The nano-TiO_2 particles were prepared by liquid hydrolysis method and characterized using XRD. Its antibacterial activity against two representative bacterial, Escherichia eoli and Staphylococcus aureus, was also stu...The nano-TiO_2 particles were prepared by liquid hydrolysis method and characterized using XRD. Its antibacterial activity against two representative bacterial, Escherichia eoli and Staphylococcus aureus, was also studied. The experimental results showed that the nano-TiO_2 caleinated at 600-700℃ contained the obvious anatase phase and exerted exeeUent antibacterial activity. The feature of antibacterial activity of nano- TiO_2 was non-strains specificity and exerted best antibacterial activity at concentration of 0.8 g/L.展开更多
A novel bioactive and bioresorbable PDLLA/chondroitin sulfate/chitosan scaffold was prepared via layer-by-layer（LBL） electrostatic-self-assembly （ESA） and the thermally induced phase separation （TIPS） technique....A novel bioactive and bioresorbable PDLLA/chondroitin sulfate/chitosan scaffold was prepared via layer-by-layer（LBL） electrostatic-self-assembly （ESA） and the thermally induced phase separation （TIPS） technique. Chondroitin sulfate and chitosan were alternately deposited on the activated PDLLA substrate. The deposition process was monitored by UV-Vis absorbance spectroscopy. After frozen and lyophilized, the scaffold was characterized by attenuated total reflection （ATR）-FT-IR, XPS, SEM and AFM. The results showed that the scaffold was modified uniformly with a dense inner layer with few detectable pores and a porous sponge outer layer with the pore size about 5 μm, there was an obvious across section and the average thickness of each layer was about 9.4 nm.展开更多
After co-cultrured osteoblast with fl-TCP ceramics, the cellular proliferating, mineralization and osteocalcin expression were studied. MTT assay showed that fl-TCP ceramics had no affect on cellular proliferating. La...After co-cultrured osteoblast with fl-TCP ceramics, the cellular proliferating, mineralization and osteocalcin expression were studied. MTT assay showed that fl-TCP ceramics had no affect on cellular proliferating. Laser scanning confocal detection showed that fl-TCP ceramics could increase the mineralization level of osteoblast. Furthermore, RT-PCR showed that fl-TCP could increase the expression level of osteocalcin. Those results indicate β-TCP ceramics had perfect biocompatibility and increased the mineralization of osteoblast to accelerate osteogenesis by means of affecting the expression of genes involving in osteogeneticprocess.展开更多
Stable and single-dispersed hydroxyapatite （HAP） nanoparticles were synthesized with ultrasonic-assisted method. HAP nanoparticles were characterized by dynamic light scattering, XRD （X-ray diffraction） and TEM （...Stable and single-dispersed hydroxyapatite （HAP） nanoparticles were synthesized with ultrasonic-assisted method. HAP nanoparticles were characterized by dynamic light scattering, XRD （X-ray diffraction） and TEM （Transmission Electron Microscopy）. The effect of HAP nanoparticles on the K562 human myelogenous leukemia cell line was investigated by MTT assay and cell count test, and the mechanism was studied through the changes of cell cycle and ultrastructure. The results showed that HAP nanoparticles inhibited the proliferation of K562 cells dramatically in vitro. HAP nanoparticles entered the cytoplasm of K562 cells and the cells were arrested at G/M phase, thus, the cells died directly.展开更多
To gain a better understanding of the anticancer effects of hydroxyapatite （HAP） nanoparticles in vivo and in vitro, the effects of the interaction of HAP nanoparticles with hepatoma cells were explored. HAP nanopar...To gain a better understanding of the anticancer effects of hydroxyapatite （HAP） nanoparticles in vivo and in vitro, the effects of the interaction of HAP nanoparticles with hepatoma cells were explored. HAP nanoparticles were prepared by homogeneous precipitation and characterized by laser particle analysis and transmission electron microscopy （TEM）. HAP nanoparticles were observed to be uniformly distributed, with rod-like shapes and diameters in the range of 42.1-87.1 nm. Overnight attached, suspended, and proliferating Bel-7402 cells were incubated with HAP nanoparticles. Inverted microscopy observation revealed that HAP nanoparticles with a cell membrane showed good adsorption. TEM demonstrated that HAP nanoparticles were present on the surface of cells, continuously taken up by cells through endocytosis, and transported in vesicles close to the nucleus. Fluorescence microscopy showed that the concentrations of intracellular Ca2＋ labeled with Fluo-3 calcium fluorescent probe were significantly enhanced. In addition, inverted microscopy observation revealed that suspended cells treated with HAP nanoparticles did not adhere to the culture bottle, resulting in cell death. After the overnight attached cells were treated with HAP nanoparticles for 96 h with increasing doses of HAP nanoparticles, inverted microscopy observation revealed that cell proliferation was slowed and ceU-ceU adhesion was weakened. Feulgen staining and image analysis indicated that the nuclear DNA content of the cells was markedly reduced, and argyrophilic nucleolar organizer region （AgNOR） staining and image analysis indicated that the number of AgNORs was significantly decreased. Therefore, hepatoma cells brought about the adsorption, uptake, transport and degradation of HAP nanoparticles. In addition, HAP nanoparticles affected hepatoma cells with regard to cell-cell adhesion, cell and extracellular matrix adhesion, and DNA and protein synthesis; thus inhibiting cell proliferation. This understanding of the effects of interaction between HAP nanoparticles and hepatoma cells is useful for further study of the anticancer mechanisms of HAP nanoparticles.展开更多
Schwann cells play a key role in peripheral nerve growth and regeneration. The aim of this study was to evaluate the effects of RGD peptides on Schwann cell behavior, and to identify the effects of the modified PDLLA ...Schwann cells play a key role in peripheral nerve growth and regeneration. The aim of this study was to evaluate the effects of RGD peptides on Schwann cell behavior, and to identify the effects of the modified PDLLA films with RGD in vivo. The results revealed that RGD coating with the concentration of 100-500 ug/mL promoted the cell proliferation and boosted the cell migration. Molecularly, RGD coating also enhanced the expression of the proliferation related genes （c-fos and c-jun） and the cell behavior related genes （actin, tublin, tau and MAP1） at first stages of the seeding, which is similar to the effects from laminin coating. In vivo, RGD addition improved the recovery efficiency of the transected nerve in regard of the more survived Schwann cells in vivo and the formation of more mature myelin sheath. Taken together, RGD peptides are good candidates to enhance the biocompatibility of the biomaterials and facilitate the peripheral nerve regeneration by prompting responses in Schwann ceils.展开更多
β-TCP ceramics drug carrier was first prepared and characterized. SEM showed that β-TCP carrier was in porous amorphous structure with diameters around 10 μm. The physical properties including apparent porosity, vo...β-TCP ceramics drug carrier was first prepared and characterized. SEM showed that β-TCP carrier was in porous amorphous structure with diameters around 10 μm. The physical properties including apparent porosity, volume-weight, tensile strength and the permeability were measured and the results indicated those properties fit the clinical usage of β-TCP drug carrier. Furthermore, drug release experiment in vitro showed that the carrier could prolong drug release in simulated body fluid which provides basis for the clinical use of β-TCP ceramics as drug carrier.展开更多
We prepared graphene（GE） with a mean size of 3087 nm.The transition of graphene oxide（GO） to GE was confirmed by UV-visible spectroscopy,Fourier transform infrared spectroscopy（FTIR） and X-ray diffraction（XRD）...We prepared graphene（GE） with a mean size of 3087 nm.The transition of graphene oxide（GO） to GE was confirmed by UV-visible spectroscopy,Fourier transform infrared spectroscopy（FTIR） and X-ray diffraction（XRD）.The experimental results of optical microscopic observation indicated that the GE ranged from 5 to 20 μg/mL did not affect the cell morphologies of the PC12 cells.The results of cell viability and membrane integrity assay supported that of optical microscopic observation and demonstrated that the GE ranged from 5 to 20 μg/mL presented no obvious cytotoxicity.However,reactive oxygen species（ROS） assay suggested that an elevation of ROS level could be detected when the GE ranged from 20 to 100 μg/mL.These results showed that the GE ranged from 5 to 10 μg/mL presented an excellent in vitro biocompatibility and was one kind of potential biomaterials for neural tissue engineering.展开更多
In this study, we synthesized degradable PRGD/PDLLA/β-TCP/NGF composites to facilitate neuronal repair. To this end, we (1) examined the release of nerve growth factor (NGF) from the composites, (2) evaluated the dif...In this study, we synthesized degradable PRGD/PDLLA/β-TCP/NGF composites to facilitate neuronal repair. To this end, we (1) examined the release of nerve growth factor (NGF) from the composites, (2) evaluated the differentiation status of the cells and (3) address how transcriptional activity may regulate the differentiation mechanism of these cells. NGF content was determined using enzyme-linked immunosorbent assay, while the cellular mRNA expression was examined by real-time PCR analysis. Our results indicated that NGF release was robust during the first 10 days and then stabilized at a lower level thereafter. Treatment of PC12 cells with the extract of the NGF-embedded composites induced the formation of neurites and, in some cases, net-like neurites. Analysis of the expression level of differentiation-related genes, such as TrkA, VGF, Rab1, GAP43 and β-tubulin Ⅱ, were significantly up-regulated. These findings suggest that these composites might be a suitable delivery system for growth factors like NGF that can be used to facilitate neuronal repair after injury.展开更多
基金Funded by the Research Fund of Key Laboratory for Advanced Technology in Environmental Protection of Jiangsu Province(No.AE201037the Foundation for Talent Recruitment of Yancheng Institute of Technology(No.XKR2011007)
文摘The nano-TiO_2 particles were prepared by liquid hydrolysis method and characterized using XRD. Its antibacterial activity against two representative bacterial, Escherichia eoli and Staphylococcus aureus, was also studied. The experimental results showed that the nano-TiO_2 caleinated at 600-700℃ contained the obvious anatase phase and exerted exeeUent antibacterial activity. The feature of antibacterial activity of nano- TiO_2 was non-strains specificity and exerted best antibacterial activity at concentration of 0.8 g/L.
基金the State Basic Research Foundation of China(No.2005CB623905)
文摘A novel bioactive and bioresorbable PDLLA/chondroitin sulfate/chitosan scaffold was prepared via layer-by-layer（LBL） electrostatic-self-assembly （ESA） and the thermally induced phase separation （TIPS） technique. Chondroitin sulfate and chitosan were alternately deposited on the activated PDLLA substrate. The deposition process was monitored by UV-Vis absorbance spectroscopy. After frozen and lyophilized, the scaffold was characterized by attenuated total reflection （ATR）-FT-IR, XPS, SEM and AFM. The results showed that the scaffold was modified uniformly with a dense inner layer with few detectable pores and a porous sponge outer layer with the pore size about 5 μm, there was an obvious across section and the average thickness of each layer was about 9.4 nm.
基金the Research Fund of Key Labortary for Advanced Technology in Environmental Protection of Jiangsu Province (No. AE201037)the Foundation for Talent Recruitment of Yancheng Institute of Technology (No. XKR2011007)the "973" Chinese National Key Fundamental Research and Development Program (No. G1999064701)
文摘After co-cultrured osteoblast with fl-TCP ceramics, the cellular proliferating, mineralization and osteocalcin expression were studied. MTT assay showed that fl-TCP ceramics had no affect on cellular proliferating. Laser scanning confocal detection showed that fl-TCP ceramics could increase the mineralization level of osteoblast. Furthermore, RT-PCR showed that fl-TCP could increase the expression level of osteocalcin. Those results indicate β-TCP ceramics had perfect biocompatibility and increased the mineralization of osteoblast to accelerate osteogenesis by means of affecting the expression of genes involving in osteogeneticprocess.
基金the Science and Technique Foundation of Hubei Province(No.2004AA303B03)
文摘Stable and single-dispersed hydroxyapatite （HAP） nanoparticles were synthesized with ultrasonic-assisted method. HAP nanoparticles were characterized by dynamic light scattering, XRD （X-ray diffraction） and TEM （Transmission Electron Microscopy）. The effect of HAP nanoparticles on the K562 human myelogenous leukemia cell line was investigated by MTT assay and cell count test, and the mechanism was studied through the changes of cell cycle and ultrastructure. The results showed that HAP nanoparticles inhibited the proliferation of K562 cells dramatically in vitro. HAP nanoparticles entered the cytoplasm of K562 cells and the cells were arrested at G/M phase, thus, the cells died directly.
基金Funded by the National Natural Science Foundation of China(Nos.81190133,51172171 and 51002109)the National Natural Science Foundation of Hubei Province((No.2013CFB354)the Excellent Youth Innovative Research Team Foundation and Talents Project of Hubei Polytechnic University(No.13xtz01)
文摘To gain a better understanding of the anticancer effects of hydroxyapatite （HAP） nanoparticles in vivo and in vitro, the effects of the interaction of HAP nanoparticles with hepatoma cells were explored. HAP nanoparticles were prepared by homogeneous precipitation and characterized by laser particle analysis and transmission electron microscopy （TEM）. HAP nanoparticles were observed to be uniformly distributed, with rod-like shapes and diameters in the range of 42.1-87.1 nm. Overnight attached, suspended, and proliferating Bel-7402 cells were incubated with HAP nanoparticles. Inverted microscopy observation revealed that HAP nanoparticles with a cell membrane showed good adsorption. TEM demonstrated that HAP nanoparticles were present on the surface of cells, continuously taken up by cells through endocytosis, and transported in vesicles close to the nucleus. Fluorescence microscopy showed that the concentrations of intracellular Ca2＋ labeled with Fluo-3 calcium fluorescent probe were significantly enhanced. In addition, inverted microscopy observation revealed that suspended cells treated with HAP nanoparticles did not adhere to the culture bottle, resulting in cell death. After the overnight attached cells were treated with HAP nanoparticles for 96 h with increasing doses of HAP nanoparticles, inverted microscopy observation revealed that cell proliferation was slowed and ceU-ceU adhesion was weakened. Feulgen staining and image analysis indicated that the nuclear DNA content of the cells was markedly reduced, and argyrophilic nucleolar organizer region （AgNOR） staining and image analysis indicated that the number of AgNORs was significantly decreased. Therefore, hepatoma cells brought about the adsorption, uptake, transport and degradation of HAP nanoparticles. In addition, HAP nanoparticles affected hepatoma cells with regard to cell-cell adhesion, cell and extracellular matrix adhesion, and DNA and protein synthesis; thus inhibiting cell proliferation. This understanding of the effects of interaction between HAP nanoparticles and hepatoma cells is useful for further study of the anticancer mechanisms of HAP nanoparticles.
文摘Schwann cells play a key role in peripheral nerve growth and regeneration. The aim of this study was to evaluate the effects of RGD peptides on Schwann cell behavior, and to identify the effects of the modified PDLLA films with RGD in vivo. The results revealed that RGD coating with the concentration of 100-500 ug/mL promoted the cell proliferation and boosted the cell migration. Molecularly, RGD coating also enhanced the expression of the proliferation related genes （c-fos and c-jun） and the cell behavior related genes （actin, tublin, tau and MAP1） at first stages of the seeding, which is similar to the effects from laminin coating. In vivo, RGD addition improved the recovery efficiency of the transected nerve in regard of the more survived Schwann cells in vivo and the formation of more mature myelin sheath. Taken together, RGD peptides are good candidates to enhance the biocompatibility of the biomaterials and facilitate the peripheral nerve regeneration by prompting responses in Schwann ceils.
基金Funded by the "973" Chinese National Key Fundamental Research and Development Program (No.G1999064701)the Research Fund of Key Laboratory for Advanced Technology in Environmental Protection of Jiangsu Province (AE201037)
文摘β-TCP ceramics drug carrier was first prepared and characterized. SEM showed that β-TCP carrier was in porous amorphous structure with diameters around 10 μm. The physical properties including apparent porosity, volume-weight, tensile strength and the permeability were measured and the results indicated those properties fit the clinical usage of β-TCP drug carrier. Furthermore, drug release experiment in vitro showed that the carrier could prolong drug release in simulated body fluid which provides basis for the clinical use of β-TCP ceramics as drug carrier.
基金Funded by the Natural Science Foundation of Hubei Province(No.2014CFB839)the Doctoral Research Fund of Wuhan University of Technology(No.471-40120093)+4 种基金the Opening Project of Jiangsu Provincial Key Laboratory of Silk Engineering(No.KJS1415)the Hong Kong,Macao and Taiwan Science&Technology Cooperation Program of China(No.2015DFH30180)the Fundamental Research Funds for the Central Universities(No.WUT:2014-Ⅶ-028)the National Natural Science Foundation of China(No.51403168)the Key Project of Science and Technology of Wuhan(No.2014060202010120)
文摘We prepared graphene（GE） with a mean size of 3087 nm.The transition of graphene oxide（GO） to GE was confirmed by UV-visible spectroscopy,Fourier transform infrared spectroscopy（FTIR） and X-ray diffraction（XRD）.The experimental results of optical microscopic observation indicated that the GE ranged from 5 to 20 μg/mL did not affect the cell morphologies of the PC12 cells.The results of cell viability and membrane integrity assay supported that of optical microscopic observation and demonstrated that the GE ranged from 5 to 20 μg/mL presented no obvious cytotoxicity.However,reactive oxygen species（ROS） assay suggested that an elevation of ROS level could be detected when the GE ranged from 20 to 100 μg/mL.These results showed that the GE ranged from 5 to 10 μg/mL presented an excellent in vitro biocompatibility and was one kind of potential biomaterials for neural tissue engineering.
基金supported by the National Basic Research Program of China (2011CB606205)
文摘In this study, we synthesized degradable PRGD/PDLLA/β-TCP/NGF composites to facilitate neuronal repair. To this end, we (1) examined the release of nerve growth factor (NGF) from the composites, (2) evaluated the differentiation status of the cells and (3) address how transcriptional activity may regulate the differentiation mechanism of these cells. NGF content was determined using enzyme-linked immunosorbent assay, while the cellular mRNA expression was examined by real-time PCR analysis. Our results indicated that NGF release was robust during the first 10 days and then stabilized at a lower level thereafter. Treatment of PC12 cells with the extract of the NGF-embedded composites induced the formation of neurites and, in some cases, net-like neurites. Analysis of the expression level of differentiation-related genes, such as TrkA, VGF, Rab1, GAP43 and β-tubulin Ⅱ, were significantly up-regulated. These findings suggest that these composites might be a suitable delivery system for growth factors like NGF that can be used to facilitate neuronal repair after injury.
