Salt stress seriously restricts the growth and yield of potatoes.Plant cystatins are vital players in biotic stress and development,however,their roles in salt stress resistance remain elusive.Here,we report that StCY...Salt stress seriously restricts the growth and yield of potatoes.Plant cystatins are vital players in biotic stress and development,however,their roles in salt stress resistance remain elusive.Here,we report that StCYS1 positively regulates salt tolerance in potato plants.An in vitro biochemical test demonstrated that StCYS1 is a bona fide cystatin.Overexpression of StCYS1 in both Escherichia coli and potato plants significantly increased their resistance to high salinity.Further analysis revealed that the transgenic plants accumulated more proline and chlorophyll under salt stress conditions.Moreover,the transgenic plants displayed higher H2O2 scavenging capability and cell membrane integrity compared with wild-type potato.These results demonstrate that StCYS1 is closely correlated with salt stress and its overaccumulation can substantially enhance salt stress resistance.展开更多
An independent alternative calculation is performed for narrow near-threshold resonances in the e^(+)-He^(+) system using the stabilization method in the framework of hyperspherical coordinates (HSSM).A narrow resonan...An independent alternative calculation is performed for narrow near-threshold resonances in the e^(+)-He^(+) system using the stabilization method in the framework of hyperspherical coordinates (HSSM).A narrow resonance at E_(r )=-0.249995 with width T =1.9 × 10^(-5),associated with the He^(2+)-Ps(n =1) threshold is confirmed.The resonances around the energies -0.365 and -0.195,predicted by Bhatia and Drachman [Phys.Rev.A 42 (1990)5117] and confirmed by Ho [Phys.Rev.A 53 (1996)3165],do not appear in our calculations.展开更多
采用网络药理学方法和体外验证实验,探讨药食同源桔梗、百合配伍治疗肺炎的潜在分子机制。以生物利用度(OB)≥30%、类药性(DL)≥0.18为条件,在TCMSP数据库筛选桔梗和百合的活性成分,再通过TCMSP,DrugBank等数据库检索活性成分的预测靶点...采用网络药理学方法和体外验证实验,探讨药食同源桔梗、百合配伍治疗肺炎的潜在分子机制。以生物利用度(OB)≥30%、类药性(DL)≥0.18为条件,在TCMSP数据库筛选桔梗和百合的活性成分,再通过TCMSP,DrugBank等数据库检索活性成分的预测靶点,通过GeneCards和OMIM数据库获得肺炎的潜在靶点。将药物与疾病的靶点取交集获得共有靶点,运用STRING 11.0构建共有靶点PPI网络,拓扑分析获得核心靶点,再利用WebGestalt和Metascape对核心靶点进行GO和KEGG富集分析,然后,借助Cytoscape 3.7.1软件构建"成分-靶点-通路"网络,运用Discovery Studio 2016软件进行成分-靶点分子对接验证。最后,通过体外实验进行核心靶点和通路的初步验证。该研究共筛选出12个活性成分,基于数据挖掘方法,获得225个药物预测靶点和420个潜在疾病靶点,经拓扑分析获得14个核心靶点,包括TNF,MMP9,AKT1,IL4和IL2等,GO和KEGG富集结果表明桔梗-百合药对可能通过作用于TNF,IL-17等20条关键信号通路来调节炎症反应、细胞生长及代谢等过程,从而发挥抗肺炎作用。分子对接结果显示,12个活性成分与14个核心靶点均有较好的结合能力。体外实验结果显示,桔梗-百合药对核心成分可以通过调节TNF信号通路来抑制MMP9和TNF-α的表达。该研究证实了网络药理学预测结果的科学性与可靠性,并初步揭示了桔梗、百合配伍治疗肺炎的潜在分子机制,为系统地探索桔梗、百合配伍使用的作用机制提供了新颖的见解,同时对新药的研发与应用具有一定的参考价值。展开更多
A novel polyoxovanadate-based MOFs microsphere,[Ni(phen)V2O7]·H2O(phen=1,10-phenanthroline),constructed from 3-D discrete nano-sheets has been prepared and characterized by XRD,FT-IR,SEM and TEM.Electrochemical p...A novel polyoxovanadate-based MOFs microsphere,[Ni(phen)V2O7]·H2O(phen=1,10-phenanthroline),constructed from 3-D discrete nano-sheets has been prepared and characterized by XRD,FT-IR,SEM and TEM.Electrochemical properties as supercapacitor of the as-prepared sample,such as CV,EIS,GCD and the cycle life test have also been studied.The as-prepared MOF(V,Ni)showed a high specific capacitance of 178.09 F×g^(-1) at 1 A×g^(-1) as well as good cycling stability and coulombic efficiency.This work proved that the novel MOFs based on polyoxovanadate hybrid material may serve as a promising electrode material for high-performance supercapacitor.展开更多
基金We thank Li Guangcun(Institute of Vegetables and Flowers,Chinese Academy of Agricultural Sciences)for providing the potato(Solanum tuberosumL.)cultivar MDS.This work was supported by the National Natural Science Foundation of China(31901752)by a grant from the Potato Industry lnnovation Team for Modern Agricultural Industry Technology System,Shandong Province,China(SDAIT-10-011-11).
文摘Salt stress seriously restricts the growth and yield of potatoes.Plant cystatins are vital players in biotic stress and development,however,their roles in salt stress resistance remain elusive.Here,we report that StCYS1 positively regulates salt tolerance in potato plants.An in vitro biochemical test demonstrated that StCYS1 is a bona fide cystatin.Overexpression of StCYS1 in both Escherichia coli and potato plants significantly increased their resistance to high salinity.Further analysis revealed that the transgenic plants accumulated more proline and chlorophyll under salt stress conditions.Moreover,the transgenic plants displayed higher H2O2 scavenging capability and cell membrane integrity compared with wild-type potato.These results demonstrate that StCYS1 is closely correlated with salt stress and its overaccumulation can substantially enhance salt stress resistance.
基金Supported by the National Natural Science Foundation of China under Grant Nos 10927403 and 11004225.
文摘An independent alternative calculation is performed for narrow near-threshold resonances in the e^(+)-He^(+) system using the stabilization method in the framework of hyperspherical coordinates (HSSM).A narrow resonance at E_(r )=-0.249995 with width T =1.9 × 10^(-5),associated with the He^(2+)-Ps(n =1) threshold is confirmed.The resonances around the energies -0.365 and -0.195,predicted by Bhatia and Drachman [Phys.Rev.A 42 (1990)5117] and confirmed by Ho [Phys.Rev.A 53 (1996)3165],do not appear in our calculations.
文摘采用网络药理学方法和体外验证实验,探讨药食同源桔梗、百合配伍治疗肺炎的潜在分子机制。以生物利用度(OB)≥30%、类药性(DL)≥0.18为条件,在TCMSP数据库筛选桔梗和百合的活性成分,再通过TCMSP,DrugBank等数据库检索活性成分的预测靶点,通过GeneCards和OMIM数据库获得肺炎的潜在靶点。将药物与疾病的靶点取交集获得共有靶点,运用STRING 11.0构建共有靶点PPI网络,拓扑分析获得核心靶点,再利用WebGestalt和Metascape对核心靶点进行GO和KEGG富集分析,然后,借助Cytoscape 3.7.1软件构建"成分-靶点-通路"网络,运用Discovery Studio 2016软件进行成分-靶点分子对接验证。最后,通过体外实验进行核心靶点和通路的初步验证。该研究共筛选出12个活性成分,基于数据挖掘方法,获得225个药物预测靶点和420个潜在疾病靶点,经拓扑分析获得14个核心靶点,包括TNF,MMP9,AKT1,IL4和IL2等,GO和KEGG富集结果表明桔梗-百合药对可能通过作用于TNF,IL-17等20条关键信号通路来调节炎症反应、细胞生长及代谢等过程,从而发挥抗肺炎作用。分子对接结果显示,12个活性成分与14个核心靶点均有较好的结合能力。体外实验结果显示,桔梗-百合药对核心成分可以通过调节TNF信号通路来抑制MMP9和TNF-α的表达。该研究证实了网络药理学预测结果的科学性与可靠性,并初步揭示了桔梗、百合配伍治疗肺炎的潜在分子机制,为系统地探索桔梗、百合配伍使用的作用机制提供了新颖的见解,同时对新药的研发与应用具有一定的参考价值。
基金supported by the Natural Science Foundation of Fujian Province(No.2020J01803)Fujian Provincial Key Laboratory of Featured Materials in Biochemical Industry(FJKL_FBCM202004)the Fujian Provincial Key Laboratory of Ecotoxicological Effects and Control of New Pollutants(PY19001)。
文摘A novel polyoxovanadate-based MOFs microsphere,[Ni(phen)V2O7]·H2O(phen=1,10-phenanthroline),constructed from 3-D discrete nano-sheets has been prepared and characterized by XRD,FT-IR,SEM and TEM.Electrochemical properties as supercapacitor of the as-prepared sample,such as CV,EIS,GCD and the cycle life test have also been studied.The as-prepared MOF(V,Ni)showed a high specific capacitance of 178.09 F×g^(-1) at 1 A×g^(-1) as well as good cycling stability and coulombic efficiency.This work proved that the novel MOFs based on polyoxovanadate hybrid material may serve as a promising electrode material for high-performance supercapacitor.
