Nostoc flagelliforme is a terrestrial cyanobacterium that can resist many types of stressors,including drought,ultraviolet radiation,and extreme temperatures.In this study,we identified the drought tolerance gene Nfcr...Nostoc flagelliforme is a terrestrial cyanobacterium that can resist many types of stressors,including drought,ultraviolet radiation,and extreme temperatures.In this study,we identified the drought tolerance gene NfcrtO,which encodes aβ-carotene ketolase,through screening the transcriptome of N.flagelliforme under water loss stress.Prokaryotic expression of NfcrtO under 0.6 mol/L sorbitol or under 0.3 mol/L NaCl stress significantly increased the growth rate of Escherichia coli.When NfcrtO was heterologously expressed in rice,the seedling height and root length of NfcrtO-overexpressing rice plants were significantly higher than those of the wild type(WT)plants grown on½Murashige and Skoog solid medium with 120 mmol/L mannitol at the seedling stage.Transcriptome analysis revealed that NfcrtO was involved in osmotic stress,antioxidant,and other stress-related pathways.Additionally,the survival rate of the NfcrtO-overexpression lines was significantly higher than that of the WT line under both hydroponic stress(24%PEG and 100 mmol/L H_(2)O_(2))and soil drought treatment at the seedling stage.Physiological traits,including the activity levels of superoxide dismutase,peroxidase,catalase,total antioxidant capacity,and the contents of proline,trehalose,and soluble sugar,were significantly improved in the NfcrtO-overexpression lines relative to those in the WT line under 20%PEG treatment.Furthermore,when water was withheld at the booting stage,the grain yield per plant of NfcrtO-overexpression lines was significantly higher than that of the WT line.Yeast two-hybrid analysis identified interactions between NfcrtO and Dna J protein,E3 ubiquitin-protein ligase,and pyrophosphate-energized vacuolar membrane proton pump.Thus,heterologous expression of NfcrtO in rice could significantly improve the tolerance of rice to osmotic stress,potentially facilitating the development of new rice varieties.展开更多
Urban construction in China has changed from“extensional sprawl”to“connotative development”.With the accelerating urbanization and improvement of living standards,citizens have had higher and more requirements on ...Urban construction in China has changed from“extensional sprawl”to“connotative development”.With the accelerating urbanization and improvement of living standards,citizens have had higher and more requirements on urban waterfront spaces.Starting from the history and characteristics of comprehensive river regulation,this paper explored its multiple meanings against the new appeals of urban development.On this basis,design and planning approaches were discussed from 3 perspectives of waterfront space planning control,waterfront space design optimization,and improvement of waterfront landscape,then the planning and design strategies of urban waterfront spaces were explored by studying the comprehensive waterfront space regulation project of the Old Huan River in Hubei,so as to provide references for the comprehensive water system regulation and space planning and construction of urban waterfront spaces.展开更多
Comprehensive river regulation in the new era is a series of construction procedures from planning,scheme generation and engineering design to commercial pattern,construction and operation,etc.,which is a comprehensiv...Comprehensive river regulation in the new era is a series of construction procedures from planning,scheme generation and engineering design to commercial pattern,construction and operation,etc.,which is a comprehensive project coordinated and managed by multiple departments.Different industry departments measure the feasibility of scheme design according to the standards of their own departments in the examination of river regulation,but due to the lack of understanding of other industry standards,some unnecessary scheme contradictions are often caused in the cross-industry cooperation.The study integrates and sorts out specifi cations of various departments involved in river regulation,and performs analogy study of standards of various contents in multi-industry cooperation with contents of comprehensive river regulation as the scale,so as to promote cross-industry communication of comprehensive river regulation and promote construction of integrated river regulation platform.展开更多
ERF (Ethylene-responsive factor)转录因子家族是AP2-EREBP (APETALA2 and ethylene-responsive element binding proteins, AP2/ERF)超家族中的一个具有较多成员的家族,其在植物中广泛存在并在植物生长发育过程中发挥重要作用。在水稻...ERF (Ethylene-responsive factor)转录因子家族是AP2-EREBP (APETALA2 and ethylene-responsive element binding proteins, AP2/ERF)超家族中的一个具有较多成员的家族,其在植物中广泛存在并在植物生长发育过程中发挥重要作用。在水稻中ERF转录因子家族成员的OsERF74为水稻干旱响应模块7的节点基因。本研究从水稻品种‘日本晴’中克隆了OsERF74,通过生物信息学和蛋白质系统发育树分析显示OsERF74为ERF类转录因子,具有高度保守的AP2结构域,并在其N端具有跨膜结构域;在烟草中对OsERF74进行亚细胞定位显示其定位于细胞膜上;OsERF74在水稻营养生长和生殖生长时期的各个组织中均有表达,而在苗期的根和叶组织中OsERF74的表达受到多种激素和非生物胁迫的调节;为了解OsERF74基因功能,构建并获得了超表达转基因植株。通过高渗透胁迫下的种子萌发实验显示OsERF74超表达能够增强水稻种子在高渗透胁迫下的萌发率。本研究初步探究了OsERF74的特性与功能,为进一步挖掘水稻干旱响应功能基因提供基础。展开更多
基金supported by the National Key Research and Development Program of China(Grant No.2018YFE0106200)the Science and Technology Research Project of Jiangxi Provincial Department of Education,China(Grant No.K4100131)the Science and Technology Research Project of Shangrao,Jiangxi Province,China(Grant No.K4000019).
文摘Nostoc flagelliforme is a terrestrial cyanobacterium that can resist many types of stressors,including drought,ultraviolet radiation,and extreme temperatures.In this study,we identified the drought tolerance gene NfcrtO,which encodes aβ-carotene ketolase,through screening the transcriptome of N.flagelliforme under water loss stress.Prokaryotic expression of NfcrtO under 0.6 mol/L sorbitol or under 0.3 mol/L NaCl stress significantly increased the growth rate of Escherichia coli.When NfcrtO was heterologously expressed in rice,the seedling height and root length of NfcrtO-overexpressing rice plants were significantly higher than those of the wild type(WT)plants grown on½Murashige and Skoog solid medium with 120 mmol/L mannitol at the seedling stage.Transcriptome analysis revealed that NfcrtO was involved in osmotic stress,antioxidant,and other stress-related pathways.Additionally,the survival rate of the NfcrtO-overexpression lines was significantly higher than that of the WT line under both hydroponic stress(24%PEG and 100 mmol/L H_(2)O_(2))and soil drought treatment at the seedling stage.Physiological traits,including the activity levels of superoxide dismutase,peroxidase,catalase,total antioxidant capacity,and the contents of proline,trehalose,and soluble sugar,were significantly improved in the NfcrtO-overexpression lines relative to those in the WT line under 20%PEG treatment.Furthermore,when water was withheld at the booting stage,the grain yield per plant of NfcrtO-overexpression lines was significantly higher than that of the WT line.Yeast two-hybrid analysis identified interactions between NfcrtO and Dna J protein,E3 ubiquitin-protein ligase,and pyrophosphate-energized vacuolar membrane proton pump.Thus,heterologous expression of NfcrtO in rice could significantly improve the tolerance of rice to osmotic stress,potentially facilitating the development of new rice varieties.
基金Cross Exploration Project Funded by Basic Research Expenses of the Central Universities(DUT19JC55)Dalian Innovation Support Program for High-end Talents(2017RQ076).
文摘Urban construction in China has changed from“extensional sprawl”to“connotative development”.With the accelerating urbanization and improvement of living standards,citizens have had higher and more requirements on urban waterfront spaces.Starting from the history and characteristics of comprehensive river regulation,this paper explored its multiple meanings against the new appeals of urban development.On this basis,design and planning approaches were discussed from 3 perspectives of waterfront space planning control,waterfront space design optimization,and improvement of waterfront landscape,then the planning and design strategies of urban waterfront spaces were explored by studying the comprehensive waterfront space regulation project of the Old Huan River in Hubei,so as to provide references for the comprehensive water system regulation and space planning and construction of urban waterfront spaces.
基金Interdisciplinary Research Projects of the Fundamental Research Funds for the Central Universities(DUT19JC55)High-level Talents Innovation Support Project of Dalian City(2017RQ076).
文摘Comprehensive river regulation in the new era is a series of construction procedures from planning,scheme generation and engineering design to commercial pattern,construction and operation,etc.,which is a comprehensive project coordinated and managed by multiple departments.Different industry departments measure the feasibility of scheme design according to the standards of their own departments in the examination of river regulation,but due to the lack of understanding of other industry standards,some unnecessary scheme contradictions are often caused in the cross-industry cooperation.The study integrates and sorts out specifi cations of various departments involved in river regulation,and performs analogy study of standards of various contents in multi-industry cooperation with contents of comprehensive river regulation as the scale,so as to promote cross-industry communication of comprehensive river regulation and promote construction of integrated river regulation platform.
文摘ERF (Ethylene-responsive factor)转录因子家族是AP2-EREBP (APETALA2 and ethylene-responsive element binding proteins, AP2/ERF)超家族中的一个具有较多成员的家族,其在植物中广泛存在并在植物生长发育过程中发挥重要作用。在水稻中ERF转录因子家族成员的OsERF74为水稻干旱响应模块7的节点基因。本研究从水稻品种‘日本晴’中克隆了OsERF74,通过生物信息学和蛋白质系统发育树分析显示OsERF74为ERF类转录因子,具有高度保守的AP2结构域,并在其N端具有跨膜结构域;在烟草中对OsERF74进行亚细胞定位显示其定位于细胞膜上;OsERF74在水稻营养生长和生殖生长时期的各个组织中均有表达,而在苗期的根和叶组织中OsERF74的表达受到多种激素和非生物胁迫的调节;为了解OsERF74基因功能,构建并获得了超表达转基因植株。通过高渗透胁迫下的种子萌发实验显示OsERF74超表达能够增强水稻种子在高渗透胁迫下的萌发率。本研究初步探究了OsERF74的特性与功能,为进一步挖掘水稻干旱响应功能基因提供基础。
