Long non-coding RNAs(lncRNAs)are emerging as powerful regulators of adipocyte differentiation,fat metabolism and gene expression.However,the functional roles and mechanisms of lncRNAs in these processes remain unclear...Long non-coding RNAs(lncRNAs)are emerging as powerful regulators of adipocyte differentiation,fat metabolism and gene expression.However,the functional roles and mechanisms of lncRNAs in these processes remain unclear.Here,we identified a novel antisense transcript,named APMAP-AS,transcribed from adipocyte membrane-associated protein(APMAP)in the pig genome.APMAP-AS and APMAP were highly expressed in retroperitoneal adipose of obese pigs,compared with that in control pigs.Using a bone mesenchymal stem cells(BMSCs)adipogenic differentiation model,we found that APMAP-AS positively regulated adipogenic differentiation.APMAP-AS had the potential to form an RNA–RNA duplex with APMAP,and increased the stability of APMAP mRNA.Additionally,APMAP-AS promoted lipid metabolism and inhibited the expression of inflammatory factors.These findings of a natural antisense transcript for a regulatory gene associated with lipid synthesis might further our understanding of lncRNAs in driving adaptive adipose tissue remodeling and preserving metabolic health.展开更多
基金supported by grants from the National Key R&D Program of China(2021YFA0805903,2020YFA0509500 and 2021YFD1300800)the Sichuan Science and Technology Program,China(2021ZDZX0008,2021YFYZ0009,2021YFYZ0030 and 2022JDJQ0054).
文摘Long non-coding RNAs(lncRNAs)are emerging as powerful regulators of adipocyte differentiation,fat metabolism and gene expression.However,the functional roles and mechanisms of lncRNAs in these processes remain unclear.Here,we identified a novel antisense transcript,named APMAP-AS,transcribed from adipocyte membrane-associated protein(APMAP)in the pig genome.APMAP-AS and APMAP were highly expressed in retroperitoneal adipose of obese pigs,compared with that in control pigs.Using a bone mesenchymal stem cells(BMSCs)adipogenic differentiation model,we found that APMAP-AS positively regulated adipogenic differentiation.APMAP-AS had the potential to form an RNA–RNA duplex with APMAP,and increased the stability of APMAP mRNA.Additionally,APMAP-AS promoted lipid metabolism and inhibited the expression of inflammatory factors.These findings of a natural antisense transcript for a regulatory gene associated with lipid synthesis might further our understanding of lncRNAs in driving adaptive adipose tissue remodeling and preserving metabolic health.
