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Multispecies probiotic protects gut barrier function in experimental models 被引量:12
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作者 Mylene Nébot-Vivinus Cherryl Harkat +9 位作者 Hanene Bzioueche Christel Cartier Raffaella Plichon-Dainese lara moussa Helene Eutamene Dorsa Pishvaie Sophie Holowacz Christian Seyrig Thierry Piche Vassilia Theodorou 《World Journal of Gastroenterology》 SCIE CAS 2014年第22期6832-6843,共12页
AIM: To investigate the effect of the probiotic combination Lactibiane Tolerance<sup>&#x000ae;</sup> (LT) on epithelial barrier function in vitro and in vivo.
关键词 Irritable bowel syndrome Probiotic Intestinal epithelial barrier permeability HYPERSENSITIVITY
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Embedding MSCs in Si-HPMC hydrogel decreased MSC-directed host immune response and increased the regenerative potential of macrophages 被引量:1
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作者 Christelle Demarquay lara moussa +3 位作者 Gildas Réethorée Fabien Milliat Pierre Weiss Noëlle Mathieu 《Regenerative Biomaterials》 SCIE EI 2022年第1期490-500,共11页
Embedding mesenchymal stromal cells(MSCs)in biomaterial is a subject of increasing interest in the field of Regenerative Medicine.Speeding up the clinical use of MSCs is dependent on the use of non-syngeneic models in... Embedding mesenchymal stromal cells(MSCs)in biomaterial is a subject of increasing interest in the field of Regenerative Medicine.Speeding up the clinical use of MSCs is dependent on the use of non-syngeneic models in accordance with Good Manufacturing Practices(GMP)requirements and on costs.To this end,in this study,we analyzed the in vivo host immune response following local injection of silanized hydroxypropyl methylcellulose(Si-HPMC)-embedded human MSCs in a rat model developing colorectal damage induced by ionizing radiation.Plasma and lymphocytes from mesenteric lymph nodes were harvested in addition to colonic tissue.We set up tests,using flow cytometry and a live imaging system,to highlight the response to specific antibodies and measure the cytotoxicity of lymphocytes against injected MSCs.We demonstrated that Si-HPMC protects MSCs from specific antibodies production and from apoptosis by lymphocytes.We also observed that Si-HPMC does not modify innate immune response infiltrate in vivo,and that in vitro co-culture of Si-HPMC-embedded MSCs impacts macrophage inflammatory response depending on the microenvironment but,more importantly,increases the macrophage regenerative response through Wnt-family and VEGF gene expression.This study furthers our understanding of the mechanisms involved,with a view to improving the therapeutic benefits of biomaterial-assisted cell therapy by modulating the host immune response.The decrease in specific immune response against injected MSCs protected by Si-HPMC also opens up new possibilities for allogeneic clinical use. 展开更多
关键词 Si-HPMC hydrogel mesenchymal stromal cells host immune response regenerative medicine
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