Reversed-phase high-performance liquid chromatography （RP-HPLC） method with C4 column and C18 column for analyzing β-lactoglobulin and α-lactalbumin in bovine milk was developed and the performance and characteris...Reversed-phase high-performance liquid chromatography （RP-HPLC） method with C4 column and C18 column for analyzing β-lactoglobulin and α-lactalbumin in bovine milk was developed and the performance and characteristic of two columns were compared. Shiseido Proteonavi C4 column （250 mm×4.6 mm×5μm） and Shiseido CAPCELL PAK SG 300 C18 column （250 mm× 4.6 mm×5 μm） were used in the experiment. Phase A was composed of 0.1% （V/V） trifluoroacetic acid （TFA） in ultrapure water and Phase B （organic phase） was composed of 0.1% TFA in acetonitrile. Gradient elution was taken. Flow rate was 1 mL min-1. The detection wavelength was 215 nm. The injection volume was 20 μL and the column temperature was 30℃. The results showed that linear relationship was good and recovery of α-lactalbumin and β-lactoglobulin was 86.12%-104.38%, C18 column had stronger ability to resist acid and more stable, and the method with C4 column had excellent sensitivities and good separation.展开更多
斑鲆属(Pseudorhombus,Bleeker,1862)隶属于鲽形目(Pleuronectiforms)牙鲆科(Paralichthyidae),根据Eschmeyer’s Catalog of Fishes记录有效种类有25种;根据李思忠等(1995)的《中国动物志:硬骨鱼纲鲽形目》记载,目前在中国大陆海域有...斑鲆属(Pseudorhombus,Bleeker,1862)隶属于鲽形目(Pleuronectiforms)牙鲆科(Paralichthyidae),根据Eschmeyer’s Catalog of Fishes记录有效种类有25种;根据李思忠等(1995)的《中国动物志:硬骨鱼纲鲽形目》记载,目前在中国大陆海域有标本记录的有11种。本研究在2007年到2021年在海南的文昌、陵水和三亚分别采集到了9尾有眼侧有5个眼状斑纹的标本,并和该属25种鱼类的原始形态特征进行了比较。比对结果发现,该样品符合Amaoka(1969)定名的卷眼斑鲆(P.oculocirris Amaoka,1969)形态特征。该种类目前只在日本和越南有报道,本研究是首次在中国大陆海域采集,故为新记录种。本研究结合卷眼斑鲆原始描述、相关文献和采集到的9尾标本的形态特征对该种类进行了详细的再描述,并首次提供了线粒体分子条形码(GenBank登录号为OL307681、OL307682和OL307683)。该种的主要形态特征为背鳍65–76;臀鳍50–57;有眼侧胸鳍11–13,无眼侧胸鳍10–12;尾鳍条2+13+2;第一鳃弓鳃耙4–7+17–20;侧线鳞71–82;脊椎骨10+26=36;背鳍前边几个鳍条稍长且基部只有1/5–1/3连有鳍膜,下眼上缘具有指状皮突。本研究的发现进一步扩大了该种的分布范围和提供了种类鉴定的分子依据,本报道将会为牙鲆科鱼类生物多样性和系统演化研究提供科学依据。展开更多
基金Supported by the Project of Science & Technology Plans in Heilongjiang Province in the 11th Five-year Period (GB07B407)
文摘Reversed-phase high-performance liquid chromatography （RP-HPLC） method with C4 column and C18 column for analyzing β-lactoglobulin and α-lactalbumin in bovine milk was developed and the performance and characteristic of two columns were compared. Shiseido Proteonavi C4 column （250 mm×4.6 mm×5μm） and Shiseido CAPCELL PAK SG 300 C18 column （250 mm× 4.6 mm×5 μm） were used in the experiment. Phase A was composed of 0.1% （V/V） trifluoroacetic acid （TFA） in ultrapure water and Phase B （organic phase） was composed of 0.1% TFA in acetonitrile. Gradient elution was taken. Flow rate was 1 mL min-1. The detection wavelength was 215 nm. The injection volume was 20 μL and the column temperature was 30℃. The results showed that linear relationship was good and recovery of α-lactalbumin and β-lactoglobulin was 86.12%-104.38%, C18 column had stronger ability to resist acid and more stable, and the method with C4 column had excellent sensitivities and good separation.
文摘斑鲆属(Pseudorhombus,Bleeker,1862)隶属于鲽形目(Pleuronectiforms)牙鲆科(Paralichthyidae),根据Eschmeyer’s Catalog of Fishes记录有效种类有25种;根据李思忠等(1995)的《中国动物志:硬骨鱼纲鲽形目》记载,目前在中国大陆海域有标本记录的有11种。本研究在2007年到2021年在海南的文昌、陵水和三亚分别采集到了9尾有眼侧有5个眼状斑纹的标本,并和该属25种鱼类的原始形态特征进行了比较。比对结果发现,该样品符合Amaoka(1969)定名的卷眼斑鲆(P.oculocirris Amaoka,1969)形态特征。该种类目前只在日本和越南有报道,本研究是首次在中国大陆海域采集,故为新记录种。本研究结合卷眼斑鲆原始描述、相关文献和采集到的9尾标本的形态特征对该种类进行了详细的再描述,并首次提供了线粒体分子条形码(GenBank登录号为OL307681、OL307682和OL307683)。该种的主要形态特征为背鳍65–76;臀鳍50–57;有眼侧胸鳍11–13,无眼侧胸鳍10–12;尾鳍条2+13+2;第一鳃弓鳃耙4–7+17–20;侧线鳞71–82;脊椎骨10+26=36;背鳍前边几个鳍条稍长且基部只有1/5–1/3连有鳍膜,下眼上缘具有指状皮突。本研究的发现进一步扩大了该种的分布范围和提供了种类鉴定的分子依据,本报道将会为牙鲆科鱼类生物多样性和系统演化研究提供科学依据。
