Long-span bridges are special structures that require advanced analysis techniques to examine their performance. This paper presents a procedure developed to model the Confederation Bridge using 3-D beam elements. The...Long-span bridges are special structures that require advanced analysis techniques to examine their performance. This paper presents a procedure developed to model the Confederation Bridge using 3-D beam elements. The model was validated using the data collected before the opening of the bridge to the public. The bridge was instrumented to conduct fullscale static and dynamic tests. The static tests were to measure the deflection of the bridge pier while the dynamic tests to measure the free vibrations of the pier due to a sudden release of the static load. Confederation Bridge is one of the longest reinforced concrete bridges in the world. It connects the province of Prince Edward Island and the province of New Brunswick in Canada. Due to its strategic location and vital role as a transportation link between these two provinces, it was designed using higher safety factors than those for typical highway bridges. After validating the present numerical model, a procedure was developed to evaluate the performance of similar bridges subjected to traffic and seismic loads. It is of interest to note that the foundation stiffness and the modulus of elasticity of the concrete have significant effects on the structural responses of the Confederation Bridge.展开更多
cis-Diamminedichloroplatinum(CDDP)is widely used for the treatment of various solid cancers.Here we reported that CDDP increased the expression and enzymatic activities of carboxylesterase 1(CES1)and carboxylesterase ...cis-Diamminedichloroplatinum(CDDP)is widely used for the treatment of various solid cancers.Here we reported that CDDP increased the expression and enzymatic activities of carboxylesterase 1(CES1)and carboxylesterase 2(CES2),along with the upregulation of pregnane X receptor(PXR)and the downregulation of differentiated embryonic chondrocyte-expressed gene 1(DEC1)in human hepatoma cells,primary mouse hepatocytes,mouse liver and intestine.The overexpression or knockdown of PXR alone upregulated or downregulated the CES1 and CES2 expression,respectively.The increases in CES1 and CES2 expression levels induced by CDDP abolished or enhanced by PXR knockdown or overexpression,implying that CDDP induces carboxylesterases through the activation of PXR.Likewise,the overexpression or knockdown of DEC1 alone significantly decreased or increased PXR and its targets.Moreover,the increases of PXR and its targets induced by CDDP were abolished or alleviated by the overexpression or knockdown of DEC1.The overexpression or knockdown of DEC1 affected the response of PXR to CDDP,but not vice versa,suggesting that CDDP increases carboxylesterases by upregulating PXR mediated by the decrease of DEC1.In addition,CDDP did not increase DEC1 mRNA degradation but suppressed DEC1 promoter reporter activity,indicating that it suppresses DEC1 transcriptionally.The combined use of CDDP and irinotecan had a synergistic effect on two cell lines,especially when CDDP was used first.展开更多
文摘Long-span bridges are special structures that require advanced analysis techniques to examine their performance. This paper presents a procedure developed to model the Confederation Bridge using 3-D beam elements. The model was validated using the data collected before the opening of the bridge to the public. The bridge was instrumented to conduct fullscale static and dynamic tests. The static tests were to measure the deflection of the bridge pier while the dynamic tests to measure the free vibrations of the pier due to a sudden release of the static load. Confederation Bridge is one of the longest reinforced concrete bridges in the world. It connects the province of Prince Edward Island and the province of New Brunswick in Canada. Due to its strategic location and vital role as a transportation link between these two provinces, it was designed using higher safety factors than those for typical highway bridges. After validating the present numerical model, a procedure was developed to evaluate the performance of similar bridges subjected to traffic and seismic loads. It is of interest to note that the foundation stiffness and the modulus of elasticity of the concrete have significant effects on the structural responses of the Confederation Bridge.
基金The work was supported by the National Natural Science Foundation of China(Grant Nos.82073934 and 81872937)to J.Y.
文摘cis-Diamminedichloroplatinum(CDDP)is widely used for the treatment of various solid cancers.Here we reported that CDDP increased the expression and enzymatic activities of carboxylesterase 1(CES1)and carboxylesterase 2(CES2),along with the upregulation of pregnane X receptor(PXR)and the downregulation of differentiated embryonic chondrocyte-expressed gene 1(DEC1)in human hepatoma cells,primary mouse hepatocytes,mouse liver and intestine.The overexpression or knockdown of PXR alone upregulated or downregulated the CES1 and CES2 expression,respectively.The increases in CES1 and CES2 expression levels induced by CDDP abolished or enhanced by PXR knockdown or overexpression,implying that CDDP induces carboxylesterases through the activation of PXR.Likewise,the overexpression or knockdown of DEC1 alone significantly decreased or increased PXR and its targets.Moreover,the increases of PXR and its targets induced by CDDP were abolished or alleviated by the overexpression or knockdown of DEC1.The overexpression or knockdown of DEC1 affected the response of PXR to CDDP,but not vice versa,suggesting that CDDP increases carboxylesterases by upregulating PXR mediated by the decrease of DEC1.In addition,CDDP did not increase DEC1 mRNA degradation but suppressed DEC1 promoter reporter activity,indicating that it suppresses DEC1 transcriptionally.The combined use of CDDP and irinotecan had a synergistic effect on two cell lines,especially when CDDP was used first.
