GF-14 satellite is a new generation of sub-meter stereo surveying and mapping satellite in China,carrying dual-line array stereo mapping cameras to achieve 1∶10000 scale topographic mapping without Ground Control Poi...GF-14 satellite is a new generation of sub-meter stereo surveying and mapping satellite in China,carrying dual-line array stereo mapping cameras to achieve 1∶10000 scale topographic mapping without Ground Control Points(GCPs).In fact,space-based high-precision mapping without GCPs is a challenging task that depends on the close cooperation of several payloads and links,of which on-orbit geometric calibration is one of the most critical links.In this paper,the on-orbit geometric calibration of the dual-line array cameras of GF-14 satellite was performed using the control points collected in the high-precision digital calibration field,and the calibration parameters of the dual-line array cameras were solved as a whole by alternate iterations of forward and backward intersection.On this basis,the location accuracy of the stereo images using the calibration parameters was preliminarily evaluated by using several test fields around the world.The evaluation result shows that the direct forward intersection accuracy of GF-14 satellite images without GCPs after on-orbit geometric calibration reaches 2.34 meters(RMS)in plane and 1.97 meters(RMS)in elevation.展开更多
Electroacupuncture has traditionally been used to treat pain, but its effect on pain following brachial plexus injury is still unknown. In this study, rat models of an avulsion injury to the left brachial plexus root(...Electroacupuncture has traditionally been used to treat pain, but its effect on pain following brachial plexus injury is still unknown. In this study, rat models of an avulsion injury to the left brachial plexus root(associated with upper-limb chronic neuropathic pain) were given electroacupuncture stimulation at bilateral Quchi(LI11), Hegu(LI04), Zusanli(ST36) and Yanglingquan(GB34). After electroacupuncture therapy, chronic neuropathic pain in the rats' upper limbs was significantly attenuated. Immunofluorescence staining showed that the expression of β-endorphins in the arcuate nucleus was significantly increased after therapy. Thus, experimental findings indicate that electroacupuncture can attenuate neuropathic pain after brachial plexus injury through upregulating β-endorphin expression.展开更多
Recently, a novel porcine circovirus-like virus P1 with a circular DNA genome of 0.648 kb was identified. P1 antigen was detected both in vitro and in vivo by synthetic peptide-derived polyclonal antibody-based immuno...Recently, a novel porcine circovirus-like virus P1 with a circular DNA genome of 0.648 kb was identified. P1 antigen was detected both in vitro and in vivo by synthetic peptide-derived polyclonal antibody-based immunochemistry. The designed peptides were synthesized by solid-phase technique, purified by high performance liquid chromatography, coupled to Keyhole limpet hemocyanin, and injected into rabbits to prepare polyclonal antibody. The emergence of positive cells revealed that synthetic peptide could elicit antibodies against P1 and viral protein could be synthesized. The polyclonal peptide antibodies described here was successfully applied to immunochemical staining and proved helpful in diagnosing P1.展开更多
基金Independent Project of State Key Laboratory of Geo-information Engineering(SKLGIE2022-ZZ-01)The Youth Science Innovation Fund(No.2023-01)。
文摘GF-14 satellite is a new generation of sub-meter stereo surveying and mapping satellite in China,carrying dual-line array stereo mapping cameras to achieve 1∶10000 scale topographic mapping without Ground Control Points(GCPs).In fact,space-based high-precision mapping without GCPs is a challenging task that depends on the close cooperation of several payloads and links,of which on-orbit geometric calibration is one of the most critical links.In this paper,the on-orbit geometric calibration of the dual-line array cameras of GF-14 satellite was performed using the control points collected in the high-precision digital calibration field,and the calibration parameters of the dual-line array cameras were solved as a whole by alternate iterations of forward and backward intersection.On this basis,the location accuracy of the stereo images using the calibration parameters was preliminarily evaluated by using several test fields around the world.The evaluation result shows that the direct forward intersection accuracy of GF-14 satellite images without GCPs after on-orbit geometric calibration reaches 2.34 meters(RMS)in plane and 1.97 meters(RMS)in elevation.
基金supported by the Project of Ministry of Health(Comprehensive Research on Brachial Plexus Injury),No.13D22270800 from the National Natural Science Foundation of China2011 Shanghai Medical College Young Scientist Fund of Fudan University,No.11L-24
文摘Electroacupuncture has traditionally been used to treat pain, but its effect on pain following brachial plexus injury is still unknown. In this study, rat models of an avulsion injury to the left brachial plexus root(associated with upper-limb chronic neuropathic pain) were given electroacupuncture stimulation at bilateral Quchi(LI11), Hegu(LI04), Zusanli(ST36) and Yanglingquan(GB34). After electroacupuncture therapy, chronic neuropathic pain in the rats' upper limbs was significantly attenuated. Immunofluorescence staining showed that the expression of β-endorphins in the arcuate nucleus was significantly increased after therapy. Thus, experimental findings indicate that electroacupuncture can attenuate neuropathic pain after brachial plexus injury through upregulating β-endorphin expression.
基金Supported by National Natural Science Foundation of China(31272574,30972184)
文摘Recently, a novel porcine circovirus-like virus P1 with a circular DNA genome of 0.648 kb was identified. P1 antigen was detected both in vitro and in vivo by synthetic peptide-derived polyclonal antibody-based immunochemistry. The designed peptides were synthesized by solid-phase technique, purified by high performance liquid chromatography, coupled to Keyhole limpet hemocyanin, and injected into rabbits to prepare polyclonal antibody. The emergence of positive cells revealed that synthetic peptide could elicit antibodies against P1 and viral protein could be synthesized. The polyclonal peptide antibodies described here was successfully applied to immunochemical staining and proved helpful in diagnosing P1.
