Background:Considering the great heterogeneity of Hepatocellular carcinoma(HCC),more accurate prognostic models are urgently needed.This paper combined the advantages of genomics and pathomics to construct a prognosti...Background:Considering the great heterogeneity of Hepatocellular carcinoma(HCC),more accurate prognostic models are urgently needed.This paper combined the advantages of genomics and pathomics to construct a prognostic model.Methods:First,we collected data from hepatocellular carcinoma patients with complete mRNA expression profiles and clinical annotations from the TCGA database.Then,based on immune-related genes,we used random forest plots to screen prognosis-related genes and build prognostic models.Bioinformatics was used to identify biological pathways,evaluate the tumor microenvironment,and perform drug susceptibility testing.Finally,we divided the patients into different subgroups according to the gene model algorithm.Pathological models were constructed by obtaining HE-stained sections from TCGA in corresponding subgroups of patients.Results:In this study,we constructed a stable prognostic model that could predict overall survival in HCC patients.The signature consisted of six immune-related genes(BX537318.1,TMEM147,CSPG4P12,AC015908.3,CEBPZOS,and SRD5A3).We found increased levels of infiltration of immune cells in the tumor microenvironment in patients with low risk scores,indicating significant antitumor immunity and corresponding to better clinical outcomes.We then screened nine drugs that were more sensitive in the low-risk group than in the high-risk group.Finally,we addressed the complex cellular changes and phenotypic heterogeneity in the HCC microenvironment by combining genomics and pathomics analysis methods.Conclusion:Our study showed that the prognostic evaluation model of HCC based on the immune signaling pathway is feasible and provided a reference value for potential immunotherapy for HCC.展开更多
[Objectives]This study was conducted to explore the effects of using fermented organic fertilizers such as cow manure on the activity of selenium in the soil.[Methods]The project team applied the organic fertilizer pr...[Objectives]This study was conducted to explore the effects of using fermented organic fertilizers such as cow manure on the activity of selenium in the soil.[Methods]The project team applied the organic fertilizer prepared by fermenting cow manure and other raw materials to activate selenium in the soil at two dragon fruit orchard bases in Shilong Town,Guiping City and Dingdang Town,Long an County,and carried out many years of experiments.[Results]The contents of available selenium in the two experimental orchards in Shilong Town and Dingdang Town increased by 10%and 5%,respectively,and the selenium contents in dragon fruit increased by 228.6%and 200.0%,respectively.[Conclusions]The application of fermented organic fertilizers such as cow manure can increase available selenium content in the soil,thereby increasing the selenium content of dragon fruit.展开更多
Objective： The aim of the study was, （1） to observe the short-term efficacy and adverse reactions of icotinib hydrochloride on the treatment of advanced non-small cell lung cancer （NSCLC）; （2） to explore whethe...Objective： The aim of the study was, （1） to observe the short-term efficacy and adverse reactions of icotinib hydrochloride on the treatment of advanced non-small cell lung cancer （NSCLC）; （2） to explore whether there is difference in the efficacy of icotinib hydrochloride among the subgroups of sex, age, smoking history, classification of CEA, histological type, multi-line treatment and PS score. Methods： The study was conducted to collect 138 patients taking icotinib hydrochloride with advanced non-small cell lung cancer in hospitals of Dalian （China） from September 1st 2011 to June 14th 2012. All patients had taken icotinib hydrochloride （125 mg three times a day） until the disease was progressed or the adverse reactions could not be tolerated. During the period of taking it, other anti-tumor treatments were forbidden. We observed the symptoms, such as cough, short breath, hemoptysis, pain. The objective efficacy was evaluated by RECIST criteria, and the adverse reactions related to the treatment was assessed on the basis of NCl-CTC 3.0. Results： Of all patients, CR was 1 （0.7%）, PR was 59 （42.8%）, SD was 37 （26.8%）, PD was 41 （29.7%）. And ORR was 43.5% （60/138）, DCR 70.3% （97/138）. The DCR of females was 83.5% （71/85） versus 49.1% （26/53） of males. The difference of ORR and DCR between the two subgroups had statistical significance （X2 = 8.065, P = 0.05; X2 = 18.577, P = 0.000）. The difference of ORR and DCR between the subgroups of patients after or before 70 years old had no statistical significance. The difference of ORR and DCR between the subgroups of smoking and non-smoking had statistical significance （X2 = 8.492; X2 = 13.602）. The difference of ORR and DCR between the CEA subgroups had statistical significance （X2 = 14.141; X2 = 14.160）, showed 81 patients with abnormal CEA before the treatment with ORR 56.8.0% （46/81）, DCR 81.5% （66/81）; 57 patients of normal CEA before the treatment with ORR 24.6% （14/57）, DCR 52.6% （30/57）. The 36 patients （26.1%） using icotinib hydrochloride as the first-line treatment, 78 patients （56.5%） using icotinib hydrochloride as the second-line, 20 patients （14.5%） using icotinib hydrochloride as the third-line, and 4 patients （2.9%） with tyrosine kinase inhibitor （TKI） resistance, there was statistical difference of DCR among the multi-groups above （~2 = 11.734, P = 0.008）. ORR was 31.1% （14/45） versus DCR 53.3% （24/45） in 45 patients with PS 3-4 points, and ORR was 49.4% （46/93） versus DCR 78.5% （73/93） in 93 patients with PS 0-2 points, and there was statistical difference （X2 = 4.156; X2 = 9.149）. The main adverse reactions were rash （26.8%）, diarrhea （13.8%）, mild liver function abnormal （10.9%）. Conclusion： The short-term efficacy of icotinib hydrochloride on the treatment of advanced NSCLC is positive, and the relevant adverse reactions are mild. The efficacy is better when the patient is female, non-smoker, treated as first-line, with higher CEA before treatment and lower PS scores.展开更多
Objective:To observe the effect of Electroacupuncture of Acupoints of the Neiguan(PC6)and Ximen(PC4)on its myocardial energy metabolism with acute myocardial ischemia rats caused by coronary artery ligation.Methods:Fo...Objective:To observe the effect of Electroacupuncture of Acupoints of the Neiguan(PC6)and Ximen(PC4)on its myocardial energy metabolism with acute myocardial ischemia rats caused by coronary artery ligation.Methods:Forty standard Spargue-Dawely(SD)rats were divided into four groups randomly,namely,group of Neiguan,group of Ximen,model group,sham group.The group of rats of myocardial ischemia were induced by coronary ligation,and the model rat of acute myocardial ischemia was prepared.The sham group had no ligation of the needle.After anesthesia was awakened,acupuncture was applied bilateral at Neiguan and Ximen of the group of Neiguan and Ximen,once a day at a fixed time for 14 days.The rats of model group and the sham group were not given acupunctured,but use the samely methods of Neiguan and Ximen groups to grap the rats at the same time everyday.After 14 days the experiment was over,observed the pathological changes of myocardial tissue by HE staining,detected the levels of free fatty acid(FFA),adenosine triphosphate(ATP)content,and the activities of Na^(+)-K^(+)-ATPase according to the reagents´demand.Results:Pathological results showed that acupuncuted at Neiguan and Ximen could improve the ischemic cardiomyocyte injury.Compared with the sham group,the levels of FFA in model group were increased(p<0.01),the levels of ATP,the activities of Na^(+)-K^(+)-ATPase were all decreased(p<0.01);Compared with the model group,the levels of FFA were decreased(p<0.05,p<0.01),the levels of ATP,the activities of Na^(+)-K^(+)-ATPase were improved.Conclusion:Acupuncturing at the Pericardium Meridian can alleviate the cell injury by myocardial ischemia,and also can improve the myocardial energetic metabolism in acute myocardial ischemia.展开更多
With the sweeping of the age of science and technology,science and technology has developed rapidly in China.As a common monitoring system,drone technology has attracted more attention.Nowadays,with the continuous imp...With the sweeping of the age of science and technology,science and technology has developed rapidly in China.As a common monitoring system,drone technology has attracted more attention.Nowadays,with the continuous improvement of drone technology,campuses have also begun to introduce drone technology for patrol monitoring to ensure students’safety.展开更多
Leucine-rich repeats containing 4(LRRC4,also named netrin-G ligand 2[NGL-2])is a member of the NetrinGs ligands(NGLs)family.As a gene with relatively high and specific expression in brain,it is a member of the leucine...Leucine-rich repeats containing 4(LRRC4,also named netrin-G ligand 2[NGL-2])is a member of the NetrinGs ligands(NGLs)family.As a gene with relatively high and specific expression in brain,it is a member of the leucine-rich repeat superfamily and has been proven to be a suppressor gene for gliomas,thus being involved in gliomagenesis.LRRC4 is the core of microRNA-dependent multi-phase regulatory loops that inhibit the proliferation and invasion of glioblastoma(GB)cells,including LRRC4/NGL2-activator protein 2(AP2)-microRNA(miR)182-LRRC4 and LRRC4-miR185-DNA methyltransferase 1(DNMT1)-LRRC4/specific protein 1(SP1)-DNMT1-LRRC4.In this review,we demonstrated LRRC4 as a new member of the partitioning-defective protein(PAR)polarity complex that promotes axon differentiation,mediates the formation and plasticity of synapses,and assists information input to the hippocampus and storage of memory.As an important synapse regulator,aberrant expression of LRRC4 has been detected in autism,spinal injury and GBs.LRRC4 is a candidate susceptibility gene for autism and a neuro-protective factor in spinal nerve damage.In GBs,LRRC4 is a novel inhibitor of autophagy,and an inhibitor of protein–protein interactions involving in temozolomide resistance,tumor immune microenvironment,and formation of circular RNA.展开更多
What is already known about this topic?After the type 2 strain of the live,attenuated poliovirus vaccine was withdrawn globally in 2016,any identification of a type 2 poliovirus is a Public Health Emergency of Interna...What is already known about this topic?After the type 2 strain of the live,attenuated poliovirus vaccine was withdrawn globally in 2016,any identification of a type 2 poliovirus is a Public Health Emergency of International Concern.A vaccinederived type 2 poliovirus(VDPV2)was identified in Sichuan,prompting an urgent,comprehensive investigation and response.What is added by this report?Type 2 monovalent,live,attenuated oral poliovirus vaccine(mOPV2)is being used to respond to the numerous VDPV2 outbreaks seen around the world.In contrast,the response in Sichuan used Sabin strain inactivated poliovirus(sIPV)to stop circulation of the VDPV2.In the 6 months following the vaccination response,there have been no VDPV2s detected in Sichuan,despite extensive search.What are the implications for public health practices?Further search for the VDPV2 must continue in order to determine whether transmission has been stopped.The ongoing investigation and response to the Sichuan VDPV2 is providing evidence to the Global Polio Eradication Initiative on managing VDPV2 outbreaks.展开更多
文摘Background:Considering the great heterogeneity of Hepatocellular carcinoma(HCC),more accurate prognostic models are urgently needed.This paper combined the advantages of genomics and pathomics to construct a prognostic model.Methods:First,we collected data from hepatocellular carcinoma patients with complete mRNA expression profiles and clinical annotations from the TCGA database.Then,based on immune-related genes,we used random forest plots to screen prognosis-related genes and build prognostic models.Bioinformatics was used to identify biological pathways,evaluate the tumor microenvironment,and perform drug susceptibility testing.Finally,we divided the patients into different subgroups according to the gene model algorithm.Pathological models were constructed by obtaining HE-stained sections from TCGA in corresponding subgroups of patients.Results:In this study,we constructed a stable prognostic model that could predict overall survival in HCC patients.The signature consisted of six immune-related genes(BX537318.1,TMEM147,CSPG4P12,AC015908.3,CEBPZOS,and SRD5A3).We found increased levels of infiltration of immune cells in the tumor microenvironment in patients with low risk scores,indicating significant antitumor immunity and corresponding to better clinical outcomes.We then screened nine drugs that were more sensitive in the low-risk group than in the high-risk group.Finally,we addressed the complex cellular changes and phenotypic heterogeneity in the HCC microenvironment by combining genomics and pathomics analysis methods.Conclusion:Our study showed that the prognostic evaluation model of HCC based on the immune signaling pathway is feasible and provided a reference value for potential immunotherapy for HCC.
基金Nanning Science and Technology Research and Technology Development Key R&D Program(20202087,20212020)Guangxi Science and Technology Major Project(GK AA17202026)+1 种基金Special Project of the Science and Technology Vanguard Special Action of Guangxi Academy of Agricultural Sciences(GNKM 202114)Guangxi Selenium-rich Featured Crops Experimental Station(G TS2016011).
文摘[Objectives]This study was conducted to explore the effects of using fermented organic fertilizers such as cow manure on the activity of selenium in the soil.[Methods]The project team applied the organic fertilizer prepared by fermenting cow manure and other raw materials to activate selenium in the soil at two dragon fruit orchard bases in Shilong Town,Guiping City and Dingdang Town,Long an County,and carried out many years of experiments.[Results]The contents of available selenium in the two experimental orchards in Shilong Town and Dingdang Town increased by 10%and 5%,respectively,and the selenium contents in dragon fruit increased by 228.6%and 200.0%,respectively.[Conclusions]The application of fermented organic fertilizers such as cow manure can increase available selenium content in the soil,thereby increasing the selenium content of dragon fruit.
文摘Objective： The aim of the study was, （1） to observe the short-term efficacy and adverse reactions of icotinib hydrochloride on the treatment of advanced non-small cell lung cancer （NSCLC）; （2） to explore whether there is difference in the efficacy of icotinib hydrochloride among the subgroups of sex, age, smoking history, classification of CEA, histological type, multi-line treatment and PS score. Methods： The study was conducted to collect 138 patients taking icotinib hydrochloride with advanced non-small cell lung cancer in hospitals of Dalian （China） from September 1st 2011 to June 14th 2012. All patients had taken icotinib hydrochloride （125 mg three times a day） until the disease was progressed or the adverse reactions could not be tolerated. During the period of taking it, other anti-tumor treatments were forbidden. We observed the symptoms, such as cough, short breath, hemoptysis, pain. The objective efficacy was evaluated by RECIST criteria, and the adverse reactions related to the treatment was assessed on the basis of NCl-CTC 3.0. Results： Of all patients, CR was 1 （0.7%）, PR was 59 （42.8%）, SD was 37 （26.8%）, PD was 41 （29.7%）. And ORR was 43.5% （60/138）, DCR 70.3% （97/138）. The DCR of females was 83.5% （71/85） versus 49.1% （26/53） of males. The difference of ORR and DCR between the two subgroups had statistical significance （X2 = 8.065, P = 0.05; X2 = 18.577, P = 0.000）. The difference of ORR and DCR between the subgroups of patients after or before 70 years old had no statistical significance. The difference of ORR and DCR between the subgroups of smoking and non-smoking had statistical significance （X2 = 8.492; X2 = 13.602）. The difference of ORR and DCR between the CEA subgroups had statistical significance （X2 = 14.141; X2 = 14.160）, showed 81 patients with abnormal CEA before the treatment with ORR 56.8.0% （46/81）, DCR 81.5% （66/81）; 57 patients of normal CEA before the treatment with ORR 24.6% （14/57）, DCR 52.6% （30/57）. The 36 patients （26.1%） using icotinib hydrochloride as the first-line treatment, 78 patients （56.5%） using icotinib hydrochloride as the second-line, 20 patients （14.5%） using icotinib hydrochloride as the third-line, and 4 patients （2.9%） with tyrosine kinase inhibitor （TKI） resistance, there was statistical difference of DCR among the multi-groups above （~2 = 11.734, P = 0.008）. ORR was 31.1% （14/45） versus DCR 53.3% （24/45） in 45 patients with PS 3-4 points, and ORR was 49.4% （46/93） versus DCR 78.5% （73/93） in 93 patients with PS 0-2 points, and there was statistical difference （X2 = 4.156; X2 = 9.149）. The main adverse reactions were rash （26.8%）, diarrhea （13.8%）, mild liver function abnormal （10.9%）. Conclusion： The short-term efficacy of icotinib hydrochloride on the treatment of advanced NSCLC is positive, and the relevant adverse reactions are mild. The efficacy is better when the patient is female, non-smoker, treated as first-line, with higher CEA before treatment and lower PS scores.
基金Anhui University of Traditional Chinese Medicine General Project of School-level Natural Science Research in 2019(No.2019zryb14)。
文摘Objective:To observe the effect of Electroacupuncture of Acupoints of the Neiguan(PC6)and Ximen(PC4)on its myocardial energy metabolism with acute myocardial ischemia rats caused by coronary artery ligation.Methods:Forty standard Spargue-Dawely(SD)rats were divided into four groups randomly,namely,group of Neiguan,group of Ximen,model group,sham group.The group of rats of myocardial ischemia were induced by coronary ligation,and the model rat of acute myocardial ischemia was prepared.The sham group had no ligation of the needle.After anesthesia was awakened,acupuncture was applied bilateral at Neiguan and Ximen of the group of Neiguan and Ximen,once a day at a fixed time for 14 days.The rats of model group and the sham group were not given acupunctured,but use the samely methods of Neiguan and Ximen groups to grap the rats at the same time everyday.After 14 days the experiment was over,observed the pathological changes of myocardial tissue by HE staining,detected the levels of free fatty acid(FFA),adenosine triphosphate(ATP)content,and the activities of Na^(+)-K^(+)-ATPase according to the reagents´demand.Results:Pathological results showed that acupuncuted at Neiguan and Ximen could improve the ischemic cardiomyocyte injury.Compared with the sham group,the levels of FFA in model group were increased(p<0.01),the levels of ATP,the activities of Na^(+)-K^(+)-ATPase were all decreased(p<0.01);Compared with the model group,the levels of FFA were decreased(p<0.05,p<0.01),the levels of ATP,the activities of Na^(+)-K^(+)-ATPase were improved.Conclusion:Acupuncturing at the Pericardium Meridian can alleviate the cell injury by myocardial ischemia,and also can improve the myocardial energetic metabolism in acute myocardial ischemia.
基金Jiaxing University Doctoral Fund(CD70519047)Natural Science Foundation of Zhejiang Province(No.LY18F020020)+1 种基金Humanity and Social Science Youth Foundation of Ministry of Education of China(No.17YJCZH033)Zhejiang Provincial Educational Science Planning Topic of China(No.GH2020035).
文摘With the sweeping of the age of science and technology,science and technology has developed rapidly in China.As a common monitoring system,drone technology has attracted more attention.Nowadays,with the continuous improvement of drone technology,campuses have also begun to introduce drone technology for patrol monitoring to ensure students’safety.
基金National Natural Science Foundation of China(No.82073096)Hunan Provincial Natural Science Foundation of China(No.2022JJ40578)
文摘Leucine-rich repeats containing 4(LRRC4,also named netrin-G ligand 2[NGL-2])is a member of the NetrinGs ligands(NGLs)family.As a gene with relatively high and specific expression in brain,it is a member of the leucine-rich repeat superfamily and has been proven to be a suppressor gene for gliomas,thus being involved in gliomagenesis.LRRC4 is the core of microRNA-dependent multi-phase regulatory loops that inhibit the proliferation and invasion of glioblastoma(GB)cells,including LRRC4/NGL2-activator protein 2(AP2)-microRNA(miR)182-LRRC4 and LRRC4-miR185-DNA methyltransferase 1(DNMT1)-LRRC4/specific protein 1(SP1)-DNMT1-LRRC4.In this review,we demonstrated LRRC4 as a new member of the partitioning-defective protein(PAR)polarity complex that promotes axon differentiation,mediates the formation and plasticity of synapses,and assists information input to the hippocampus and storage of memory.As an important synapse regulator,aberrant expression of LRRC4 has been detected in autism,spinal injury and GBs.LRRC4 is a candidate susceptibility gene for autism and a neuro-protective factor in spinal nerve damage.In GBs,LRRC4 is a novel inhibitor of autophagy,and an inhibitor of protein–protein interactions involving in temozolomide resistance,tumor immune microenvironment,and formation of circular RNA.
基金supported by National Science and Technology Major Project of China(2018ZX10101002-003).
文摘What is already known about this topic?After the type 2 strain of the live,attenuated poliovirus vaccine was withdrawn globally in 2016,any identification of a type 2 poliovirus is a Public Health Emergency of International Concern.A vaccinederived type 2 poliovirus(VDPV2)was identified in Sichuan,prompting an urgent,comprehensive investigation and response.What is added by this report?Type 2 monovalent,live,attenuated oral poliovirus vaccine(mOPV2)is being used to respond to the numerous VDPV2 outbreaks seen around the world.In contrast,the response in Sichuan used Sabin strain inactivated poliovirus(sIPV)to stop circulation of the VDPV2.In the 6 months following the vaccination response,there have been no VDPV2s detected in Sichuan,despite extensive search.What are the implications for public health practices?Further search for the VDPV2 must continue in order to determine whether transmission has been stopped.The ongoing investigation and response to the Sichuan VDPV2 is providing evidence to the Global Polio Eradication Initiative on managing VDPV2 outbreaks.
