Laser irradiation device for the protection of biological objects from the action of ionizing radiation to be used in practice has been manufactured （invention patent RU 2 428 228 C2）. Research of the action of y-ra...Laser irradiation device for the protection of biological objects from the action of ionizing radiation to be used in practice has been manufactured （invention patent RU 2 428 228 C2）. Research of the action of y-radiation itself as well as of the combined action of laser devices on survival, weight, skin and the general mitotic index of the bone marrow cells （mitotic index of all nucleus-containing cells of the bone marrow） of C57BL/6 experimental young mice was carried out. The mice were irradiated with ionizing （whole body irradiation） and laser radiation, separately one by one in a special frame device. Laser radiation in the dose 1 mJ/cm^2 irradiated only the back of a mouse, or both the back and the abdomen of mice. In case of combined irradiation of mice, the time interval between two types of irradiation did not exceed 30 min. First, the mice were exposed to y-radiation then to laser radiation. The method of the laser radiation-protection of biological subjects contributes to an increase in the viability of mice, prevents the damages of skin and also increases the mitotic activity of mice bone marrow cells.展开更多
An opportunity to use laser radiation as a means to reduce negative aftermath of acute and prolonged exposure to ionizing radiation was checked. The mice were exposed to γ- rays of ^60Co （whole body irradiation） in...An opportunity to use laser radiation as a means to reduce negative aftermath of acute and prolonged exposure to ionizing radiation was checked. The mice were exposed to γ- rays of ^60Co （whole body irradiation） in the dose of 7 Gy （the transitional clinical form of the acute radiation sickness）. The dose rate at acute irradiation was 1.14 Gy/min, and at prolonged exposure, 0.027 Gy/min. Laser radiation in the dose l mJ/cm^2 was used to irradiate only the back of a mouse. First, the mice were exposed to γ-radiation, then to laser radiation. The time interval between two types of irradiation did not exceed 30 min. It was shown that the radiation protection of mice with laser radiation is possible at exposure to ionizing radiation in a wide dose interval and can reduce negative after-effects of both the acute and prolonged radiation exposure.展开更多
Studies have been carried out to test the assumption that the primary photoreceptors for the radioprotective action of 633 nm laser radiation are the cytochrome-c-oxidases.To do this,the device was created for radiati...Studies have been carried out to test the assumption that the primary photoreceptors for the radioprotective action of 633 nm laser radiation are the cytochrome-c-oxidases.To do this,the device was created for radiation protection of biological objects based on laser module with a wavelength of 532 nm.Experiments conducted on murine fibroblast cells showed that the radioprotection effect of laser irradiation was observed in the dose interval about 0.4-0.85 mJ/cm2.Maximum radioprotection effect is observed at laser radiation energy density^0.56 mJ/cm2.The determination of the cell survival with the automatic counterСТ20 after the action of the ionizing and combined irradiation showed that radioprotective action of the laser radiation with the wavelength of 533 nm,as well as the radiation with the wavelength of 633 nm,is transferred by the mechanism of the“bystander effect”.In addition,it was found that radioprotection effect of laser irradiation observed on the criterion of number of surviving single cells,compared with cells exposed to?-radiation Laser irradiation,produces effective radioprotecting action also on the criterion of grown cell colonies.The value of the dose modification factor(DMF)calculated on 50%cell survival(LD50)is equal to 1.4.The results suggest that in the case of radioprotective action of small doses of laser radiation with a wavelength of 633 nm,as well as 532 nm primary photoreceptors is the cytochrome-c-oxidases.展开更多
A research was carried out to determine the period of time during which it is possible to reduce the radiation damage in mice by means of laser radiation （650 nm） after gamma irradiation. First, the mice were expose...A research was carried out to determine the period of time during which it is possible to reduce the radiation damage in mice by means of laser radiation （650 nm） after gamma irradiation. First, the mice were exposed to γ- radiation （whole body irradiation）, then after 2 h or 24 h they were irradiated with laser radiation. The results of these studies have shown that the use of laser irradiation to reduce radiation damage in mice is effective 24 h after the exposure to 5 Gy ionizing radiation which leads to the bone-marrow clinical form of the ARS （Acute radiation sickness）. In case of the lethal dose of ionizing radiation 7 Gy （the transitional clinical form of the ARS）, the increase in life expectancy of mice is observed using laser radiation both 2 and 24 h after the exposure to γ- radiation, but the effectiveness of the laser used 2 h after the ionizing radiation is significantly more efficient.展开更多
文摘Laser irradiation device for the protection of biological objects from the action of ionizing radiation to be used in practice has been manufactured （invention patent RU 2 428 228 C2）. Research of the action of y-radiation itself as well as of the combined action of laser devices on survival, weight, skin and the general mitotic index of the bone marrow cells （mitotic index of all nucleus-containing cells of the bone marrow） of C57BL/6 experimental young mice was carried out. The mice were irradiated with ionizing （whole body irradiation） and laser radiation, separately one by one in a special frame device. Laser radiation in the dose 1 mJ/cm^2 irradiated only the back of a mouse, or both the back and the abdomen of mice. In case of combined irradiation of mice, the time interval between two types of irradiation did not exceed 30 min. First, the mice were exposed to y-radiation then to laser radiation. The method of the laser radiation-protection of biological subjects contributes to an increase in the viability of mice, prevents the damages of skin and also increases the mitotic activity of mice bone marrow cells.
文摘An opportunity to use laser radiation as a means to reduce negative aftermath of acute and prolonged exposure to ionizing radiation was checked. The mice were exposed to γ- rays of ^60Co （whole body irradiation） in the dose of 7 Gy （the transitional clinical form of the acute radiation sickness）. The dose rate at acute irradiation was 1.14 Gy/min, and at prolonged exposure, 0.027 Gy/min. Laser radiation in the dose l mJ/cm^2 was used to irradiate only the back of a mouse. First, the mice were exposed to γ-radiation, then to laser radiation. The time interval between two types of irradiation did not exceed 30 min. It was shown that the radiation protection of mice with laser radiation is possible at exposure to ionizing radiation in a wide dose interval and can reduce negative after-effects of both the acute and prolonged radiation exposure.
文摘Studies have been carried out to test the assumption that the primary photoreceptors for the radioprotective action of 633 nm laser radiation are the cytochrome-c-oxidases.To do this,the device was created for radiation protection of biological objects based on laser module with a wavelength of 532 nm.Experiments conducted on murine fibroblast cells showed that the radioprotection effect of laser irradiation was observed in the dose interval about 0.4-0.85 mJ/cm2.Maximum radioprotection effect is observed at laser radiation energy density^0.56 mJ/cm2.The determination of the cell survival with the automatic counterСТ20 after the action of the ionizing and combined irradiation showed that radioprotective action of the laser radiation with the wavelength of 533 nm,as well as the radiation with the wavelength of 633 nm,is transferred by the mechanism of the“bystander effect”.In addition,it was found that radioprotection effect of laser irradiation observed on the criterion of number of surviving single cells,compared with cells exposed to?-radiation Laser irradiation,produces effective radioprotecting action also on the criterion of grown cell colonies.The value of the dose modification factor(DMF)calculated on 50%cell survival(LD50)is equal to 1.4.The results suggest that in the case of radioprotective action of small doses of laser radiation with a wavelength of 633 nm,as well as 532 nm primary photoreceptors is the cytochrome-c-oxidases.
文摘A research was carried out to determine the period of time during which it is possible to reduce the radiation damage in mice by means of laser radiation （650 nm） after gamma irradiation. First, the mice were exposed to γ- radiation （whole body irradiation）, then after 2 h or 24 h they were irradiated with laser radiation. The results of these studies have shown that the use of laser irradiation to reduce radiation damage in mice is effective 24 h after the exposure to 5 Gy ionizing radiation which leads to the bone-marrow clinical form of the ARS （Acute radiation sickness）. In case of the lethal dose of ionizing radiation 7 Gy （the transitional clinical form of the ARS）, the increase in life expectancy of mice is observed using laser radiation both 2 and 24 h after the exposure to γ- radiation, but the effectiveness of the laser used 2 h after the ionizing radiation is significantly more efficient.
