancer.By detecting the cfDNA level and its gene variation,valuable information about the progress and treatment effects of breast cancer can be obtained.This non-invasive detection method has a wide range of applications and can be used for early screening,auxiliary diagnosis,efficacy evaluation,and recurrence monitoring of breast cancer.展开更多
The endoplasmic reticulum plays an extremely important role in the process of cellular protein secretion.The cyclic AMP-responsive element-binding protein 3(CREB3)transcription factor family is closely associated with...The endoplasmic reticulum plays an extremely important role in the process of cellular protein secretion.The cyclic AMP-responsive element-binding protein 3(CREB3)transcription factor family is closely associated with the secretion and transport of proteins within the endoplasmic reticulum.As a member of the CREB3 transcription factor family,cyclic AMP-responsive element-binding protein 3-like protein 2(CREB3L2)stands out as a non-classical sensor within the endoplasmic reticulum.CREB3L2 can detect and regulate endoplasmic reticulum pressure,exert control over the processes of protein transport and secretion,participate in the development of tumor cells,and is also closely linked to the development of certain human tissues and organs.This article aims to review the role of CREB3L2 in tissue development and disease,shedding light on the related mechanisms of CREB3L2 in cancer development.The goal is to provide insights and directions for further analysis of CREB3L2.展开更多
Pathology is the gold standard for clinical diagnosis and an important bridge between clinical work and basic disciplines.Therefore,training qualified clinical pathologists is very important for clinical work.It is ch...Pathology is the gold standard for clinical diagnosis and an important bridge between clinical work and basic disciplines.Therefore,training qualified clinical pathologists is very important for clinical work.It is challenging to diagnose lymphohematopoietic diseases in practice since they made up a large percentage of resident doctors’standardized training and there are numerous knowledge nodes associated with them.Therefore,this training base adopts the“case-introduction”teaching mode to help resident doctors master the knowledge nodes required by the outline in the study of lymphohematopoietic system pathology.展开更多
In many countries,colorectal cancer is one of the common cancers.It has been confirmed that microsatellite instability(MSI)is one of the most important molecular changes in colorectal cancer.As the recognized precurso...In many countries,colorectal cancer is one of the common cancers.It has been confirmed that microsatellite instability(MSI)is one of the most important molecular changes in colorectal cancer.As the recognized precursor lesions of colorectal cancer,adenomas and some polyps also have MSI phenomenon.This article analyzes the MSI of colorectal cancer and its closely related adenomas and polyps in domestic and foreign literature,and further discusses how to identify colorectal cancer at an early stage and the impact of MSI on the prognosis and treatment of colorectal cancer.展开更多
Circulating free DNA(cfDNA)refers to the free DNA nucleotides in body fluids,but the dynamic response in vivo tumor load and full assessment of genetic heterogeneity in cancer patients provides a non-invasive diagnost...Circulating free DNA(cfDNA)refers to the free DNA nucleotides in body fluids,but the dynamic response in vivo tumor load and full assessment of genetic heterogeneity in cancer patients provides a non-invasive diagnostic approach.It is one of the effective biomarkers for cancer screening and diagnosis,and also provides for the prognosis for patients with advanced cancer and forecast information.In this paper,the clinical application of cfDNA is reviewed.展开更多
Objective:To compare the effects of clinical application of chemiluminescence and real-time,fluorescence-based quantitative PCR in the detection Epstein-Barr virus(EBV).Methods:The data of chemiluminescence and real-t...Objective:To compare the effects of clinical application of chemiluminescence and real-time,fluorescence-based quantitative PCR in the detection Epstein-Barr virus(EBV).Methods:The data of chemiluminescence and real-time fluorescent quantitative PCR.fromipaEsfwo were suspected of being infectea w1tn rito1 roro January 2016 to January 2019 in our hospital were analyzed.The specific stage of EBV infection was analyzed,and the differences in results of the two detection methods were compared.Results:Chemiluminescence method was used to detect EBV infection during the active phase.The sensitivity of the chemiluminescence method was 76.7%(56/73)and the real-time quantitative PCRmethod was 90.4%(66/73).There was a statistical difference between the two detection methods(P<0.05).Conclusion:There was no statistical difference in positive predictive values between the chemiluminescence method and the real-time,fluorescence-based quantitative PCR method in the detection of EBV infection,but the sensitivity of chemiluminescence method is slightly lower than the real-time quantitative PCRmethod.It is noteworthy that chemiluminescence method is convenient and fast while the real-time,fluorescence-based quantitative PCR method is more accurate,which can provide a more accurate reference for clinical treatment.展开更多
Objective:To explore the expression of human myxovirus resistance protein A(MxA)in triple negative breast cancer(TNBC)and its relationship with prognosis.Methods:144 cases of TNBC confirmed by pathology before or afte...Objective:To explore the expression of human myxovirus resistance protein A(MxA)in triple negative breast cancer(TNBC)and its relationship with prognosis.Methods:144 cases of TNBC confirmed by pathology before or after surgery from January 2014 to January 2017 in the First Central Hospital of Baoding City were retrospectively collected.Western blotting was used to detect the expression of MxA protein in TNBC and adjacent breast tissues.According to the expression of MxA protein,144 TNBC patients were divided into low MxA protein expression group(n=91)and MxA protein high expression group(n=53)for subsequent comparison of prognosis of patients in between these two groups.Results:The expression of MxA protein in TNBC tissue was lower than that of adjacent breast tissue,and the difference was statistically significant(P<0.05).The patients in high MxA expression group had higher loco-regional recurrence-free rate,disease-free survival(DFS)rate,and overall survival(OS)rate than those in low MxA expression group for 3 years.On the other hand,the distant metastasis rate was lower in the high expression group compared to that in the low MxA expression group,and the difference was statistically significant(P<0.05).Conclusion:In triple-negative breast cancer,high MxA expression is a potential predictor of TNBC prognosis.展开更多
Objective:To study the expression and significance of cyclinD2 and Bcl-2 in diffuse large B-cell lymphoma.Methods:This project used immunohistochemical methods to detect the expression of cyclinD2 and Bcl-2 in 120 cas...Objective:To study the expression and significance of cyclinD2 and Bcl-2 in diffuse large B-cell lymphoma.Methods:This project used immunohistochemical methods to detect the expression of cyclinD2 and Bcl-2 in 120 cases of diffuse large B-cell lymphoma and 80 cases of reactive hyperplasia of lymphoid tissue.The materials were collected from the hospital from January 2018 to 2020.In March 2015,120 patients had lymphoma tissue removed during the month of surgery.The postoperative pathological diagnosis was DLBCL.Another 80 cases of lymphoid hyperplasia(RLH)tissues were selected as controls.Results:CyclinD2 and BCL-2 expression were not statistically different in patients with diffuse large B-cell lymphoma in different ages,genders,locations,tissue types,and degree of differentiation;but statistically significant in different Ann Arbor stages,immunotypes,IPI index and first treatment efficacy.Conclusion:This research not only has important theoretical value,but also important economic value and social significance.展开更多
Objective:To study the expression and significance of cell cycle proteins CyclinD2,mPGES-1,Bcl2 in diffuse large B-cell lymphoma.Methods:Choose lymphoma and sexually hyper-plastic lymphoid tissues as control.Immunohis...Objective:To study the expression and significance of cell cycle proteins CyclinD2,mPGES-1,Bcl2 in diffuse large B-cell lymphoma.Methods:Choose lymphoma and sexually hyper-plastic lymphoid tissues as control.Immunohistoc-hemical methods were used to detect the expression of CyclinD2,mPGES-1,and Bcl2,and to compare the positive expression rates of CyclinD2,MPGES-1 and Bcl2 in diffuse large B-cell lymphoma and reactive proliferative lymphoid tissues to compare their diffusion formation.B-cell lymphoma was analyzed for its clinicopathological features.Results:The positive expression rate of CyclinD2,mPGES-1 and Bcl2 in diffuse large B-cell lymphoma is higher than that in reactive proliferative lymphoid tissue,and the difference between the two is statistically significant.There was no statistical difference in CyclinD2,mPGES-1 and Bcl2 in diffuse large B-cell lymphoma between patients according to the age,sex,location,tissue type and degree of differentiation.Conclusion:CyclinD2,mPGES-1 and Bcl2 are highly expressed in patients with diffuse large B-cell lymphoma,and can be used as reference indicators for evaluating the malignant degree and efficacy of dysplasia.展开更多
Objective:To explore the mechanism by which macrophages secrete CCL22 to promote the metastasis of triple-negative breast cancer under hypoxic conditions.Methods:20 ng/mL mass concentration of phorbol 12-myristate 13-...Objective:To explore the mechanism by which macrophages secrete CCL22 to promote the metastasis of triple-negative breast cancer under hypoxic conditions.Methods:20 ng/mL mass concentration of phorbol 12-myristate 13-acetate(PMA)cell culture medium,4′,6-diamidino-2-phenylindole(DAPI),dimethyl sulfoxide(DMSO),trypsin digestion solution,CCL18 Kit,Interleukin(IL)-10 Kit,CCL17 Kit,CCL22 Kit,TRIzol^(TM)Reagent Reverse Transcriptase Polymerase Chain Reaction(RT-PCR)Kit,triple-negative breast cancer cells MDA-MB-231 and BT-549,as well as other reagents were used to culture triple-negative breast cancer(TNBC)cells MDA-MB-231 and BT-549 as well as human mononuclear cells THP-1,analyze and observe the metastasis of triple-negative breast cancer cells to the lungs,the secretion of CCL22,the migration of triple-negative breast cancer,and the situation of CCR4.Results:Compared with normal tumor-associated macrophage(TAM),hypoxic TAM further promoted the migration of tumor cells.The number of tumor metastases in the lungs,induced by hypoxic TAM,was significantly higher than that of normoxic TAM.Hypoxia can significantly stimulate the expression of CCL22.CCL22 can significantly promote the migration of MDA-MB-231 and BT-549 cells.The expressions of CCR3,CCR4,and CCR5 in tumor tissues were significantly increased compared with normal tissues,in which CCR4 showed the most significant increase.Conclusion:TAM cultured under hypoxia significantly enhanced the migration ability of TNBC cells and promoted the metastasis of cancer cells to the lungs in vivo.The hypoxic condition induced TAM to secrete CCL22;the expression of CCL22 receptor,CCR4,in breast cancer tissues was significantly higher than that in normal tissues.展开更多
In recent years,there are increasingly more standardized trainings for doctors based on the standardized training outline that is suitable for different grades.Hierarchical training has gradually formed a relatively s...In recent years,there are increasingly more standardized trainings for doctors based on the standardized training outline that is suitable for different grades.Hierarchical training has gradually formed a relatively sound training mode.In reflecting students’learning effect,there is a need for an ideal quality feedback system in the standardized practical skills training for clinical pathology residents.This feedback system can help students achieve the training objectives through understanding and grasping the learning content.展开更多
Objective:To explore the regulatory mechanism of ginsenoside Rh1 on breast cancer cell extravasation based on CCL20-CCR6.Methods:In 2021,a total of 34 patients with breast cancer were treated in Baoding First Central ...Objective:To explore the regulatory mechanism of ginsenoside Rh1 on breast cancer cell extravasation based on CCL20-CCR6.Methods:In 2021,a total of 34 patients with breast cancer were treated in Baoding First Central Hospital.During hospitalization,pathological examinations were performed,and all the patients were diagnosed with invasive ductal carcinoma.Out of the 34 cases,16 cases were found to have CCR6 expression in breast cancer tissues,in which they were recorded as the CCR6 expression group,whereas 18 cases did not have CCR6 expression;these cases were recorded as the CCR6 non-expression group.During the same period,21 normal patients were selected as the control group.The peripheral blood CCL20 level and the expression of CCR6 on the surface of CD3+T lymphocytes were analyzed.The extracts of cancer cells were collected,purified,and cultured,and the effect of ginsenoside Rh1 on the invasion and metastasis of breast cancer cells was analyzed.Results:The peripheral blood CCL20 level in the CCR6 expression group was significantly higher than that in the CCR6 non-expression group and the control group,in which p<0.05,indicating that the difference was statistically significant;at 12,24,and 48 hours,the cell survival rate of each dose group was significantly higher than that of the blank control group and the dimethyl sulfoxide(DMSO)group(p<0.05).At 48 hours,comparing the low-dose group with the high-dose group,the cell survival rate significantly decreased(p<0.05).Compared with the blank control group and DMSO group,the invasion ability of breast cancer cells could be reduced in both,high-and medium-dose groups,where p<0.05,indicating that the difference was statistically significant.Conclusion:CCL20 may play a role in the pathogenesis of certain breast cancers,and ginsenoside Rh1 can effectively regulate the invasion and migration of breast cancer cells.展开更多
Objective:To determine the clinical value of combined detection of circulating tumor cells(CTCs)and cell-free DNA(cfDNA)in peripheral blood of patients with triple-negative breast cancer.Method:41 patients with breast...Objective:To determine the clinical value of combined detection of circulating tumor cells(CTCs)and cell-free DNA(cfDNA)in peripheral blood of patients with triple-negative breast cancer.Method:41 patients with breast cancer admitted to the First Central Hospital of Baoding from January 2020 to December 2021 were selected and recruited into the experimental group,42 patients with benign breast cancer admitted during the same period were recruited into the conditional control group,and 41 healthy patients admitted during the same period were recruited into the blank control group.The positive rate of peripheral blood CTCs,the level of cfDNA,and the diagnostic efficacy of peripheral blood CTCs,cfDNA alone and the combination thereof for breast cancer were analyzed.Result:The positive rates of peripheral blood CTCs in the experimental group,the conditional control group,and the blank control group were 43.90%,11.90%,and 9.74%,respectively,and there was significant difference among the groups.The levels of cfDNA in peripheral blood of the experimental group,the conditional control group,and the blank control group were 0.26±0.08 bp,0.17±0.03 bp,and 0.15±0.04 bp,respectively,which were statistically significant.The detection levels of 100 bp hTERT/ng mT1 and 241 bp hTERT/ng-mT1 in the experimental group were significantly higher than those in the conditional control group and the blank control group.The accuracy of peripheral blood CTCs detection in the three groups was 66.21%,the accuracy of cfDA241 bp/100 hp hTERT detection was 80.41%,and the accuracy of combined detection of peripheral blood CTCs and cfDNA was 94.03%.Conclusion:The clinical application of peripheral blood CTCs combined with cfDNA level detection can increase detection accuracy,provide data support for clinicians,and improve the clinical diagnostic effect of triple-negative breast cancer.展开更多
Adenoid basal carcinoma(ABC)of the cervix is a rare and low-incidence low-grade cervical cancer.In our practice,we encountered a case of cervical ABC with squamous differentiation and high-grade squamous intraepitheli...Adenoid basal carcinoma(ABC)of the cervix is a rare and low-incidence low-grade cervical cancer.In our practice,we encountered a case of cervical ABC with squamous differentiation and high-grade squamous intraepithelial lesion(HSIL)with gland involvement in the peripheral cervix.Reviewing relevant literature and analyzing its clinical manifestations,pathological morphology,and immunohistochemical characteristics would help deepen the understanding of this malignant tumor,so as to make a comprehensive diagnosis with differential diagnosis and prevent misdiagnosis.展开更多
Objective:To explore a method to solve the issue of interference in fluorescence quantitative PCR non-specific amplification for gene detection.Method:A three-step method was used for amplification,and the quantitativ...Objective:To explore a method to solve the issue of interference in fluorescence quantitative PCR non-specific amplification for gene detection.Method:A three-step method was used for amplification,and the quantitative fluorescence signal collection process was set in the extension stage.Results:Three-step amplification has the advantages of wide application range;improved accuracy;and reduced primer design requirements.Conclusion:The interference of non-specific amplification signals was effectively avoided,the melting curve plotting process was omitted,the reaction time was shortened,and the detection accuracy was improved.展开更多
Myofibroblastoma of the breast(MFB)is a rare benign mesenchymal tumor of the breast.A case of giant breast myofibroblastic tumor,which is rarely reported in literature,was recently diagnosed in our department.We also ...Myofibroblastoma of the breast(MFB)is a rare benign mesenchymal tumor of the breast.A case of giant breast myofibroblastic tumor,which is rarely reported in literature,was recently diagnosed in our department.We also analyzed the clinicopathological features of MFB to improve the understanding of the tumor and avoid misdiagnosis.展开更多
文摘Objective:To explore the detection and clinical significance of peripheral blood CTC,CA125,CA153,and CEA levels in patients with DCIS.Methods:210 patients who received surgical treatment for breast cancer in our hospital from January 2019 to December 2022 were selected as the research subjects.According to the postoperative pathology,100 cases were divided into breast cancer and 110 benign breast tumor groups.One hundred ten healthy patients undergoing physical examination during the same period were selected as the control group.CA153,CA125,and CEA levels were observed in the three groups.Results:The levels of CA153,CA125,and CEA in the breast cancer group were significantly higher than those in the benign breast tumor group and the control group(P<0.05);the levels of CA153,CA125,and CEA in the benign breast tumor group were all higher than those in the control group,but the differences were not statistically significant;among the three tumor markers,CA153 has the highest sensitivity at 39.00%,CA125 has the second highest sensitivity at 18.00%,and CEA has the lowest sensitivity at 17.00%;The sensitivity of the two joint tests of CA153+CA125,CA153+CEA,and CA125+CEA for the diagnosis of breast cancer were 50.00%,48.00%,and 26.00%respectively;the sensitivity of the three joint tests is the highest,reaching 53.00%,while the specificity of the joint tests was lower than the individual tests.Conclusion:Detection of peripheral blood CTC,CA125,CA153,and CEA levels has specific reference significance for the treatment and prognosis of DCIS patients.
文摘Objective:To explore the value of circulating free(cfDNA)content in the clinical diagnosis and treatment of triple-negative breast cancer(TNBC).Methods:A total of 39 TNBC patients,45 non-TNBC patients,and 50 healthy individuals admitted to the Baoding First Central Hospital during 2019-2022 were recruited.The clinical data,peripheral blood cfDNA concentration,and clinicopathological indicators of the patients were observed and analyzed.Results:The difference in clinical indicators such as age,age range,tumor size,clinical stage,and lymph node metastasis between patients with TNBC and non-TNBC was insignificant(P>0.05).The cfDNA concentrations(ng/mL)of the TNBC group,non-TNBC group,and healthy group were 24.12±4.98,15.36±4.12,and 3.12±1.02,respectively,and they are statistically different(P<0.05).The difference in cfDNA concentration was insignificant between TNBC patients with tumors≤2 cm and>2 cm(P>0.05)but was significant between TNBC patients with clinical stages I+II and III+IV(P<0.05).The cfDNA concentration in TNBC patients with lymph node metastasis was significantly higher than those without lymph node metastasis(P<0.05).Conclusion:cfDNA has an important application value in the diagnosis and treatment of breast cancer.By detecting the cfDNA level and its gene variation,valuable information about the progress and treatment effects of breast cancer can be obtained.This non-invasive detection method has a wide range of applications and can be used for early screening,auxiliary diagnosis,efficacy evaluation,and recurrence monitoring of breast cancer.
基金Hebei Province Medical Science Research Plan Project(20220282)Baoding Science and Technology Plan Project(2141ZF237)。
文摘The endoplasmic reticulum plays an extremely important role in the process of cellular protein secretion.The cyclic AMP-responsive element-binding protein 3(CREB3)transcription factor family is closely associated with the secretion and transport of proteins within the endoplasmic reticulum.As a member of the CREB3 transcription factor family,cyclic AMP-responsive element-binding protein 3-like protein 2(CREB3L2)stands out as a non-classical sensor within the endoplasmic reticulum.CREB3L2 can detect and regulate endoplasmic reticulum pressure,exert control over the processes of protein transport and secretion,participate in the development of tumor cells,and is also closely linked to the development of certain human tissues and organs.This article aims to review the role of CREB3L2 in tissue development and disease,shedding light on the related mechanisms of CREB3L2 in cancer development.The goal is to provide insights and directions for further analysis of CREB3L2.
文摘Pathology is the gold standard for clinical diagnosis and an important bridge between clinical work and basic disciplines.Therefore,training qualified clinical pathologists is very important for clinical work.It is challenging to diagnose lymphohematopoietic diseases in practice since they made up a large percentage of resident doctors’standardized training and there are numerous knowledge nodes associated with them.Therefore,this training base adopts the“case-introduction”teaching mode to help resident doctors master the knowledge nodes required by the outline in the study of lymphohematopoietic system pathology.
基金Hebei Province:Beijing Tianjin Hebei basic research cooperation project(2019),Project No.19JCZDJC65800(Z).
文摘In many countries,colorectal cancer is one of the common cancers.It has been confirmed that microsatellite instability(MSI)is one of the most important molecular changes in colorectal cancer.As the recognized precursor lesions of colorectal cancer,adenomas and some polyps also have MSI phenomenon.This article analyzes the MSI of colorectal cancer and its closely related adenomas and polyps in domestic and foreign literature,and further discusses how to identify colorectal cancer at an early stage and the impact of MSI on the prognosis and treatment of colorectal cancer.
基金Project supported by Natural Science Foundation of Hebei Province:Beijing-Tianjin-Hebei Basic Research Cooperation Special Project(2019):Visualization Stem Cell Targeted Tumor Therapy Technology for Accurate Diagnosis and Treatment of Tumors Project No.:19JCZDJC65800(Z)。
文摘Circulating free DNA(cfDNA)refers to the free DNA nucleotides in body fluids,but the dynamic response in vivo tumor load and full assessment of genetic heterogeneity in cancer patients provides a non-invasive diagnostic approach.It is one of the effective biomarkers for cancer screening and diagnosis,and also provides for the prognosis for patients with advanced cancer and forecast information.In this paper,the clinical application of cfDNA is reviewed.
文摘Objective:To compare the effects of clinical application of chemiluminescence and real-time,fluorescence-based quantitative PCR in the detection Epstein-Barr virus(EBV).Methods:The data of chemiluminescence and real-time fluorescent quantitative PCR.fromipaEsfwo were suspected of being infectea w1tn rito1 roro January 2016 to January 2019 in our hospital were analyzed.The specific stage of EBV infection was analyzed,and the differences in results of the two detection methods were compared.Results:Chemiluminescence method was used to detect EBV infection during the active phase.The sensitivity of the chemiluminescence method was 76.7%(56/73)and the real-time quantitative PCRmethod was 90.4%(66/73).There was a statistical difference between the two detection methods(P<0.05).Conclusion:There was no statistical difference in positive predictive values between the chemiluminescence method and the real-time,fluorescence-based quantitative PCR method in the detection of EBV infection,but the sensitivity of chemiluminescence method is slightly lower than the real-time quantitative PCRmethod.It is noteworthy that chemiluminescence method is convenient and fast while the real-time,fluorescence-based quantitative PCR method is more accurate,which can provide a more accurate reference for clinical treatment.
基金The Health and Biomedical Special Project of Hebei Province Key R&D Project(2018)(18277732D)。
文摘Objective:To explore the expression of human myxovirus resistance protein A(MxA)in triple negative breast cancer(TNBC)and its relationship with prognosis.Methods:144 cases of TNBC confirmed by pathology before or after surgery from January 2014 to January 2017 in the First Central Hospital of Baoding City were retrospectively collected.Western blotting was used to detect the expression of MxA protein in TNBC and adjacent breast tissues.According to the expression of MxA protein,144 TNBC patients were divided into low MxA protein expression group(n=91)and MxA protein high expression group(n=53)for subsequent comparison of prognosis of patients in between these two groups.Results:The expression of MxA protein in TNBC tissue was lower than that of adjacent breast tissue,and the difference was statistically significant(P<0.05).The patients in high MxA expression group had higher loco-regional recurrence-free rate,disease-free survival(DFS)rate,and overall survival(OS)rate than those in low MxA expression group for 3 years.On the other hand,the distant metastasis rate was lower in the high expression group compared to that in the low MxA expression group,and the difference was statistically significant(P<0.05).Conclusion:In triple-negative breast cancer,high MxA expression is a potential predictor of TNBC prognosis.
文摘Objective:To study the expression and significance of cyclinD2 and Bcl-2 in diffuse large B-cell lymphoma.Methods:This project used immunohistochemical methods to detect the expression of cyclinD2 and Bcl-2 in 120 cases of diffuse large B-cell lymphoma and 80 cases of reactive hyperplasia of lymphoid tissue.The materials were collected from the hospital from January 2018 to 2020.In March 2015,120 patients had lymphoma tissue removed during the month of surgery.The postoperative pathological diagnosis was DLBCL.Another 80 cases of lymphoid hyperplasia(RLH)tissues were selected as controls.Results:CyclinD2 and BCL-2 expression were not statistically different in patients with diffuse large B-cell lymphoma in different ages,genders,locations,tissue types,and degree of differentiation;but statistically significant in different Ann Arbor stages,immunotypes,IPI index and first treatment efficacy.Conclusion:This research not only has important theoretical value,but also important economic value and social significance.
基金Qiushuang Ma and Peng Lin both are the first authors and contributed equally to this work.*Jinku Zhang and Jirui Sun are the corresponding authors and contributed equally to this work。
文摘Objective:To study the expression and significance of cell cycle proteins CyclinD2,mPGES-1,Bcl2 in diffuse large B-cell lymphoma.Methods:Choose lymphoma and sexually hyper-plastic lymphoid tissues as control.Immunohistoc-hemical methods were used to detect the expression of CyclinD2,mPGES-1,and Bcl2,and to compare the positive expression rates of CyclinD2,MPGES-1 and Bcl2 in diffuse large B-cell lymphoma and reactive proliferative lymphoid tissues to compare their diffusion formation.B-cell lymphoma was analyzed for its clinicopathological features.Results:The positive expression rate of CyclinD2,mPGES-1 and Bcl2 in diffuse large B-cell lymphoma is higher than that in reactive proliferative lymphoid tissue,and the difference between the two is statistically significant.There was no statistical difference in CyclinD2,mPGES-1 and Bcl2 in diffuse large B-cell lymphoma between patients according to the age,sex,location,tissue type and degree of differentiation.Conclusion:CyclinD2,mPGES-1 and Bcl2 are highly expressed in patients with diffuse large B-cell lymphoma,and can be used as reference indicators for evaluating the malignant degree and efficacy of dysplasia.
基金supported by grants from The Medical Science Research Project of Hebei Province(20220282)the Key Laboratory of Molecular Pathology and Early Diagnosis of Tumor in Hebei Province.
文摘Objective:To explore the mechanism by which macrophages secrete CCL22 to promote the metastasis of triple-negative breast cancer under hypoxic conditions.Methods:20 ng/mL mass concentration of phorbol 12-myristate 13-acetate(PMA)cell culture medium,4′,6-diamidino-2-phenylindole(DAPI),dimethyl sulfoxide(DMSO),trypsin digestion solution,CCL18 Kit,Interleukin(IL)-10 Kit,CCL17 Kit,CCL22 Kit,TRIzol^(TM)Reagent Reverse Transcriptase Polymerase Chain Reaction(RT-PCR)Kit,triple-negative breast cancer cells MDA-MB-231 and BT-549,as well as other reagents were used to culture triple-negative breast cancer(TNBC)cells MDA-MB-231 and BT-549 as well as human mononuclear cells THP-1,analyze and observe the metastasis of triple-negative breast cancer cells to the lungs,the secretion of CCL22,the migration of triple-negative breast cancer,and the situation of CCR4.Results:Compared with normal tumor-associated macrophage(TAM),hypoxic TAM further promoted the migration of tumor cells.The number of tumor metastases in the lungs,induced by hypoxic TAM,was significantly higher than that of normoxic TAM.Hypoxia can significantly stimulate the expression of CCL22.CCL22 can significantly promote the migration of MDA-MB-231 and BT-549 cells.The expressions of CCR3,CCR4,and CCR5 in tumor tissues were significantly increased compared with normal tissues,in which CCR4 showed the most significant increase.Conclusion:TAM cultured under hypoxia significantly enhanced the migration ability of TNBC cells and promoted the metastasis of cancer cells to the lungs in vivo.The hypoxic condition induced TAM to secrete CCL22;the expression of CCL22 receptor,CCR4,in breast cancer tissues was significantly higher than that in normal tissues.
文摘In recent years,there are increasingly more standardized trainings for doctors based on the standardized training outline that is suitable for different grades.Hierarchical training has gradually formed a relatively sound training mode.In reflecting students’learning effect,there is a need for an ideal quality feedback system in the standardized practical skills training for clinical pathology residents.This feedback system can help students achieve the training objectives through understanding and grasping the learning content.
基金supported by grants from The Medical Science Research Project of Hebei Province(20220282)the Key Laboratory of Molecular Pathology and Early Diagnosis of Tumor in Hebei Province.
文摘Objective:To explore the regulatory mechanism of ginsenoside Rh1 on breast cancer cell extravasation based on CCL20-CCR6.Methods:In 2021,a total of 34 patients with breast cancer were treated in Baoding First Central Hospital.During hospitalization,pathological examinations were performed,and all the patients were diagnosed with invasive ductal carcinoma.Out of the 34 cases,16 cases were found to have CCR6 expression in breast cancer tissues,in which they were recorded as the CCR6 expression group,whereas 18 cases did not have CCR6 expression;these cases were recorded as the CCR6 non-expression group.During the same period,21 normal patients were selected as the control group.The peripheral blood CCL20 level and the expression of CCR6 on the surface of CD3+T lymphocytes were analyzed.The extracts of cancer cells were collected,purified,and cultured,and the effect of ginsenoside Rh1 on the invasion and metastasis of breast cancer cells was analyzed.Results:The peripheral blood CCL20 level in the CCR6 expression group was significantly higher than that in the CCR6 non-expression group and the control group,in which p<0.05,indicating that the difference was statistically significant;at 12,24,and 48 hours,the cell survival rate of each dose group was significantly higher than that of the blank control group and the dimethyl sulfoxide(DMSO)group(p<0.05).At 48 hours,comparing the low-dose group with the high-dose group,the cell survival rate significantly decreased(p<0.05).Compared with the blank control group and DMSO group,the invasion ability of breast cancer cells could be reduced in both,high-and medium-dose groups,where p<0.05,indicating that the difference was statistically significant.Conclusion:CCL20 may play a role in the pathogenesis of certain breast cancers,and ginsenoside Rh1 can effectively regulate the invasion and migration of breast cancer cells.
基金This present study was financially supported by Baoding City Science and Technology Plan project(2041ZF084)Key Laboratory of Molecular Pathology and Early Diagnosis of Tumor in Hebei Province.
文摘Objective:To determine the clinical value of combined detection of circulating tumor cells(CTCs)and cell-free DNA(cfDNA)in peripheral blood of patients with triple-negative breast cancer.Method:41 patients with breast cancer admitted to the First Central Hospital of Baoding from January 2020 to December 2021 were selected and recruited into the experimental group,42 patients with benign breast cancer admitted during the same period were recruited into the conditional control group,and 41 healthy patients admitted during the same period were recruited into the blank control group.The positive rate of peripheral blood CTCs,the level of cfDNA,and the diagnostic efficacy of peripheral blood CTCs,cfDNA alone and the combination thereof for breast cancer were analyzed.Result:The positive rates of peripheral blood CTCs in the experimental group,the conditional control group,and the blank control group were 43.90%,11.90%,and 9.74%,respectively,and there was significant difference among the groups.The levels of cfDNA in peripheral blood of the experimental group,the conditional control group,and the blank control group were 0.26±0.08 bp,0.17±0.03 bp,and 0.15±0.04 bp,respectively,which were statistically significant.The detection levels of 100 bp hTERT/ng mT1 and 241 bp hTERT/ng-mT1 in the experimental group were significantly higher than those in the conditional control group and the blank control group.The accuracy of peripheral blood CTCs detection in the three groups was 66.21%,the accuracy of cfDA241 bp/100 hp hTERT detection was 80.41%,and the accuracy of combined detection of peripheral blood CTCs and cfDNA was 94.03%.Conclusion:The clinical application of peripheral blood CTCs combined with cfDNA level detection can increase detection accuracy,provide data support for clinicians,and improve the clinical diagnostic effect of triple-negative breast cancer.
文摘Adenoid basal carcinoma(ABC)of the cervix is a rare and low-incidence low-grade cervical cancer.In our practice,we encountered a case of cervical ABC with squamous differentiation and high-grade squamous intraepithelial lesion(HSIL)with gland involvement in the peripheral cervix.Reviewing relevant literature and analyzing its clinical manifestations,pathological morphology,and immunohistochemical characteristics would help deepen the understanding of this malignant tumor,so as to make a comprehensive diagnosis with differential diagnosis and prevent misdiagnosis.
文摘Objective:To explore a method to solve the issue of interference in fluorescence quantitative PCR non-specific amplification for gene detection.Method:A three-step method was used for amplification,and the quantitative fluorescence signal collection process was set in the extension stage.Results:Three-step amplification has the advantages of wide application range;improved accuracy;and reduced primer design requirements.Conclusion:The interference of non-specific amplification signals was effectively avoided,the melting curve plotting process was omitted,the reaction time was shortened,and the detection accuracy was improved.
基金The Beijing-Tianjin-Hebei Basic Research Cooperation Special Project(2019):"Visualized Stem Cell Targeted Tumor Therapy Techniques for Precise Diagnosis and Treatment of Tumors"Project Number:19JCZDJC65800(Z).
文摘Myofibroblastoma of the breast(MFB)is a rare benign mesenchymal tumor of the breast.A case of giant breast myofibroblastic tumor,which is rarely reported in literature,was recently diagnosed in our department.We also analyzed the clinicopathological features of MFB to improve the understanding of the tumor and avoid misdiagnosis.
