Background Goose, descendants of migratory ancestors, have undergone extensive selective breeding, resulting in their remarkable ability to accumulate fat in the liver and exhibit a high tolerance for significant ener...Background Goose, descendants of migratory ancestors, have undergone extensive selective breeding, resulting in their remarkable ability to accumulate fat in the liver and exhibit a high tolerance for significant energy intake. As a result, goose offers an excellent model for studying obesity, metabolic disorders, and liver diseases in mammals. Although the impact of the three-dimensional arrangement of chromatin within the cell nucleus on gene expression and transcriptional regulation is widely acknowledged, the precise functions of chromatin architecture reorganization during fat deposition in goose liver tissues still need to be fully comprehended.Results In this study, geese exhibited more pronounced changes in the liver index and triglyceride(TG) content following the consumption of the high-fat diet(HFD) than mice without significant signs of inflammation. Additionally, we performed comprehensive analyses on 10 goose liver tissues(5 HFD, 5 normal), including generating highresolution maps of chromatin architecture, conducting whole-genome gene expression profiling, and identifying H3K27ac peaks in the livers of geese and mice subjected to the HFD. Our results unveiled a multiscale restructuring of chromatin architecture, encompassing Compartment A/B, topologically associated domains, and interactions between promoters and enhancers. The dynamism of the three-dimensional genome architecture, prompted by the HFD, assumed a pivotal role in the transcriptional regulation of crucial genes. Furthermore, we identified genes that regulate chromatin conformation changes, contributing to the metabolic adaptation process of lipid deposition and hepatic fat changes in geese in response to excessive energy intake. Moreover, we conducted a cross-species analysis comparing geese and mice exposed to the HFD, revealing unique characteristics specific to the goose liver compared to a mouse. These chromatin conformation changes help elucidate the observed characteristics of fat deposition and hepatic fat regulation in geese under conditions of excessive energy intake.Conclusions We examined the dynamic modifications in three-dimensional chromatin architecture and gene expression induced by an HFD in goose liver tissues. We conducted a cross-species analysis comparing that of mice. Our results contribute significant insights into the chromatin architecture of goose liver tissues, offering a novel perspective for investigating mammal liver diseases.展开更多
With the rapid development of urbanization and the lack of sustainable regulation policies,large cities have become a critical pollution source to urban rivers.To elucidate the pollution status of the Nanming River,an...With the rapid development of urbanization and the lack of sustainable regulation policies,large cities have become a critical pollution source to urban rivers.To elucidate the pollution status of the Nanming River,an urban river in Guiyang City,SW China,water samples were collected along the river from the upstream(through the urbanization area)to the downstream locations.Pollution levels of heavy metals,NH4+and total phosphorus(TP)were investigated,and dissolved organic matter(DOM)was analyzed with fluorescence spectrophotometer.The results reveal that Huangjindadao,a local famous scenic spot,is a notable pollution source to the Nanming River,and the Huaxi National City Wetland Park plays an important role in protection of the water quality of this river.Analysis of 3DEEM characteristics of DOM indicated that the main pollution sources of DOM in this water body was municipal waste water.Protein-like compounds and humic acid with high molecular weight was the main components of DOM in this river.Organic compounds with low molecular weight represented a very small portion of the DOM in water of the Nanming River,especially in the downstream part of this river.展开更多
In South China,the tropical weather and climate result in severe sugarcane lodging problems which greatly hinders sugarcane mechanical harvesting.The main function of the crop divider is to lift lodged canes and facil...In South China,the tropical weather and climate result in severe sugarcane lodging problems which greatly hinders sugarcane mechanical harvesting.The main function of the crop divider is to lift lodged canes and facilitate base-cut cane feeding.In this study,a series of field tests were conducted on crop dividers of Liugong 4GQ-180 sugarcane chopper harvester.The tests were designed to identify the relationships of the performance index(maximum lifting height of sugarcane)and the main influencing factors(machine forward speed,rotational speed of inside scrolls,and ground clearance of crop divider toes).The single factor test was conducted to determine optimum range of these test factors:the forward speed(FS)was 2.0-4.0 km/h,the rotational speed of inside scrolls(RS)was 100-140 r/min,and the toe ground clearance(GC)was 0-5 cm.The orthogonal test(three factors and three levels)was carried out to determine the optimal combination of operating parameters:FS was 2.0 km/h,RS was 115 r/min,and GC was 0 cm.In addition,the range analysis of orthogonal test results revealed that the order of each test factor affecting crop divider performance was RS,FS,GC.This study was expected to provide valuable references for the optimal operation of sugarcane harvester crop dividers.展开更多
基金supported by the National Key R&D Program of China (2022YFF1000100 to Long Jin and 2023YFD1300012 to Long Jin)the Sichuan Science and Technology Program (2022JDJQ0054 to Long Jin and 2021YFYZ0009 to Mingzhou Li)the National Natural Science Foundation of China (32225046 to Mingzhou Li)。
文摘Background Goose, descendants of migratory ancestors, have undergone extensive selective breeding, resulting in their remarkable ability to accumulate fat in the liver and exhibit a high tolerance for significant energy intake. As a result, goose offers an excellent model for studying obesity, metabolic disorders, and liver diseases in mammals. Although the impact of the three-dimensional arrangement of chromatin within the cell nucleus on gene expression and transcriptional regulation is widely acknowledged, the precise functions of chromatin architecture reorganization during fat deposition in goose liver tissues still need to be fully comprehended.Results In this study, geese exhibited more pronounced changes in the liver index and triglyceride(TG) content following the consumption of the high-fat diet(HFD) than mice without significant signs of inflammation. Additionally, we performed comprehensive analyses on 10 goose liver tissues(5 HFD, 5 normal), including generating highresolution maps of chromatin architecture, conducting whole-genome gene expression profiling, and identifying H3K27ac peaks in the livers of geese and mice subjected to the HFD. Our results unveiled a multiscale restructuring of chromatin architecture, encompassing Compartment A/B, topologically associated domains, and interactions between promoters and enhancers. The dynamism of the three-dimensional genome architecture, prompted by the HFD, assumed a pivotal role in the transcriptional regulation of crucial genes. Furthermore, we identified genes that regulate chromatin conformation changes, contributing to the metabolic adaptation process of lipid deposition and hepatic fat changes in geese in response to excessive energy intake. Moreover, we conducted a cross-species analysis comparing geese and mice exposed to the HFD, revealing unique characteristics specific to the goose liver compared to a mouse. These chromatin conformation changes help elucidate the observed characteristics of fat deposition and hepatic fat regulation in geese under conditions of excessive energy intake.Conclusions We examined the dynamic modifications in three-dimensional chromatin architecture and gene expression induced by an HFD in goose liver tissues. We conducted a cross-species analysis comparing that of mice. Our results contribute significant insights into the chromatin architecture of goose liver tissues, offering a novel perspective for investigating mammal liver diseases.
文摘With the rapid development of urbanization and the lack of sustainable regulation policies,large cities have become a critical pollution source to urban rivers.To elucidate the pollution status of the Nanming River,an urban river in Guiyang City,SW China,water samples were collected along the river from the upstream(through the urbanization area)to the downstream locations.Pollution levels of heavy metals,NH4+and total phosphorus(TP)were investigated,and dissolved organic matter(DOM)was analyzed with fluorescence spectrophotometer.The results reveal that Huangjindadao,a local famous scenic spot,is a notable pollution source to the Nanming River,and the Huaxi National City Wetland Park plays an important role in protection of the water quality of this river.Analysis of 3DEEM characteristics of DOM indicated that the main pollution sources of DOM in this water body was municipal waste water.Protein-like compounds and humic acid with high molecular weight was the main components of DOM in this river.Organic compounds with low molecular weight represented a very small portion of the DOM in water of the Nanming River,especially in the downstream part of this river.
基金The research presented in this work was partially supported by the Major Program of the National Natural Science Foundation of China(Grant No.32071916)the Major State Research Development Program of China(Grant No.2016YFD0701200)+1 种基金and China Agricultural University,Institute for New Rural Development Guangxi Fusui Professor Workstation Grant(No.201805510710115)Any opinions,findings,and conclusions expressed in this work are those of the authors and do not necessarily reflect the views of China Agricultural University.The authors acknowledge Mr.Xiangwei Li,Mr.Xiaobin Huang,Mr.Yuanmin Gan and Mr.Pingang Wu for their help in the harvesting test.
文摘In South China,the tropical weather and climate result in severe sugarcane lodging problems which greatly hinders sugarcane mechanical harvesting.The main function of the crop divider is to lift lodged canes and facilitate base-cut cane feeding.In this study,a series of field tests were conducted on crop dividers of Liugong 4GQ-180 sugarcane chopper harvester.The tests were designed to identify the relationships of the performance index(maximum lifting height of sugarcane)and the main influencing factors(machine forward speed,rotational speed of inside scrolls,and ground clearance of crop divider toes).The single factor test was conducted to determine optimum range of these test factors:the forward speed(FS)was 2.0-4.0 km/h,the rotational speed of inside scrolls(RS)was 100-140 r/min,and the toe ground clearance(GC)was 0-5 cm.The orthogonal test(three factors and three levels)was carried out to determine the optimal combination of operating parameters:FS was 2.0 km/h,RS was 115 r/min,and GC was 0 cm.In addition,the range analysis of orthogonal test results revealed that the order of each test factor affecting crop divider performance was RS,FS,GC.This study was expected to provide valuable references for the optimal operation of sugarcane harvester crop dividers.
