Objective Combination immunotherapy strategies targeting OX40,a co-stimulatory molecule that can enhance antitumor immunity by modulating the proliferation,differentiation,and effector function of tumor-infiltrating T...Objective Combination immunotherapy strategies targeting OX40,a co-stimulatory molecule that can enhance antitumor immunity by modulating the proliferation,differentiation,and effector function of tumor-infiltrating T cells,have attracted much attention for their excellent therapeutic effects.In this study,we aimed to evaluate the antitumor efficacy of combined anti-OX40 and hepatitis B core viruslike particles(HBc VLPs)therapy using a mouse colon cancer model.Methods Humanized B-h OX40 mice were injected subcutaneously with MC38 colon tumor cells and treated with HBc VLPs+anti-h OX40 antibody.Tumor growth was monitored.Flow cytometric analysis was performed to evaluate the populations of T cell subsets in the tumors.Results The combination of anti-OX40 with HBc VLPs resulted in a significant delay in tumor growth,suggesting that a potent antitumor immunity was induced by the combination therapy.Further studies revealed that HBc VLPs+anti-OX40 treatment induced a significant increase in effector T cells(Teffs)and a significant decrease in regulatory T cells(Tregs)in the tumor microenvironment(TME),which accounted for the synergistic antitumor effect of anti-OX40 in combination with HBc VLPs.Conclusion Combination therapy of anti-h OX40 and HBc VLPs provides synergistic antitumor activity in colon cancer-bearing mice,which may represent a potential design strategy for cancer immunotherapy.展开更多
Periodontitis is a chronic infectious disease characterized by plaque biofilm as its main pathogenic factor;its clinical manifestations include gingival inflammation,alveolar bone resorption,attachment loss,and period...Periodontitis is a chronic infectious disease characterized by plaque biofilm as its main pathogenic factor;its clinical manifestations include gingival inflammation,alveolar bone resorption,attachment loss,and periodontal pocket formation,all of which eventually lead to tooth loss and seriously affect the quality of life of patients[1].This illness has a close relationship with blood sugar levels in patients with diabetes and is one of the most important complications of diabetes[2].Macrophages are an important part of host immune responses and exhibit powerful functions of identifying,phagocytizing,and removing bacteria;macrophage polarization is involved in the pathogenesis of periodontitis[3].展开更多
基金supported by National Major Science and Technology Projects of China 2017ZX10105015-001-002。
文摘Objective Combination immunotherapy strategies targeting OX40,a co-stimulatory molecule that can enhance antitumor immunity by modulating the proliferation,differentiation,and effector function of tumor-infiltrating T cells,have attracted much attention for their excellent therapeutic effects.In this study,we aimed to evaluate the antitumor efficacy of combined anti-OX40 and hepatitis B core viruslike particles(HBc VLPs)therapy using a mouse colon cancer model.Methods Humanized B-h OX40 mice were injected subcutaneously with MC38 colon tumor cells and treated with HBc VLPs+anti-h OX40 antibody.Tumor growth was monitored.Flow cytometric analysis was performed to evaluate the populations of T cell subsets in the tumors.Results The combination of anti-OX40 with HBc VLPs resulted in a significant delay in tumor growth,suggesting that a potent antitumor immunity was induced by the combination therapy.Further studies revealed that HBc VLPs+anti-OX40 treatment induced a significant increase in effector T cells(Teffs)and a significant decrease in regulatory T cells(Tregs)in the tumor microenvironment(TME),which accounted for the synergistic antitumor effect of anti-OX40 in combination with HBc VLPs.Conclusion Combination therapy of anti-h OX40 and HBc VLPs provides synergistic antitumor activity in colon cancer-bearing mice,which may represent a potential design strategy for cancer immunotherapy.
基金supported by grants from the National Natural Science Foundation of China [No.81570945 to DING Gang]the Natural Science Foundation of Shandong Province [No.ZR2021MH051 to DING Gang+2 种基金No.ZR2020MH188 to LIU Yun Xia]2021 Youth Innovation Talent Introduction and Education Program of Shandong Province Universities (The innovative team for molecular epidemiology of oral cancer based on multiomics)Postgraduate Education Quality Improvement Plan of Shandong Province [No.SDYAL21150 to DING Gang]
文摘Periodontitis is a chronic infectious disease characterized by plaque biofilm as its main pathogenic factor;its clinical manifestations include gingival inflammation,alveolar bone resorption,attachment loss,and periodontal pocket formation,all of which eventually lead to tooth loss and seriously affect the quality of life of patients[1].This illness has a close relationship with blood sugar levels in patients with diabetes and is one of the most important complications of diabetes[2].Macrophages are an important part of host immune responses and exhibit powerful functions of identifying,phagocytizing,and removing bacteria;macrophage polarization is involved in the pathogenesis of periodontitis[3].
