【目的】筛选金边也门铁花叶性状相关差异表达基因,为深入探索也门铁叶色形成的分子调控机制打下基础。【方法】基于cDNA扩增片段长度多态性分析技术(cDNA-AFLP),利用64对Eco R I和Mse I选择性扩增引物组合分析金边也门铁叶片绿色与黄...【目的】筛选金边也门铁花叶性状相关差异表达基因,为深入探索也门铁叶色形成的分子调控机制打下基础。【方法】基于cDNA扩增片段长度多态性分析技术(cDNA-AFLP),利用64对Eco R I和Mse I选择性扩增引物组合分析金边也门铁叶片绿色与黄色部分组织cDNA,经筛选、回收、克隆、测序和BLAST比对分析获得差异表达基因。【结果】利用64对引物组合共扩增出1726条可分辨的条带,并获得58条差异表达的转录衍生片段(TDFs),其中12条TDFs为特异性表达。12条特异性TDFs中,5条TDFs具有同源序列,另外7条TDFs无同源序列,为未知功能基因。在叶片黄化部位特异表达同源性为96%的TDFs序列M8E3B-2和M8E3B-3与大丽花花叶病毒Holland包涵体蛋白基因、紫茎泽兰明脉病毒基因、洋丁香黄化卷叶病毒基因等花椰菜花叶病毒科病毒基因具有较高的同源性。【结论】M8E3B-2和M8E3B-3两条TDFs序列或来源于一种花椰菜花叶病毒科病毒基因,且有可能参与了金边也门铁花叶叶色性状的形成过程。展开更多
A steady three-dimensional fluid flow and solidification model was built based on CFD software by high-Reynolds-number and Lam-Bremhorst low-Reynolds-number k-ε model.During the simulation,the fixed-grid enthalpy-por...A steady three-dimensional fluid flow and solidification model was built based on CFD software by high-Reynolds-number and Lam-Bremhorst low-Reynolds-number k-ε model.During the simulation,the fixed-grid enthalpy-porosity technique was used to represent the solidification,and Darcy law was adopted to simulate the flow in mushy region.The prediction for steel flow and solidification was evaluated by the comparison of two turbulence models.It is found that both Lam-Bremhorst low-Reynolds-number and high-Reynolds-number k-ε models predict the same trend of the steel flow and temperature distribution.However,due to the effect of turbulent flow on heat transfer,the low-Reynolds-number turbulence model predicts longer penetration depth of molten steel in sub-mold region,less shell growth and higher shell surface temperature at the narrow face compared with standard k-ε model.展开更多
文摘【目的】筛选金边也门铁花叶性状相关差异表达基因,为深入探索也门铁叶色形成的分子调控机制打下基础。【方法】基于cDNA扩增片段长度多态性分析技术(cDNA-AFLP),利用64对Eco R I和Mse I选择性扩增引物组合分析金边也门铁叶片绿色与黄色部分组织cDNA,经筛选、回收、克隆、测序和BLAST比对分析获得差异表达基因。【结果】利用64对引物组合共扩增出1726条可分辨的条带,并获得58条差异表达的转录衍生片段(TDFs),其中12条TDFs为特异性表达。12条特异性TDFs中,5条TDFs具有同源序列,另外7条TDFs无同源序列,为未知功能基因。在叶片黄化部位特异表达同源性为96%的TDFs序列M8E3B-2和M8E3B-3与大丽花花叶病毒Holland包涵体蛋白基因、紫茎泽兰明脉病毒基因、洋丁香黄化卷叶病毒基因等花椰菜花叶病毒科病毒基因具有较高的同源性。【结论】M8E3B-2和M8E3B-3两条TDFs序列或来源于一种花椰菜花叶病毒科病毒基因,且有可能参与了金边也门铁花叶叶色性状的形成过程。
文摘A steady three-dimensional fluid flow and solidification model was built based on CFD software by high-Reynolds-number and Lam-Bremhorst low-Reynolds-number k-ε model.During the simulation,the fixed-grid enthalpy-porosity technique was used to represent the solidification,and Darcy law was adopted to simulate the flow in mushy region.The prediction for steel flow and solidification was evaluated by the comparison of two turbulence models.It is found that both Lam-Bremhorst low-Reynolds-number and high-Reynolds-number k-ε models predict the same trend of the steel flow and temperature distribution.However,due to the effect of turbulent flow on heat transfer,the low-Reynolds-number turbulence model predicts longer penetration depth of molten steel in sub-mold region,less shell growth and higher shell surface temperature at the narrow face compared with standard k-ε model.
