LIN28A,an RNA-binding protein,plays an important role in porcine induced pluripotent stem cells(piPSCs).However,the molecular mechanism underlying the function of LIN28A in the maintenance of pluripotency in piPSCs re...LIN28A,an RNA-binding protein,plays an important role in porcine induced pluripotent stem cells(piPSCs).However,the molecular mechanism underlying the function of LIN28A in the maintenance of pluripotency in piPSCs remains unclear.Here,we explored the function of LIN28A in piPSCs based on its overexpression and knockdown.We performed total RNA sequencing(RNA-seq)of piPSCs and detected the expression levels of relevant genes by quantitative real-time polymerase chain reaction(qRT-PCR),western blot analysis,and immunofluorescence staining.Results indicated that piPSC proliferation ability decreased following LIN28A knockdown.Furthermore,when LIN28A expression in the shLIN28A2 group was lower(by 20%)than that in the negative control knockdown group(shNC),the pluripotency of piPSCs disappeared and they differentiated into neuroectoderm cells.Results also showed that LIN28A overexpression inhibited the expression of DUSP(dual-specificity phosphatases)family phosphatases and activated the mitogen-activated protein kinase(MAPK)signaling pathway.Thus,LIN28A appears to activate the MAPK signaling pathway to maintain the pluripotency and proliferation ability of piPSCs.Our study provides a new resource for exploring the functions of LIN28A in piPSCs.展开更多
DEAR EDITOR,The use of CRISPR/Cas9 technology to breed polygenicmodified animals with desired traits holds great promise in agriculture and biomedicine. However, as most studies are based on human and mouse models, wh...DEAR EDITOR,The use of CRISPR/Cas9 technology to breed polygenicmodified animals with desired traits holds great promise in agriculture and biomedicine. However, as most studies are based on human and mouse models, whether different single guide RNA(sg RNA) expression strategies affect multiplex gene-editing efficiency in domestic animals remains elusive.展开更多
基金This work was supported by the National Key Research,Development Program of China-Stem Cell and Translational Research(2016YFA0100200)National Natural Science Foundation of China(32072806,31572399,61772431,62072377)+1 种基金Program of Shaanxi Province Science and Technology Innovation Team(2019TD-036)Fundamental Research Funds for the Central Universities,Northwest A&F University(Z1090219146,Z102022004)。
文摘LIN28A,an RNA-binding protein,plays an important role in porcine induced pluripotent stem cells(piPSCs).However,the molecular mechanism underlying the function of LIN28A in the maintenance of pluripotency in piPSCs remains unclear.Here,we explored the function of LIN28A in piPSCs based on its overexpression and knockdown.We performed total RNA sequencing(RNA-seq)of piPSCs and detected the expression levels of relevant genes by quantitative real-time polymerase chain reaction(qRT-PCR),western blot analysis,and immunofluorescence staining.Results indicated that piPSC proliferation ability decreased following LIN28A knockdown.Furthermore,when LIN28A expression in the shLIN28A2 group was lower(by 20%)than that in the negative control knockdown group(shNC),the pluripotency of piPSCs disappeared and they differentiated into neuroectoderm cells.Results also showed that LIN28A overexpression inhibited the expression of DUSP(dual-specificity phosphatases)family phosphatases and activated the mitogen-activated protein kinase(MAPK)signaling pathway.Thus,LIN28A appears to activate the MAPK signaling pathway to maintain the pluripotency and proliferation ability of piPSCs.Our study provides a new resource for exploring the functions of LIN28A in piPSCs.
基金supported by the National Natural Science Foundation of China(32072806)Shaanxi Province Science and Technology Innovation Team(2019TD-036)+1 种基金Key Program of Shaanxi Province Science and Technology(2022NY-044)。
文摘DEAR EDITOR,The use of CRISPR/Cas9 technology to breed polygenicmodified animals with desired traits holds great promise in agriculture and biomedicine. However, as most studies are based on human and mouse models, whether different single guide RNA(sg RNA) expression strategies affect multiplex gene-editing efficiency in domestic animals remains elusive.
