Myostatin(MSTN)gene negatively controls skeletal muscle development and growth,variations of which play an important role in the regulation of skeletal muscle growth in mammals.However,study on genetic polymorphism of...Myostatin(MSTN)gene negatively controls skeletal muscle development and growth,variations of which play an important role in the regulation of skeletal muscle growth in mammals.However,study on genetic polymorphism of MSTN gene in donkey is limited.In this study,we screened the single nucleotide polymorphsims(SNPs)of MSTN gene in 13 Chinese donkey breeds.Four novel SNPs(g.229T>C,g.872A>G,g.2014G>A,and g.2395C>G)were detected and genotyped by sequencing and polymerase chain reaction-restriction fragment length polymorphism(PCR-RFLP)methods.Six haplotypes(H1–H6)were analyzed,which indicated abundant haplotype diversities in Chinese donkeys.The haplotype H1 was the most dominant and ancient in all breeds.Xinjiang donkey displayed the highest haplotype diversity.The Neighbour-Joining(NJ)tree of MSTN gene among different species was constructed.The clustering result of nine species was consistent with the fact of species differentiation.Our results will provide a reliable theoretical basis for the preservation,exploration and utilization of Chinese donkey genetic resources.展开更多
The effects of different concentrations of copper sulfate on root and shoot growth of maize( Zea mays L.) and the uptake and accumulation of Cu 2+ by its roots and shoots were investigated in the present study. The co...The effects of different concentrations of copper sulfate on root and shoot growth of maize( Zea mays L.) and the uptake and accumulation of Cu 2+ by its roots and shoots were investigated in the present study. The concentrations of copper sulfate (CuSO 4·5H 2O) used were in the range of 10 -5 -10 -3 mol/L. Root growth decreased progressively with increasing concentration of Cu 2+ in solution. The seedlings exposed to 10 -3 mol/L Cu 2+ exhibited substantial growth reduction, yielding only 68% of the root length of the control. The shoot growth of the seedlings grown at 10 -5 -10 -4 mol/L Cu 2+ were more or less the same as the control seedlings. The leaves treated with 10 -3 mol/L Cu 2+ were obviously inhibited in shoot growth. The fresh and dry weights both in roots and shots decreased progressively with increasing Cu 2+ concentration. This fits well with the above mentioned effects of copper sulfate on root growth. Zea mays has considerable ability to remove Cu from solutions and accumulate it. The Cu content in roots of Z.mays increased with increasing solution concentration of Cu 2+ . The amount of Cu in roots of plants treated with 10 -3 , 10 -4 and 10 -5 mol/L Cu 2+ were 10, 8 and 1 5 fold, respectively, greater than that of roots of control plant. However, the plants transported and concentrated only a small amount of Cu in their shoots.展开更多
基金supported by the National Natural Science Foundation of China (31072001, 31272399, 81270439)
文摘Myostatin(MSTN)gene negatively controls skeletal muscle development and growth,variations of which play an important role in the regulation of skeletal muscle growth in mammals.However,study on genetic polymorphism of MSTN gene in donkey is limited.In this study,we screened the single nucleotide polymorphsims(SNPs)of MSTN gene in 13 Chinese donkey breeds.Four novel SNPs(g.229T>C,g.872A>G,g.2014G>A,and g.2395C>G)were detected and genotyped by sequencing and polymerase chain reaction-restriction fragment length polymorphism(PCR-RFLP)methods.Six haplotypes(H1–H6)were analyzed,which indicated abundant haplotype diversities in Chinese donkeys.The haplotype H1 was the most dominant and ancient in all breeds.Xinjiang donkey displayed the highest haplotype diversity.The Neighbour-Joining(NJ)tree of MSTN gene among different species was constructed.The clustering result of nine species was consistent with the fact of species differentiation.Our results will provide a reliable theoretical basis for the preservation,exploration and utilization of Chinese donkey genetic resources.
基金The National Natural Science Foundation of China(No .39870 0 47) .
文摘The effects of different concentrations of copper sulfate on root and shoot growth of maize( Zea mays L.) and the uptake and accumulation of Cu 2+ by its roots and shoots were investigated in the present study. The concentrations of copper sulfate (CuSO 4·5H 2O) used were in the range of 10 -5 -10 -3 mol/L. Root growth decreased progressively with increasing concentration of Cu 2+ in solution. The seedlings exposed to 10 -3 mol/L Cu 2+ exhibited substantial growth reduction, yielding only 68% of the root length of the control. The shoot growth of the seedlings grown at 10 -5 -10 -4 mol/L Cu 2+ were more or less the same as the control seedlings. The leaves treated with 10 -3 mol/L Cu 2+ were obviously inhibited in shoot growth. The fresh and dry weights both in roots and shots decreased progressively with increasing Cu 2+ concentration. This fits well with the above mentioned effects of copper sulfate on root growth. Zea mays has considerable ability to remove Cu from solutions and accumulate it. The Cu content in roots of Z.mays increased with increasing solution concentration of Cu 2+ . The amount of Cu in roots of plants treated with 10 -3 , 10 -4 and 10 -5 mol/L Cu 2+ were 10, 8 and 1 5 fold, respectively, greater than that of roots of control plant. However, the plants transported and concentrated only a small amount of Cu in their shoots.
