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Epitope Analysis of Anti-SARS-CoV-2 Neutralizing Antibodies 被引量:1
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作者 jun-biao xue Sheng-ce TAO 《Current Medical Science》 SCIE CAS 2021年第6期1065-1074,共10页
Coronavirus disease 2019 is threatening thousands of millions of people around the world.In the absence of specific and highly effective medicines,the treatment of infected persons is still very challenging.As therape... Coronavirus disease 2019 is threatening thousands of millions of people around the world.In the absence of specific and highly effective medicines,the treatment of infected persons is still very challenging.As therapeutics,neutralizing antibodies(NAbs)have great potential.Many NAbs have been reported,and most target various regions on the receptor-binding domain of the spike(S)protein,or the N-terminal domain.Several NAbs and NAb cocktails have been authorized for emergency use,and more arc in clinical trials or are under development.In this review,considering the angle of binding epitopes on the S protein,we summarize the functions and the underlying mechanisms of a set of well-recognized NAbs and provide guidance for vaccine design and the combinatorial use of these antibodies.In addition,we review the NAbs and NAb cocktails that have been approved for emergency use and discuss the effectiveness of these NAbs for combating severe acute respiratory syndrome coronavirus 2 mutants. 展开更多
关键词 coronavirus disease 2019 severe acute respiratory syndrome coronavirus 2 neutralizing antibody EPITOPE THERAPEUTICS VACCINE
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Specific pupylation as IDEntity reporter(SPIDER)for the identification of protein-biomolecule interactions 被引量:1
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作者 He-Wei Jiang Hong Chen +26 位作者 Yun-Xiao Zheng xue-Ning Wang Qingfeng Meng Jin Xie Jiong Zhang ChangSheng Zhang Zhao-Wei Xu Zi-Qing Chen Lei Wang Wei-Sha Kong Kuan Zhou Ming-Liang Ma Hai-Nan Zhang Shu-Juan Guo jun-biao xue Jing-Li Hou Zhe-Yi Liu Wen-xue Niu Fang-Jun Wang Tao Wang Wei Li Rui-Na Wang Yong-Jun Dang Daniel MCzajkowsky JianFeng Pei Jia-Jia Dong Sheng-Ce Tao 《Science China(Life Sciences)》 SCIE CAS CSCD 2023年第8期1869-1887,共19页
Protein-biomolecule interactions play pivotal roles in almost all biological processes.For a biomolecule of interest,the identification of the interacting protein(s)is essential.For this need,although many assays are ... Protein-biomolecule interactions play pivotal roles in almost all biological processes.For a biomolecule of interest,the identification of the interacting protein(s)is essential.For this need,although many assays are available,highly robust and reliable methods are always desired.By combining a substrate-based proximity labeling activity from the pupylation pathway of Mycobacterium tuberculosis and the streptavidin(SA)-biotin system,we developed the Specific Pupylation as IDEntity Reporter(SPIDER)method for identifying protein-biomolecule interactions.Using SPIDER,we validated the interactions between the known binding proteins of protein,DNA,RNA,and small molecule.We successfully applied SPIDER to construct the global protein interactome for m^(6)A and m RNA,identified a variety of uncharacterized m^(6)A binding proteins,and validated SRSF7 as a potential m^(6)A reader.We globally identified the binding proteins for lenalidomide and Cob B.Moreover,we identified SARS-CoV-2-specific receptors on the cell membrane.Overall,SPIDER is powerful and highly accessible for the study of proteinbiomolecule interactions. 展开更多
关键词 proximity labeling protein-biomolecule interaction proteomics pupylation
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Systematic evaluation of IgG responses to SARS-CoV-2 spike protein-derived peptides for monitoring COVID-19 patients 被引量:2
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作者 Yang Li Dan-yun La +30 位作者 Qing Lei Zhao-wei Xu Feng Wang Hongyan Hou Lingyun Chen Jiaoxiang Wu Yan Ren Ming-liang Ma Bo Zhang Hong Chen Caizheng Yu jun-biao xue Yun-xiao Zheng xue-ning Wang He-wei Jiang Hai-nan Zhang Huan Qi Shu-juan Guo Yandi Zhang Xiaosong Lin Zongjie Yao Pengfei Pang Dawei Shi Wei Wang Xiao Yang Jie Zhou Huiming Sheng Ziyong Sun Hong Shan Xionglin Fan Sheng-ce Tao 《Cellular & Molecular Immunology》 SCIE CAS CSCD 2021年第3期621-631,共11页
Serological tests play an essential role in monitoring and combating the COVID-19 pandemic.Recombinant spike protein(S protein),especially the S1 protein,is one of the major reagents used for serological tests.However... Serological tests play an essential role in monitoring and combating the COVID-19 pandemic.Recombinant spike protein(S protein),especially the S1 protein,is one of the major reagents used for serological tests.However,the high cost of S protein production and possible cross-reactivity with other human coronaviruses pose unavoidable challenges.By taking advantage of a peptide microarray with full spike protein coverage,we analyzed 2,434 sera from 858 COVID-19 patients,63 asymptomatic patients and 610 controls collected from multiple clinical centers.Based on the results,we identified several S protein-derived 12-mer peptides that have high diagnostic performance.In particular,for monitoring the IgG response,one peptide(aa 1148-1159 or S2-78)exhibited a sensitivity(95.5%,95%CI 93.7-96.9%)and specificity(96.7%,95%CI 94.8-98.0%)comparable to those of the S1 protein for the detection of both symptomatic and asymptomatic COVID-19 cases.Furthermore,the diagnostic performance of the S2-78(aa 1148-1159)IgG was successfully validated by ELISA in an independent sample cohort.A panel of four peptides,S1-93(aa 553-564),S1-97(aa 577-588),S1-101(aa 601-612)and S1-105(aa 625-636),that likely will avoid potential cross-reactivity with sera from patients infected by other coronaviruses was constructed.The peptides identified in this study may be applied independently or in combination with the S1 protein for accurate,affordable,and accessible COVID-19 diagnosis. 展开更多
关键词 SARS-CoV-2 SPIKE PEPTIDE ANTIBODY Diagnosis
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COVID-ONE-hi:The One-stop Database for COVID-19-specific Humoral Immunity and Clinical Parameters
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作者 Zhaowei Xu Yang Li +25 位作者 Qing Lei Likun Huang Dan-yun Lai Shu-juan Guo He-wei Jiang Hongyan Hou Yun-xiao Zheng xue-ning Wang Jiaoxiang Wu Ming-liang Ma Bo Zhang Hong Chen Caizheng Yu jun-biao xue Hai-nan Zhang Huan Qi Siqi Yu Mingxi Lin Yandi Zhang Xiaosong Lin Zongjie Yao Huiming Sheng Ziyong Sun Feng Wang Xionglin Fan Sheng-ce Tao 《Genomics, Proteomics & Bioinformatics》 SCIE CAS CSCD 2021年第5期669-678,共10页
Coronavirus disease 2019(COVID-19),which is caused by SARS-CoV-2,varies with regard to symptoms and mortality rates among populations.Humoral immunity plays critical roles in SARS-CoV-2 infection and recovery from COV... Coronavirus disease 2019(COVID-19),which is caused by SARS-CoV-2,varies with regard to symptoms and mortality rates among populations.Humoral immunity plays critical roles in SARS-CoV-2 infection and recovery from COVID-19.However,differences in immune responses and clinical features among COVID-19 patients remain largely unknown.Here,we report a database for COVID-19-specific IgG/IgM immune responses and clinical parameters(named COVID-ONE-hi).COVID-ONE-hi is based on the data that contain the IgG/IgM responses to 24 full-length/truncated proteins corresponding to 20 of 28 known SARS-CoV-2 proteins and 199 spike protein peptides against 2360 serum samples collected from 783 COVID-19 patients.In addition,96 clinical parameters for the 2360 serum samples and basic information for the 783 patients are integrated into the database.Furthermore,COVID-ONE-hi provides a dashboard for defining samples and a one-click analysis pipeline for a single group or paired groups.A set of samples of interest is easily defined by adjusting the scale bars of a variety of parameters.After the“START”button is clicked,one can readily obtain a comprehensive analysis report for further interpretation.COVID-ONE-hi is freely available at www.COVID-ONE.cn. 展开更多
关键词 SARS-CoV-2 Protein microarray Humoral immunity One-stop tool Shiny
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