Our previous studies have successfully grafted biotin and galactose onto chitosan(CS)and synthesized biotin modified galactosylated chitosan(Bio-GC).The optimum N/P ratio of Bio-GC and plasmid DNA was 3:1.At this N/P ...Our previous studies have successfully grafted biotin and galactose onto chitosan(CS)and synthesized biotin modified galactosylated chitosan(Bio-GC).The optimum N/P ratio of Bio-GC and plasmid DNA was 3:1.At this N/P ratio,the transfection efficiency in the hepatoma cells was the highest with a slow release effect.Bio-GC nanomaterials exhibit the protective effect of preventing the gene from nuclease degradation,and can target the transfection into hepatoma cells by combination with galactose and biotin receptors.The transfection rate was inhibited by the competition of galactose and biotin.Bio-GC nanomaterials were imported into cells’cytoplasm by their receptors,followed by the imported exogenous gene transfected into the cells.Bio-GC nanomaterials can also cause inhibitory activity in the hepatoma cells in the model of orthotopic liver transplantation in mice,by carrying the gene through the blood to the hepatoma tissue.Taken together,bio-GC nanomaterials act as gene vectors with the activity of protecting the gene from DNase degradation,improving the rate of transfection in hepatoma cells,and transporting the gene into the cytoplasm in vitro and in vivo.Therefore,they are efficient hepatoma-targeting gene carriers.展开更多
In recent years, lakes on the Qinghai-Tibet Plateau have become more responsive to climate change. In September 2011, Zonag Lake in Hoh Xil experienced sudden drainage, the water eventually flowed into Yanhu Lake, whi...In recent years, lakes on the Qinghai-Tibet Plateau have become more responsive to climate change. In September 2011, Zonag Lake in Hoh Xil experienced sudden drainage, the water eventually flowed into Yanhu Lake, which caused Yanhu Lake to continue to expand. The potential collapse of Yanhu Lake could directly threaten the operational safety of the adjacent Qinghai-Tibet Highway, Qinghai-Tibet Railway. To explore the implications of expanding lakes on the surrounding permafrost, we selected Hoh Xil Yanhu Lake on the Qinghai-Tibet Plateau to study the effect of lake expansion on permafrost degradation. The permafrost degradation in the Yanhu Lake basin from October 2017 to December 2022 was inverted using Sentinel-1 satellite image data and small baseline subset interferometry synthetic aperture radar(SBAS-In SAR) technology. Additionally, permafrost degradation from February 2007 and February 2010 was analyzed using advanced land observing satellite phased array-type L-band synthetic aperture radar(ALOS PALSAR) satellite images and differential interferometric synthetic aperture radar(D-In SAR) technique. The results showed that the permafrost around Yanhu Lake experienced accelerated degradation. Prior to the expansion of Yanhu Lake, the average annual deformation rate along the line of sight(LOS) direction was 6.7 mm/yr. After the expansion, the rate increased to 20.9 mm/yr. The integration of spatial-temporal distribution maps of surface subsidence, Wudaoliang borehole geothermal data, meteorological data, Yanhu Lake surface area changes, and water level changes supports the assertion that the intensified permafrost degradation could be attributed to lake expansion rather than the rising air temperature. Furthermore, permafrost degradation around Yanhu Lake could impact vital infrastructure such as the adjacent Qinghai-Tibet Highway and Qinghai-Tibet Railway.展开更多
目的:通过对国内外代谢组学在疾病诊断中的相关研究进行文献计量及可视化分析,探索其研究现状与热点,以期为该领域的进一步研究与发展提供一定的参考。方法:通过检索近6年中国知网数据库和Web of Science核心合集数据库中的相关文献,运...目的:通过对国内外代谢组学在疾病诊断中的相关研究进行文献计量及可视化分析,探索其研究现状与热点,以期为该领域的进一步研究与发展提供一定的参考。方法:通过检索近6年中国知网数据库和Web of Science核心合集数据库中的相关文献,运用VOSviewer和COOC软件对国家、作者、机构、被引频次和关键词进行共现分析。结果:在CNKI和WOS数据库中,发文量最多的国家是中国;发文量最多的机构为河南中医药大学和中国科学院;关键词生物标志物、质谱分析、蛋白质组学、核磁共振、尿液、液相色谱质谱联用等出现频次较高。结论:近6年,国内外对代谢组学在疾病诊断中的研究热度不断上升,研究热点为利用代谢组学、蛋白质组学、糖组学等多组学整合技术与菌群相结合寻找不同疾病患者与健康人之间的生物标志物、疾病发病机制以及不同干预措施作用于机体起效的代谢通路,研究方向也逐渐从恶性肿瘤疾病转向对同一疾病不同证型。展开更多
As significant evidence of permafrost degradation,thermokarst lakes play an important role in the permafrost regions by regulating hydrology,ecology,and biogeochemistry.In the Sources Area of the Yellow River(SAYR),pe...As significant evidence of permafrost degradation,thermokarst lakes play an important role in the permafrost regions by regulating hydrology,ecology,and biogeochemistry.In the Sources Area of the Yellow River(SAYR),permafrost degradation has accelerated since the 1980s,and numerous thermokarst lakes have been discovered.In this paper,we use Sentinel-2 images to extract thermokarst lake boundaries and perform a regional-scale study on their geometry across the permafrost region in the SAYR.We also explored the spatiotemporal variations and potential drivers from the perspectives of the permafrost,climate,terrain and vegetation conditions.The results showed that there were 47,518 thermokarst lakes in 2021 with a total area of 190.22×106 m^(2),with an average size of 4,003.3 m^(2).The 44,928 ponds(≤10,000 m^(2))predominated the whole lake number(94.1%)but contributed to a small portion of the total lake area(28.8%).With 2,590 features(5.9%),small-sized(10,000 to 100,000 m^(2))and large-sized lakes(>100,000 m^(2))constituted up to 71.2%of the total lake area.Thermokarst lakes developed more significantly in warm permafrost regions than in cold permafrost areas;74.1%of lakes with a total area of 119.6×106 m^(2)(62.9%),were distributed in warm permafrost regions.Most thermokarst lakes were likely to develop within the elevation range of 4,500~4,800 m,on flat terrain(slope<10°),on SE and S aspects and in alpine meadow areas.The thermokarst lakes in the study region experienced significant shrinkage between 1990 and 2021,characterized by obvious lake drainage;the lake numbers decreased by 5418(56.1%),with a decreasing area of 58.63×106 m^(2)(49.0%).This shrinkage of the thermokarst lake area was attributable mainly to the intensified degradation of rich-ice permafrost thawing arising from continued climate warming,despite the wetting climatic trend.展开更多
基金Funded by the Scientific Research Project of Shanghai Municipal Health Commission(No.201940430)。
文摘Our previous studies have successfully grafted biotin and galactose onto chitosan(CS)and synthesized biotin modified galactosylated chitosan(Bio-GC).The optimum N/P ratio of Bio-GC and plasmid DNA was 3:1.At this N/P ratio,the transfection efficiency in the hepatoma cells was the highest with a slow release effect.Bio-GC nanomaterials exhibit the protective effect of preventing the gene from nuclease degradation,and can target the transfection into hepatoma cells by combination with galactose and biotin receptors.The transfection rate was inhibited by the competition of galactose and biotin.Bio-GC nanomaterials were imported into cells’cytoplasm by their receptors,followed by the imported exogenous gene transfected into the cells.Bio-GC nanomaterials can also cause inhibitory activity in the hepatoma cells in the model of orthotopic liver transplantation in mice,by carrying the gene through the blood to the hepatoma tissue.Taken together,bio-GC nanomaterials act as gene vectors with the activity of protecting the gene from DNase degradation,improving the rate of transfection in hepatoma cells,and transporting the gene into the cytoplasm in vitro and in vivo.Therefore,they are efficient hepatoma-targeting gene carriers.
基金supported by the Natural Science Foundation of Qinghai Province, China (No.2021-ZJ940Q)。
文摘In recent years, lakes on the Qinghai-Tibet Plateau have become more responsive to climate change. In September 2011, Zonag Lake in Hoh Xil experienced sudden drainage, the water eventually flowed into Yanhu Lake, which caused Yanhu Lake to continue to expand. The potential collapse of Yanhu Lake could directly threaten the operational safety of the adjacent Qinghai-Tibet Highway, Qinghai-Tibet Railway. To explore the implications of expanding lakes on the surrounding permafrost, we selected Hoh Xil Yanhu Lake on the Qinghai-Tibet Plateau to study the effect of lake expansion on permafrost degradation. The permafrost degradation in the Yanhu Lake basin from October 2017 to December 2022 was inverted using Sentinel-1 satellite image data and small baseline subset interferometry synthetic aperture radar(SBAS-In SAR) technology. Additionally, permafrost degradation from February 2007 and February 2010 was analyzed using advanced land observing satellite phased array-type L-band synthetic aperture radar(ALOS PALSAR) satellite images and differential interferometric synthetic aperture radar(D-In SAR) technique. The results showed that the permafrost around Yanhu Lake experienced accelerated degradation. Prior to the expansion of Yanhu Lake, the average annual deformation rate along the line of sight(LOS) direction was 6.7 mm/yr. After the expansion, the rate increased to 20.9 mm/yr. The integration of spatial-temporal distribution maps of surface subsidence, Wudaoliang borehole geothermal data, meteorological data, Yanhu Lake surface area changes, and water level changes supports the assertion that the intensified permafrost degradation could be attributed to lake expansion rather than the rising air temperature. Furthermore, permafrost degradation around Yanhu Lake could impact vital infrastructure such as the adjacent Qinghai-Tibet Highway and Qinghai-Tibet Railway.
文摘目的:通过对国内外代谢组学在疾病诊断中的相关研究进行文献计量及可视化分析,探索其研究现状与热点,以期为该领域的进一步研究与发展提供一定的参考。方法:通过检索近6年中国知网数据库和Web of Science核心合集数据库中的相关文献,运用VOSviewer和COOC软件对国家、作者、机构、被引频次和关键词进行共现分析。结果:在CNKI和WOS数据库中,发文量最多的国家是中国;发文量最多的机构为河南中医药大学和中国科学院;关键词生物标志物、质谱分析、蛋白质组学、核磁共振、尿液、液相色谱质谱联用等出现频次较高。结论:近6年,国内外对代谢组学在疾病诊断中的研究热度不断上升,研究热点为利用代谢组学、蛋白质组学、糖组学等多组学整合技术与菌群相结合寻找不同疾病患者与健康人之间的生物标志物、疾病发病机制以及不同干预措施作用于机体起效的代谢通路,研究方向也逐渐从恶性肿瘤疾病转向对同一疾病不同证型。
基金supported by the Natural Science Foundation of Qinghai Province,China(No.2021-ZJ940Q)the Open Project of State Key Laboratory of Plateau Ecology and Agriculture,Qinghai University(No.2022-ZZ-02)。
文摘As significant evidence of permafrost degradation,thermokarst lakes play an important role in the permafrost regions by regulating hydrology,ecology,and biogeochemistry.In the Sources Area of the Yellow River(SAYR),permafrost degradation has accelerated since the 1980s,and numerous thermokarst lakes have been discovered.In this paper,we use Sentinel-2 images to extract thermokarst lake boundaries and perform a regional-scale study on their geometry across the permafrost region in the SAYR.We also explored the spatiotemporal variations and potential drivers from the perspectives of the permafrost,climate,terrain and vegetation conditions.The results showed that there were 47,518 thermokarst lakes in 2021 with a total area of 190.22×106 m^(2),with an average size of 4,003.3 m^(2).The 44,928 ponds(≤10,000 m^(2))predominated the whole lake number(94.1%)but contributed to a small portion of the total lake area(28.8%).With 2,590 features(5.9%),small-sized(10,000 to 100,000 m^(2))and large-sized lakes(>100,000 m^(2))constituted up to 71.2%of the total lake area.Thermokarst lakes developed more significantly in warm permafrost regions than in cold permafrost areas;74.1%of lakes with a total area of 119.6×106 m^(2)(62.9%),were distributed in warm permafrost regions.Most thermokarst lakes were likely to develop within the elevation range of 4,500~4,800 m,on flat terrain(slope<10°),on SE and S aspects and in alpine meadow areas.The thermokarst lakes in the study region experienced significant shrinkage between 1990 and 2021,characterized by obvious lake drainage;the lake numbers decreased by 5418(56.1%),with a decreasing area of 58.63×106 m^(2)(49.0%).This shrinkage of the thermokarst lake area was attributable mainly to the intensified degradation of rich-ice permafrost thawing arising from continued climate warming,despite the wetting climatic trend.
