Forty-eight male Lezhi black goat kids with similar body weight((12.09±1.70)kg)and age((60±5)d)were used to determine the effect of dietary copper(Cu),in the form of reagent grade Cu sulfate(CuSO4∙5H2O),on p...Forty-eight male Lezhi black goat kids with similar body weight((12.09±1.70)kg)and age((60±5)d)were used to determine the effect of dietary copper(Cu),in the form of reagent grade Cu sulfate(CuSO4∙5H2O),on performance,serum lipid profile,and the relative mRNA abundance of genes involved in lipid metabolism.Goat kids were stratified by body weight and randomly assigned to one of 4 treatment groups.Each treatment consisted of 12 replicate pens with each pen containing one goat kid.Treatment groups received the basal diet with no supplemental Cu(control),basal diet plus 10 mg of Cu kg^(-1)of dry matter(DM),basal diet plus 20 mg of Cu kg^(-1)of DM,or basal diet plus 30 mg of Cu kg^(-1)of DM.Goats were housed individually in pens and fed a high-concentrate pelleted diet for 60 d.Average daily gain,average daily feed intake and feed:gain of goats were not affected by dietary Cu supplementation(P>0.10).No differences were detected in serum total cholesterol,triglyceride,and high density lipoprotein cholesterol concentrations of goat kids fed with different Cu concentrations(P>0.05).However,serum low density lipoprotein cholesterol concentrations decreased linearly(P=0.01)as the concentration of dietary Cu increased.Intramuscular fat content of longissimus muscle increased(P=0.002)quadratically and liver Cu concentrations increased(P<0.001)linearly as dietary Cu concentration increased.Compared with the control,dietary supplementation of 20 mg Cu kg^(-1)DM decreased the relative mRNA abundance of fatty acid-binding protein 4(P=0.01)and lipoprotein lipase(P=0.05),and tended to decrease the relative mRNA abundance of carnitine palmitoyltransferase I(P=0.06)in longissimus muscle of goats.The relative mRNA abundance of peroxisome proliferator-activated receptor alpha(P<0.001),carnitine acetyltransferase(P=0.001),and carnitine palmitoyltransferase Ⅰ(P=0.001)were also decreased in liver by Cu supplementation.These results indicate that dietary supplementation of Cu modified lipid metabolism by increasing muscular fat and decreasing serum low density lipoprotein cholesterol,and the modification might be associated with the reduction of relative mRNA abundance of genes for oxidation of long-chain fatty acid in muscle and liver of Lezhi black goat kids.展开更多
基于气象站点实测资料、MODIS植被产品数据集LAI(leaf area index)及世界土壤数据库(HWSD)土壤数据,结合气象插值软件ANUSPLIN、土壤水分特征曲线拟合软件RETC、空间信息处理软件ArcGIS分别对气象、土壤、植被数据进行计算;使用MIKESHE...基于气象站点实测资料、MODIS植被产品数据集LAI(leaf area index)及世界土壤数据库(HWSD)土壤数据,结合气象插值软件ANUSPLIN、土壤水分特征曲线拟合软件RETC、空间信息处理软件ArcGIS分别对气象、土壤、植被数据进行计算;使用MIKESHE分布式水文模型对拉萨河流域内2008-2017年3、7月及典型年(丰水年、中水年、枯水年)生长季(3-10月)内的表层1 m土壤含水量进行模拟。结果表明,在空间分布上,拉萨河流域表层土壤含水量自西南向东北呈递减趋势,流域中部拉萨河中上游的高山地区土壤含水量相对较低;相较于中下游河谷及西部盆地地区,中部高山地区土壤水分变化更为剧烈。在时间分布上,典型年生长季内不同月份土壤含水量变化量在33%~37%;总体而言,土壤含水量年内变化大于年际变化。拉萨河谷平原、澎波盆地、流域西部念青唐古拉山脉以东的洪积宽谷、羊八井盆地及流域东北部麦地卡湿地植被覆盖度和土壤水分含量较高、变化幅度小,农业适宜性较强。展开更多
基金supported by the National Natural Science Foundation of China(31501977)the Sichuan Provincial Key R&D Project,China(22ZDYF0194)+1 种基金the Fundamental Research Funds for the Central Universities of Southwest Minzu University(2021PTJS20)the Innovation Team Development Funds for Sichuan Mutton Sheep,China(cxtd2019–14).
文摘Forty-eight male Lezhi black goat kids with similar body weight((12.09±1.70)kg)and age((60±5)d)were used to determine the effect of dietary copper(Cu),in the form of reagent grade Cu sulfate(CuSO4∙5H2O),on performance,serum lipid profile,and the relative mRNA abundance of genes involved in lipid metabolism.Goat kids were stratified by body weight and randomly assigned to one of 4 treatment groups.Each treatment consisted of 12 replicate pens with each pen containing one goat kid.Treatment groups received the basal diet with no supplemental Cu(control),basal diet plus 10 mg of Cu kg^(-1)of dry matter(DM),basal diet plus 20 mg of Cu kg^(-1)of DM,or basal diet plus 30 mg of Cu kg^(-1)of DM.Goats were housed individually in pens and fed a high-concentrate pelleted diet for 60 d.Average daily gain,average daily feed intake and feed:gain of goats were not affected by dietary Cu supplementation(P>0.10).No differences were detected in serum total cholesterol,triglyceride,and high density lipoprotein cholesterol concentrations of goat kids fed with different Cu concentrations(P>0.05).However,serum low density lipoprotein cholesterol concentrations decreased linearly(P=0.01)as the concentration of dietary Cu increased.Intramuscular fat content of longissimus muscle increased(P=0.002)quadratically and liver Cu concentrations increased(P<0.001)linearly as dietary Cu concentration increased.Compared with the control,dietary supplementation of 20 mg Cu kg^(-1)DM decreased the relative mRNA abundance of fatty acid-binding protein 4(P=0.01)and lipoprotein lipase(P=0.05),and tended to decrease the relative mRNA abundance of carnitine palmitoyltransferase I(P=0.06)in longissimus muscle of goats.The relative mRNA abundance of peroxisome proliferator-activated receptor alpha(P<0.001),carnitine acetyltransferase(P=0.001),and carnitine palmitoyltransferase Ⅰ(P=0.001)were also decreased in liver by Cu supplementation.These results indicate that dietary supplementation of Cu modified lipid metabolism by increasing muscular fat and decreasing serum low density lipoprotein cholesterol,and the modification might be associated with the reduction of relative mRNA abundance of genes for oxidation of long-chain fatty acid in muscle and liver of Lezhi black goat kids.
文摘基于气象站点实测资料、MODIS植被产品数据集LAI(leaf area index)及世界土壤数据库(HWSD)土壤数据,结合气象插值软件ANUSPLIN、土壤水分特征曲线拟合软件RETC、空间信息处理软件ArcGIS分别对气象、土壤、植被数据进行计算;使用MIKESHE分布式水文模型对拉萨河流域内2008-2017年3、7月及典型年(丰水年、中水年、枯水年)生长季(3-10月)内的表层1 m土壤含水量进行模拟。结果表明,在空间分布上,拉萨河流域表层土壤含水量自西南向东北呈递减趋势,流域中部拉萨河中上游的高山地区土壤含水量相对较低;相较于中下游河谷及西部盆地地区,中部高山地区土壤水分变化更为剧烈。在时间分布上,典型年生长季内不同月份土壤含水量变化量在33%~37%;总体而言,土壤含水量年内变化大于年际变化。拉萨河谷平原、澎波盆地、流域西部念青唐古拉山脉以东的洪积宽谷、羊八井盆地及流域东北部麦地卡湿地植被覆盖度和土壤水分含量较高、变化幅度小,农业适宜性较强。
