Present study was aimed to develop and validate a reverse-phase high-performance liquid chromatography method for simultaneous determination of sitagliptin phosphate and metformin hy-drochloride in a marketed formulat...Present study was aimed to develop and validate a reverse-phase high-performance liquid chromatography method for simultaneous determination of sitagliptin phosphate and metformin hy-drochloride in a marketed formulation. The drug separation was performed on Hibar-240, Li-chrosphere-100 C18 ODS (250 × 4.6 mm, 5 μm) column, at a flow rate of 1 mL/min. The mobile phase used was a mixture of methanol: potassium di-hydrogen phosphate buffer at a ratio of 70:30 v/v. The detection was carried out at a wavelength of 266 nm. The retention times of sitagliptin phosphate and metformin hydrochloride were found as 6.1 and 4.9 min respectively. Linear calibration curves with good correlation coefficients were obtained over the concentration ranges of 10 - 50 μg/mL for sitagliptin and 20 - 100 μg/mL for metformin. The limit of detection was 0.016 and 0.14 μg/mL and the limit of quantification was 0.048 and 0.42 μg/mL for sitagliptin phosphate and metformin hydrochloride respectively. Validation of the method demonstrated system selectivity, specificity, linearity, accuracy and precision. The developed method was found useful in the simultaneous analysis of sitagliptin phosphate and metformin hydrochloride in formulation.展开更多
This paper is devoted to the study of a translation plane π(C) associated with a t-spread set C and its transposed t-spread set C t. In this paper, an explicit matrix form of the inverse of an isomorphism from a tran...This paper is devoted to the study of a translation plane π(C) associated with a t-spread set C and its transposed t-spread set C t. In this paper, an explicit matrix form of the inverse of an isomorphism from a translation plane into another translation plane associated with t-spread sets is derived and proved that two translation planes associated with t-spread sets are isomorphic if and only if their corresponding transposed translation planes are isomorphic. Further, it is shown that the transpose of a flag-transitive plane is flag-transitive and derived a necessary and sufficient condition for a translation plane π(C) to be isomorphic to its transposed translation plane.展开更多
文摘Present study was aimed to develop and validate a reverse-phase high-performance liquid chromatography method for simultaneous determination of sitagliptin phosphate and metformin hy-drochloride in a marketed formulation. The drug separation was performed on Hibar-240, Li-chrosphere-100 C18 ODS (250 × 4.6 mm, 5 μm) column, at a flow rate of 1 mL/min. The mobile phase used was a mixture of methanol: potassium di-hydrogen phosphate buffer at a ratio of 70:30 v/v. The detection was carried out at a wavelength of 266 nm. The retention times of sitagliptin phosphate and metformin hydrochloride were found as 6.1 and 4.9 min respectively. Linear calibration curves with good correlation coefficients were obtained over the concentration ranges of 10 - 50 μg/mL for sitagliptin and 20 - 100 μg/mL for metformin. The limit of detection was 0.016 and 0.14 μg/mL and the limit of quantification was 0.048 and 0.42 μg/mL for sitagliptin phosphate and metformin hydrochloride respectively. Validation of the method demonstrated system selectivity, specificity, linearity, accuracy and precision. The developed method was found useful in the simultaneous analysis of sitagliptin phosphate and metformin hydrochloride in formulation.
文摘This paper is devoted to the study of a translation plane π(C) associated with a t-spread set C and its transposed t-spread set C t. In this paper, an explicit matrix form of the inverse of an isomorphism from a translation plane into another translation plane associated with t-spread sets is derived and proved that two translation planes associated with t-spread sets are isomorphic if and only if their corresponding transposed translation planes are isomorphic. Further, it is shown that the transpose of a flag-transitive plane is flag-transitive and derived a necessary and sufficient condition for a translation plane π(C) to be isomorphic to its transposed translation plane.
