Background:This study aimed to construct and characterize a humanized influenza mouse model expressing hST6GAL1.Methods:Humanized fragments,consisting of the endothelial cell-specific K18 promoter,human ST6GAL1-encodi...Background:This study aimed to construct and characterize a humanized influenza mouse model expressing hST6GAL1.Methods:Humanized fragments,consisting of the endothelial cell-specific K18 promoter,human ST6GAL1-encoding gene,and luciferase gene,were microinjected into the fertilized eggs of mice.The manipulated embryos were transferred into the oviducts of pseudopregnant female mice.The offspring were identified using PCR.Mice exhibiting elevated expression of the hST6GAL1 gene were selectively bred for propagation,and in vivo analysis was performed for screening.Expression of the humanized gene was tested by performing immunohistochemical(IHC)analysis.Hematologic and biochemical analyses using the whole blood and serum of humanized hST6GAL1 mice were performed.Results:Successful integration of the human ST6GAL1 gene into the mouse genome led to the overexpression of human SiaT ST6GAL1.Seven mice were identified as carrying copies of the humanized gene,and the in vivo analysis indicated that hST6GAL1gene expression in positive mice mirrored influenza virus infection characteristics.The IHC results revealed that hST6GAL1 was expressed in the lungs of humanized mice.Moreover,the hematologic and biochemical parameters of the positive mice were within the normal range.Conclusion:A humanized influenza mouse model expressing the hST6GAL1 gene was successfully established and characterized.展开更多
The green innovation value chain is a key step in transforming green,innovative scientific,and technological achievements into productive forces.The establishment of green innovation value chains based on value distri...The green innovation value chain is a key step in transforming green,innovative scientific,and technological achievements into productive forces.The establishment of green innovation value chains based on value distribution rather than technical conditions can effectively overcome the common bottleneck faced by different nations during their green innovation endeavors,namely,the substitution of conventional products with green alternatives.This study investigates the impeded diffusion of green products and their underlying causes,analyzes the internal structure and mechanism of the green innovation value chain,and explores the establishment of regional green innovation value chains and the models available for value chain upgrading.展开更多
Cultivated land is an important natural resource to ensure food,ecological and economic security.The cultivated land quality evaluation(CQE)is greatly significant for protecting and managing cultivated land.In this st...Cultivated land is an important natural resource to ensure food,ecological and economic security.The cultivated land quality evaluation(CQE)is greatly significant for protecting and managing cultivated land.In this study,320 counties in the black soil region of Northeast China(BSRNC)represent the research units used to construct the CQE system measuring the soil properties(SP),cultivated land productivity(CLP),ecological environment(EE)and social economy(SE).The total of 19 factors were selected to calculate the integrated fertility index(IFI)and divided into grades.Simultaneously,we used the coupling coordination degree model to comprehensively analyze the spatial pattern of the cultivated land quality(CLQ)in the BSRNC,and use the structural equation model(SEM)to analyze the driving mechanism.The results show the following:1)The CLQ of 262 counties in the BSRNC is in a state of coupling and coordination,and the coupling and coordination degree presents a spatial distribution pattern of‘high in the southwest and northeast,low in the northwest and southeast’.The coordinated development degree of 271 counties is between 0.4 and 0.6,which is in a transitional state between coordination and disorder.2)The CLQ in the BSRNC is generally good,with an average grade of 3.High-quality cultivated land accounts for 58.45%of all counties,middle-and upper-quality cultivated land accounts for 27.05%,and poor-quality cultivated land accounts for 14.49%.3)The SEM analysis shows that the SP,CLP,EE,and SE all influence the CLQ.Among them,the SP has the largest driving force on the CLQ,while the SE has the smallest driving force on it.The results confirm that the main factors affecting the evaluation results are crop productivity level,normalized difference vegetation index,ratio vegetation index,difference vegetation index,and organic carbon content.When implementing protection measures in counties with a low CLQ,considering a balanced coordination of multiple systems and reasonably controlling the quality degradation are important.This study provides the current situation and driving factors of the CLQ in the BSRNC and will play an important role in black soil governance and utilization.展开更多
目的探讨β胶原特殊序列(β-crosslaps,β-CTX)、总1型胶原氨基端延长肽(t-P1NP)及N端中段骨钙素(N-MID-OT)在绝经后女性骨质疏松性骨折风险评估中的应用价值。方法回顾性分析2020年2月至2022年12月解放军总医院第六医学中心收治的102...目的探讨β胶原特殊序列(β-crosslaps,β-CTX)、总1型胶原氨基端延长肽(t-P1NP)及N端中段骨钙素(N-MID-OT)在绝经后女性骨质疏松性骨折风险评估中的应用价值。方法回顾性分析2020年2月至2022年12月解放军总医院第六医学中心收治的102例绝经后骨质疏松(postmenopausal osteoporosis,PMOP)患者的临床资料,将其作为研究组,并根据是否发生骨折将其分为PMOP骨折组(39例)与PMOP未骨折组(63例),另选100名健康体检者作为对照组。记录所有研究对象的β-CTX、t-P1NP及N-MID-OT水平并进行比较,用ROC曲线评价β-CTX、t-P1NP及N-MID-OT在绝经后女性骨质疏松性骨折风险评估中的应用价值。结果研究组的平均体重、体质量指数、股骨颈骨密度(bone mineral density,BMD)、左髋总和BMD及L 1~4总和BMD均明显低于对照组(P<0.05);而两组研究对象的平均年龄、平均身高及绝经年龄等比较差异均无统计学意义(P>0.05)。研究组的β-CTX、t-P1NP及N-MID-OT均明显高于对照组(t值依次为12.688、37.430、26.599,P<0.05)。β-CTX+t-P1NP+N-MID-OT联合检测用于预测PMOP的AUC值为0.978,敏感度为98.04%,特异度为97.00%,表明β-CTX+t-P1NP+N-MID-OT三指标联合检测用于预测PMOP的效能更高。PMOP骨折组的β-CTX、t-P1NP及N-MID-OT均明显高于PMOP未骨折组(t值依次为6.078、16.363、12.227,P<0.05)。β-CTX+t-P1NP+N-MID-OT联合检测用于评估PMOP骨折风险的AUC值为0.939,敏感度为94.87%,特异度为95.24%,表明β-CTX+t-P1NP+N-MID-OT联合检测用于评估PMOP骨折风险的效能更高。结论PMOP患者β-CTX、t-P1NP及N-MID-OT水平均明显升高,而PMOP骨折患者β-CTX、t-P1NP及N-MID-OT水平升高更为明显,且β-CTX+t-P1NP+N-MID-OT联合检测可显著提高对PMOP预测及骨折风险评估效能,值得借鉴。展开更多
基金National Key Research and Development Program of China,Grant/Award Number:2021YFC2301403 and 2022YFF0711000。
文摘Background:This study aimed to construct and characterize a humanized influenza mouse model expressing hST6GAL1.Methods:Humanized fragments,consisting of the endothelial cell-specific K18 promoter,human ST6GAL1-encoding gene,and luciferase gene,were microinjected into the fertilized eggs of mice.The manipulated embryos were transferred into the oviducts of pseudopregnant female mice.The offspring were identified using PCR.Mice exhibiting elevated expression of the hST6GAL1 gene were selectively bred for propagation,and in vivo analysis was performed for screening.Expression of the humanized gene was tested by performing immunohistochemical(IHC)analysis.Hematologic and biochemical analyses using the whole blood and serum of humanized hST6GAL1 mice were performed.Results:Successful integration of the human ST6GAL1 gene into the mouse genome led to the overexpression of human SiaT ST6GAL1.Seven mice were identified as carrying copies of the humanized gene,and the in vivo analysis indicated that hST6GAL1gene expression in positive mice mirrored influenza virus infection characteristics.The IHC results revealed that hST6GAL1 was expressed in the lungs of humanized mice.Moreover,the hematologic and biochemical parameters of the positive mice were within the normal range.Conclusion:A humanized influenza mouse model expressing the hST6GAL1 gene was successfully established and characterized.
基金the part of the“Research on Paths to High-quality Development in Agriculture Against the Backdrop of Rural Revitalization,”a project of the Publicity Department of the CPC Central Committee for Young Talents in Publicity,Socialist Thought and Cultural Promotionthe National Social Science Fund of China-supported project,“Research on Theoretical Logic and Realization Path of Urban-Rural Integration Based on Industrial Internet”(21XJL001)+1 种基金the major project of Sichuan province in philosophy and social science planning,“Research on the Innovation and Policy Adaptation of Sichuan’s Agricultural Green Development System under the‘Dual Carbon’Goal”(SC22ZDYC44)the key project of Sichuan province in soft science research and planning,“Research on the Path to Peak Carbon and Carbon Neutrality of the Agricultural Sector in Rural Areas of Sichuan Province”(2022JDR0157).
文摘The green innovation value chain is a key step in transforming green,innovative scientific,and technological achievements into productive forces.The establishment of green innovation value chains based on value distribution rather than technical conditions can effectively overcome the common bottleneck faced by different nations during their green innovation endeavors,namely,the substitution of conventional products with green alternatives.This study investigates the impeded diffusion of green products and their underlying causes,analyzes the internal structure and mechanism of the green innovation value chain,and explores the establishment of regional green innovation value chains and the models available for value chain upgrading.
基金Under the auspices of National Key R&D Program of China(No.2021YFD1500104-4)National Natural Science Foundation of China(No.42171407,42077242)+1 种基金Natural Science Foundation of Jilin Province(No.20210101098JC)Special Investigation on Basic Science and Technology Resources(No.2021FY100406)。
文摘Cultivated land is an important natural resource to ensure food,ecological and economic security.The cultivated land quality evaluation(CQE)is greatly significant for protecting and managing cultivated land.In this study,320 counties in the black soil region of Northeast China(BSRNC)represent the research units used to construct the CQE system measuring the soil properties(SP),cultivated land productivity(CLP),ecological environment(EE)and social economy(SE).The total of 19 factors were selected to calculate the integrated fertility index(IFI)and divided into grades.Simultaneously,we used the coupling coordination degree model to comprehensively analyze the spatial pattern of the cultivated land quality(CLQ)in the BSRNC,and use the structural equation model(SEM)to analyze the driving mechanism.The results show the following:1)The CLQ of 262 counties in the BSRNC is in a state of coupling and coordination,and the coupling and coordination degree presents a spatial distribution pattern of‘high in the southwest and northeast,low in the northwest and southeast’.The coordinated development degree of 271 counties is between 0.4 and 0.6,which is in a transitional state between coordination and disorder.2)The CLQ in the BSRNC is generally good,with an average grade of 3.High-quality cultivated land accounts for 58.45%of all counties,middle-and upper-quality cultivated land accounts for 27.05%,and poor-quality cultivated land accounts for 14.49%.3)The SEM analysis shows that the SP,CLP,EE,and SE all influence the CLQ.Among them,the SP has the largest driving force on the CLQ,while the SE has the smallest driving force on it.The results confirm that the main factors affecting the evaluation results are crop productivity level,normalized difference vegetation index,ratio vegetation index,difference vegetation index,and organic carbon content.When implementing protection measures in counties with a low CLQ,considering a balanced coordination of multiple systems and reasonably controlling the quality degradation are important.This study provides the current situation and driving factors of the CLQ in the BSRNC and will play an important role in black soil governance and utilization.
文摘目的探讨β胶原特殊序列(β-crosslaps,β-CTX)、总1型胶原氨基端延长肽(t-P1NP)及N端中段骨钙素(N-MID-OT)在绝经后女性骨质疏松性骨折风险评估中的应用价值。方法回顾性分析2020年2月至2022年12月解放军总医院第六医学中心收治的102例绝经后骨质疏松(postmenopausal osteoporosis,PMOP)患者的临床资料,将其作为研究组,并根据是否发生骨折将其分为PMOP骨折组(39例)与PMOP未骨折组(63例),另选100名健康体检者作为对照组。记录所有研究对象的β-CTX、t-P1NP及N-MID-OT水平并进行比较,用ROC曲线评价β-CTX、t-P1NP及N-MID-OT在绝经后女性骨质疏松性骨折风险评估中的应用价值。结果研究组的平均体重、体质量指数、股骨颈骨密度(bone mineral density,BMD)、左髋总和BMD及L 1~4总和BMD均明显低于对照组(P<0.05);而两组研究对象的平均年龄、平均身高及绝经年龄等比较差异均无统计学意义(P>0.05)。研究组的β-CTX、t-P1NP及N-MID-OT均明显高于对照组(t值依次为12.688、37.430、26.599,P<0.05)。β-CTX+t-P1NP+N-MID-OT联合检测用于预测PMOP的AUC值为0.978,敏感度为98.04%,特异度为97.00%,表明β-CTX+t-P1NP+N-MID-OT三指标联合检测用于预测PMOP的效能更高。PMOP骨折组的β-CTX、t-P1NP及N-MID-OT均明显高于PMOP未骨折组(t值依次为6.078、16.363、12.227,P<0.05)。β-CTX+t-P1NP+N-MID-OT联合检测用于评估PMOP骨折风险的AUC值为0.939,敏感度为94.87%,特异度为95.24%,表明β-CTX+t-P1NP+N-MID-OT联合检测用于评估PMOP骨折风险的效能更高。结论PMOP患者β-CTX、t-P1NP及N-MID-OT水平均明显升高,而PMOP骨折患者β-CTX、t-P1NP及N-MID-OT水平升高更为明显,且β-CTX+t-P1NP+N-MID-OT联合检测可显著提高对PMOP预测及骨折风险评估效能,值得借鉴。
