Greenhouse vegetable production has been characterized by high agricultural inputs, high temperatures, and high cropping indexes. As an intensive form of agriculture, nutrient cycling induced by microbial activities i...Greenhouse vegetable production has been characterized by high agricultural inputs, high temperatures, and high cropping indexes. As an intensive form of agriculture, nutrient cycling induced by microbial activities in the greenhouses is relatively different from open fields in the same region. However, the responses of soil microbial biomass carbon(MBC) and nitrogen(MBN), enzyme activities, microbial community composition, and yield to organic amendment are not well understood. Therefore, a 5-year greenhouse tomato(Solanum lycopersicum Mill.)-cucumber(Cucumis sativus L.) rotation experiment was conducted. The field experiment comprised 5 treatments: 4/4 CN(CN, nitrogen in chemical fertilizer), 3/4 CN+1/4 MN(MN, nitrogen in pig manure), 2/4 CN+2/4 MN, 2/4 CN+1/4 MN+1/4 SN(SN, nitrogen in corn straw) and 2/4 CN+2/4 SN. The amounts of nitrogen(N), phosphorus(P_2O_5), and potassium(K_2O) were equal in the five treatments. Starting with the fourth growing season, the optimal yield was obtained from soil treated with straw. MBC, MBN, phospholipid fatty acid(PLFA) profiles, and enzyme activities were significantly changed by 5 years of substitution with organic amendments. Redundancy analysis showed that MBC accounts for 89.5 and 52.3% of the total enzyme activity and total community variability, respectively. The activities of phosphomonoesterase, N-acetyl-glucosaminidase, and urease, and the relative abundances of fungi, actinomycetes, and Gram-negative bacteria were significantly and positively related to vegetable yields. Considering the effects of organic amendments on soil microbial characteristics and vegetable yield, 2/4 CN+1/4 MN+1/4 SN can improve soil quality and maintain sustainable high yield in greenhouse vegetable production.展开更多
Partial substitution of chemical fertilizers by organic amendments is adopted widely for promoting the availability of soil phosphorus(P)in agricultural production.However,few studies have comprehensively evaluated th...Partial substitution of chemical fertilizers by organic amendments is adopted widely for promoting the availability of soil phosphorus(P)in agricultural production.However,few studies have comprehensively evaluated the effects of longterm organic substitution on soil P availability and microbial activity in greenhouse vegetable fields.A 10-year(2009–2019)field experiment was carried out to investigate the impacts of organic fertilizer substitution on soil P pools,phosphatase activities and the microbial community,and identify factors that regulate these soil P transformation characteristics.Four treatments included 100%chemical N fertilizer(4 CN),50%substitution of chemical N by manure(2 CN+2 MN),straw(2 CN+2 SN),and combined manure with straw(2 CN+1 MN+1 SN).Compared with the 4 CN treatment,organic substitution treatments increased celery and tomato yields by 6.9-13.8%and 8.6-18.1%,respectively,with the highest yields being in the 2 CN+1 MN+1 SN treatment.After 10 years of fertilization,organic substitution treatments reduced total P and inorganic P accumulation,increased the concentrations of available P,organic P,and microbial biomass P,and promoted phosphatase activities(alkaline and acid phosphomonoesterase,phosphodiesterase,and phytase)and microbial growth in comparison with the 4 CN treatment.Further,organic substitution treatments significantly increased soil C/P,and the partial least squares path model(PLS-PM)revealed that the soil C/P ratio directly and significantly affected phosphatase activities and the microbial biomass and positively influenced soil P pools and vegetable yield.Partial least squares(PLS)regression demonstrated that arbuscular mycorrhizal fungi positively affected phosphatase activities.Our results suggest that organic fertilizer substitution can promote soil P transformation and availability.Combining manure with straw was more effective than applying these materials separately for developing sustainable P management practices.展开更多
Soil aggregation,microbial community,and functions(i.e.,extracellular enzyme activities;EEAs)are critical factors affecting soil C dynamics and nutrient cycling.We assessed soil aggregate distribution,stability,nutrie...Soil aggregation,microbial community,and functions(i.e.,extracellular enzyme activities;EEAs)are critical factors affecting soil C dynamics and nutrient cycling.We assessed soil aggregate distribution,stability,nutrients,and microbial characteristics within>2,0.25-2,0.053-0.25,and<0.053 mm aggregates,based on an eight-year field experiment in a greenhouse vegetable field in China.The field experiment includes four treatments:100%N fertilizer(CF),50%substitution of N frtilizer with manure(M),straw(S),and manure plus straw(MS).The amounts of nutrient(N,P20,and K20)input were equal in each treatment.Results showed higher values of mean weight diameter in organic amended soils(M,MS,and S,2.43-2.97)vs.CF-amended soils(1.99).Relative to CF treatment,organic amendments had positive effects on nutrient(i.e.,available N,P,and soil organic C(SOC))conditions,microbial(e.g,bacterial and fungal)growth,and EEAs in the>0.053 mm aggregates,but not in the<0.053 mm aggregates.The 0.25-0.053 mm aggregates exhibited better nutrient conditions and hydrolytic activity,while the<0.053 mm aggregates had poor nutrient conditions and higher oxidative activity among aggregates,per SOC,available N,available P,and a series of enzyme activities.These results indicated that the 0.25-0.053 mm(<0.053 mm)aggregates provide suitable microhabitats for hydrolytic(oxidative)activity.Interestingly,we found that hydrolytic and oxidative activities were mainly impacted by fertilization(58.5%,P<0.01)and aggregate fractions(50.5%,P<0.01),respectively.The hydrolytic and oxidative activities were significantly(P<0.01)associated with nutrients(SOC and available N)and pH,electrical conductivity,respectively.Furthermore,SOC,available N,and available P closely(P<0.05)afected microbial communities within>0.25,0.25-0.053,and<0.053 mm aggregates,respectively.These findings provide several insights into microbial characteristics within aggregates under dfferent frilization modes in the greenhouse vegetable production system in China.展开更多
Climate change influences both ecosystems and ecosystem services.The impacts of climate change on ecosystems and ecosystem services have been separately documented.However,it is less well known how ecosystem changes d...Climate change influences both ecosystems and ecosystem services.The impacts of climate change on ecosystems and ecosystem services have been separately documented.However,it is less well known how ecosystem changes driven by climate change will influence ecosystem services,especially in climate-sensitive regions.Here,we analyzed future climate trends between 2040 and 2100 under four Shared Socioeconomic Pathway(SSP) scenarios(SSP1-2.6,SSP2-4.5,SSP3-7.0,and SSP5-8.5) from the Coupled Model Intercomparison Project 6(CMIP6).We quantified their impacts on ecosystems patterns and on the ecosystem service of sandstorm prevention on the Qinghai-Tibet Plateau(QTP),one of the most climate-sensitive regions in the world,using Random Forest model(RF) and Revised Wind Erosion Equation(RWEQ).Strong warming(0.04℃/yr) and wetting(0.65 mm/yr) trends were projected from 2015 to 2100.Under these trends,there will be increased interspersion in the pattern of grassland and sparse vegetation with meadow and swamp vegetation,although their overall area will remain similar,while the areas of shrub and needleleaved forest classes will increase and move toward higher altitudes.Driven by the changes in ecosystem patterns caused by climate change indirectly,grassland will play an irreplaceable role in providing sandstorm prevention services,and sandstorm prevention services will increase gradually from 2040 to 2100(1.059-1.070 billion tons) on the QTP.However,some areas show a risk of deterioration in the future and these should be the focus of ecological rehabilitation.Our research helps to understand the cascading relationship among climate change,ecosystem patterns and ecosystem services,which provides important spatio-temporal information for future ecosystem service management.展开更多
Knowledge of the stability of soil organic C(SOC)is vital for assessing SOC dynamics and cycling in agroecosystems.Studies have documented the regulatory effect of fertilization on SOC stability in bulk soils.However,...Knowledge of the stability of soil organic C(SOC)is vital for assessing SOC dynamics and cycling in agroecosystems.Studies have documented the regulatory effect of fertilization on SOC stability in bulk soils.However,how fertilization alters organic C stability at the aggregate scale in agroecosystems remains largely unclear.This study aimed to appraise the changes of organic C stability within soil aggregates after eight years of fertilization(chemical vs.organic fertilization)in a greenhouse vegetable field in Tianjin,China.Changes in the stability of organic C in soil aggregates were evaluated by four methods,i.e.,the modified Walkley-Black method(chemical method),13C NMR spectroscopy(spectroscopic method),extracellular enzyme assay(biological method),and thermogravimetric analysis(thermogravimetric method).The aggregates were isolated and separated by a wet-sieving method into four fractions:large macroaggregates(>2 mm),small macroaggregates(0.25–2 mm),microaggregates(0.053–0.25 mm),and silt/clay fractions(<0.053 mm).The results showed that organic amendments increased the organic C content and reduced the chemical,spectroscopic,thermogravimetric,and biological stability of organic C within soil aggregates relative to chemical fertilization alone.Within soil aggregates,the content of organic C was the highest in microaggregates and decreased in the order microaggregates>macroaggregates>silt/clay fractions.Meanwhile,organic C spectroscopic,thermogravimetric,and biological stability were the highest in silt/clay fractions,followed by macroaggregates and microaggregates.Moreover,the modified Walkley-Black method was not suitable for interpreting organic C stability at the aggregate scale due to the weak correlation between organic C chemical properties and other stability characteristics within the soil aggregates.These findings provide scientific insights at the aggregate scale into the changes of organic C properties under fertilization in greenhouse vegetable fields in China.展开更多
基金supported by the National Key Research and Development Program of China (2016YFD0201001)the earmarked fund for China Agriculture Research System (CARS-23-B02)the Key Research and Development Program of Shandong Province,China (2017CXGC0206)
文摘Greenhouse vegetable production has been characterized by high agricultural inputs, high temperatures, and high cropping indexes. As an intensive form of agriculture, nutrient cycling induced by microbial activities in the greenhouses is relatively different from open fields in the same region. However, the responses of soil microbial biomass carbon(MBC) and nitrogen(MBN), enzyme activities, microbial community composition, and yield to organic amendment are not well understood. Therefore, a 5-year greenhouse tomato(Solanum lycopersicum Mill.)-cucumber(Cucumis sativus L.) rotation experiment was conducted. The field experiment comprised 5 treatments: 4/4 CN(CN, nitrogen in chemical fertilizer), 3/4 CN+1/4 MN(MN, nitrogen in pig manure), 2/4 CN+2/4 MN, 2/4 CN+1/4 MN+1/4 SN(SN, nitrogen in corn straw) and 2/4 CN+2/4 SN. The amounts of nitrogen(N), phosphorus(P_2O_5), and potassium(K_2O) were equal in the five treatments. Starting with the fourth growing season, the optimal yield was obtained from soil treated with straw. MBC, MBN, phospholipid fatty acid(PLFA) profiles, and enzyme activities were significantly changed by 5 years of substitution with organic amendments. Redundancy analysis showed that MBC accounts for 89.5 and 52.3% of the total enzyme activity and total community variability, respectively. The activities of phosphomonoesterase, N-acetyl-glucosaminidase, and urease, and the relative abundances of fungi, actinomycetes, and Gram-negative bacteria were significantly and positively related to vegetable yields. Considering the effects of organic amendments on soil microbial characteristics and vegetable yield, 2/4 CN+1/4 MN+1/4 SN can improve soil quality and maintain sustainable high yield in greenhouse vegetable production.
基金supported by the China Agriculture Research System of MOF and MARA(CARS-23-B04)the National Key Research and Development Program of China(2016YFD0201001)。
文摘Partial substitution of chemical fertilizers by organic amendments is adopted widely for promoting the availability of soil phosphorus(P)in agricultural production.However,few studies have comprehensively evaluated the effects of longterm organic substitution on soil P availability and microbial activity in greenhouse vegetable fields.A 10-year(2009–2019)field experiment was carried out to investigate the impacts of organic fertilizer substitution on soil P pools,phosphatase activities and the microbial community,and identify factors that regulate these soil P transformation characteristics.Four treatments included 100%chemical N fertilizer(4 CN),50%substitution of chemical N by manure(2 CN+2 MN),straw(2 CN+2 SN),and combined manure with straw(2 CN+1 MN+1 SN).Compared with the 4 CN treatment,organic substitution treatments increased celery and tomato yields by 6.9-13.8%and 8.6-18.1%,respectively,with the highest yields being in the 2 CN+1 MN+1 SN treatment.After 10 years of fertilization,organic substitution treatments reduced total P and inorganic P accumulation,increased the concentrations of available P,organic P,and microbial biomass P,and promoted phosphatase activities(alkaline and acid phosphomonoesterase,phosphodiesterase,and phytase)and microbial growth in comparison with the 4 CN treatment.Further,organic substitution treatments significantly increased soil C/P,and the partial least squares path model(PLS-PM)revealed that the soil C/P ratio directly and significantly affected phosphatase activities and the microbial biomass and positively influenced soil P pools and vegetable yield.Partial least squares(PLS)regression demonstrated that arbuscular mycorrhizal fungi positively affected phosphatase activities.Our results suggest that organic fertilizer substitution can promote soil P transformation and availability.Combining manure with straw was more effective than applying these materials separately for developing sustainable P management practices.
基金Fund for China Agriculture Research 3ystem(CAR3-23-B02)the National Key Research and Development Program of China(2016YFD0201001)the Key Research and Development Program of Shandong Province,China(2017CXGC0206).
文摘Soil aggregation,microbial community,and functions(i.e.,extracellular enzyme activities;EEAs)are critical factors affecting soil C dynamics and nutrient cycling.We assessed soil aggregate distribution,stability,nutrients,and microbial characteristics within>2,0.25-2,0.053-0.25,and<0.053 mm aggregates,based on an eight-year field experiment in a greenhouse vegetable field in China.The field experiment includes four treatments:100%N fertilizer(CF),50%substitution of N frtilizer with manure(M),straw(S),and manure plus straw(MS).The amounts of nutrient(N,P20,and K20)input were equal in each treatment.Results showed higher values of mean weight diameter in organic amended soils(M,MS,and S,2.43-2.97)vs.CF-amended soils(1.99).Relative to CF treatment,organic amendments had positive effects on nutrient(i.e.,available N,P,and soil organic C(SOC))conditions,microbial(e.g,bacterial and fungal)growth,and EEAs in the>0.053 mm aggregates,but not in the<0.053 mm aggregates.The 0.25-0.053 mm aggregates exhibited better nutrient conditions and hydrolytic activity,while the<0.053 mm aggregates had poor nutrient conditions and higher oxidative activity among aggregates,per SOC,available N,available P,and a series of enzyme activities.These results indicated that the 0.25-0.053 mm(<0.053 mm)aggregates provide suitable microhabitats for hydrolytic(oxidative)activity.Interestingly,we found that hydrolytic and oxidative activities were mainly impacted by fertilization(58.5%,P<0.01)and aggregate fractions(50.5%,P<0.01),respectively.The hydrolytic and oxidative activities were significantly(P<0.01)associated with nutrients(SOC and available N)and pH,electrical conductivity,respectively.Furthermore,SOC,available N,and available P closely(P<0.05)afected microbial communities within>0.25,0.25-0.053,and<0.053 mm aggregates,respectively.These findings provide several insights into microbial characteristics within aggregates under dfferent frilization modes in the greenhouse vegetable production system in China.
基金supported by the Second Tibetan Plateau Scientific Expedition and Research Program (STEP) (Grant No.2019QZKK0307)。
文摘Climate change influences both ecosystems and ecosystem services.The impacts of climate change on ecosystems and ecosystem services have been separately documented.However,it is less well known how ecosystem changes driven by climate change will influence ecosystem services,especially in climate-sensitive regions.Here,we analyzed future climate trends between 2040 and 2100 under four Shared Socioeconomic Pathway(SSP) scenarios(SSP1-2.6,SSP2-4.5,SSP3-7.0,and SSP5-8.5) from the Coupled Model Intercomparison Project 6(CMIP6).We quantified their impacts on ecosystems patterns and on the ecosystem service of sandstorm prevention on the Qinghai-Tibet Plateau(QTP),one of the most climate-sensitive regions in the world,using Random Forest model(RF) and Revised Wind Erosion Equation(RWEQ).Strong warming(0.04℃/yr) and wetting(0.65 mm/yr) trends were projected from 2015 to 2100.Under these trends,there will be increased interspersion in the pattern of grassland and sparse vegetation with meadow and swamp vegetation,although their overall area will remain similar,while the areas of shrub and needleleaved forest classes will increase and move toward higher altitudes.Driven by the changes in ecosystem patterns caused by climate change indirectly,grassland will play an irreplaceable role in providing sandstorm prevention services,and sandstorm prevention services will increase gradually from 2040 to 2100(1.059-1.070 billion tons) on the QTP.However,some areas show a risk of deterioration in the future and these should be the focus of ecological rehabilitation.Our research helps to understand the cascading relationship among climate change,ecosystem patterns and ecosystem services,which provides important spatio-temporal information for future ecosystem service management.
基金The authors sincerely acknowledge the financial support provided by the China Agriculture Research System of MOF and MARA(CARS-23-B02)the National Key Research and Development Program of China(2016YFD0201001)the scientific research projects for talents introduce in Hebei Agricultural University(YJ2020054).
文摘Knowledge of the stability of soil organic C(SOC)is vital for assessing SOC dynamics and cycling in agroecosystems.Studies have documented the regulatory effect of fertilization on SOC stability in bulk soils.However,how fertilization alters organic C stability at the aggregate scale in agroecosystems remains largely unclear.This study aimed to appraise the changes of organic C stability within soil aggregates after eight years of fertilization(chemical vs.organic fertilization)in a greenhouse vegetable field in Tianjin,China.Changes in the stability of organic C in soil aggregates were evaluated by four methods,i.e.,the modified Walkley-Black method(chemical method),13C NMR spectroscopy(spectroscopic method),extracellular enzyme assay(biological method),and thermogravimetric analysis(thermogravimetric method).The aggregates were isolated and separated by a wet-sieving method into four fractions:large macroaggregates(>2 mm),small macroaggregates(0.25–2 mm),microaggregates(0.053–0.25 mm),and silt/clay fractions(<0.053 mm).The results showed that organic amendments increased the organic C content and reduced the chemical,spectroscopic,thermogravimetric,and biological stability of organic C within soil aggregates relative to chemical fertilization alone.Within soil aggregates,the content of organic C was the highest in microaggregates and decreased in the order microaggregates>macroaggregates>silt/clay fractions.Meanwhile,organic C spectroscopic,thermogravimetric,and biological stability were the highest in silt/clay fractions,followed by macroaggregates and microaggregates.Moreover,the modified Walkley-Black method was not suitable for interpreting organic C stability at the aggregate scale due to the weak correlation between organic C chemical properties and other stability characteristics within the soil aggregates.These findings provide scientific insights at the aggregate scale into the changes of organic C properties under fertilization in greenhouse vegetable fields in China.
