Histone acetylation is involved in chromatin structural remodeling and the regulation of gene expression, and it is important for the activation of defense-related gene expression in eukaryotes[1]. The silent informat...Histone acetylation is involved in chromatin structural remodeling and the regulation of gene expression, and it is important for the activation of defense-related gene expression in eukaryotes[1]. The silent information regulator 2(SIR2) family of proteins were initially described as nuclear proteins with histone deacetylase activity that causes chromatin compaction and gene silencing[2].展开更多
Virus-derived small interference RNAs(vsiRNAs)not only suppress virus infection in plants via induction of RNA silencing but also enhance virus infection by regulating host defensive gene expression.However,the underl...Virus-derived small interference RNAs(vsiRNAs)not only suppress virus infection in plants via induction of RNA silencing but also enhance virus infection by regulating host defensive gene expression.However,the underlying mechanisms that control vsiRNA-mediated host immunity or susceptibility remain largely unknown.In this study,we generated several transgenic wheat lines using four artificial microRNA expression vectors carrying vsiRNAs from Wheat yellow mosaic virus(WYMV)RNA1.Laboratory and field tests showed that two transgenic wheat lines expressing amiRNAI were highly resistant to WYMV infection.Further analyses showed that vsiRNAI could modulate the expression of a wheat thioredoxin-like gene(TaAAEDI),which encodes a negative regulator of reactive oxygen species(ROS)production in the chloroplast.The function of TaAAEDI in ROS scavenging could be suppressed by vsiRNAI in a dose-dependent manner.Furthermore,transgenic expression of amiRNAI in wheat resulted in broad-spectrum disease resistance to Chinese wheat mosaic virus,Barley stripe mosaic virus,and Puccinia striiformis f.sp.tritici infection,suggesting that vsiRNAI is involved in wheat immunity via ROS signaling.Collectively,these findings reveal a previously unidentified mechanism underlying the arms race between viruses and plants.展开更多
基金supported by the National Key Research and Development Program of China(2023YFD1400300)Zhejiang Provincial Natural Science Foundation of China(LY23C140006)+2 种基金National Natural Science Foundation of China(31901954)Ningbo Natural Science Foundation(2021J126,2023J382)China Agriculture Research System of MOF and MARA(CARS-03)。
文摘Histone acetylation is involved in chromatin structural remodeling and the regulation of gene expression, and it is important for the activation of defense-related gene expression in eukaryotes[1]. The silent information regulator 2(SIR2) family of proteins were initially described as nuclear proteins with histone deacetylase activity that causes chromatin compaction and gene silencing[2].
基金the National Key R&D Plan in China,China(2017YFD-0201701)National Key Project for Research on Transgenic Biology,China(2016ZX08002-001)+3 种基金Natural Science Foundation of Zhejiang Province,China(LQ20C140002)Ningbo Science and Technology Innovation 2025 Major Project,China(Q21C140013)Natural Science Foundation of Ningbo City,China(2019A610415,2019A610410)China Agricultural Research System from the Ministry of Agriculture,China(CARS-03)。
文摘Virus-derived small interference RNAs(vsiRNAs)not only suppress virus infection in plants via induction of RNA silencing but also enhance virus infection by regulating host defensive gene expression.However,the underlying mechanisms that control vsiRNA-mediated host immunity or susceptibility remain largely unknown.In this study,we generated several transgenic wheat lines using four artificial microRNA expression vectors carrying vsiRNAs from Wheat yellow mosaic virus(WYMV)RNA1.Laboratory and field tests showed that two transgenic wheat lines expressing amiRNAI were highly resistant to WYMV infection.Further analyses showed that vsiRNAI could modulate the expression of a wheat thioredoxin-like gene(TaAAEDI),which encodes a negative regulator of reactive oxygen species(ROS)production in the chloroplast.The function of TaAAEDI in ROS scavenging could be suppressed by vsiRNAI in a dose-dependent manner.Furthermore,transgenic expression of amiRNAI in wheat resulted in broad-spectrum disease resistance to Chinese wheat mosaic virus,Barley stripe mosaic virus,and Puccinia striiformis f.sp.tritici infection,suggesting that vsiRNAI is involved in wheat immunity via ROS signaling.Collectively,these findings reveal a previously unidentified mechanism underlying the arms race between viruses and plants.
