The second internal transcribed spacer (ITS-2) of Leucocytozoon caulleryi was amplified by PCR using a pair of conserved primers and cloned into the T-T windows of plasmid pGEM-T easy vector. The inserts were successf...The second internal transcribed spacer (ITS-2) of Leucocytozoon caulleryi was amplified by PCR using a pair of conserved primers and cloned into the T-T windows of plasmid pGEM-T easy vector. The inserts were successfully sequenced and the results revealed that the ITS-2 plus flanking sequence (ITS2+) was composed of 270 nucleotides. and the ITS-2 was 113 bp in length. The ITS-2 of L. caulleryi was analysed by NCBI Blast, and the degree of homology of the ITS-2 of L. caulleryi was compared with that of Eimeria tenella , Candida tropicalis and Saccharomyces kluyveri and others by wDNASIS. The results showed that the ITS-2 of L. caulleryi is characteristic and has the greatest similarity with E. tenella (21.2%).展开更多
In this paper, the erosion-resisting coefficient was introduced to computebed deformation in a crush bedrock river. In the case of crush bedrock, there has been no propercontrol equation to describe bed stability, whi...In this paper, the erosion-resisting coefficient was introduced to computebed deformation in a crush bedrock river. In the case of crush bedrock, there has been no propercontrol equation to describe bed stability, which leads to difficulty in calculation of the beddeformation with conventional methods. The data from field survey were used to give thee-rosion-resisting capability with an appropriate coefficient. After the determination oflongitudinal distribution expressed by polynomial regression and transversal distribution expressedby normal distribution function, the plane distribution of erosion-resisting coefficient in a crushbedrock river was obtained. With the computational results from a 2-D horizontal flow mathematicalmodel, the erosion-resisting coefficient and controlling condition of local stability were employedto compute the values of bed deformation when riverbed is stable. The above method was applied in acase study, and the computational results of flow and bed deformations are in good a-greement withphysical model test data.展开更多
基金supported by grants from National Natural Science Foundation of China(39870549)Natural Science Foundation of Guangdong Province(980134).
文摘The second internal transcribed spacer (ITS-2) of Leucocytozoon caulleryi was amplified by PCR using a pair of conserved primers and cloned into the T-T windows of plasmid pGEM-T easy vector. The inserts were successfully sequenced and the results revealed that the ITS-2 plus flanking sequence (ITS2+) was composed of 270 nucleotides. and the ITS-2 was 113 bp in length. The ITS-2 of L. caulleryi was analysed by NCBI Blast, and the degree of homology of the ITS-2 of L. caulleryi was compared with that of Eimeria tenella , Candida tropicalis and Saccharomyces kluyveri and others by wDNASIS. The results showed that the ITS-2 of L. caulleryi is characteristic and has the greatest similarity with E. tenella (21.2%).
文摘In this paper, the erosion-resisting coefficient was introduced to computebed deformation in a crush bedrock river. In the case of crush bedrock, there has been no propercontrol equation to describe bed stability, which leads to difficulty in calculation of the beddeformation with conventional methods. The data from field survey were used to give thee-rosion-resisting capability with an appropriate coefficient. After the determination oflongitudinal distribution expressed by polynomial regression and transversal distribution expressedby normal distribution function, the plane distribution of erosion-resisting coefficient in a crushbedrock river was obtained. With the computational results from a 2-D horizontal flow mathematicalmodel, the erosion-resisting coefficient and controlling condition of local stability were employedto compute the values of bed deformation when riverbed is stable. The above method was applied in acase study, and the computational results of flow and bed deformations are in good a-greement withphysical model test data.
