The current study aims to investigate a suitable adhesive for primary tooth enamel. Shear bond strength(SBS)of primary teeth and the length of resin protrusion were analyzed using one-way ANOVA with Bonferroni multipl...The current study aims to investigate a suitable adhesive for primary tooth enamel. Shear bond strength(SBS)of primary teeth and the length of resin protrusion were analyzed using one-way ANOVA with Bonferroni multiple comparison tests after etching with 35% H_(3)PO_(4). SBS and marginal microleakage tests were conducted with Single Bond Universal(SBU)/Single Bond 2(SB2) adhesives with or without pre-etching using a nonparametric Kruskal-Wallis test. Clinical investigations were performed to validate the adhesive for primary teeth restoration using Chi-square tests. Results showed that the SBS and length of resin protrusion increased significantly with the etching time. Teeth in the SBU with 35% H_(3)PO_(4)pre-etching groups had higher bond strength and lower marginal microleakage than those in the SB2 groups. Mixed fractures were more common in the 35% H_(3)PO_(4)etched 30 s + SB2/SBU groups. Clinical investigations showed significant differences between the two groups in cumulative retention rates at the 6-, 12-and 18-month follow-up evaluations, as well as in marginal adaptation, discoloration, and secondary caries at the 12-and 18-month follow-up assessments.Together, pre-etching primary teeth enamel for 30 s before SBU treatment improved clinical composite resin restoration, which can provide a suitable approach for restoration of primary teeth.展开更多
Certain pseudogenes may regulate their protein-coding cousins by competing for miRNAs and play an active biological role in cancer.However,few studies have focused on the association of genetic variations in pseudogen...Certain pseudogenes may regulate their protein-coding cousins by competing for miRNAs and play an active biological role in cancer.However,few studies have focused on the association of genetic variations in pseudogenes with cancer prognosis.We selected six potentially functional single nucleotide polymorphisms(SNPs) in cancerrelated pseudogenes,and performed a case-only study to assess the association between those SNPs and the prognosis of hepatocellular carcinoma(HCC) in 331 HBV-positive HCC patients without surgical treatment.Log-rank test and Cox proportional hazard models were used for survival analysis.We found that the A allele of rs9909601 in E2F3P1was significantly associated with a better prognosis compared with the G allele[adjusted hazard ratio(HR) = 0.69,95%confidence interval(CI) = 0.56-0.86,P = 0.001].Additionally,this protective effect was more predominant for patients without chemotherapy and transcatheter hepatic arterial chemoembolization(TACE) treatment.Interestingly,we also detected a statistically significant multiplicative interaction between genotypes of rs9909601 and chemotherapy or TACE status on HCC survival(P for multiplicative interaction < 0.001).These findings indicate that rs9909601 in the pseudogene E2F3P1 may be a genetic marker for HCC prognosis in Chinese.展开更多
Recent studies showed that pseudogenes can regulate the expression of their coding gene partners by competing for miRNAs. The E2F family plays a crucial role in the control of cell cycle checkpoint. E2F3P1 is a pseudo...Recent studies showed that pseudogenes can regulate the expression of their coding gene partners by competing for miRNAs. The E2F family plays a crucial role in the control of cell cycle checkpoint. E2F3P1 is a pseudogene of E2F3. Few studies focused on genetic variations on pseudogenes. In this study, we performed a case-control study to assess the association between single nucleotide polymorphisms (SNPs) in E2F3P1 and hepatocellular carcinoma (HCC) risk in 1050 hepatitis B virus (HBV)-positive HCC cases and 1050 chronic HBV carriers. Logistic regression analysis was applied to estimate odds ratios (ORs) and 95% confidence intervals (CIs) for the associations between genotypes and HCC risk. We found that the variant CT/TT genotypes of rs1838149 were associated with a significantly decreased risk of HCC (adjusted OR=0.66, 95% CIs=0.51-0.86, P=0.002) compared to those with wildtype CC homozygote. Furthermore, the AA genotype of rs9909601 had an increased HCC risk with an adjusted OR of 1.41 (95% CIs=1.07-1.86), and the A allele of rs9909601 was significantly associated with HCC risk compared to those with the G allele (adjusted OR=1.17, 95% CIs=1.03-1.33, P=0.017). These results indicate that genetic variations in the pseudogene E2F3P1 may confer HCC risk.展开更多
基金supported by the Technology Development Fund of Nanjing Medical University(Grants No.NMUB2016115 and NMUB2020117)。
文摘The current study aims to investigate a suitable adhesive for primary tooth enamel. Shear bond strength(SBS)of primary teeth and the length of resin protrusion were analyzed using one-way ANOVA with Bonferroni multiple comparison tests after etching with 35% H_(3)PO_(4). SBS and marginal microleakage tests were conducted with Single Bond Universal(SBU)/Single Bond 2(SB2) adhesives with or without pre-etching using a nonparametric Kruskal-Wallis test. Clinical investigations were performed to validate the adhesive for primary teeth restoration using Chi-square tests. Results showed that the SBS and length of resin protrusion increased significantly with the etching time. Teeth in the SBU with 35% H_(3)PO_(4)pre-etching groups had higher bond strength and lower marginal microleakage than those in the SB2 groups. Mixed fractures were more common in the 35% H_(3)PO_(4)etched 30 s + SB2/SBU groups. Clinical investigations showed significant differences between the two groups in cumulative retention rates at the 6-, 12-and 18-month follow-up evaluations, as well as in marginal adaptation, discoloration, and secondary caries at the 12-and 18-month follow-up assessments.Together, pre-etching primary teeth enamel for 30 s before SBU treatment improved clinical composite resin restoration, which can provide a suitable approach for restoration of primary teeth.
基金funded by the National Natural Science Foundation of China(81372606 and 81072344)supported by the National Key Basic Research Program Grant(2013CB911400)+6 种基金the project supportedby the National Science Foundation for Distinguished Young Scholarsof China(81225020)Foundation of Jiangsu Province for Distinguished Young Scholars(BK2012042)Foundation for the Program for NewCentury Excellent Talents in University(NCET-10-0178)the Fok Ying-Tong Education Foundation for Young Teachers in the Higher Education Institutions(122031)Young Tip-top Talents Support Program by the Organization Department of the CPC Central Committee,the Author of National Excellent Doctoral Dissertation(201081)Jiangsu Province Clinical Science and Technology Projects(BL2012008)the Priority Academic Program for the Development of Jiangsu Higher Education Institutions(Public Health and PreventiveMedicine)
文摘Certain pseudogenes may regulate their protein-coding cousins by competing for miRNAs and play an active biological role in cancer.However,few studies have focused on the association of genetic variations in pseudogenes with cancer prognosis.We selected six potentially functional single nucleotide polymorphisms(SNPs) in cancerrelated pseudogenes,and performed a case-only study to assess the association between those SNPs and the prognosis of hepatocellular carcinoma(HCC) in 331 HBV-positive HCC patients without surgical treatment.Log-rank test and Cox proportional hazard models were used for survival analysis.We found that the A allele of rs9909601 in E2F3P1was significantly associated with a better prognosis compared with the G allele[adjusted hazard ratio(HR) = 0.69,95%confidence interval(CI) = 0.56-0.86,P = 0.001].Additionally,this protective effect was more predominant for patients without chemotherapy and transcatheter hepatic arterial chemoembolization(TACE) treatment.Interestingly,we also detected a statistically significant multiplicative interaction between genotypes of rs9909601 and chemotherapy or TACE status on HCC survival(P for multiplicative interaction < 0.001).These findings indicate that rs9909601 in the pseudogene E2F3P1 may be a genetic marker for HCC prognosis in Chinese.
基金funded by the National Key Basic Research Program (2013CB911400)the Foundation for the Program for New Century Excellent Talents in University (NCET-10-0178)+5 种基金the Author of National Excellent Doctoral Dissertation (201081)the National Natural Science Foundation of China (30800946 and 81072344)the State Key Infectious Disease Project of China (2012ZX10002010, 2012ZX10002016)the National Major S&T Projects 2011ZX10004902)the National Science Fund for Creative Research Groups (30921006)the Priority Academic Program for the Development of Jiangsu Higher Education Institutions (Public Health and Preventive Medicine)
文摘Recent studies showed that pseudogenes can regulate the expression of their coding gene partners by competing for miRNAs. The E2F family plays a crucial role in the control of cell cycle checkpoint. E2F3P1 is a pseudogene of E2F3. Few studies focused on genetic variations on pseudogenes. In this study, we performed a case-control study to assess the association between single nucleotide polymorphisms (SNPs) in E2F3P1 and hepatocellular carcinoma (HCC) risk in 1050 hepatitis B virus (HBV)-positive HCC cases and 1050 chronic HBV carriers. Logistic regression analysis was applied to estimate odds ratios (ORs) and 95% confidence intervals (CIs) for the associations between genotypes and HCC risk. We found that the variant CT/TT genotypes of rs1838149 were associated with a significantly decreased risk of HCC (adjusted OR=0.66, 95% CIs=0.51-0.86, P=0.002) compared to those with wildtype CC homozygote. Furthermore, the AA genotype of rs9909601 had an increased HCC risk with an adjusted OR of 1.41 (95% CIs=1.07-1.86), and the A allele of rs9909601 was significantly associated with HCC risk compared to those with the G allele (adjusted OR=1.17, 95% CIs=1.03-1.33, P=0.017). These results indicate that genetic variations in the pseudogene E2F3P1 may confer HCC risk.
