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Metformin accelerates bone fracture healing by promoting type H vessel formation through inhibition of YAP1/TAZ expression 被引量:4
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作者 Zhe Ruan Hao Yin +26 位作者 Teng-Fei Wan Zhi-Rou Lin Shu-Shan Zhao Hai-Tao Long Cheng Long Zhao-Hui Li Yu-Qi Liu Hao Luo Liang Cheng Can Chen Min Zeng Zhang-Yuan Lin Rui-Bo Zhao Chun-Yuan Chen Zhen-Xing Wang Zheng-Zhao Liu Jia Cao Yi-Yi Wang ling jin Yi-Wei Liu Guo-Qiang Zhu jing-Tao Zou Jiang-Shan Gong Yi Luo Yin Hu Yong Zhu Hui Xie 《Bone Research》 SCIE CAS CSCD 2023年第3期625-637,共13页
Due to increasing morbidity worldwide,fractures are becoming an emerging public health concern.This study aimed to investigate the effect of metformin on the healing of osteoporotic as well as normal fractures.Type H ... Due to increasing morbidity worldwide,fractures are becoming an emerging public health concern.This study aimed to investigate the effect of metformin on the healing of osteoporotic as well as normal fractures.Type H vessels have recently been identified as a bone-specific vascular subtype that supports osteogenesis.Here,we show that metformin accelerated fracture healing in both osteoporotic and normal mice.Moreover,metformin promoted angiogenesis in vitro under hypoxia as well as type H vessel formation throughout fracture healing.Mechanistically,metformin increased the expression of HIF-1α,an important positive regulator of type H vessel formation,by inhibiting the expression of YAP1/TAZ in calluses and hypoxia-cultured human microvascular endothelial cells(HMECs).The results of HIF-1αor YAP1/TAZ interference in hypoxia-cultured HMECs using si RNA further suggested that the enhancement of HIF-1αand its target genes by metformin is primarily through YAP1/TAZ inhibition.Finally,overexpression of YAP1/TAZ partially counteracted the effect of metformin in promoting type H vessel-induced angiogenesis-osteogenesis coupling during fracture repair.In summary,our findings suggest that metformin has the potential to be a therapeutic agent for fractures by promoting type H vessel formation through YAP1/TAZ inhibition. 展开更多
关键词 YAP1 HEALING FRACTURE
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预测儿童Ⅱ期人工晶状体植入术后青光眼相关不良事件的风险:一项为期3年的研究 被引量:1
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作者 Hui Chen Chaoqun Xu +13 位作者 ling jin Zhenyu Wang jingmin Xu Yingshi Zou Guangming jin Lixia Luo Haotian Lin Weirong Chen Danying Zheng Yizhi Liu Zhenzhen Liu 金佳昕(审校) 陈子东(审校) 《眼科学报》 CAS 2024年第5期234-245,共12页
目的:建立并评估儿童Ⅱ期人工晶状体(intraocular lens,IOL)植入术后青光眼相关不良事件(glaucomarelated adverse events,GRAEs)的预测模型。方法:选取于中山大学中山眼科中心行Ⅱ期IOL植入术的无晶状体眼患儿205例(356眼),并在术后对... 目的:建立并评估儿童Ⅱ期人工晶状体(intraocular lens,IOL)植入术后青光眼相关不良事件(glaucomarelated adverse events,GRAEs)的预测模型。方法:选取于中山大学中山眼科中心行Ⅱ期IOL植入术的无晶状体眼患儿205例(356眼),并在术后对其随访3年。采用Cox比例风险模型确定GRAEs的预测因子,并建立列线图预测模型。采用随时间变化的受试者工作特征(receiver operating characteristic,ROC)曲线、决策曲线分析、Kaplan-Meier曲线评估模型性能,并通过Bootstrapping的C指数和校准图进行内部验证。结果:行Ⅱ期IOL植入术时年龄较大(HR=1.50,95%CI:1.03~2.19)、术后一过性高眼压(HR=9.06,95%CI:2.97~27.67)和IOL睫状沟植入术(HR=14.55,95%CI:2.11~100.57)是GRAEs的危险因素(均P<0.05),并据此建立了两个列线图预测模型。在术后1、2、3年,模型1的ROC曲线下面积(area under curve,AUC)分别为0.747(95%CI:0.776~0.935)、0.765(95%CI:0.804~0.936)和0.748(95%CI:0.736~0.918),模型2的AUC分别为0.881(95%CI:0.836~0.926)、0.895(95%CI:0.852~0.938)和0.848(95%CI:0.752~0.945)。在内部验证和评价中,两种模型均表现出良好的性能和临床净效益。Kaplan-Meier曲线显示两个不同的风险组在两个模型中都能被显著且稳健地区分。此外,本研究也构建了在线风险计算器。结论:两种列线图均能灵敏、准确地识别Ⅱ期IOL植入术后GRAEs的高危患儿,有助对其进行早期识别和及时干预。 展开更多
关键词 儿童健康(儿科) 青光眼 晶状体和悬韧带 视力
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Establishment of a system for screening and identification of novel bactericide targets in the plant pathogenic bacterium Xanthomonas oryzae pv. oryzae using Tn-seq and SPR
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作者 Chaoyue Pang ling jin +9 位作者 Haoyu Zang Damalk Saint-Claire SKoklannou Jiazhi Sun Jiawei Yang Yongxing Wang Liang Xu Chunyan Gu Yang Sun Xing Chen Yu Chen 《Journal of Integrative Agriculture》 SCIE CAS CSCD 2024年第5期1580-1592,共13页
Xanthomonas spp. cause severe bacterial diseases. However, effective strategies for prevention and management of these diseases are scarce. Thus, it is necessary to improve the efficiency of control of diseases caused... Xanthomonas spp. cause severe bacterial diseases. However, effective strategies for prevention and management of these diseases are scarce. Thus, it is necessary to improve the efficiency of control of diseases caused by Xanthomonas. In this study, Xanthomonas oryzae pv. oryzae(Xoo), which causes rice bacterial leaf blight, has been studied as a representative. A transposon insertion library of Xoo, comprising approximately 200,000 individual insertion mutants, was generated. Transposon sequencing data indicated that the mariner C9 transposase mapped at 35.7–36.4% of all potential insertion sites, revealing 491 essential genes required for the growth of Xoo in rich media. The results show that, compared to the functions of essential genes of other bacteria, the functions of some essential genes of Xoo are unknown, 25 genes might be dangerous for the Xanthomonas group, and 3 are specific to Xanthomonas. High-priority candidates for developing broad-spectrum, Xanthomonas-specific, and environment-friendly bactericides were identified in this study. In addition, this study revealed the possible targets of dioctyldiethylenetriamine using surface plasmon resonance(SPR) in combination with high performance liquid chromatography–mass spectrometry(HPLC–MS). The study also provided references for the research of some certain bactericides with unknown anti-bacterial mode of action. In conclusion, this study urged a better understanding of Xanthomonas,provided meaningful data for the management of bacterial leaf blight, and disclosed selected targets of a novel bactericide. 展开更多
关键词 rice bacterial leaf blight XANTHOMONAS essential genes Tn-seq dioctyldiethylenetriamine
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Effectiveness and safety of early lens extraction during par plana vitrectomy for proliferative diabetes retinopathy with mild cataract:a randomized clinical trial
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作者 Wei-Bo Feng Lei Zheng +17 位作者 Ying-Qi Li Yong-Hao Li Guo-Ming Zhang Xian Wang Bing-Qian Liu ling jin Yi-Nuo Huang Yang-Fan Yang Zi-Dong Chen Da-Hui Ma Qing-Shan Chen Chao-Jun Qin Bing-Min Feng Zhu-Min Yang Xing Huang Cheng-Jie Yang Sheng-Hui Liu Ming-Xing Wu 《International Journal of Ophthalmology(English edition)》 SCIE CAS 2024年第3期528-536,共9页
●AIM:To evaluate the effectiveness and safety of early lens extraction during pars plana vitrectomy(PPV)for proliferative diabetic retinopathy(PDR)compared to those of PPV with subsequent cataract surgery.●METHODS:T... ●AIM:To evaluate the effectiveness and safety of early lens extraction during pars plana vitrectomy(PPV)for proliferative diabetic retinopathy(PDR)compared to those of PPV with subsequent cataract surgery.●METHODS:This multicenter randomized controlled trial was conducted in three Chinese hospitals on patients with PDR,aged>45y,with mild cataracts.The participants were randomly assigned to the combined(PPV combined with simultaneously cataract surgery,i.e.,phacovitrectomy)or subsequent(PPV with subsequent cataract surgery 6mo later)group and followed up for 12mo.The primary outcome was the change in best-corrected visual acuity(BCVA)from baseline to 6mo,and the secondary outcomes included complication rates and medical expenses.●RESULTS:In total,129 patients with PDR were recruited and equally randomized(66 and 63 in the combined and subsequent groups respectively).The change in BCVA in the combined group[mean,36.90 letters;95%confidence interval(CI),30.35–43.45]was significantly better(adjusted difference,16.43;95%CI,8.77–24.08;P<0.001)than in the subsequent group(mean,22.40 letters;95%CI,15.55–29.24)6mo after the PPV,with no significant difference between the two groups at 12mo.The overall surgical risk of two sequential surgeries was significantly higher than that of the combined surgery for neovascular glaucoma(17.65%vs 3.77%,P=0.005).No significant differences were found in the photocoagulation spots,surgical time,and economic expenses between two groups.In the subsequent group,the duration of work incapacity(22.54±9.11d)was significantly longer(P<0.001)than that of the combined group(12.44±6.48d).●CONCLUSION:PDR patients aged over 45y with mild cataract can also benefit from early lens extraction during PPV with gratifying effectiveness,safety and convenience,compared to sequential surgeries. 展开更多
关键词 lens extraction pars plana vitrectomy proliferative diabetic retinopathy CATARACT simultaneously operations
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Classification of congenital cataracts based on multidimensional phenotypes and its association with visual outcomes
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作者 Yuan Tan Ying-Shi Zou +8 位作者 Ying-Lin Yu Le-Yi Hu Ting Zhang Hui Chen ling jin Duo-Ru Lin Yi-Zhi Liu Hao-Tian Lin Zhen-Zhen Liu 《International Journal of Ophthalmology(English edition)》 SCIE CAS 2024年第3期473-479,共7页
●AIM:To establish a classification for congenital cataracts that can facilitate individualized treatment and help identify individuals with a high likelihood of different visual outcomes.●METHODS:Consecutive patient... ●AIM:To establish a classification for congenital cataracts that can facilitate individualized treatment and help identify individuals with a high likelihood of different visual outcomes.●METHODS:Consecutive patients diagnosed with congenital cataracts and undergoing surgery between January 2005 and November 2021 were recruited.Data on visual outcomes and the phenotypic characteristics of ocular biometry and the anterior and posterior segments were extracted from the patients’medical records.A hierarchical cluster analysis was performed.The main outcome measure was the identification of distinct clusters of eyes with congenital cataracts.●RESULTS:A total of 164 children(299 eyes)were divided into two clusters based on their ocular features.Cluster 1(96 eyes)had a shorter axial length(mean±SD,19.44±1.68 mm),a low prevalence of macular abnormalities(1.04%),and no retinal abnormalities or posterior cataracts.Cluster 2(203 eyes)had a greater axial length(mean±SD,20.42±2.10 mm)and a higher prevalence of macular abnormalities(8.37%),retinal abnormalities(98.52%),and posterior cataracts(4.93%).Compared with the eyes in Cluster 2(57.14%),those in Cluster 1(71.88%)had a 2.2 times higher chance of good best-corrected visual acuity[<0.7 logMAR;OR(95%CI),2.20(1.25–3.81);P=0.006].●CONCLUSION:This retrospective study categorizes congenital cataracts into two distinct clusters,each associated with a different likelihood of visual outcomes.This innovative classification may enable the personalization and prioritization of early interventions for patients who may gain the greatest benefit,thereby making strides toward precision medicine in the field of congenital cataracts. 展开更多
关键词 CLASSIFICATION congenital cataract PHENOTYPE visual acuity cluster analysis
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Effects of Shenling Baizhu powder on endoplasmic reticulum stress related signaling pathway in liver tissues of nonalcoholic fatty liver disease rats
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作者 ling jin Mao-Xing Pan +4 位作者 Yuan-Jun Deng Xue-Hua Luo Li Han Yu-Pei Zhang Qin-He Yang 《Traditional Medicine Research》 2022年第1期21-27,共7页
Background:According to our previous studies,Shenling Baizhu powder has an excellent preventive and therapeutic effect on nonalcoholic fatty liver disease,but the prevention mechanism is still not clear.In this study,... Background:According to our previous studies,Shenling Baizhu powder has an excellent preventive and therapeutic effect on nonalcoholic fatty liver disease,but the prevention mechanism is still not clear.In this study,we intended to explore the effects of Shenling Baizhu powder on the endoplasmic reticulum stress related signaling pathway in nonalcoholic fatty liver disease rats’liver tissues.Methods:After 16 weeks,the levels of serum total cholesterol,triglyceride,high-density lipoprotein cholesterol,low-density lipoprotein cholesterol,alanine transaminase and aspartate transaminase were evaluated by an automatic biochemical analyzer,and the levels of serum free fatty acid and hepatic total cholesterol and triglyceride were evaluated by commercial kits.Then,histological changes of the liver were observed by hematoxylin and eosin and oil red-O staining.Protein expression related to the liver unfolded protein response signalling pathway was assessed using Western blot analysis.Results:The results showed that Shenling Baizhu powder supplementation reduced serum total cholesterol,triglyceride,free fatty acid,alanine transaminase,and aspartate transaminase(P<0.05 or P<0.01),as well as the levels of hepatic total cholesterol and triglyceride(P<0.01).Pathological examination showed that Shenling Baizhu powder improved hepatic steatosis and lipid accumulation.The results of biochemical parameters and histological changes indicated that Shenling Baizhu powder administration exerted protective effects against nonalcoholic fatty liver disease.In addition,Shenling Baizhu powder decreased the protein expression levels of binding immunoglobulin protein,activating transcription factor 6,phosphorylation of eukaryotic initiation factor 2 alpha,protein kinase RNA-like endoplasmic reticulum kinase and X-box binding protein 1s in the liver(P<0.05 or P<0.01).Conclusion:Shenling Baizhu powder can ameliorate high-fat diet-induced liver lipid metabolism disorder in nonalcoholic fatty liver disease rats.The mechanisms may be related to the inhibition of the expression of proteins related to unfolded protein response signaling pathways in endoplasmic reticulum stress. 展开更多
关键词 nonalcoholic fatty liver disease Shenling Baizhu powder endoplasmic reticulum stress unfolded protein response signaling pathways
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小腹主动脉瘤药物治疗的研究进展
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作者 凌晋 王伟 《临床外科杂志》 2023年第12期1120-1123,共4页
腹主动脉瘤(abdominal aortic aneurysm,AAA)是一种慢性主动脉疾病,以动脉壁的局灶性扩张与突发性破裂为特点。AAA通常没有症状,然而一旦破裂,病死率极高。通常腹主动脉最大直径≥3.0 cm或其扩张超过正常直径的1.5倍可诊断为AAA。目前AA... 腹主动脉瘤(abdominal aortic aneurysm,AAA)是一种慢性主动脉疾病,以动脉壁的局灶性扩张与突发性破裂为特点。AAA通常没有症状,然而一旦破裂,病死率极高。通常腹主动脉最大直径≥3.0 cm或其扩张超过正常直径的1.5倍可诊断为AAA。目前AAA依赖于手术治疗或是随访监测,尽管包括开放手术和腔内修复在内的手术治疗可以有效防止AAA的扩张和破裂,但也存在一定的风险和局限性,如手术费用高昂、术后感染、内漏、截瘫、穿刺点假性动脉瘤形成等。国外指南推荐以>5.5 cm(男)或5 cm(女)作为AAA病人的外科手术治疗指征[1];而瘤体直径小于上述尺寸的AAA(5.5 cm(男)或5 cm(女))称为小腹主动脉瘤(small abdominal aortic aneurysm,sAAA)。 展开更多
关键词 腹主动脉瘤 药物 治疗
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Integrative analysis of the metabolome and transcriptome reveals the potential mechanism of fruit flavor formation in wild hawthorn(Crataegus chungtienensis)
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作者 Xien Wu Dengli Luo +5 位作者 Yingmin Zhang ling jin M.James C.Crabbe Qin Qiao Guodong Li Ticao Zhang 《Plant Diversity》 SCIE CAS CSCD 2023年第5期590-600,共11页
Hawthorns are important medicinal and edible plants with a long history of health protection in China.Besides cultivated hawthorn,other wild hawthorns may also have excellent medicinal and edible value,such as Crataeg... Hawthorns are important medicinal and edible plants with a long history of health protection in China.Besides cultivated hawthorn,other wild hawthorns may also have excellent medicinal and edible value,such as Crataegus chungtienensis,an endemic species distributed in the Southwest of China.In this study,by integrating the flavor-related metabolome and transcriptome data of the ripening fruit of C.chungtienensis,we have developed an understanding of the formation of hawthorn fruit quality.The results show that a total of 849 metabolites were detected in the young and mature fruit of C.chungtienensis,of which flavonoids were the most detected metabolites.Among the differentially accumulated metabolites,stachyose,maltotetraose and cis-aconitic acid were significantly increased during fruit ripening,and these may be important metabolites affecting fruit flavor change.Moreover,several flavonoids and terpenoids were reduced after fruit ripening compared with young fruit.Therefore,using the unripe fruit of C.chungtienensis may allow us to obtain more medicinal active ingredients such as flavonoids and terpenoids.Furthermore,we screened out some differentially expressed genes(DEGs)related to fruit quality formation,which had important relationships with differentially accumulated sugars,acids,flavonoids and terpenoids.Our study provides new insights into flavor formation in wild hawthorn during fruit development and ripening,and at the same time this study lays the foundation for the improvement of hawthorn fruit flavor. 展开更多
关键词 HAWTHORN Crataegus chungtienensis FLAVONOIDS TERPENOIDS Fruit quality
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Posterior scleral application of a mitomycin C-soaked sponge during trabeculectomy
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作者 Kun Hu Yun-He Song +5 位作者 Feng-Bin Lin Ying-Zhe Zhang ling jin Meng-Yin Liang Robert N.Weinreb Xiu-Lan Zhang 《International Journal of Ophthalmology(English edition)》 SCIE CAS 2023年第7期1071-1077,共7页
AIM:To evaluate the safety and efficacy of posterior scleral application(a modified technique)of an antimetabolite mitomycin C-soaked sponge in trabeculectomy for patients with glaucoma.METHODS:This retrospective stud... AIM:To evaluate the safety and efficacy of posterior scleral application(a modified technique)of an antimetabolite mitomycin C-soaked sponge in trabeculectomy for patients with glaucoma.METHODS:This retrospective study included 101 patients(115 eyes)with glaucoma(aged 12–83y)who underwent trabeculectomy using a modified mitomycin C-soaked sponge placement method.A piece of 3.5×10 mm2 sponge was placed vertically and posteriorly with the long side perpendicular to the limbus.The mitomycin C concentration and exposure time were 0.2–0.5 mg/m L and 1–5min,respectively.Intraocular pressure,bestcorrected visual acuity,and hypotensive medications were recorded at baseline and at the final visit.Complications,interventions required,and bleb morphology were recorded postoperatively.The primary outcome was trabeculectomy safety,including complications and bleb morphology;the secondary outcome was the trabeculectomy success rate.RESULTS:At the final follow-up[median 28mo,range 7–67mo and interquartile range(IQR)13mo],the qualified(cumulative)success rate was 93.0%and the complete success rate was 60.0%.No bleb-related complications were observed.The mean height,extent,and vascularity grades were 0.6±0.9,1.1±0.4,and 2.4±0.9,respectively.All Seidel tests were negative.The mean posteriority grade was 0.8±0.4.CONCLUSION:Trabeculectomy with the long side of a mitomycin C-soaked sponge placed perpendicular to the corneal limbus is safe and effective. 展开更多
关键词 GLAUCOMA mitomycin C posterior scleral application TRABECULECTOMY bleb morphology
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提升会议视频与第三方会议终端互联画面质量方法
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作者 凌进 程伟华 吴小虎 《微型电脑应用》 2023年第7期173-176,共4页
针对会议视频展示过程中第三方用户体验不佳、清晰度较低的问题,通过全参考(FR)评价模型对视频画面分辨率进行数据评价,利用两阶段盲卷积(TBCD)技术进行降噪处理,使终端互联画面质量得到提升。利用平滑L0范数的重建(SL0)算法把握视频画... 针对会议视频展示过程中第三方用户体验不佳、清晰度较低的问题,通过全参考(FR)评价模型对视频画面分辨率进行数据评价,利用两阶段盲卷积(TBCD)技术进行降噪处理,使终端互联画面质量得到提升。利用平滑L0范数的重建(SL0)算法把握视频画面清晰度,并计算视频信号展示标准,使画面质量最大化。通过SolidWorks软件仿真会议视频演示过程,实验表明此设计对会议视频质量提升具有明显效果,信噪比最高为39.7 dB,视频画面分辨率为2560×1600 dpi,视频演示清晰度为98.4%。 展开更多
关键词 第三方会议视频 终端互联画面 FR模型 TBCD技术 SL0算法
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白内障患者术后角膜内皮细胞计数减少的多因素分析 被引量:24
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作者 曹端荣 龚静青 +2 位作者 金玲 牛静宜 牛玉玲 《国际眼科杂志》 CAS 北大核心 2018年第4期716-719,共4页
目的:探讨白内障患者超声乳化术后影响角膜内皮细胞数目减少的相关因素。方法:选取我院眼科采用超声乳化术治疗的98例120眼进行回顾性分析,收集时间2014-07/2016-07;根据患者术后2mo复查角膜中央内皮细胞密度与术前进行比较分为严重丢失... 目的:探讨白内障患者超声乳化术后影响角膜内皮细胞数目减少的相关因素。方法:选取我院眼科采用超声乳化术治疗的98例120眼进行回顾性分析,收集时间2014-07/2016-07;根据患者术后2mo复查角膜中央内皮细胞密度与术前进行比较分为严重丢失组52例67眼(角膜中央内皮细胞丢失率≥12.3%),一般丢失组46例53眼(角膜中央内皮细胞丢失率<12.3%),对比两组患者的一般资料、手术相关指标,采用非条件Logistic回归分析法探讨影响白内障患者术后角膜内皮细胞丢失的影响因素。结果:严重丢失组和一般丢失组的性别、合并高血压、合并糖尿病、合并高血脂、浅前房、角膜直径增大、注吸时间比较,差异均无统计学意义(P>0.05);两组比较的Emery晶状体核硬度分级、超声能量、超声乳化时间、年龄构成差异具有统计学意义(P<0.05);采用非条件Logistic分析法,结果显示Emery晶状体核硬度分级增高、超声能量增加、超声乳化时间较长、年龄增大是超声乳化术治疗后角膜内皮细胞数目严重减少的独立危险因素(P<0.05)。结论:白内障患者超声乳化术后影响角膜内皮细胞数目减少的主要因素为Emery晶状体核硬度分级增高、超声能量增加、超声乳化时间较长、年龄增大。 展开更多
关键词 白内障 超声乳化术 角膜内皮细胞 相关因素
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低浓度阿托品联合角膜塑形镜治疗青少年中低度近视的疗效 被引量:40
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作者 牛玉玲 叶茹珊 +2 位作者 邓铤明 金玲 牛静宜 《国际眼科杂志》 CAS 北大核心 2019年第11期1940-1944,共5页
目的:分析角膜塑形镜联合低浓度阿托品对青少年中低度近视的控制效果。方法:选取2016-01/06我科收治的中低度近视青少年患者76例152眼,按照自愿原则分为角膜塑形组36例72眼与角膜塑形镜联合低浓度阿托品组40例80眼(联合组),两组患者均... 目的:分析角膜塑形镜联合低浓度阿托品对青少年中低度近视的控制效果。方法:选取2016-01/06我科收治的中低度近视青少年患者76例152眼,按照自愿原则分为角膜塑形组36例72眼与角膜塑形镜联合低浓度阿托品组40例80眼(联合组),两组患者均配戴角膜塑形镜,联合组加用0.1g/L阿托品滴眼液点眼,均随访1a,检查戴镜前后裸眼视力、屈光度、眼轴长度、眼压、泪膜功能及角膜各参数的变化,统计并发症发生率。结果:戴镜前、戴镜1a两组裸眼视力、屈光度、眼轴长度、眼压比较无差异(P>0.05),联合组戴镜前、戴镜1a裸眼视力变化幅度高于角膜塑形镜组,屈光度及眼轴长度差值低于角膜塑形镜组(P<0.05);戴镜前,两组患者泪膜脂质层厚度(LLT)、客观散射指数(OSI)、泪膜破裂时间(BUT)比较无差异(P>0.05),戴镜1a,联合组LLT、BUT高于角膜塑形镜组,OSI低于角膜塑形镜组(P<0.05);戴镜前,两组患者角膜曲率、角膜厚度、前房深度比较无差异(P>0.05),戴镜1a,联合组角膜曲率、角膜厚度、前房深度变化幅度均低于角膜塑形镜组(P<0.05);两组患者并发症发生率比较无差异(P>0.05)。结论:角膜塑形镜联合低浓度阿托品可改善青少年中低度近视患者裸眼视力,控制屈光度、眼轴增长,对角膜形态及泪膜功能无明显负面影响,安全性高。 展开更多
关键词 中低度近视 角膜塑形镜 阿托品 视力
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新冠疫情期间眼病患者线上问诊模式与影响因素分析 被引量:4
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作者 刘臻臻 吴晓航 +6 位作者 陈睛晶 晏丕松 金玲 胡伟玲 张恩恩 李伟 林浩添 《国际眼科杂志》 CAS 北大核心 2020年第11期1996-2002,共7页
目的:新型冠状病毒疫情期间,中山大学中山眼科中心在严格防控疫情的前提下及时开展免费线上“专家问诊”服务,眼科医师在线答疑以满足眼病诊疗需求。本研究对疫情期间眼科患者线上问诊的需求与模式进行分析,为眼科互联网诊疗体系的优化... 目的:新型冠状病毒疫情期间,中山大学中山眼科中心在严格防控疫情的前提下及时开展免费线上“专家问诊”服务,眼科医师在线答疑以满足眼病诊疗需求。本研究对疫情期间眼科患者线上问诊的需求与模式进行分析,为眼科互联网诊疗体系的优化提供依据。方法:前瞻性观察性研究。本研究分析了2020-02-01/03-15期间,使用中山眼科中心互联网医院“专家问诊”服务患者的基线特征。针对其中重复问诊的患者,随机抽取等量单次问诊患者作为对照,比较单次问诊与重复问诊患者的基线特征、就诊目的、疾病类型、眼病数量,并对患者问诊次数的影响因素进行分析。结果:研究期间“专家问诊”共线上服务患者9831人次,其中单次问诊3919人次,5912人次来源于1967人的重复问诊。随机抽取1967名单次问诊者与上述重复问诊者对比,单次问诊与重复问诊患者的年龄、性别分布无统计学差异(P=0.897,0.482)。两组就诊目的均以“旧病复诊”占比最高(59.7%,64.9%)。从构成比上看,与单次问诊患者相比,重复问诊患者中,就诊目的为新发不适者较少,旧病复诊与术后复查者较多,眼表、眼底、葡萄膜疾病患者较多,患有两种及以上眼病者占比较多。回归分析显示屈光患者倾向单次问诊,而旧病复诊、术后短期复诊患者,眼底、葡萄膜疾病患者倾向重复问诊。旧病复诊、术后随访较新发不适患者重复问诊的次数更多;晶状体与青光眼/疑似青光眼疾病重复问诊次数较少,眼表疾病重复问诊次数较多。结论:疫情期间眼科患者线上就诊主要目的为旧病复诊。疾病种类、就诊目的与眼病数量均影响患者是否重复问诊及问诊次数,应根据患者的就诊需求与模式调整眼科不同亚专科线上接诊医生的数量与排班。互联网医疗对新发不适患者的诊疗能力有待进一步提高。 展开更多
关键词 新型冠状病毒引起的传染性疾病 流行病 互联网医疗
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不同年龄段慢性感染性角膜炎的病因和病原学特征分析 被引量:4
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作者 曹端荣 龚静青 +1 位作者 金玲 罗治 《国际眼科杂志》 CAS 北大核心 2018年第9期1681-1683,共3页
目的:探讨不同年龄段慢性感染性角膜炎的病因和病原学特征。方法:将我院2015-01/2017-12确诊为慢性感染性角膜炎患者89例89眼作为研究对象,根据年龄分为13~32岁组35例35眼、33~52岁组28例28眼、53~73岁组26例26眼,对其不同年龄段的... 目的:探讨不同年龄段慢性感染性角膜炎的病因和病原学特征。方法:将我院2015-01/2017-12确诊为慢性感染性角膜炎患者89例89眼作为研究对象,根据年龄分为13~32岁组35例35眼、33~52岁组28例28眼、53~73岁组26例26眼,对其不同年龄段的发病病因和病原学特征进行回顾性研究。结果:三组患者在病因分布上差异无统计学意义(P=0.290);三组患者在微生物组成分布上差异无统计学意义(χ~2=0.258,P=1.000)。三组患者真菌感染导致的慢性感染性角膜炎发生率相较于其他致病微生物均较高;所有患者中,细菌感染培养阳性患者26例,其中表皮葡萄球菌50%、绿脓杆菌15%、唾液链球菌12%、肺炎链球菌8%、大肠埃希菌8%和淋病双球菌8%,绿脓杆菌和表皮葡萄球菌是阳性培养结果中常见的细菌菌株;在真菌菌种感染患者中24例为培养阳性,其中镰孢菌属46%、曲霉菌属17%、交链孢属21%、菌丝体(无孢菌)4%、青霉菌属4%和弯孢菌属8%,所有阳性患者中以镰孢菌属和交链孢属两种菌种较高。结论:感染性角膜炎在不同年龄段的发病原因没有显著差异,在病原学分布上主要以真菌感染为主,病原学特征上细菌以表皮葡萄球菌为主,而真菌以镰孢菌属为主,应加强对感染性角膜炎的早期预防和防范。 展开更多
关键词 不同年龄 感染性角膜炎 病因 病原学特征
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4例散发性克雅病的临床分析
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作者 刘想林 刘鉴仪 +3 位作者 金玲 高云春 洪诗英 朱建明 《阿尔茨海默病及相关病杂志》 2023年第3期230-235,共6页
目的:分析4例临床诊断为很可能散发性克雅病(sCJD)患者的临床特点,以提高对克雅病的认识。方法:回顾性分析2018年8月至2022年5月中南大学湘雅医学院附属常德医院神经内科收治的4例sCJD患者的临床资料及辅助检查结果。结果:4例患者均为... 目的:分析4例临床诊断为很可能散发性克雅病(sCJD)患者的临床特点,以提高对克雅病的认识。方法:回顾性分析2018年8月至2022年5月中南大学湘雅医学院附属常德医院神经内科收治的4例sCJD患者的临床资料及辅助检查结果。结果:4例患者均为亚急性起病,主要临床特点包括快速进展性痴呆、小脑症状、锥体束征、视觉症状、肌痉挛、无动性缄默。磁共振弥散加权像序列(DWI)可见不对称的沿皮层分布的花边样高信号、基底节曲棍球征。脑电图表现为弥漫性慢波,疾病晚期表现为周期性三相波。4例患者均行脑脊液14-3-3蛋白检查,结果均为阳性。所有患者均于发病后3月内死亡。结论:中老年患者出现快速进展性痴呆,尤其头颅磁共振DWI序列存在皮层高信号,需警惕克雅病,动态复查头颅磁共振、脑电图,脑脊液送检14-3-3蛋白,以免误诊、漏诊。 展开更多
关键词 克雅病 快速进展性痴呆 头颅磁共振(MRI) 脑电图 14-3-3蛋白
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Spacer区蛋白对ADAMTS13活性影响的研究 被引量:2
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作者 马珍妮 凌婧 +4 位作者 殷杰 沈飞 苏健 谢丽倩 阮长耿 《中国实验血液学杂志》 CAS CSCD 北大核心 2019年第5期1596-1601,共6页
目的:本研究旨在获得血管性血友病因子裂解酶ADAMTS13中重组的spacer区蛋白,进一步探讨该区域对ADAMTS13功能发挥的作用。方法:以ADAMTS13全长质粒为模板,构建含spacer区基因序列的原核表达质粒,转染大肠杆菌,低温(30℃)IPTG诱导表达,... 目的:本研究旨在获得血管性血友病因子裂解酶ADAMTS13中重组的spacer区蛋白,进一步探讨该区域对ADAMTS13功能发挥的作用。方法:以ADAMTS13全长质粒为模板,构建含spacer区基因序列的原核表达质粒,转染大肠杆菌,低温(30℃)IPTG诱导表达,收集超声上清并鉴定。并大规模诱导表达,利用Ni-NTA琼脂糖柱,梯度咪唑淋洗法纯化蛋白,应用SDS-PAGE和Western blot法鉴定纯化产品纯度和免疫学活性。利用重组蛋白与免疫性调节的血栓性血小板减少性紫癜(iTTP)患者血浆孵育,检测血浆中ADAMTS13的活性变化。结果:成功构建能表达spacer区的原核表达质粒并能有效表达蛋白。Western blotting结果显示,抗6×His抗体和抗人ADAMTS13抗体(针对Gln34-Trp688)分别能与重组蛋白在15 kDa处显单一条带。功能实验表明,该重组蛋白能够有效逆转iTTP患者中被自身抗体抑制的ADAMTS13酶活性。结论:重组蛋白具有较好的免疫原活性和纯度,为进一步研究spacer区对iTTP的发病机理提供了实验依据。 展开更多
关键词 spacer区域 血管性血友病因子裂解酶 原核表达
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照相机辅助测量在测定共同性斜视斜视角中的应用 被引量:2
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作者 牛玉玲 叶茹珊 +2 位作者 金玲 邓铤明 牛静宜 《国际眼科杂志》 CAS 北大核心 2019年第8期1381-1384,共4页
目的:探讨照相机辅助测量对评估角膜映光法(Hirschberg Test)测定共同性斜视斜视角的准确性。方法:收集2016-01/2018-01我院收治的105例共同性斜视患者的临床资料,入院后均接受传统Hirschberg Test法、图片Hirschberg Test法斜视角检查... 目的:探讨照相机辅助测量对评估角膜映光法(Hirschberg Test)测定共同性斜视斜视角的准确性。方法:收集2016-01/2018-01我院收治的105例共同性斜视患者的临床资料,入院后均接受传统Hirschberg Test法、图片Hirschberg Test法斜视角检查,以三棱镜遮盖试验为金标准,记录传统Hirschberg Test法、图片Hirschberg Test法与金标准测定斜视角偏差值,分析Kappa角对偏差值的影响,分析照相机辅助测量在共同性斜视角中的应用价值。结果:传统Hirschberg Test法、图片Hirschberg Test法、三棱镜遮盖试验测定斜视角比较差异均有统计学意义(P<0.05),三棱镜遮盖试验测定斜视角度高于图片Hirschberg Test法(P<0.05),图片Hirschberg Test法又高于传统Hirschberg Test法(P<0.05),图片Hirschberg Test法与三棱镜遮盖试验测定斜视角一致性高于传统Hirschberg Test法(P<0.05);传统Hirschberg Test法测定斜视角偏差值比较差异有统计学意义(P<0.05),传统Hirschberg Test法偏差值均高于图片Hirschberg Test法(P<0.05);图片Hirschberg Test法对阳性、阴性Kappa角患者倾斜度检查偏差度均低于传统Hirschberg Test法(P<0.05)。结论:照相机辅助测量图片Hirschberg Test法对共同性斜视斜视角测定偏差低于传统Hirschberg Test法,且更适用于无法配合的低龄患者。 展开更多
关键词 共同性斜视 照相机 角膜映光法 三棱镜 斜视角 图片
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14-3-3 zeta蛋白过表达与他莫昔芬治疗乳腺癌耐药的关系 被引量:4
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作者 凌人 凌今 +3 位作者 陈仲华 刘丹丹 赵宏圻 柯友涛 《温州医科大学学报》 CAS 2017年第3期192-196,共5页
目的:探讨14-3-3 zeta蛋白过表达与他莫昔芬治疗乳腺癌耐药的关系。方法:采用免疫组织化学S-P法对80例雌激素受体阳性乳腺癌组织中14-3-3 zeta蛋白表达进行检测,根据14-3-3 zeta蛋白表达情况将患者分组,结合每组生存情况评价14-3-3 zet... 目的:探讨14-3-3 zeta蛋白过表达与他莫昔芬治疗乳腺癌耐药的关系。方法:采用免疫组织化学S-P法对80例雌激素受体阳性乳腺癌组织中14-3-3 zeta蛋白表达进行检测,根据14-3-3 zeta蛋白表达情况将患者分组,结合每组生存情况评价14-3-3 zeta蛋白过表达与他莫昔芬治疗乳腺癌耐药的关系。结果:癌组织中14-3-3 zeta蛋白表达总阳性率为96.25%(77/80),其中强阳性占51.25%(41/80),中等阳性占23.75%(19/80),弱阳性占21.25%(17/80)。14-3-3 zeta蛋白过表达与临床病理因素无明显相关性。随访结果显示,14-3-3 zeta蛋白过表达组患者在应用他莫昔芬治疗后更容易复发,生存期明显较短。细胞实验表明,提高乳腺癌细胞中14-3-3 zeta蛋白表达可以增强其对他莫昔芬的耐药性,反之,抑制14-3-3 zeta蛋白表达可有效提高他莫昔芬治疗的敏感性。结论:14-3-3 zeta蛋白可以作为他莫昔芬治疗乳腺癌耐药评估的生物标记物,检测乳腺癌组织内14-3-3 zeta蛋白表达可以用于评估他莫昔芬治疗乳腺癌的疗效。 展开更多
关键词 乳腺肿瘤 14-3-3zeta 免疫组织化学法 他莫昔芬 细胞凋亡
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Berberine Ameliorates High-Fat Diet-Induced Non-Alcoholic Fatty Liver Disease in Rats via Activation of SIRT3/AMPK/ACC Pathway 被引量:26
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作者 Yu-pei ZHANG Yuan-jun DENG +8 位作者 Kai-rui TANG Run-sen CHEN Shu LIANG Yin-ji LIANG Li HAN ling jin Zi-en LIANG Yan-ning CHEN Qin-he YANG 《Current Medical Science》 SCIE CAS 2019年第1期37-43,共7页
This study aimed to verify the effects of berberine(BBR)on the fat metabolism proteins involved in the sirtuin 3(SIRT3)/adenosine 5'-monophosphate(AMP)-activated protein kinase(AMPK)/acetyl-CoA carboxylase(ACC)pat... This study aimed to verify the effects of berberine(BBR)on the fat metabolism proteins involved in the sirtuin 3(SIRT3)/adenosine 5'-monophosphate(AMP)-activated protein kinase(AMPK)/acetyl-CoA carboxylase(ACC)pathway in the liver tissues of rats with high-fat diet(HFD)-induced non-alcoholic fatty liver disease(NAFLD).Forty-eight rats were randomly divided into the normal control(NC)group,HFD group or BBR group,with 16 rats in each group.After 8 and 16 weeks of treatment,serum and liver samples were collected.Subsequently,body parameters,biochemical parameters and liver pathology were examined.The expression levels of proteins involved in the SIRT3/AMPK/ACC pathway in the liver were detected by Western blotting.After 8 and 16 weeks of a HFD,the successful establishment of rat models with different degrees of NAFLD was confirmed by hematoxylin and eosin(H&E)and Oil Red O staining.NAFLD rat models exhibited obesity and hyperlipidemia,and the protein expression levels of SIRT3,p-AMPK.p-ACC,and CPT-1A in the liver were significantly decreased compared to those in the NC group.The concurrent administration of BBR with the HFD effectively improved serum and liver lipid profiles and ameliorated liver injury.Furthermore,the protein expression levels of SIRT3,p-AMPK,p-ACC,and CPT-1 A in the liver were significantly increased in the BBR group as compared with those in the HFD group.In conclusion,our data suggest that the mechanism by which BBR ameliorates HFD-induced hepatic steatosis may be related to the activation of the SIRT3/AMPK/ACC pathway in the liver. 展开更多
关键词 BERBERINE non-alcoholic FATTY liver disease SIRTUIN 3 LIPID METABOLISM
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志愿精神育人的基本特征及实践路径 被引量:1
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作者 凌瑾 熊平安 《教师教育论坛》 2020年第1期49-52,共4页
志愿精神育人具有重要的现实意义,它是社会主义核心价值观的彰显,蕴含着服务学习的实践指向,有助于校园文化的品质提升。与其他的育人方式相比,志愿精神育人有着独特的基本特征,即在服务奉献中塑造优良品德、在人际互动中优化人际关系... 志愿精神育人具有重要的现实意义,它是社会主义核心价值观的彰显,蕴含着服务学习的实践指向,有助于校园文化的品质提升。与其他的育人方式相比,志愿精神育人有着独特的基本特征,即在服务奉献中塑造优良品德、在人际互动中优化人际关系、在探索世界中促进个人与社会共同进步。志愿精神育人的实践路径有:大力弘扬志愿精神,以志愿精神引领校园风尚;促进志愿服务制度化,壮大志愿服务队伍规模;挖掘志愿服务先进人物,以榜样力量发挥正能量。 展开更多
关键词 志愿精神 育人 志愿服务 大学生
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