Open clusters are the basic building blocks that serve as a laboratory for the study of young stellar populations in the Milky Way.Variable stars in open clusters provide a unique way to accurately probe the internal ...Open clusters are the basic building blocks that serve as a laboratory for the study of young stellar populations in the Milky Way.Variable stars in open clusters provide a unique way to accurately probe the internal structure,temporal and dynamical evolutionary stages of individual stars and the host cluster.The most powerful tool for such studies is time-domain photometric observations.This paper follows the route of our previous work,concentrating on a photometric search for variable stars in NGC 884.The target cluster is the companion of NGC869,forming the well-known double cluster system that is gravitationally bound.From the observation run in 2016 November,a total of 9247 B-band CCD images and 8218Ⅴ-band CCD images were obtained.We detected a total of 15 stars with variability in visual brightness,including five Be stars,three eclipsing binaries,and seven of unknown types.Two new variable stars were discovered in this work.We also compared the variable star content of NGC 884 with its companion NGC 869.展开更多
The high energy coordination compounds Cu(TZCA)_(2)(ClO_(4))_(2)(ECCs-1) was prepared by 1H-tetrazole-5-carbohydrazide(TZCA) with a high energy skeleton and a strong coordination ability group.At the same time,the rea...The high energy coordination compounds Cu(TZCA)_(2)(ClO_(4))_(2)(ECCs-1) was prepared by 1H-tetrazole-5-carbohydrazide(TZCA) with a high energy skeleton and a strong coordination ability group.At the same time,the reaction activity of the ligand was explored,and the single crystal structure of it and intermediate were obtained.The structures of all substances were characterized by IR and EA.And the structure and composition of ECCs-1 are confirmed by ESP,AC,SEM and ICP-OES.Physical and chemical properties tests show that ECCs-1 has an acceptable thermal stability(T_(d)=177℃) and extremely sensitive mechanical stimulation(IS=1 J,FS=5 N).The comprehensive performance test results show that ECCs-1 has excellent initiation ability.In addition,the decomposition mechanism of ECCs-1 is explored from two aspects of experiment and theoretical calculation.展开更多
Revealing the localized corrosion process of Mg alloy is considered as one of the most significant ways for improving its corrosion resistance.The reliable monitor should be high distinguishability and real-time in li...Revealing the localized corrosion process of Mg alloy is considered as one of the most significant ways for improving its corrosion resistance.The reliable monitor should be high distinguishability and real-time in liquid environment.Herein,Mg-9Al-1Fe and Mg-9Al-1Fe-1Gd alloys were designed to highlight the impact of intermetallic on the corrosion behaviour.In-situ AFM with a special electrolyte circulation system and quasi-in-situ SEM observation were used to monitor the corrosion process of the designed alloys.SEM-EDS and TEM-SAED were applied to identify the intermetallic in the designed alloys,and their volta potentials were measured by SKPFM.According to the real-time and real-space in-situ AFM monitor,the corrosion process consisted of dissolution of anodicα-Mg phase,accumulation of corrosion products around cathodic phase and shedding of some fine cathodic phase.Then,the localized corrosion process of Mg alloy was revealed combined with the results of the monitor of corrosion process and Volta potential difference.展开更多
Electrochemical impedance spectroscopy(EIS)flow cytometry offers the advantages of speed,affordability,and portability in cell analysis and cytometry applications.However,the integration challenges of microfluidic and...Electrochemical impedance spectroscopy(EIS)flow cytometry offers the advantages of speed,affordability,and portability in cell analysis and cytometry applications.However,the integration challenges of microfluidic and EIS read-out circuits hinder the downsizing of cytometry devices.To address this,we developed a thermal-bubble-driven impedance flow cytometric application-specific integrated circuit(ASIC).The thermal-bubble micropump avoids external piping and equipment,enabling high-throughput designs.With a total of 36 cell counting channels,each measuring 884×220μm2,the chip significantly enhances the throughput of flow cytometers.Each cell counting channel incorporates a differential trans-impedance amplifier(TIA)to amplify weak biosensing signals.By eliminating the parasitic parameters created at the complementary metal-oxidesemiconductor transistor(CMOS)-micro-electromechanical systems(MEMS)interface,the counting accuracy can be increased.The on-chip TIA can adjust feedback resistance from 5 to 60 kΩto accommodate solutions with different impedances.The chip effectively classifies particles of varying sizes,demonstrated by the average peak voltages of 0.0529 and 0.4510 mV for 7 and 14μm polystyrene beads,respectively.Moreover,the counting accuracies of the chip for polystyrene beads and MSTO-211H cells are both greater than 97.6%.The chip exhibits potential for impedance flow cytometer at low cost,high-throughput,and miniaturization for the application of point-of-care diagnostics.展开更多
Identification of the S genotype of Malus plants will greatly promote the discovery of new genes,the cultivation and production of apple,the breeding of new varieties,and the origin and evolution of self-incompatibili...Identification of the S genotype of Malus plants will greatly promote the discovery of new genes,the cultivation and production of apple,the breeding of new varieties,and the origin and evolution of self-incompatibility in Malus plants.In this experiment,88 Malus germplasm resources,such as Aihuahong,Xishuhaitang,and Reguanzi,were used as materials.Seven gene-specific primer combinations were used in the genotype identification.PCR amplification using leaf DNA produced a single S-RNase gene fragment in all materials.The results revealed that 70 of the identified materials obtained a complete S-RNase genotype,while only one S-RNase gene was found in 18 of them.Through homology comparison and analysis,13 S-RNase genotypes were obtained:S_(1)S_(2)(Aihuahong,etc.),S_(1)S_(28)(Xixian Haitang,etc.),S_(1)S_(51)(Hebei Pingdinghaitang),S_(1)S_(3)(Xiangyangcun Daguo,etc.),S_(2)S_(3)(Zhaiyehaitang,etc.),S_(3)S_(51)(Xishan 1),S_(3)S_(28)(Huangselihaerde,etc.),S_(2)S_(28)(Honghaitang,etc.),S_(4)S_(28)(Bo 11),S_(7)S_(28)(Jiuquan Shaguo),S_(10)S_e(Dongchengguan 13),S_(10)S_(21)(Dongxiangjiao)and S_(3)S_(51)(Xiongyue Haitang).Simultaneously,the frequency of the S gene in the tested materials was analyzed.The findings revealed that different S genes had varying frequencies in Malus resources,as well as varying frequencies between intraspecific and interspecific.S_(3) had the highest frequency of 68.18%,followed by S_(1)(42.04%).In addition,the phylogenetic tree and origin evolution analysis revealed that the S gene differentiation was completed prior to the formation of various apple species,that cultivated species also evolved new S genes,and that the S_(50) gene is the oldest S allele in Malus plants.The S_(1),S_(29),and S_(33) genes in apple-cultivated species,on the other hand,may have originated in M.sieversii,M.hupehensis,and M.kansuensis,respectively.In addition to M.sieversii,M.kansuensis and M.sikkimensis may have also played a role in the origin and evolution of some Chinese apples.展开更多
Food allergy as a global health problem threatens food industry.Bee pollen(BP)is a typical food with allergenic potentials,although it performs various nutritional/pharmacological functions to humans.In this study,lac...Food allergy as a global health problem threatens food industry.Bee pollen(BP)is a typical food with allergenic potentials,although it performs various nutritional/pharmacological functions to humans.In this study,lactic acid bacteria(LAB)were used to ferment Brassica napus BP for alleviating its allergenicity.Four novel allergens(glutaredoxin,oleosin-B2,catalase and lipase)were identified with significant decreases in LAB-fermented BP(FBP)than natural BP by proteomics.Meanwhile,metabolomics analysis showed significant increases of 28 characteristic oligopeptides and amino acids in FBP versus BP,indicating the degradation of LAB on allergens.Moreover,FBP showed alleviatory effects in BALB/c mice,which relieved pathological symptoms and lowered production of allergic mediators.Microbial high-throughput sequencing analysis showed that FBP could regulate gut microbiota and metabolism to strengthen immunity,which were closely correlated with the alleviation of allergic reactivity.These findings could contribute to the development and utilization of hypoallergenic BP products.展开更多
Deep shale gas reserves that have been fractured typically have many relatively close perforation holes. Due to theproximity of each fracture during the formation of the fracture network, there is significant stress i...Deep shale gas reserves that have been fractured typically have many relatively close perforation holes. Due to theproximity of each fracture during the formation of the fracture network, there is significant stress interference,which results in uneven fracture propagation. It is common practice to use “balls” to temporarily plug fractureopenings in order to lessen liquid intake and achieve uniform propagation in each cluster. In this study, a diameteroptimization model is introduced for these plugging balls based on a multi-cluster fracture propagationmodel and a perforation dynamic abrasion model. This approach relies on proper consideration of the multiphasenature of the considered problem and the interaction force between the involved fluid and solid phases. Accordingly,it can take into account the behavior of the gradually changing hole diameter due to proppant continuousperforation erosion. Moreover, it can provide useful information about the fluid-dynamic behavior of the consideredsystem before and after plugging. It is shown that when the diameter of the temporary plugging ball is1.2 times that of the perforation hole, the perforation holes of each cluster can be effectively blocked.展开更多
Background:The influenza A virus is the primary cause of respiratory infections and poses a global health risk.Pudilan Xiaoyan oral liquid(PDL)exhibits anti-inflammatory and immunomodulatory properties.PDL is commonly...Background:The influenza A virus is the primary cause of respiratory infections and poses a global health risk.Pudilan Xiaoyan oral liquid(PDL)exhibits anti-inflammatory and immunomodulatory properties.PDL is commonly employed in clinical practice to manage upper respiratory tract infections.However,there is still much to uncover regarding its potential therapeutic mechanism.Methods:Institute of cancer research mice were infected with influenza A virus via nasal drip.The general state of the mice,lung index,and lung index inhibition rate were used to evaluate the efficacy of PDL.Enzyme-linked immunosorbent assay,western blotting,and immunohistochemistry were used to observe the presence of proteins and cytokines in the lung tissue.Apoptosis was evaluated using the TUNEL assay.Results:PDL improved the mental state of influenza A virus-infected mice,reduced the lung index,and inhibited viral replication.The expression of interleukin-1βand tumor necrosis factor-αwere decreased,whereas the expression of interleukin-10 in the lung tissue was increased due to PDL treatment.In addition,PDL treatment modulated Toll-like receptor 4 and MyD88 expressions in the lung tissues.PDL significantly reduced apoptosis and decreased cleaved caspase-3 and PARP levels,whereas increased B-cell lymphoma-2 expression in the lung tissue.Notably,the moderate-dose group of PDL exhibited a more pronounced effect.These findings indicate that PDL exerts a protective effect against pneumonia injury in influenza A virus-infected mice.Conclusion:PDL inhibited the inflammatory response and regulated apoptosis by regulating Toll-like receptor 4 and MyD88 protein expressions,thereby protecting the lung tissue from viral infection-induced lung tissue injury.展开更多
Copper,as an essential trace nutrient,plays a crucial role in biological processes such as mitochondrial respiration,antioxidant stress response,and the synthesis of biomolecules.Typically,cellular copper concentratio...Copper,as an essential trace nutrient,plays a crucial role in biological processes such as mitochondrial respiration,antioxidant stress response,and the synthesis of biomolecules.Typically,cellular copper concentrations are maintained at very low levels,a pattern also observed in cancer cells to prevent adverse consequences of copper overload,such as cuproptosis.This involves copper dependency,accumulation of lipidated proteins,and a reduction in Fe-S cluster proteins[1].Various neurodegenerative diseases are associated with imbalances in copper homeostasis.展开更多
In view of the problems of inconsistent data semantics,inconsistent data formats,and difficult data quality assurance between the railway engineering design phase and the construction and operation phase,as well as th...In view of the problems of inconsistent data semantics,inconsistent data formats,and difficult data quality assurance between the railway engineering design phase and the construction and operation phase,as well as the difficulty in fully realizing the value of design results,this paper proposes a design and implementation scheme for a railway engineering collaborative design platform.The railway engineering collaborative design platform mainly includes functional modules such as metadata management,design collaboration,design delivery management,model component library,model rendering services,and Building Information Modeling(BIM)application services.Based on this,research is conducted on multi-disciplinary parameterized collaborative design technology for railway engineering,infrastructure data management and delivery technology,and design multi-source data fusion and application technology.The railway engineering collaborative design platform is compared with other railway design software to further validate its advantages and advanced features.The platform has been widely applied in multiple railway construction projects,greatly improving the design and project management efficiency.展开更多
At present, the Omicron variant is still the dominant strain in the global novel coronavirus pneumonia pandemic, and has the characteristics of concealed transmission, which brings heavy pressure to the health systems...At present, the Omicron variant is still the dominant strain in the global novel coronavirus pneumonia pandemic, and has the characteristics of concealed transmission, which brings heavy pressure to the health systems of different countries. Omicron infections were first found in Chinese Mainland in Tianjin in December 2021, and Omicron epidemic broke out in many parts of China in 2022. In order to enable the country and government to make scientific and accurate decisions in the face of the epidemic, it is particularly important to predict and analyze the relevant factors of Omicron’s covert transmission. In this paper, based on the official data of Jilin City and the improved SEIR dynamic model, through parameter estimation, the contact infection probability of symptomatic infected persons in Omicron infected patients is 0.4265, and the attenuation factor is 0.1440. Secondly, the influence of infectious duration in different incubation periods, asymptomatic infected persons and other factors on the epidemic situation in this area was compared. Finally, the scale of epidemic development was predicted and analyzed.展开更多
Li-metal batteries(LMBs)regain research prominence owing to the ever-increasing high-energy requirements.Commercially available carbonate electrolytes exhibit unfavourable parasitic reactions with Limetal anode(LMA),l...Li-metal batteries(LMBs)regain research prominence owing to the ever-increasing high-energy requirements.Commercially available carbonate electrolytes exhibit unfavourable parasitic reactions with Limetal anode(LMA),leading to the formation of unstable solid electrolyte interphase(SEI)and the breed of Li dendrites/dead Li.Significantly,lithium nitrate(LiNO_(3)),an excellent film-forming additive,proves crucial to construct a robust Li_(3)N/Li_(2)O/Li_(x)NO_(y)-rich SEI after combining with ether-based electrolytes.Thus,the given challenge leads to natural ideas which suggest the incorporation of LiNO_(3) into commercial carbonate for practical LMBs.Regrettably,LiNO_(3) demonstrates limited solubility(~800 ppm)in commercial carbonate electrolytes.Thence,developing stable SEI and dendrite-free LMA with the incorporation of LiNO_(3) into carbonate electrolytes is an efficacious strategy to realize robust LMBs via a scalable and cost-effective route.Therefore,this review unravels the grievances between LMA,LiNO_(3)and carbonate electrolytes,and enables a comprehensive analysis of LMA stabilizing mechanism with LiNO_(3),dissolution principle of LiNO_(3) in carbonate electrolytes,and LiNO_(3) introduction strategies.This review converges attention on a point that the LiNO_(3)-introduction into commercial carbonate electrolytes is an imperious choice to realize practical LMBs with commercial 4 V layered cathode.展开更多
Countries are duly focusing more on biomass resources because of the increasing oil crisis.Owing to their excellent properties,such as natural characteristics,good mechanical performance,and outstanding chemical prope...Countries are duly focusing more on biomass resources because of the increasing oil crisis.Owing to their excellent properties,such as natural characteristics,good mechanical performance,and outstanding chemical properties,cellulose-based materials are highly valued as promising bioderived nanomaterials,especially bacterial cellulose(BC).The main advantage lies in eliminating the problem of removing lignin and hemicellulose from woody cellulose.Moreover,the use of BC reduces the consumption of wood,the excessive use of which aggravates global warming.Herein,we summarize the applications of BC composites in filter,medical,and conductive materials,and other fields.This review contributes to further expand the applications of this renewable polymer.展开更多
In vivo imaging of cerebral ischemia/reperfusion injury remains an important challenge.We injected porous Ag/Au@SiO_(2) bimetallic hollow nanoshells carrying anti-tropomyosin 4 as a molecular probe into mice with cere...In vivo imaging of cerebral ischemia/reperfusion injury remains an important challenge.We injected porous Ag/Au@SiO_(2) bimetallic hollow nanoshells carrying anti-tropomyosin 4 as a molecular probe into mice with cerebral ischemia/reperfusion injury and observed microvascular changes in the brain using photoacoustic imaging with ultrasonography.At each measured time point,the total photoacoustic signal was significantly higher on the affected side than on the healthy side.Twelve hours after reperfusion,cerebral perfusion on the affected side increased,cerebrovascular injury worsened,and anti-tropomyosin 4 expression increased.Twenty-four hours after reperfusion and later,perfusion on the affected side declined slowly and stabilized after 1 week;brain injury was also alleviated.Histopathological and immunohistochemical examinations confirmed the brain injury tissue changes.The nanoshell molecular probe carrying anti-tropomyosin 4 has potential for use in early diagnosis of cerebral ischemia/reperfusion injury and evaluating its progression.展开更多
At the beginning of the Cenozoic,the atmospheric CO_(2)concentration increased rapidly from~2000 ppmv at 60 Ma to~4600 ppmv at 51 Ma,which is 5–10 times higher than the present value,and then continuous declined from...At the beginning of the Cenozoic,the atmospheric CO_(2)concentration increased rapidly from~2000 ppmv at 60 Ma to~4600 ppmv at 51 Ma,which is 5–10 times higher than the present value,and then continuous declined from~51 to 34 Ma.The cause of this phenomenon is still not well understood.In this study,we demonstrate that the initiation of Cenozoic west Pacific plate subduction,triggered by the hard collision in the Tibetan Plateau,occurred at approximately 51 Ma,coinciding with the tipping point.The water depths of the Pacific subduction zones are mostly below the carbonate compensation depths,while those of the Neo-Tethys were much shallower before the collision and caused far more carbonate subducting.Additionally,more volcanic ashes erupted from the west Pacific subduction zones,which consume CO_(2).The average annual west Pacific volvano eruption is 1.11 km~3,which is higher than previous estimations.The amount of annual CO_(2)absorbed by chemical weathering of additional west Pacific volcanic ashes could be comparable to the silicate weathering by the global river.We propose that the initiation of the western Pacific subduction controlled the long-term reduction of atmospheric CO_(2)concentration.展开更多
基金the National Natural Science Foundation of China(NSFC)through grants 12003022,12373035,12233009 and 12173047support from the Youth Innovation Promotion Association of the CAS(grant No.2022055)。
文摘Open clusters are the basic building blocks that serve as a laboratory for the study of young stellar populations in the Milky Way.Variable stars in open clusters provide a unique way to accurately probe the internal structure,temporal and dynamical evolutionary stages of individual stars and the host cluster.The most powerful tool for such studies is time-domain photometric observations.This paper follows the route of our previous work,concentrating on a photometric search for variable stars in NGC 884.The target cluster is the companion of NGC869,forming the well-known double cluster system that is gravitationally bound.From the observation run in 2016 November,a total of 9247 B-band CCD images and 8218Ⅴ-band CCD images were obtained.We detected a total of 15 stars with variability in visual brightness,including five Be stars,three eclipsing binaries,and seven of unknown types.Two new variable stars were discovered in this work.We also compared the variable star content of NGC 884 with its companion NGC 869.
基金projects of National Natural Science Foundation of China (Grant Nos.22175025 and 21905023) for their generous financial support。
文摘The high energy coordination compounds Cu(TZCA)_(2)(ClO_(4))_(2)(ECCs-1) was prepared by 1H-tetrazole-5-carbohydrazide(TZCA) with a high energy skeleton and a strong coordination ability group.At the same time,the reaction activity of the ligand was explored,and the single crystal structure of it and intermediate were obtained.The structures of all substances were characterized by IR and EA.And the structure and composition of ECCs-1 are confirmed by ESP,AC,SEM and ICP-OES.Physical and chemical properties tests show that ECCs-1 has an acceptable thermal stability(T_(d)=177℃) and extremely sensitive mechanical stimulation(IS=1 J,FS=5 N).The comprehensive performance test results show that ECCs-1 has excellent initiation ability.In addition,the decomposition mechanism of ECCs-1 is explored from two aspects of experiment and theoretical calculation.
基金support by the National Natural Science Foundation of China(51961026)the Interdisciplinary Innovation Fund of Nanchang University(Project No.2019-9166-27060003).
文摘Revealing the localized corrosion process of Mg alloy is considered as one of the most significant ways for improving its corrosion resistance.The reliable monitor should be high distinguishability and real-time in liquid environment.Herein,Mg-9Al-1Fe and Mg-9Al-1Fe-1Gd alloys were designed to highlight the impact of intermetallic on the corrosion behaviour.In-situ AFM with a special electrolyte circulation system and quasi-in-situ SEM observation were used to monitor the corrosion process of the designed alloys.SEM-EDS and TEM-SAED were applied to identify the intermetallic in the designed alloys,and their volta potentials were measured by SKPFM.According to the real-time and real-space in-situ AFM monitor,the corrosion process consisted of dissolution of anodicα-Mg phase,accumulation of corrosion products around cathodic phase and shedding of some fine cathodic phase.Then,the localized corrosion process of Mg alloy was revealed combined with the results of the monitor of corrosion process and Volta potential difference.
基金supported by the Key Project of the National Natural Science Foundation of China(Grant No.82130069).
文摘Electrochemical impedance spectroscopy(EIS)flow cytometry offers the advantages of speed,affordability,and portability in cell analysis and cytometry applications.However,the integration challenges of microfluidic and EIS read-out circuits hinder the downsizing of cytometry devices.To address this,we developed a thermal-bubble-driven impedance flow cytometric application-specific integrated circuit(ASIC).The thermal-bubble micropump avoids external piping and equipment,enabling high-throughput designs.With a total of 36 cell counting channels,each measuring 884×220μm2,the chip significantly enhances the throughput of flow cytometers.Each cell counting channel incorporates a differential trans-impedance amplifier(TIA)to amplify weak biosensing signals.By eliminating the parasitic parameters created at the complementary metal-oxidesemiconductor transistor(CMOS)-micro-electromechanical systems(MEMS)interface,the counting accuracy can be increased.The on-chip TIA can adjust feedback resistance from 5 to 60 kΩto accommodate solutions with different impedances.The chip effectively classifies particles of varying sizes,demonstrated by the average peak voltages of 0.0529 and 0.4510 mV for 7 and 14μm polystyrene beads,respectively.Moreover,the counting accuracies of the chip for polystyrene beads and MSTO-211H cells are both greater than 97.6%.The chip exhibits potential for impedance flow cytometer at low cost,high-throughput,and miniaturization for the application of point-of-care diagnostics.
基金financially supported by the Agricultural Science and Technology Innovation Program(CAASASTIP-2021-RIP-02)。
文摘Identification of the S genotype of Malus plants will greatly promote the discovery of new genes,the cultivation and production of apple,the breeding of new varieties,and the origin and evolution of self-incompatibility in Malus plants.In this experiment,88 Malus germplasm resources,such as Aihuahong,Xishuhaitang,and Reguanzi,were used as materials.Seven gene-specific primer combinations were used in the genotype identification.PCR amplification using leaf DNA produced a single S-RNase gene fragment in all materials.The results revealed that 70 of the identified materials obtained a complete S-RNase genotype,while only one S-RNase gene was found in 18 of them.Through homology comparison and analysis,13 S-RNase genotypes were obtained:S_(1)S_(2)(Aihuahong,etc.),S_(1)S_(28)(Xixian Haitang,etc.),S_(1)S_(51)(Hebei Pingdinghaitang),S_(1)S_(3)(Xiangyangcun Daguo,etc.),S_(2)S_(3)(Zhaiyehaitang,etc.),S_(3)S_(51)(Xishan 1),S_(3)S_(28)(Huangselihaerde,etc.),S_(2)S_(28)(Honghaitang,etc.),S_(4)S_(28)(Bo 11),S_(7)S_(28)(Jiuquan Shaguo),S_(10)S_e(Dongchengguan 13),S_(10)S_(21)(Dongxiangjiao)and S_(3)S_(51)(Xiongyue Haitang).Simultaneously,the frequency of the S gene in the tested materials was analyzed.The findings revealed that different S genes had varying frequencies in Malus resources,as well as varying frequencies between intraspecific and interspecific.S_(3) had the highest frequency of 68.18%,followed by S_(1)(42.04%).In addition,the phylogenetic tree and origin evolution analysis revealed that the S gene differentiation was completed prior to the formation of various apple species,that cultivated species also evolved new S genes,and that the S_(50) gene is the oldest S allele in Malus plants.The S_(1),S_(29),and S_(33) genes in apple-cultivated species,on the other hand,may have originated in M.sieversii,M.hupehensis,and M.kansuensis,respectively.In addition to M.sieversii,M.kansuensis and M.sikkimensis may have also played a role in the origin and evolution of some Chinese apples.
基金supported by the National Natural Science Foundation of China(32102605)the Agricultural Science and Technology Innovation Program under Grant(CAAS-ASTIP-2020-IAR)。
文摘Food allergy as a global health problem threatens food industry.Bee pollen(BP)is a typical food with allergenic potentials,although it performs various nutritional/pharmacological functions to humans.In this study,lactic acid bacteria(LAB)were used to ferment Brassica napus BP for alleviating its allergenicity.Four novel allergens(glutaredoxin,oleosin-B2,catalase and lipase)were identified with significant decreases in LAB-fermented BP(FBP)than natural BP by proteomics.Meanwhile,metabolomics analysis showed significant increases of 28 characteristic oligopeptides and amino acids in FBP versus BP,indicating the degradation of LAB on allergens.Moreover,FBP showed alleviatory effects in BALB/c mice,which relieved pathological symptoms and lowered production of allergic mediators.Microbial high-throughput sequencing analysis showed that FBP could regulate gut microbiota and metabolism to strengthen immunity,which were closely correlated with the alleviation of allergic reactivity.These findings could contribute to the development and utilization of hypoallergenic BP products.
基金supported by the National Natural Science Foundation of China (No.U21B2071).
文摘Deep shale gas reserves that have been fractured typically have many relatively close perforation holes. Due to theproximity of each fracture during the formation of the fracture network, there is significant stress interference,which results in uneven fracture propagation. It is common practice to use “balls” to temporarily plug fractureopenings in order to lessen liquid intake and achieve uniform propagation in each cluster. In this study, a diameteroptimization model is introduced for these plugging balls based on a multi-cluster fracture propagationmodel and a perforation dynamic abrasion model. This approach relies on proper consideration of the multiphasenature of the considered problem and the interaction force between the involved fluid and solid phases. Accordingly,it can take into account the behavior of the gradually changing hole diameter due to proppant continuousperforation erosion. Moreover, it can provide useful information about the fluid-dynamic behavior of the consideredsystem before and after plugging. It is shown that when the diameter of the temporary plugging ball is1.2 times that of the perforation hole, the perforation holes of each cluster can be effectively blocked.
基金funded by Scientific and Technological Innovation Project of China Academy of Chinese Medical Sciences,grant number CI2021A04608National Natural Science Foundation of China,grant number 82141206.
文摘Background:The influenza A virus is the primary cause of respiratory infections and poses a global health risk.Pudilan Xiaoyan oral liquid(PDL)exhibits anti-inflammatory and immunomodulatory properties.PDL is commonly employed in clinical practice to manage upper respiratory tract infections.However,there is still much to uncover regarding its potential therapeutic mechanism.Methods:Institute of cancer research mice were infected with influenza A virus via nasal drip.The general state of the mice,lung index,and lung index inhibition rate were used to evaluate the efficacy of PDL.Enzyme-linked immunosorbent assay,western blotting,and immunohistochemistry were used to observe the presence of proteins and cytokines in the lung tissue.Apoptosis was evaluated using the TUNEL assay.Results:PDL improved the mental state of influenza A virus-infected mice,reduced the lung index,and inhibited viral replication.The expression of interleukin-1βand tumor necrosis factor-αwere decreased,whereas the expression of interleukin-10 in the lung tissue was increased due to PDL treatment.In addition,PDL treatment modulated Toll-like receptor 4 and MyD88 expressions in the lung tissues.PDL significantly reduced apoptosis and decreased cleaved caspase-3 and PARP levels,whereas increased B-cell lymphoma-2 expression in the lung tissue.Notably,the moderate-dose group of PDL exhibited a more pronounced effect.These findings indicate that PDL exerts a protective effect against pneumonia injury in influenza A virus-infected mice.Conclusion:PDL inhibited the inflammatory response and regulated apoptosis by regulating Toll-like receptor 4 and MyD88 protein expressions,thereby protecting the lung tissue from viral infection-induced lung tissue injury.
文摘Copper,as an essential trace nutrient,plays a crucial role in biological processes such as mitochondrial respiration,antioxidant stress response,and the synthesis of biomolecules.Typically,cellular copper concentrations are maintained at very low levels,a pattern also observed in cancer cells to prevent adverse consequences of copper overload,such as cuproptosis.This involves copper dependency,accumulation of lipidated proteins,and a reduction in Fe-S cluster proteins[1].Various neurodegenerative diseases are associated with imbalances in copper homeostasis.
基金supported by the National Key Research and Development Program of China(2021YFB2600405).
文摘In view of the problems of inconsistent data semantics,inconsistent data formats,and difficult data quality assurance between the railway engineering design phase and the construction and operation phase,as well as the difficulty in fully realizing the value of design results,this paper proposes a design and implementation scheme for a railway engineering collaborative design platform.The railway engineering collaborative design platform mainly includes functional modules such as metadata management,design collaboration,design delivery management,model component library,model rendering services,and Building Information Modeling(BIM)application services.Based on this,research is conducted on multi-disciplinary parameterized collaborative design technology for railway engineering,infrastructure data management and delivery technology,and design multi-source data fusion and application technology.The railway engineering collaborative design platform is compared with other railway design software to further validate its advantages and advanced features.The platform has been widely applied in multiple railway construction projects,greatly improving the design and project management efficiency.
文摘At present, the Omicron variant is still the dominant strain in the global novel coronavirus pneumonia pandemic, and has the characteristics of concealed transmission, which brings heavy pressure to the health systems of different countries. Omicron infections were first found in Chinese Mainland in Tianjin in December 2021, and Omicron epidemic broke out in many parts of China in 2022. In order to enable the country and government to make scientific and accurate decisions in the face of the epidemic, it is particularly important to predict and analyze the relevant factors of Omicron’s covert transmission. In this paper, based on the official data of Jilin City and the improved SEIR dynamic model, through parameter estimation, the contact infection probability of symptomatic infected persons in Omicron infected patients is 0.4265, and the attenuation factor is 0.1440. Secondly, the influence of infectious duration in different incubation periods, asymptomatic infected persons and other factors on the epidemic situation in this area was compared. Finally, the scale of epidemic development was predicted and analyzed.
基金supported by the National Natural Science Foundation of China(Nos.U1710257,U1702253,U1903129)the National Key R&D Program of China(Nos.2017YFC0210403,2017YFC0210404)。
基金the support by the Leading Innovative and Entrepreneur Team Introduction Program of Zhejiang(2019R01006)the National Natural Science Foundation of China(NSFC:12205252)+3 种基金A Project Supported by Scientific Research Fund of Zhejiang Provincial Education Department(Y202250795)the Research Funds of Institute of Zhejiang University-Quzhou,the Basic Public Welfare Research Special Project of Zhejiang Province(LZY22B040001)the Science and Technology Project of Quzhou Research Institute,Zhejiang University(IZQ2021KJ2032)the Independent Scientific Research Project of Quzhou Research Institute,Zhejiang University(IZQ2021RCZX007)。
文摘Li-metal batteries(LMBs)regain research prominence owing to the ever-increasing high-energy requirements.Commercially available carbonate electrolytes exhibit unfavourable parasitic reactions with Limetal anode(LMA),leading to the formation of unstable solid electrolyte interphase(SEI)and the breed of Li dendrites/dead Li.Significantly,lithium nitrate(LiNO_(3)),an excellent film-forming additive,proves crucial to construct a robust Li_(3)N/Li_(2)O/Li_(x)NO_(y)-rich SEI after combining with ether-based electrolytes.Thus,the given challenge leads to natural ideas which suggest the incorporation of LiNO_(3) into commercial carbonate for practical LMBs.Regrettably,LiNO_(3) demonstrates limited solubility(~800 ppm)in commercial carbonate electrolytes.Thence,developing stable SEI and dendrite-free LMA with the incorporation of LiNO_(3) into carbonate electrolytes is an efficacious strategy to realize robust LMBs via a scalable and cost-effective route.Therefore,this review unravels the grievances between LMA,LiNO_(3)and carbonate electrolytes,and enables a comprehensive analysis of LMA stabilizing mechanism with LiNO_(3),dissolution principle of LiNO_(3) in carbonate electrolytes,and LiNO_(3) introduction strategies.This review converges attention on a point that the LiNO_(3)-introduction into commercial carbonate electrolytes is an imperious choice to realize practical LMBs with commercial 4 V layered cathode.
基金supported by grants from the National Key Research and Development Program of China(2022YFD2200804)National Natural Science Foundation of China(No.31770622).
文摘Countries are duly focusing more on biomass resources because of the increasing oil crisis.Owing to their excellent properties,such as natural characteristics,good mechanical performance,and outstanding chemical properties,cellulose-based materials are highly valued as promising bioderived nanomaterials,especially bacterial cellulose(BC).The main advantage lies in eliminating the problem of removing lignin and hemicellulose from woody cellulose.Moreover,the use of BC reduces the consumption of wood,the excessive use of which aggravates global warming.Herein,we summarize the applications of BC composites in filter,medical,and conductive materials,and other fields.This review contributes to further expand the applications of this renewable polymer.
基金supported by the National Natural Science Foundation of China,No.81730050(to WH).
文摘In vivo imaging of cerebral ischemia/reperfusion injury remains an important challenge.We injected porous Ag/Au@SiO_(2) bimetallic hollow nanoshells carrying anti-tropomyosin 4 as a molecular probe into mice with cerebral ischemia/reperfusion injury and observed microvascular changes in the brain using photoacoustic imaging with ultrasonography.At each measured time point,the total photoacoustic signal was significantly higher on the affected side than on the healthy side.Twelve hours after reperfusion,cerebral perfusion on the affected side increased,cerebrovascular injury worsened,and anti-tropomyosin 4 expression increased.Twenty-four hours after reperfusion and later,perfusion on the affected side declined slowly and stabilized after 1 week;brain injury was also alleviated.Histopathological and immunohistochemical examinations confirmed the brain injury tissue changes.The nanoshell molecular probe carrying anti-tropomyosin 4 has potential for use in early diagnosis of cerebral ischemia/reperfusion injury and evaluating its progression.
基金supported by NSFC Major Research Plan on‘‘West-Pacific Earth System Multispheric Interactions’’to Prof.Weidong Sun(Grant No.92258303)AND Prof.Tianyu Chen(Grant No.91858105)。
文摘At the beginning of the Cenozoic,the atmospheric CO_(2)concentration increased rapidly from~2000 ppmv at 60 Ma to~4600 ppmv at 51 Ma,which is 5–10 times higher than the present value,and then continuous declined from~51 to 34 Ma.The cause of this phenomenon is still not well understood.In this study,we demonstrate that the initiation of Cenozoic west Pacific plate subduction,triggered by the hard collision in the Tibetan Plateau,occurred at approximately 51 Ma,coinciding with the tipping point.The water depths of the Pacific subduction zones are mostly below the carbonate compensation depths,while those of the Neo-Tethys were much shallower before the collision and caused far more carbonate subducting.Additionally,more volcanic ashes erupted from the west Pacific subduction zones,which consume CO_(2).The average annual west Pacific volvano eruption is 1.11 km~3,which is higher than previous estimations.The amount of annual CO_(2)absorbed by chemical weathering of additional west Pacific volcanic ashes could be comparable to the silicate weathering by the global river.We propose that the initiation of the western Pacific subduction controlled the long-term reduction of atmospheric CO_(2)concentration.
