The fasting evolving information technology has driven the global industry into a new period of development where integration of informatization and industrialization became the focus of all countries around the world...The fasting evolving information technology has driven the global industry into a new period of development where integration of informatization and industrialization became the focus of all countries around the world,and a key trend for thousands of enterprises to follow.This now plays a decisive role on the evolution of world’s industrial development as well as the international competition.展开更多
Objective:To study the effect of Yigan capsule on the expression of high mobility group protein B1(HMGB1),nuclear factor-B(NF-κB)and receptor for advanced glycation end products(RAGE)in anti-tuberculosis drug-induced...Objective:To study the effect of Yigan capsule on the expression of high mobility group protein B1(HMGB1),nuclear factor-B(NF-κB)and receptor for advanced glycation end products(RAGE)in anti-tuberculosis drug-induced liver injury(ATB-DILI),and to explore its protective effect and mechanism on ATB-DILI,so as to provide experimental basis for the clinical application of Yigan capsule.Methods:Twenty-four rats were divided into two groups.Except for the blank group(n=6),the other 18 rats were given isoniazid(INH)+rifampicin(RFP)(50 mg/kg.d)for 4 weeks.Then 18 rats were randomly divided into three groups(model group,low dose group of Yigan capsule and high dose group of Yigan capsule)according to 6 rats in each group.The blank group and the model group were given 0.9%sodium chloride solution by intragastric administration.The low dose group of Yigan capsule was 0.468 g/kg,and the high dose group of Yigan capsule was 1.872 g/kg[1].After 4 weeks,the pathological changes of liver were observed by HE staining.The contents of ALT,AST,ALP,γ-GT and TBIL were detected.The expression of HMGB1,NF-κBp65 and RAGE protein was detected by IHC.The expression levels of HMGB1,NF-κBp65,RAGE,TNF-αand IL-1βwere detected by WB.Result:HE staining showed that the structure of the liver in the model group was disordered,the liver cells showed swelling and fusion,the number of inflammatory cells increased and accompanied by punctate necrosis,while the above pathological changes in each treatment group of Yigan capsule were significantly improved.The contents of ALT,AST,ALP,γ-GT and TBIL in the model group were higher than those in the blank group(P<0.05).The contents of ALT,AST,ALP,γ-GT and TBIL in each treatment group were significantly lower than those in the model group(P<0.05).Compared with the blank group,the expression levels of TNF-αand IL-1βin the model group were increased(P<0.05),and the expression levels of HMGB1,NF-κBp65 and RAGE were increased(P<0.05).Compared with the model group,the expression levels of TNF-αand IL-1βin each treatment group of Yigan capsule decreased(P<0.05),and the expression of HMGB1,NF-κBp65 and RAGE decreased(P<0.05).Conclusion:Yigan capsule may inhibit the secretion of inflammatory factors through HMGB1/RAGE/NF-κBp65 signaling pathway,thus protecting ATB-DILI.展开更多
The discovery of Yin Xu has turned the history of the Shang Dynasty into credible history,continuing the trend of integrating civilizations from all directions for over a thousand years,and initiating a pattern of con...The discovery of Yin Xu has turned the history of the Shang Dynasty into credible history,continuing the trend of integrating civilizations from all directions for over a thousand years,and initiating a pattern of continuous,diversified,and unified civilization for more than three thousand years.With the Yin Xu Site as the fulcrum,it constructs the spatialtemporal system of Xia-Shang archaeology and culture.展开更多
Party construction aesthetics is a valuable aesthetic resource formed in the practical exploration of rural revitalization,and it has logical compatibility with ideological and political education in terms of concepts...Party construction aesthetics is a valuable aesthetic resource formed in the practical exploration of rural revitalization,and it has logical compatibility with ideological and political education in terms of concepts,goals,tasks,etc.At present,comprehensive deepening reform has entered a deep-water zone,and some erroneous value orientations such as utilitarianism,emptiness,and liberalization are common in colleges and universities,and ideological and political education is caught in a new round of difficulties.As the main position of ideological and political education,colleges and universities should further use party construction aesthetics to strengthen and improve ideological and political education,adhere to the carrier of aesthetic education,carry out diversified integrated education,broaden the ideological and political practical teaching system,build a long-term mechanism for collaborative education of ideological and political education and party construction aesthetics,and fully integrate party construction aesthetics into ideological and political education in colleges and universities.展开更多
目的:探索LINC01060对人胃癌BGC-823细胞系生物学行为的作用。方法:生信数据分析LINC01060在胃癌中的表达情况,建立LINC01060过表达和沉默的稳转BGC-823细胞株,Real time PCR验证LINC01060的表达,CCK-8检测细胞增殖,流式细胞术检测细胞...目的:探索LINC01060对人胃癌BGC-823细胞系生物学行为的作用。方法:生信数据分析LINC01060在胃癌中的表达情况,建立LINC01060过表达和沉默的稳转BGC-823细胞株,Real time PCR验证LINC01060的表达,CCK-8检测细胞增殖,流式细胞术检测细胞凋亡,划痕实验检测细胞迁移,Transwell检测细胞侵袭,Western blot检测EMT相关蛋白E-cadherin、Vimentin、N-cadherin和Snail蛋白的表达。结果:GEO数据库发现,LINC01060在胃癌中低表达(P<0.05);Real time PCR结果显示,沉默组LINC01060的表达显著下调(P<0.05),过表达组LINC01060的表达显著上调(P<0.05);CCK-8显示,过表达组细胞增殖能力显著下降(P<0.05);流式细胞术显示,过表达组细胞凋亡比例显著上升(P<0.05);Transwell结果显示,过表达组细胞侵袭能力显著下降(P<0.05);细胞划痕显示,过表达组细胞迁移能力显著下降(P<0.05);Western blot显示,过表达组E-cadherin蛋白表达水平显著上调(P<0.05),Vimentin、N-cadherin、Snail蛋白表达水平显著下调(P<0.05);以上实验沉默组均相反。结论:LINC01060在人胃癌BGC-823细胞中发挥促进凋亡,抑制增殖、侵袭、迁移和EMT的作用。展开更多
In the evening of October 17, 2012, the vessel Hangjun 12 of Changhang Wuhan Engineering Bureau was struck by lightning when working in Malaysia red ridge channel. In the lightning accident, the weather instrument of ...In the evening of October 17, 2012, the vessel Hangjun 12 of Changhang Wuhan Engineering Bureau was struck by lightning when working in Malaysia red ridge channel. In the lightning accident, the weather instrument of the ship was destroyed by the lightning to the ground, and the electronic information equipment in the vessel was influenced by induction lightning, which had damaged the electronic information system and elec- trical equipment. According to the Code for Design Protection of Structures against Lightning （ GB 50057-2010）, this lightning accident suffered by Hangjun 12 was analyzed theoretically from aspects of the protection scope of the two lightning reds, main measures against lightning electromag- netic impulse, and specific reasons for the lightning accident. Finally, some measures to prevent the ship from being struck by lightning were put forward, such as improving countermeasures against direct lightning flash, enhancing equipotential bonding and shielding measures and so forth.展开更多
文摘The fasting evolving information technology has driven the global industry into a new period of development where integration of informatization and industrialization became the focus of all countries around the world,and a key trend for thousands of enterprises to follow.This now plays a decisive role on the evolution of world’s industrial development as well as the international competition.
基金Scientific Research Project of Heilongjiang Provincial Education Department(No.12531608)。
文摘Objective:To study the effect of Yigan capsule on the expression of high mobility group protein B1(HMGB1),nuclear factor-B(NF-κB)and receptor for advanced glycation end products(RAGE)in anti-tuberculosis drug-induced liver injury(ATB-DILI),and to explore its protective effect and mechanism on ATB-DILI,so as to provide experimental basis for the clinical application of Yigan capsule.Methods:Twenty-four rats were divided into two groups.Except for the blank group(n=6),the other 18 rats were given isoniazid(INH)+rifampicin(RFP)(50 mg/kg.d)for 4 weeks.Then 18 rats were randomly divided into three groups(model group,low dose group of Yigan capsule and high dose group of Yigan capsule)according to 6 rats in each group.The blank group and the model group were given 0.9%sodium chloride solution by intragastric administration.The low dose group of Yigan capsule was 0.468 g/kg,and the high dose group of Yigan capsule was 1.872 g/kg[1].After 4 weeks,the pathological changes of liver were observed by HE staining.The contents of ALT,AST,ALP,γ-GT and TBIL were detected.The expression of HMGB1,NF-κBp65 and RAGE protein was detected by IHC.The expression levels of HMGB1,NF-κBp65,RAGE,TNF-αand IL-1βwere detected by WB.Result:HE staining showed that the structure of the liver in the model group was disordered,the liver cells showed swelling and fusion,the number of inflammatory cells increased and accompanied by punctate necrosis,while the above pathological changes in each treatment group of Yigan capsule were significantly improved.The contents of ALT,AST,ALP,γ-GT and TBIL in the model group were higher than those in the blank group(P<0.05).The contents of ALT,AST,ALP,γ-GT and TBIL in each treatment group were significantly lower than those in the model group(P<0.05).Compared with the blank group,the expression levels of TNF-αand IL-1βin the model group were increased(P<0.05),and the expression levels of HMGB1,NF-κBp65 and RAGE were increased(P<0.05).Compared with the model group,the expression levels of TNF-αand IL-1βin each treatment group of Yigan capsule decreased(P<0.05),and the expression of HMGB1,NF-κBp65 and RAGE decreased(P<0.05).Conclusion:Yigan capsule may inhibit the secretion of inflammatory factors through HMGB1/RAGE/NF-κBp65 signaling pathway,thus protecting ATB-DILI.
文摘The discovery of Yin Xu has turned the history of the Shang Dynasty into credible history,continuing the trend of integrating civilizations from all directions for over a thousand years,and initiating a pattern of continuous,diversified,and unified civilization for more than three thousand years.With the Yin Xu Site as the fulcrum,it constructs the spatialtemporal system of Xia-Shang archaeology and culture.
基金supported by the National Social Science Foundation of China under the General Program“Study on the Mechanism and Path of Creative Transformation of China’s Traditional Aesthetic Education Ideas in the New Development Stage”,Project No.21BKS083Key Project of 2022 Research on Higher Education Reform and Development of Nanjing University of Finance and Economics,“Thoughts and Paths on Empowering High-level University Construction with Party Building Aesthetics”,Project No.GJGF202203.
文摘Party construction aesthetics is a valuable aesthetic resource formed in the practical exploration of rural revitalization,and it has logical compatibility with ideological and political education in terms of concepts,goals,tasks,etc.At present,comprehensive deepening reform has entered a deep-water zone,and some erroneous value orientations such as utilitarianism,emptiness,and liberalization are common in colleges and universities,and ideological and political education is caught in a new round of difficulties.As the main position of ideological and political education,colleges and universities should further use party construction aesthetics to strengthen and improve ideological and political education,adhere to the carrier of aesthetic education,carry out diversified integrated education,broaden the ideological and political practical teaching system,build a long-term mechanism for collaborative education of ideological and political education and party construction aesthetics,and fully integrate party construction aesthetics into ideological and political education in colleges and universities.
文摘目的:探索LINC01060对人胃癌BGC-823细胞系生物学行为的作用。方法:生信数据分析LINC01060在胃癌中的表达情况,建立LINC01060过表达和沉默的稳转BGC-823细胞株,Real time PCR验证LINC01060的表达,CCK-8检测细胞增殖,流式细胞术检测细胞凋亡,划痕实验检测细胞迁移,Transwell检测细胞侵袭,Western blot检测EMT相关蛋白E-cadherin、Vimentin、N-cadherin和Snail蛋白的表达。结果:GEO数据库发现,LINC01060在胃癌中低表达(P<0.05);Real time PCR结果显示,沉默组LINC01060的表达显著下调(P<0.05),过表达组LINC01060的表达显著上调(P<0.05);CCK-8显示,过表达组细胞增殖能力显著下降(P<0.05);流式细胞术显示,过表达组细胞凋亡比例显著上升(P<0.05);Transwell结果显示,过表达组细胞侵袭能力显著下降(P<0.05);细胞划痕显示,过表达组细胞迁移能力显著下降(P<0.05);Western blot显示,过表达组E-cadherin蛋白表达水平显著上调(P<0.05),Vimentin、N-cadherin、Snail蛋白表达水平显著下调(P<0.05);以上实验沉默组均相反。结论:LINC01060在人胃癌BGC-823细胞中发挥促进凋亡,抑制增殖、侵袭、迁移和EMT的作用。
文摘In the evening of October 17, 2012, the vessel Hangjun 12 of Changhang Wuhan Engineering Bureau was struck by lightning when working in Malaysia red ridge channel. In the lightning accident, the weather instrument of the ship was destroyed by the lightning to the ground, and the electronic information equipment in the vessel was influenced by induction lightning, which had damaged the electronic information system and elec- trical equipment. According to the Code for Design Protection of Structures against Lightning （ GB 50057-2010）, this lightning accident suffered by Hangjun 12 was analyzed theoretically from aspects of the protection scope of the two lightning reds, main measures against lightning electromag- netic impulse, and specific reasons for the lightning accident. Finally, some measures to prevent the ship from being struck by lightning were put forward, such as improving countermeasures against direct lightning flash, enhancing equipotential bonding and shielding measures and so forth.
