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Antibody Titer of Newcastle Disease in Vaccinated and Non-vaccinated Local Chicken of Cambodia
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作者 Sokha Thim Solida Keo +4 位作者 Kannan Dim Kroesna Kang Sath Keo Vutey Venn kouch theng 《Journal of Environmental Science and Engineering(B)》 CAS 2024年第2期80-85,共6页
The experiment was conducted at Veterinary Research Station of Faculty of Veterinary Medicine,Royal University of Agriculture.The experimental period lasted 60 days,starting from October 1st to November 30th 2022.CRD(... The experiment was conducted at Veterinary Research Station of Faculty of Veterinary Medicine,Royal University of Agriculture.The experimental period lasted 60 days,starting from October 1st to November 30th 2022.CRD(Completely Randomized Design)was used with 2 treatments/groups,vaccination group and non-vaccination group“control”,and 6 replications.The vaccination groups received two times of vaccination by dropping into the ocular at 7 days and 21 days.Meanwhile,blood samples were collected 3 times to detect the antibody level of ND(Newcastle Disease)and contained 21 days old,35 days old and 49 days old chicks.The ELISA(Enzyme-Linked Immunosorbent Assay)was performed to detect the antibody of those 2 groups.The result of finding showed that the S/P(Sample to Positive)ratio of control at 21 days,was very low,even in 3rd quartile,which was below the threshold.However,the vaccination group was relatively high,even in 1st quartile,which was higher than the threshold.At 35 days,S/P ratio of control group was still very low,but a bit higher than at 21 days.Meanwhile,the vaccination group was still high,even in 1st quartile,and two-time higher than at 21 days,but an increasing number of samples developed less antibody than threshold,accounting for 12.22%.At 49 days,the control group was still very low,even in 3rd quartile,but a bit higher than at 21 days and 35 days,and was close to the threshold.The vaccination group was still relatively high,even in 1st quartile but lower than three times comparing to 35 days.However,in this age,the number of chickens that developed antibody seemed to be increased in the control group,vice versa for vaccination group.The average S/P ratio was different significant(p<0.001),where vaccination had higher S/P ratio than control.It was similar finding for log-titer,the vaccination had higher figure(p<0.001).The risk of infection of ND was higher in control group,but it will reduce by increasing the age of chicken,while vaccination group was decreased by increasing age,especially at 49 days and we need to consider another vaccination to get full protection. 展开更多
关键词 ANTIBODY VACCINATION CONTROL ND S/P ratio
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Growth Performance of Crocodile Fed with Chicken Intestine and Trash Fish at Smallholder Farm in Siem Reap Province,Cambodia
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作者 Sourloing Hor Kanan Dim +3 位作者 Sungchang Kang Kroesna Kang kouch theng Manay Ith 《Journal of Environmental Science and Engineering(B)》 CAS 2024年第2期86-91,共6页
The experiment was conducted at the smallholder crocodile farm at Pouk district,Siem reap province,and lasted 12 months,commenced from January 1st to December 31st,2023.The CRBD(Completely Randomized Block Design),was... The experiment was conducted at the smallholder crocodile farm at Pouk district,Siem reap province,and lasted 12 months,commenced from January 1st to December 31st,2023.The CRBD(Completely Randomized Block Design),was used in 3 treatments,such as T1(fed chicken intestine only),T2(fed chicken intestine+trash fish)and T3(fed trash fish only),with 4 replications in each treatment.The block was set by gender of crocodile,male and female.The 36 of local breed crocodiles 4 months old were allocated into each replication randomly.The result found that the length of crocodile gradually increased in the first semester,then it greatly increased from or for second semester for all treatments.In addition,the final body length of crocodile was significant different among treatments(p<0.001),the treatment of T2(fed chicken intestine+trash fish)had the longest one.Body length was also affected by gender,and male crocodile had longer body length(p<0.001).However,there were no significant differences for the interaction between types of feed with gender(p>0.05)(Table 1).The body weight of crocodile had gradually increased in the first semester,then it greatly increased from or for second semester for all treatments,while the final body weight of crocodile was also significant different among treatments(p<0.001)and the gender of crocodile(p<0.001).The average ADG(Daily Weight Gain)was significant different among treatments(p<0.001).Treatment of T2(fed chicken intestine+trash fish)had highest ADG,11.05 g,followed by T3(fed trash fish only),while the T1 had the lowest one.The gender of crocodile affected the ADG of crocodile,and male crocodile had higher ADG than female crocodile(p<0.001).However,there were no significant differences for the interaction between type of feed with gender(p>0.05).Feed intake was calculated based on amount of feed offer and measured in fresh basis.The amount of intake feed in all treatments was the same.FCR(Feed Conversion Ratio)was significant different among treatments(p<0.001)and also gender of crocodile(p<0.001).However,there were no significant differences for the interaction between types of feed with gender(p>0.05).In conclusion,the uses of trash fish and chicken intestine are an alternative feed source for crocodile to result in higher growth performance of crocodile. 展开更多
关键词 Local breed body length body weight ADG FCR
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Study on the Quality of Boar Semen in Kandal Province,Cambodia
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作者 Sophorn Kimsan Songchhang Kang +2 位作者 Kroesna Kang kouch theng Manay Ith 《Journal of Environmental Science and Engineering(B)》 CAS 2024年第2期92-95,共4页
In pig production,the selection of parent stock with high capacity and quality is a very important factor in order to obtain high-yield offspring.Therefore,a“study on the quality of boar semen in Kandal province”was... In pig production,the selection of parent stock with high capacity and quality is a very important factor in order to obtain high-yield offspring.Therefore,a“study on the quality of boar semen in Kandal province”was conducted for the purpose of comparing and evaluating the quality of boar semen(VAC:Volume Activity Concentration)between Duroc breeds,Yorkshire breeds and Landrace breeds.The study was carried out according to an observation study by selecting three breeds of boars around 3 years old,including two Duroc,two Yorkshire and two Landrace.Each boar was observed in three months,three times a month,and the interval was three to four days.The study took place in Takhmao City,Kandal Province,Cambodia.The results showed that the semen volume per ejaculate per head obtained from Yorkshire boar was an average of 250±76.16 mL,which was higher than the semen volume of Landrace with an average of 173.33±59.73 mL.And the semen volume of Duroc was an average of 149.44±72.70 mL.This value indicates that the semen volume per ejaculate per head was significantly different(p<0.01).The activity or mobility of spermatozoa in the semen between the three breeds differed with slightly significant difference(p<0.05),with the spermatozoa scored from the Yorkshire being 0.76±0.06,Landrace being 0.79±0.05 and Duroc being 0.83±0.03.The semen concentration showed that the Yorkshire had an average spermatozoa concentration of 568.89×10^(6) sperm cells/mL,and the Landrace had an average spermatozoa concentration of 321.11×10^(6) sperm cells/mL and the Duroc an average spermatozoa concentration of 568.86×10^(6) sperm cells/mL,which further confirmed that there was a significant difference in the statistical meaning(p<0.001).The semen quality(VAC)per one ejaculate between the three breeds showed significant difference(p<0.05),in which the VAC of the Yorkshire was 39.31×10^(9) sperms per ejaculate,Landrace was 44.54×10^(9) sperm cells per ejaculate and Duroc was 70.28×10^(9) sperm cells per ejaculate.Based on the above results,we can conclude that the semen quality(VAC)values of the three breeds of pigs are good,which can save the breeding because it shows the excellent quality of each breed. 展开更多
关键词 Pig breed semen volume spermatozoa mobility spermatozoa concentration
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Growth Efficiency of Local Chickens Using Sweet Potato Vine with Concentration Feed as a Basal Diet
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作者 Hieak Hun Suheang Sorn +4 位作者 Chan Bun Manay Ith Ty Chhay kouch theng Chetra Sar 《Journal of Environmental Science and Engineering(B)》 CAS 2024年第3期108-113,共6页
The experiment was conducted at the animal experimental station of the Faculty of Animal Sciences,Royal University of Agriculture,commenced from 10 January to 14 March,2022.The CRD(Completely Randomized Design)was use... The experiment was conducted at the animal experimental station of the Faculty of Animal Sciences,Royal University of Agriculture,commenced from 10 January to 14 March,2022.The CRD(Completely Randomized Design)was used with 5 treatments and 4 replications.The 500 chicks at one-day-old,local bread,were purchased from farmers who run their small scale hatchery farm.The chicks were kept together till 21 days old,and then were allocated randomly into in five diets(treatments),25 chicks per replication.The feed formulas for this study were designed and calculated to maintain a protein content of approximately 17%,dividing into 5 feed formulas(Treatments),such as concentrated feed only(control treatment:T0),plus 10%of fresh sweet potato vine(T1),plus 20%of fresh sweet potato vine(T2),plus 30%of fresh sweet potato vine(T3)and plus 20%of sweet potato vine meal(T4).The results showed that the treatment T1,had a similar final weight with the treatment T4,but was slightly lower than the treatment T0 which used concentrated feed alone.Feed intake and FCR(Feed Conversion Ratio)were not significantly different among those treatments.In conclusion,the average daily weight gain of chickens seemed to decrease with increasing the level of fresh sweet potato vine.Thus,the optimum level of fresh sweet potato vine was 10%,which may have little effect on the growth performance of local chickens.In addition,it was not far different from the positive control treatment and the treatment using sweet potato vine meal.However,the producers can increase the amount of sweet potato vine in feed formula by processing it into meal and mixing with concentrated feed or using as feed ingredients. 展开更多
关键词 Local breed final weight feed intake FCR
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Newcastle Disease in Chickens of Smallholder Farmers at Krang Yov Commune S’ang District,Kandal Province
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作者 Sokha Thim Chhay Kheang Lim +3 位作者 Kroesna Kang Sath Keo Vutey Venn kouch theng 《Journal of Environmental Science and Engineering(A)》 CAS 2024年第2期68-75,共8页
The study was conducted in Krang Yov commune,S’ang district,Kandal province and was conducted in three villages,including Kampong Po,Andong and Svay Damnak village.In the total,88 families were selected for interview... The study was conducted in Krang Yov commune,S’ang district,Kandal province and was conducted in three villages,including Kampong Po,Andong and Svay Damnak village.In the total,88 families were selected for interview by using snowball technic of nonprobability sampling.According to the results of the interviews,it showed that the number of farmers who raised chickens and had participated in training was very low number,ranging from 10.71%to 34.38%.For chicken raising system,the free ranging was the most popular by allowing the chicken to scavenge around the village,however,the total confinement was also applied.The local breed was more popular and the purpose of raising was for selling meat,however there were few households that raised the fighting cock for hobby.The number of chickens owned per household,was mostly less than 20 birds,while,the average number of birds per household ranged from 16.82 birds to 37.66 birds.However,the average number of birds per household was significant different between the households who have participated in chicken raising training and those who never participated(p<0.05),accounting for 47.52 birds and 19.85 birds,respectively.For the chicken feed,most of the farmers in those three villages used the concentration feed to offer the chicken,especially for supplementation,but,the whole rice was also used to supplement feed.The vaccination for chicken was significant different among the villages(p<0.05),in which the household in Kampong Po village had highest percentage of vaccinating their chicken,40.63%,than those in Svay Damnak and Andong village.The Newcastle vaccination was the more popular in those areas.But,all of the households have experienced with the disease,especially ND(Newcastle Disease)accounting for 67.05%,followed by influenza and fowl pox.When chicken got sick,most of farmers had treated their chicken with different methods,especially buying the medicine from the store and treatment by themselves were mostly applicable.Regarding the specific case of Newcastle disease,most of farmers were familiar with this disease and it frequently occurred in dry season and the knowledge on biosecurity measurement was very high,during suspected cases.However,the real practice with bird infected with Newcastle disease was limited since they treated the affected bird,and few farmers left their affected chicken in the flock,which could cause further spreading of this disease in the flock and village. 展开更多
关键词 Raising system VACCINATION BIOSECURITY ND
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Study on Preservation of Boar Semen and Bacteria Contamination in Kandal Province
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作者 Sophorn Kimsan Bunthon Chea +3 位作者 Manay Ith kouch theng Kroesna Kang Songchhang Kang 《Journal of Environmental Science and Engineering(A)》 CAS 2024年第6期231-235,共5页
The breeding program is progressing steadily,with increasing the awareness among farmers regarding the its critical role in improving productivity,promoting rapid growth,enhancing disease resistance,and boosting house... The breeding program is progressing steadily,with increasing the awareness among farmers regarding the its critical role in improving productivity,promoting rapid growth,enhancing disease resistance,and boosting household income.At the same time,the dissemination of AI(Artificila Insemination)technique among farmers has been active and effective.For the application of the AI,the quality of boar semen is extended in BTS(Belts Ville Thawing Solution)for more longer preservation period.The study purpose is to evaluate sperm survival time and bacterie contamination in boar semen.Six boars with different breed around 3 years old were used for this study as two Durocs,two Landraces and two Yorkshires.In evaluation sperm survival time in extender on each boar was designed by CRD(Completely Randomized Design)and calculating the survival time of spermatozoa was done after semen dilution and the sperm mobility was determined.The experimental BTS compositions are glucose,sodium bicarbonate acid,trisodium citrate,ethylenediaminetetraacetic acid,potassium chloride and distill water.In the same time,eighteen sample semen ware collected and used for detection of bacterial contamination.The results showed that the survival time of spermatozoa with its straight mobility more than 50%between the commercial BTS and BTS-KH was from 52 to 56 h.They are not significantly different(p>0.05).And the total survival time of spermatozoa was from 92 to 98 h,they are also not significantly different(p>0.05).The survival time of spermatozoa with its straight mobility more than 50%between the Yorkshire,Landrace and Duroc was 55 h 5 min,54 h 25 min and 50 h 18 min respectively.They are significantly different(p>0.05).The total survival time of spermatozoa between the commercial BTS and BTS-KH was 97 h 19 min,91 h 29 min and 92 h 26 min respectively.They are also not significantly different(p>0.05).The main bacteria isolated from the diluted semen were gram-negative bacteria,Escherichia coli 27.78%was contaminated with an average 6.4×106 CFU/mL,and the family Pseudomonaceae was 11.11%with 8.3×106 CFU/mL.In conclusion,the comparative results of the survival time of spematozoa during the breeding period(t=0.5)between the breeds and extender are similar.For bacteria contamination isolate from the semen all breeds may be due to direct and environmental factors.It is a rainy season with heavy rains that can cause infections. 展开更多
关键词 Pig breed BTS survival time bacteria contamination
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Effect of Using Israel Napier Grass in Feed Ration on Growth Performance of Local Chicken of Cambodia
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作者 Sophea Rous Tola Ka +2 位作者 Sokha Thim Sath Keo kouch theng 《Journal of Environmental Science and Engineering(B)》 2024年第1期47-52,共6页
This study investigated the effect of Israel grass in feed formulation on growth performance of local chicken.The study was conducted from October 2023 until January 2024 in Kampong Chheutel Institute of Technology.Th... This study investigated the effect of Israel grass in feed formulation on growth performance of local chicken.The study was conducted from October 2023 until January 2024 in Kampong Chheutel Institute of Technology.The CRD(Completely Randomized Design)was used with 4 treatments and 4 replications.The local chicks were purchased from local famer at 1 day old and kept in whole pen by feeding commercial feed.At 21 days old the total 320 chicks were allocated into experimental treatments.The 4 feed formulas based on the level of Israel grass added such as 0,5%,7%and 9%,were called T1(control),T2,T3 and T4 respectively.The result showed that body weight gain,feed intake,ADG(Average Daily Gain)and FCR(Feed Conversion Ratio)in all treatments were not significantly different(p>0.05).However,there was strong correlation between living weight with carcass of al treatments,but no difference among carcass percentage of local chicken for all different diet in this study.In conclusion,the Israel grass did not affect growth performance of local chickens and can be used to replace some expensive feed ingredient,such as rice bran. 展开更多
关键词 Israel grass growth performance CARCASS local chickens
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Cattle Production and Lumpy Skin Disease of Smallholder Farmers in Svay Rieng Province
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作者 Kimeang Chay Tola Ka +2 位作者 Sokun Khoeun Chann Bun kouch theng 《Journal of Environmental Science and Engineering(B)》 2024年第1期39-46,共8页
The cattle production in Romeas Haek district,Svay Rieng province,was developed remarkably,since there were some households raising cattle in large number,more than 20 heads.The grazing method of cattle was tethering ... The cattle production in Romeas Haek district,Svay Rieng province,was developed remarkably,since there were some households raising cattle in large number,more than 20 heads.The grazing method of cattle was tethering and free grassing in the commune grass,since the natural grass and rice straw were the main source of cattle feed,however some of them supplemented their cattle with crop-byproducts,planting grass or concentrated feed.For the majority,BCS(Body Condition Scoring)of the cattle in those areas was 3 accounting for 52%-73.33%,but it was found the thin cattle has low BCS of 2 accounting for 11.11%to 40.00%and also fat cattle has BCS of 4 up to 27.78%.However,the average BCS varied with village which ranged from 2.68 to 3.17,but is not different in term of commune and sex of cattle.Besides facing with shortage of feed,the experience with diseases was also another concern,since 85.71%to 95.83%of the households faced this problem last year.Among the clinical signs found,lumpy skin was 100%,i.e.no one escaped from this problem,thus making them aware of vaccinating their cattle against some diseases such as FMD(Foot and Mouth Disease),HS(Hemorrhagic Septicemia)and LSD(Lumpy Skin Disease),but mostly vaccination was used against FMD.Because the LSD has occurred in these areas since 2020,then the seroprevalence was found in high positive up to 72.62%by Enzyme-Linked Immunosorbent Assay(ELISA)test.This positive rate was also varying by villages,ranging from 53.3%to 94.44%.But there was no association between positive cattle with commune,BCS,sex and age of cattle.In conclusion,the cattle production in Romeas Haek district,Svay Rieng province had developed markedly,however the shortage of feed and disease occurrence are the main challenges for farmer cattle producers,especially LSD outbreak last year.However,most of the farmers have been aware of the benefit of vaccination and had vaccinated their cattle against those main disease,such as FMD,HS and LSD. 展开更多
关键词 Cattle production lumpy skin smallholder farmers seroprevalance
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Medication for Birth Control in Bitch at Battambang City,Battambang Province,Cambodia
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作者 Nal Si Sokun Khoeurn +1 位作者 Manay It kouch theng 《Journal of Environmental Science and Engineering(A)》 CAS 2024年第2期61-67,共7页
The observation study was conducted in Battambang City,Battambang province,by interviewing the 88 dog owners,who came to the animal pharmacy stores and clinics by using convenience sampling method of nonrandomized sam... The observation study was conducted in Battambang City,Battambang province,by interviewing the 88 dog owners,who came to the animal pharmacy stores and clinics by using convenience sampling method of nonrandomized sampling.Though the results of the interviews,showed that the dog owners were selected in different range of age and gender,however,most of them were in middle age from 21-40 years old,with medium and rich living wellbeing.The confinement in premise/house was primarily applied by dog owners.The number of bitches per household was from 1 to 3 batches,and there was no association with the wellbeing of the owners,and the age was from 2 to 4 years old,but some bitches had older age.Most of the bitches were dewormed in last 3 months and 6 months,however,there were some bitches last more than 6 months after deworming.The bitch vaccination was applied by owner around for 60.00%.There were two popular types of vaccination,Rabies and DHLPP(Distemper,Hepatitis,Leptospirosis,Parvovirus,and Parainfluenza).For dog population management,about 94.29%of the owners apply nonsurgical method with applying medicine.The reasons for using nonsurgical method were not only the cheapest price and easy way,but also there was no information on the consequence of using medication for birth control.The medication was highly used before heat.But,almost half of them got health problem in less than 3 months after administration,also some got long-term effect.Among clinical signs observed,the enlargement of belly was the most evidence,since 54.76%of affected bitches had shown it,then followed by discharge blood from vulvar,clear discharge and thick white pus from vulvar,accounting for 38.10%,35.71%and 26.71%,respectively. 展开更多
关键词 BITCH VACCINATION birth control clinical signs
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