Enhancing the tunneling magneto-Seebeck(TMS) ratio and uncovering its underlying mechanism are greatly demanded in spin caloritronics.The magnitude and sign of the TMS effect depend on the type of atom and the stackin...Enhancing the tunneling magneto-Seebeck(TMS) ratio and uncovering its underlying mechanism are greatly demanded in spin caloritronics.The magnitude and sign of the TMS effect depend on the type of atom and the stacking order of atoms at the interfaces.Herein,we demonstrate that TMS ratios can be effectively manipulated by altering heterogonous or homogeneous interface through decoration on the CoFeSi(001) surface inserted on the MgO insulating layers.The maximum TMS ratio of pure Co_(2)/O termination is 4565% at 800 K.Notably,the TMS ratio of the FeSi/O termination has two peak values,of which the maximum could reach up to-3290% at 650 K.By comparing two different atom arrangements at the interface,we reveal that the sign and symbol of the TMS ratio can be controlled by the temperature and different atomic configurations at the Co_(2)FeSi/MgO interface.Furthermore,the spin-Seebeck coefficient up to ~150 μV/K is also possible when we select suitable terminations and temperatures.These findings will provide useful insights into how to control the sign and symbol of the TMS ratio and accordingly stimulate the development field of magneto-thermoelectric power and spin caloritronic devices based on the magneto-Seebeck effect in Heusler-based metallic multilayers.展开更多
BACKGROUND Gastrointestinal neoplasm(GN)significantly impact the global cancer burden and mortality,necessitating early detection and treatment.Understanding the evolution and current state of research in this field i...BACKGROUND Gastrointestinal neoplasm(GN)significantly impact the global cancer burden and mortality,necessitating early detection and treatment.Understanding the evolution and current state of research in this field is vital.AIM To conducts a comprehensive bibliometric analysis of publications from 1984 to 2022 to elucidate the trends and hotspots in the GN risk assessment research,focusing on key contributors,institutions,and thematic evolution.METHODS This study conducted a bibliometric analysis of data from the Web of Science Core Collection database using the"bibliometrix"R package,VOSviewer,and CiteSpace.The analysis focused on the distribution of publications,contributions by institutions and countries,and trends in keywords.The methods included data synthesis,network analysis,and visualization of international collaboration networks.RESULTS This analysis of 1371 articles on GN risk assessment revealed a notable evolution in terms of research focus and collaboration.It highlights the United States'critical role in advancing this field,with significant contributions from institutions such as Brigham and Women's Hospital and the National Cancer Institute.The last five years,substantial advancements have been made,representing nearly 45%of the examined literature.Publication rates have dramatically increased,from 20 articles in 2002 to 112 in 2022,reflecting intensified research efforts.This study underscores a growing trend toward interdisciplinary and international collaboration,with the Journal of Clinical Oncology standing out as a key publication outlet.This shift toward more comprehensive and collaborative research methods marks a significant step in addressing GN risks.CONCLUSION This study underscores advancements in GN risk assessment through genetic analyses and machine learning and reveals significant geographical disparities in research emphasis.This calls for enhanced global collaboration and integration of artificial intelligence to improve cancer prevention and treatment accuracy,ultimately enhancing worldwide patient care.展开更多
Catgut implantation at acupoints has been shown to alleviate spasticity after stroke in rats.However,the underlying mechanisms are poorly understood.In this study,we used the rat middle cerebral artery occlusion model...Catgut implantation at acupoints has been shown to alleviate spasticity after stroke in rats.However,the underlying mechanisms are poorly understood.In this study,we used the rat middle cerebral artery occlusion model of stroke.Three days after surgery,absorbable surgical catgut sutures were implanted at Dazhui（GV14）,Jizhong（GV6）,Houhui,Guanyuan（CV4）and Zhongwan（CV12）.The Zea Longa score was used to assess neurological function.The Modified Ashworth Scale was used to evaluate muscle tension.The 2,3,5-triphenyl-tetrazolium chloride assay was used to measure infarct volume.Immunohistochemical staining was performed for glutamate aspartate transporter（GLAST）and glial glutamate transporter-1（GLT-1）expression.Western blot assay was used to analyze the expression of GLAST and GLT-1.Reverse transcription and polymerase chain reaction were carried out to assess the expression of GLAST and GLT-1m RNAs.After catgut implantation at the acupoints,neurological function was substantially improved,muscle tension was decreased,and infarct volume was reduced in rats with spasticity after stroke.Furthermore,the expression of GLAST and GLT-1 m RNAs was increased on the injured（left）side.Our findings demonstrate that catgut implantation at acupoints alleviates spasticity after stroke,likely by increasing the expression of GLAST and GLT-1.展开更多
·AIM: To determine the prevalence of asthenopia and identify any associated risk factors in the college students in Xi’an, China. · METHODS: From April to September 2012, 1 500 students from five universiti...·AIM: To determine the prevalence of asthenopia and identify any associated risk factors in the college students in Xi’an, China. · METHODS: From April to September 2012, 1 500 students from five universities in Xi’an were selected according to a multi -stage stratified cluster sampling method. Data on demographic features, lifestyle or dietary habits, health status, living environment conditions, sleep and mental status, and asthenopia symptoms were collected through a self -administered validated questionnaire. Univariate logistic regression and multivariate logistic regression analysis modified by the factor analysis were performed to evaluate risk factors for asthenopia. ·RESULTS: Fifty-seven percent of the college students complained of asthenopia. Statistically significant risk factors for asthenopia in the univariate analysis included 13 variables. Multivariate analysis revealed a significant relationship between the use of computer and asthenopia (OR 1.21, 95% CI: 1.09 to 1.35). Good sleep and mental status (OR 0.86, 95% CI: 0.76 to 0.97), good living environment conditions (OR 0.67, 95% CI: 0.60 to 0.76), and high intake of green leafy vegetables (OR 0.89, 95% CI: 0.80 to 0.98) were found to be strong predictors of decreasing the occurrence of asthenopia complaints. ·CONCLUSION: Asthenopia symptom appeares to be common among college students; and it is strongly associated with computer use, psychosocial state, environment conditions and dietary habits, suggesting that additional studies are warranted to verify these risk factors and establish prevention guidelines, especially for college students. ·展开更多
AIM： To investigate the associations between fruit and vegetable consumption and risk of asthenopia among Chinese college students. METHODS： A total of 1022 students were selected from five universities by a multi-s...AIM： To investigate the associations between fruit and vegetable consumption and risk of asthenopia among Chinese college students. METHODS： A total of 1022 students were selected from five universities by a multi-stage stratified cluster sampling method. They were surveyed via a self-administered questionnaire including socio-demographic features, dietary and lifestyle habits, eye-related symptoms, eye care habits and history of diseases. Ascertainment of asthenopia was based on participants＇ subjectively reported symptoms. The associations between fruit and vegetable intake with asthenopia risk were assessed using multivariate logistic regression analysis. RESULTS： There were no significant associations between total fruit and vegetable, total vegetable, or fruit and the risk of asthenopia. Higher intake of dark-green leafy vegetable was likely to be inversely associated with asthenopia risk [odd ratio （OR）： 0.60; 95%CI： 0.37-0.97; Ptrend=0.21] after controlling for nondietary and dietary risk factors. Stratified analysis showed that the inverse association between dark-green leafy vegetable intake and asthenopia risk was limited to participants with suboptimal eyesight （OR： 0.45; 95%CI： 0.25-0.82; Ptrend=0.05）, wearing glasses （OR： 0.35; 95%CI： 0.17-0.72; Ptrend=0.03） or using computer ≥3h/d （OR： 0.48; 95%CI： 0.25-0.93; Ptrend=0.08）. CONCLUSION： A higher consumption of dark-green leafy vegetable is associated with a lower asthenopia risk among college students with suboptimal eyesight and poor eye care habits.展开更多
AIM:To evaluate the predictive value of islet autoantibodies for the diagnosis of autoimmune uveitis(AU),as well as to characterize the association bet ween islet autoantibodies and AU.METHODS:Totally 97 patients with...AIM:To evaluate the predictive value of islet autoantibodies for the diagnosis of autoimmune uveitis(AU),as well as to characterize the association bet ween islet autoantibodies and AU.METHODS:Totally 97 patients with AU and 100 healthy persons without any autoimmune diseases as the control group were recruited.Multiple serum islet autoantibodies were measured using commercial enzyme-linked immunosorbent assay kits(ELISA).A supplementary questionnaire was used to complement the subject's demographics and clinical features.The level of glucose concentrations and white blood cells were measured.Conditional logistic regression was performed to estimate odds ratios(ORs),and 95%confidence intervals(CIs)of AU according to islet autoantibodies and to evaluate the predictive value of islet autoantibodies for AU diagnosis.Autoantibodies subgroups and other variables were included into analysis.RESULTS:In AU patients,the prevalence of detecting at least one of the autoantibodies was 31.9%(31/97).The most frequent autoantibody was ZnT8A(30.9%),followed by GADA(11.3%),IA-2A(4.1%),ICA(2.1%)and IAA(2.1%).Islet autoantibodies were found to be correlated positively with AU diagnosis[OR(95%CI):13.86(3.28,58.50),P<0.001].Moreover,Zn-T8A was remarkably correlated with AU diagnosis[OR(95%CI):6.13(1.96,19.17),P<0.001],In contrast,neither GADA nor other islet antibodies(IA-2A,ICA and IAA)showed any association with AU risk under an additive model.CONCLUSION:The prevalence of islet antibodies,especially ZnT8A,in patients with AU is higher.Islet antibodies as well as novel biomarkers should be included in routine evaluation at AU and is a valuable biological marker to classify newly-diagnosed uveitis.展开更多
To the Editor:In the last decade,neoadjuvant chemotherapy(NAC)has become a well-accepted treatment option for breast cancer,although few detailed description of NAC in China has yet been reported.[1]A previous study f...To the Editor:In the last decade,neoadjuvant chemotherapy(NAC)has become a well-accepted treatment option for breast cancer,although few detailed description of NAC in China has yet been reported.[1]A previous study found that among patients with clinically node-negative(cN0)breast cancer,97.7%(432/442)with breast pathologic complete response(bpCR)had ypN0(absence of metastases in the axillary lymph nodes);and 71.6%(882/1232)without bpCR achieved ypN0(P<0.001).As for human epidermal growth factor receptor 2(HER2)positive or triple-negative breast cancer(TNBC)that achieved bpCR.展开更多
AIM: To summarize quantitatively the prospective association between physical activity and age-related cataract(ARC) risk.METHODS: Pub Med, Embase, Web of Science, and Cochrane Library were systematically searched for...AIM: To summarize quantitatively the prospective association between physical activity and age-related cataract(ARC) risk.METHODS: Pub Med, Embase, Web of Science, and Cochrane Library were systematically searched for all relevant follow up studies until July 2019. Multivariableadjusted relative risks(RRs) and corresponding 95% confidence intervals(CIs) from individual studies were used to calculate the overall summary estimates. The doseresponse relationship was assessed using generalized least-squares trend estimation.RESULTS: Six prospective cohort studies, involving 19 173 cases in 6.2-12.1 y follow up of 171 620 participants, were included in the analysis. Increased physical activity was significantly associated with reduced risk of ARC by 10%(RR: 0.90;95%CI: 0.81, 0.99, P=0.04). Stratified analysis by assessment method for physical activity suggest that studies using metabolic equivalent(MET) per day tended to report a slightly stronger association with ARC(RR: 0.85;95%CI: 0.81, 0.90, P<0.001) than studies which assessed activity by weekly activity(RR: 0.96;95%CI: 0.89, 1.03, P=0.24). Dose-response analysis indicated that the risk of ARC decreased by 2%(RR: 0.98;95%CI: 0.98, 0.99, P<0.001) for every 6 METs per day increase in activity.CONCLUSION: The findings from this Meta-analysis provide additional evidence that increased physical activity is inversely associated with ARC risk dose-responsively.展开更多
To the Editor:Allogeneic hematopoietic stem cell transplantation(allo-HSCT)is an important treatment for hematologic disease.High-intensity preconditioning in allo-HSCT patients significantly increases the risk of sev...To the Editor:Allogeneic hematopoietic stem cell transplantation(allo-HSCT)is an important treatment for hematologic disease.High-intensity preconditioning in allo-HSCT patients significantly increases the risk of severe infection,which is an important cause of early transplant-related death.展开更多
Background：Salvianolic acid B （Sal B） is a bioactive water-soluble compound of Salviae miltiorrhizae,a traditional herbal medicine that has been used clinically tor the treatment of cardiovascular diseases.This stu...Background：Salvianolic acid B （Sal B） is a bioactive water-soluble compound of Salviae miltiorrhizae,a traditional herbal medicine that has been used clinically tor the treatment of cardiovascular diseases.This study sought to evaluate the effect of Sal B on matrix metalloproteinase-9 （MMP-9） and on the underlying mechanisms in tumor necrosis factor-α （TNF-α）-activated human coronary artery endothelial cells （HCAECs）,a cell model of Kawasaki disease.Methods：HCAECs were pretreated with 1 l0 μmol/L of Sal B,and then stimulated by TNF-α at different time points.The protein expression and activity of MMP-9 were determined by Western blot assay and gelatin zymogram assay,respectively.Nuclear factor-κB （NF-κB） activation was detected with immunofluorescence,electrophoretic mobility shift assay,and Western blot assay.Protein expression levels of mitogen-activated protein kinase （c-Jun N-terminal kinase [JNK],extra-cellular signal-regulated kinase [ERK],and p38） were determined by Western blot assay.Results：After HCAECs were exposed to TNF-α,1-10 μtmol/L Sal B significantly inhibited TNF-α-induced MMP-9 expression and activity.Furthermore,Sal B significantly decreased IκBα phosphorylation and p65 nuclear translocation in HCAECs stimulated with TNF-α for 30 min.In addition,Sal B decreased the phosphorylation of JNK and ERK1/2 proteins in cells treated with TNF-α for 10 min.Conclusions：The data suggested that Sal B suppressed TNF-α-induced MMP-9 expression and activity by blocking the activation of NF-κB,JNK,and ERK1/2 signaling pathways.展开更多
Background Juvenile idiopathic arthritis(JIA)is the most common rheumatic disease in childhood driven by aberrant pathways of T-cell activation.T helper 17(Th17)/regulatory T cell(Treg)imbalance plays critical roles i...Background Juvenile idiopathic arthritis(JIA)is the most common rheumatic disease in childhood driven by aberrant pathways of T-cell activation.T helper 17(Th17)/regulatory T cell(Treg)imbalance plays critical roles in the pathogenesis of arthritis.MicroRNA-125b(miR-125b)was upregulated after the activation of the initial CD4^+T cells,and could regulate the differentiation of CD4^+T cells.However,the effects of miR-125b on Th17/Treg imbalance and differentiation of Th 17/Treg cells remain unknown.Methods In this study,we evaluated the expression of miR-125b in the peripheral blood mononuclear cells(PBMCs)of children with JIA,and the relationship of miR-125b with Th17/Treg imbalance.Then,we used lentivirus vector-mediated overexpression technology to investigate the regulatory function of miR-125b in CD4^+T cells or dendritic cell/CD4^+T co-culture system.Results Decreased miR-125b expression in PBMCs and CD4^+T cells of JIA patients was negatively correlated with the ratio of Th17/Treg cells.It also correlated negatively with retinoic acid receptor-related orphan receptorγt but positively with Forkhead box protein 3 at transcriptional levels.Furthermore,we found that miR-125b overexpression inhibited Th17 cell differentiation,whereas facilitated the differentiation of Treg cells.MiR-125b upregulation led to the decrease of Th 17-secreting cytokines but the increase of the Treg-secreting cytokines.Conclusions Our results demonstrate that miR-125b participated in regulating Thl7/Treg cell differentiation and imbalance in JIA patients.These findings provide novel insight into the critical role of miR-125b in the Th17/Treg imbalance of JIA,and raise the distinct possibility that miR-125b may prove to be a potential therapeutic target for JIA.展开更多
Evaluating anthropogenic impacts on regional vegetation changes during historical time is not only important for a better understanding of the Anthropocene but also valuable in improving the vegetation-climate models....Evaluating anthropogenic impacts on regional vegetation changes during historical time is not only important for a better understanding of the Anthropocene but also valuable in improving the vegetation-climate models.In this study,we analyzed stable isotopes(δ^18O,δ^13C)and trace elements(Mg/Ca,Sr/Ca)of a stalagmite from Huangchao Cave in central Shandong,northern China.230Th and AMS14C dating results indicate the stalagmite deposited during 174BC and AD1810,with a hiatus between AD638 and 1102.Broad similarities of theδ^18O and trace elements in the stalagmite suggest they are reliable precipitation indexes.Theδ^13C of the stalagmite,a proxy of vegetation change,was generally consistent with local precipitation and temperature variations on a centennial-scale before the 15th century.It typically varied from–9.6‰to–6.3‰,indicating climate controlled C3 type vegetation during this period.However,a persistent and marked increasing trend in theδ^13C record was observed since the 15th century,resulting inδ^13C values from–7.7‰to–1.6‰in the next four centuries.This unprecedentedδ^13C change caused by vegetation deterioration cannot be explained by climate change but is fairly consistent with the dramatically increasing population and farmland in Shandong.We suggest that the increasing deforestation and reclamation in central Shandong began to affect vegetation in the mountain region of central Shandong since the 15th century and severely destroyed or even cleared the forest during the 16th–18th century.展开更多
With the accelerated development of electronic devices,micro-supercapacitors(MSCs),as energy storage devices that can charge and discharge quickly,have attracted considerable attention.To improve the rate capability o...With the accelerated development of electronic devices,micro-supercapacitors(MSCs),as energy storage devices that can charge and discharge quickly,have attracted considerable attention.To improve the rate capability of MSCs with consideration of the energy density remains a challenge.We demonstrated a facile method for the preparation of thin films through bottom-up femtosecond pulsed laser deposition.The femtosecond laser irradiated the polyimide film through a transparent substrate to uniformly sputter the electrode material onto the lower surface of the substrate.We successfully deposited porous amorphous carbon,graphene,and carbon quantum dots with controllable properties by temporally shaping the femtosecond laser.The resulting MSC exhibited an ultrahigh frequency response and good performance at scan rates up to 10,000 V s^(-1).The characteristic frequency f_(0) of the MSC was as high as 42,000 Hz,and the relaxation time constant τ_(0) was 0.0238 ms.The MSC reached an impedance phase angle of -82.6° at a frequency of 120 Hz,an ultrahigh power density of more than 30 kW cm^(-3),and an energy density of 0.068 W h cm-3.This method provides a novel perspective for the preparation of ultrahigh frequency filters for future miniaturized portable electronic devices.展开更多
Zika virus(ZIKV) is associated with severe birth defects and Guillain-Barre′ syndrome and no approved vaccines or specific therapies to combat ZIKV infection are currently available. To accelerate anti-ZIKV therapeut...Zika virus(ZIKV) is associated with severe birth defects and Guillain-Barre′ syndrome and no approved vaccines or specific therapies to combat ZIKV infection are currently available. To accelerate anti-ZIKV therapeutics research, we developed a stable ZIKV GFP-reporter virus system with considerably improved GFP visibility and stability. In this system a BHK-21 cell line expressing DC-SIGNR was established to facilitate the proliferation of GFP-reporter ZIKV. Using this reporter virus system, we established a high-throughput screening assay and screened a selected plant-sourced compounds library for their ability to block ZIKV infection. More than 31 out of 974 tested compounds effectively decreased ZIKV reporter infection. Four selected compounds, homoharringtonine(HHT), bruceine D(BD), dihydroartemisinin(DHA) and digitonin(DGT), were further validated to inhibit wild-type ZIKV infection in cells of BHK-21 and human cell line A549.The FDA-approved chronic myeloid leukemia treatment drug HHT and BD were identified as broad-spectrum flavivirus inhibitors. DHA, another FDA-approved antimalarial drug effectively inhibited ZIKV infection in BHK-21 cells. HHT, BD and DHA inhibited ZIKV infection at a post-entry stage. Digitonin was found to have inhibitory activity in the early stage of viral infection. Our research provides an efficient high-throughput screening assay for ZIKV inhibitors. The active compounds identified in this study represent potential therapies for the treatment of ZIKV infection.展开更多
The treatment and healing of infected skin lesions is one of the major challenges in surgery.To solve this problem,collagen I(Col-I)and the antibacterial agent hydroxypropyltrimethyl ammonium chloride chitosan(HACC)we...The treatment and healing of infected skin lesions is one of the major challenges in surgery.To solve this problem,collagen I(Col-I)and the antibacterial agent hydroxypropyltrimethyl ammonium chloride chitosan(HACC)were composited into the bacterial cellulose(BC)three-dimensional network structure by a novel membrane-liquid interface(MLI)culture,and a Col-I/HACC/BC(CHBC)multifunctional dressing was designed.The water absorption rate and water vapor transmission rate of the obtained CHBC dressing were 35.78±2.45 g/g and 3084±56 g m^(-2)⋅day^(-1),respectively.The water retention of the CHBC dressing was significantly improved compared with the BC caused by the introduced Col-I and HACC.In vitro results indicated that the combined advantages of HACC and Col-I confer on CHBC dressings not only have outstanding antibacterial properties against Staphylococcus aureus(S.aureus)compared with BC and CBC,but also exhibit better cytocompatibility than BC and HBC to promote the proliferation and spread of NIH3T3 cells and HUVECs.Most importantly,the results of in vivo animal tests demonstrated that the CHBC dressings fully promoted wound healing for 8 days and exhibited shorter healing times,especially in the case of wound infection.Excellent skin regeneration effects and higher expression levels of collagen during infection were also shown in the CHBC group.We believe that CHBC composites with favorable multifunctionality have potential applications as wound dressings to treat infected wounds.展开更多
Radiotherapy is one of the main cancer treatments,but it may damage normal tissue and cause various side effects.At present,radioprotective agents used in clinics have side effects such as nausea,vomiting,diarrhea and...Radiotherapy is one of the main cancer treatments,but it may damage normal tissue and cause various side effects.At present,radioprotective agents used in clinics have side effects such as nausea,vomiting,diarrhea and hypotension,which limit their clinical application.It has been found that exosomes play an indispensable role in radiation injury.Exosomes are lipid bilayer vesicles that carry various bioactive substances,such as proteins,lipids and microRNA(miRNA),that play a key role in cell-to-cell communication and affect tissue injury and repair.In addition,studies have shown that radiation can increase the uptake of exosomes in cells and affect the composition and secretion of exosomes.Here,we review the existing studies and discuss the effects of radiation on exosomes and the role of exosomes in radiation injury,aiming to provide new insights for the treatment of radiation injury.展开更多
基金supported by the National Natural Science Foundation of China (Grant No. 12104458)Foshan (Southern China) Institute for New Materials (Grant No. 2021AYF25021)。
文摘Enhancing the tunneling magneto-Seebeck(TMS) ratio and uncovering its underlying mechanism are greatly demanded in spin caloritronics.The magnitude and sign of the TMS effect depend on the type of atom and the stacking order of atoms at the interfaces.Herein,we demonstrate that TMS ratios can be effectively manipulated by altering heterogonous or homogeneous interface through decoration on the CoFeSi(001) surface inserted on the MgO insulating layers.The maximum TMS ratio of pure Co_(2)/O termination is 4565% at 800 K.Notably,the TMS ratio of the FeSi/O termination has two peak values,of which the maximum could reach up to-3290% at 650 K.By comparing two different atom arrangements at the interface,we reveal that the sign and symbol of the TMS ratio can be controlled by the temperature and different atomic configurations at the Co_(2)FeSi/MgO interface.Furthermore,the spin-Seebeck coefficient up to ~150 μV/K is also possible when we select suitable terminations and temperatures.These findings will provide useful insights into how to control the sign and symbol of the TMS ratio and accordingly stimulate the development field of magneto-thermoelectric power and spin caloritronic devices based on the magneto-Seebeck effect in Heusler-based metallic multilayers.
基金Supported by National Natural Science Foundation of China,No.72104183Shanghai Municipal Health Commission Project,No.20234Y0057+4 种基金Shanghai Sailing Program,No.20YF1444900Shanghai Hospital Association Project,No.X2022142Projects of the Committee of Shanghai Science and Technology,No.20Y11913700Guangdong Association of Clinical Trials(GACT)/Chinese Thoracic Oncology Group(CTONG)and Guangdong Provincial Key Lab of Translational Medicine in Lung Cancer,No.2017B030314120Beijing CSCO(Sisco)Clinical Oncology Research Grant,No.Y-HS202101-0205.
文摘BACKGROUND Gastrointestinal neoplasm(GN)significantly impact the global cancer burden and mortality,necessitating early detection and treatment.Understanding the evolution and current state of research in this field is vital.AIM To conducts a comprehensive bibliometric analysis of publications from 1984 to 2022 to elucidate the trends and hotspots in the GN risk assessment research,focusing on key contributors,institutions,and thematic evolution.METHODS This study conducted a bibliometric analysis of data from the Web of Science Core Collection database using the"bibliometrix"R package,VOSviewer,and CiteSpace.The analysis focused on the distribution of publications,contributions by institutions and countries,and trends in keywords.The methods included data synthesis,network analysis,and visualization of international collaboration networks.RESULTS This analysis of 1371 articles on GN risk assessment revealed a notable evolution in terms of research focus and collaboration.It highlights the United States'critical role in advancing this field,with significant contributions from institutions such as Brigham and Women's Hospital and the National Cancer Institute.The last five years,substantial advancements have been made,representing nearly 45%of the examined literature.Publication rates have dramatically increased,from 20 articles in 2002 to 112 in 2022,reflecting intensified research efforts.This study underscores a growing trend toward interdisciplinary and international collaboration,with the Journal of Clinical Oncology standing out as a key publication outlet.This shift toward more comprehensive and collaborative research methods marks a significant step in addressing GN risks.CONCLUSION This study underscores advancements in GN risk assessment through genetic analyses and machine learning and reveals significant geographical disparities in research emphasis.This calls for enhanced global collaboration and integration of artificial intelligence to improve cancer prevention and treatment accuracy,ultimately enhancing worldwide patient care.
基金supported by the National Natural Science Foundation of China,No.14202225,&.81574042the Traditional Chinese Medicine Leading Talent Funding Projects of Henan Province of China,No.2000202a grant from the Special Research Project on the Construction of the National Traditional Chinese Medicine Clinical Research Base of the State Administration of Traditional Chinese Medicine of China,No.JDZX2015314
文摘Catgut implantation at acupoints has been shown to alleviate spasticity after stroke in rats.However,the underlying mechanisms are poorly understood.In this study,we used the rat middle cerebral artery occlusion model of stroke.Three days after surgery,absorbable surgical catgut sutures were implanted at Dazhui（GV14）,Jizhong（GV6）,Houhui,Guanyuan（CV4）and Zhongwan（CV12）.The Zea Longa score was used to assess neurological function.The Modified Ashworth Scale was used to evaluate muscle tension.The 2,3,5-triphenyl-tetrazolium chloride assay was used to measure infarct volume.Immunohistochemical staining was performed for glutamate aspartate transporter（GLAST）and glial glutamate transporter-1（GLT-1）expression.Western blot assay was used to analyze the expression of GLAST and GLT-1.Reverse transcription and polymerase chain reaction were carried out to assess the expression of GLAST and GLT-1m RNAs.After catgut implantation at the acupoints,neurological function was substantially improved,muscle tension was decreased,and infarct volume was reduced in rats with spasticity after stroke.Furthermore,the expression of GLAST and GLT-1 m RNAs was increased on the injured（left）side.Our findings demonstrate that catgut implantation at acupoints alleviates spasticity after stroke,likely by increasing the expression of GLAST and GLT-1.
基金Fundamental Research Funds for the Central Universities of China(No.xjj2012052)
文摘·AIM: To determine the prevalence of asthenopia and identify any associated risk factors in the college students in Xi’an, China. · METHODS: From April to September 2012, 1 500 students from five universities in Xi’an were selected according to a multi -stage stratified cluster sampling method. Data on demographic features, lifestyle or dietary habits, health status, living environment conditions, sleep and mental status, and asthenopia symptoms were collected through a self -administered validated questionnaire. Univariate logistic regression and multivariate logistic regression analysis modified by the factor analysis were performed to evaluate risk factors for asthenopia. ·RESULTS: Fifty-seven percent of the college students complained of asthenopia. Statistically significant risk factors for asthenopia in the univariate analysis included 13 variables. Multivariate analysis revealed a significant relationship between the use of computer and asthenopia (OR 1.21, 95% CI: 1.09 to 1.35). Good sleep and mental status (OR 0.86, 95% CI: 0.76 to 0.97), good living environment conditions (OR 0.67, 95% CI: 0.60 to 0.76), and high intake of green leafy vegetables (OR 0.89, 95% CI: 0.80 to 0.98) were found to be strong predictors of decreasing the occurrence of asthenopia complaints. ·CONCLUSION: Asthenopia symptom appeares to be common among college students; and it is strongly associated with computer use, psychosocial state, environment conditions and dietary habits, suggesting that additional studies are warranted to verify these risk factors and establish prevention guidelines, especially for college students. ·
基金Supported by the National Natural Science Foundation of China(No.81202198)the China Postdoctoral Science Special Foundation(No.2015T81036)+2 种基金the China Postdoctoral Science Foundation Funded Project(No.2014M560790)the Fundamental Research Funds for the Central Universities(No.qngz2016004)the Newstar Plan of Science and Technology of Shaanxi Province(No.2015KJXX-07)
文摘AIM： To investigate the associations between fruit and vegetable consumption and risk of asthenopia among Chinese college students. METHODS： A total of 1022 students were selected from five universities by a multi-stage stratified cluster sampling method. They were surveyed via a self-administered questionnaire including socio-demographic features, dietary and lifestyle habits, eye-related symptoms, eye care habits and history of diseases. Ascertainment of asthenopia was based on participants＇ subjectively reported symptoms. The associations between fruit and vegetable intake with asthenopia risk were assessed using multivariate logistic regression analysis. RESULTS： There were no significant associations between total fruit and vegetable, total vegetable, or fruit and the risk of asthenopia. Higher intake of dark-green leafy vegetable was likely to be inversely associated with asthenopia risk [odd ratio （OR）： 0.60; 95%CI： 0.37-0.97; Ptrend=0.21] after controlling for nondietary and dietary risk factors. Stratified analysis showed that the inverse association between dark-green leafy vegetable intake and asthenopia risk was limited to participants with suboptimal eyesight （OR： 0.45; 95%CI： 0.25-0.82; Ptrend=0.05）, wearing glasses （OR： 0.35; 95%CI： 0.17-0.72; Ptrend=0.03） or using computer ≥3h/d （OR： 0.48; 95%CI： 0.25-0.93; Ptrend=0.08）. CONCLUSION： A higher consumption of dark-green leafy vegetable is associated with a lower asthenopia risk among college students with suboptimal eyesight and poor eye care habits.
基金Supported by the Science Foundation of Shaanxi Province of China(No.2017SF-265)the Science Foundation of Xi’an of China[No.2017121SF/YX015(2)].
文摘AIM:To evaluate the predictive value of islet autoantibodies for the diagnosis of autoimmune uveitis(AU),as well as to characterize the association bet ween islet autoantibodies and AU.METHODS:Totally 97 patients with AU and 100 healthy persons without any autoimmune diseases as the control group were recruited.Multiple serum islet autoantibodies were measured using commercial enzyme-linked immunosorbent assay kits(ELISA).A supplementary questionnaire was used to complement the subject's demographics and clinical features.The level of glucose concentrations and white blood cells were measured.Conditional logistic regression was performed to estimate odds ratios(ORs),and 95%confidence intervals(CIs)of AU according to islet autoantibodies and to evaluate the predictive value of islet autoantibodies for AU diagnosis.Autoantibodies subgroups and other variables were included into analysis.RESULTS:In AU patients,the prevalence of detecting at least one of the autoantibodies was 31.9%(31/97).The most frequent autoantibody was ZnT8A(30.9%),followed by GADA(11.3%),IA-2A(4.1%),ICA(2.1%)and IAA(2.1%).Islet autoantibodies were found to be correlated positively with AU diagnosis[OR(95%CI):13.86(3.28,58.50),P<0.001].Moreover,Zn-T8A was remarkably correlated with AU diagnosis[OR(95%CI):6.13(1.96,19.17),P<0.001],In contrast,neither GADA nor other islet antibodies(IA-2A,ICA and IAA)showed any association with AU risk under an additive model.CONCLUSION:The prevalence of islet antibodies,especially ZnT8A,in patients with AU is higher.Islet antibodies as well as novel biomarkers should be included in routine evaluation at AU and is a valuable biological marker to classify newly-diagnosed uveitis.
基金Beijing Medical Reward Foundation(No.YXJL-2016-0040-0012)
文摘To the Editor:In the last decade,neoadjuvant chemotherapy(NAC)has become a well-accepted treatment option for breast cancer,although few detailed description of NAC in China has yet been reported.[1]A previous study found that among patients with clinically node-negative(cN0)breast cancer,97.7%(432/442)with breast pathologic complete response(bpCR)had ypN0(absence of metastases in the axillary lymph nodes);and 71.6%(882/1232)without bpCR achieved ypN0(P<0.001).As for human epidermal growth factor receptor 2(HER2)positive or triple-negative breast cancer(TNBC)that achieved bpCR.
基金Supported by the National Natural Science Foundation of China(No.81473059)the Natural Science Foundation of Shaanxi Province of China(No.2017JM8041)the China Postdoctoral Science Special Foundation(No.2015T81036)。
文摘AIM: To summarize quantitatively the prospective association between physical activity and age-related cataract(ARC) risk.METHODS: Pub Med, Embase, Web of Science, and Cochrane Library were systematically searched for all relevant follow up studies until July 2019. Multivariableadjusted relative risks(RRs) and corresponding 95% confidence intervals(CIs) from individual studies were used to calculate the overall summary estimates. The doseresponse relationship was assessed using generalized least-squares trend estimation.RESULTS: Six prospective cohort studies, involving 19 173 cases in 6.2-12.1 y follow up of 171 620 participants, were included in the analysis. Increased physical activity was significantly associated with reduced risk of ARC by 10%(RR: 0.90;95%CI: 0.81, 0.99, P=0.04). Stratified analysis by assessment method for physical activity suggest that studies using metabolic equivalent(MET) per day tended to report a slightly stronger association with ARC(RR: 0.85;95%CI: 0.81, 0.90, P<0.001) than studies which assessed activity by weekly activity(RR: 0.96;95%CI: 0.89, 1.03, P=0.24). Dose-response analysis indicated that the risk of ARC decreased by 2%(RR: 0.98;95%CI: 0.98, 0.99, P<0.001) for every 6 METs per day increase in activity.CONCLUSION: The findings from this Meta-analysis provide additional evidence that increased physical activity is inversely associated with ARC risk dose-responsively.
基金Intramural Research Project(Nos.AWS17J007 and 2020XQN13)
文摘To the Editor:Allogeneic hematopoietic stem cell transplantation(allo-HSCT)is an important treatment for hematologic disease.High-intensity preconditioning in allo-HSCT patients significantly increases the risk of severe infection,which is an important cause of early transplant-related death.
基金Acknowledgments We thank Medjaden and Editage for its linguistic assistance during the preparation of this manuscript. Financial support and sponsorship This study was supported by the grants from National Natural Science Foundation of China （No. 81274109, 30973238）, Key Research Project of Beijing Natural Science Foundation （B）/Beijing Education Committee （No. KZ201010025024）, and Project for Science and Technology Innovation, Beijing Education Committee （No. PXM2011 014226 07 000085）.
文摘Background：Salvianolic acid B （Sal B） is a bioactive water-soluble compound of Salviae miltiorrhizae,a traditional herbal medicine that has been used clinically tor the treatment of cardiovascular diseases.This study sought to evaluate the effect of Sal B on matrix metalloproteinase-9 （MMP-9） and on the underlying mechanisms in tumor necrosis factor-α （TNF-α）-activated human coronary artery endothelial cells （HCAECs）,a cell model of Kawasaki disease.Methods：HCAECs were pretreated with 1 l0 μmol/L of Sal B,and then stimulated by TNF-α at different time points.The protein expression and activity of MMP-9 were determined by Western blot assay and gelatin zymogram assay,respectively.Nuclear factor-κB （NF-κB） activation was detected with immunofluorescence,electrophoretic mobility shift assay,and Western blot assay.Protein expression levels of mitogen-activated protein kinase （c-Jun N-terminal kinase [JNK],extra-cellular signal-regulated kinase [ERK],and p38） were determined by Western blot assay.Results：After HCAECs were exposed to TNF-α,1-10 μtmol/L Sal B significantly inhibited TNF-α-induced MMP-9 expression and activity.Furthermore,Sal B significantly decreased IκBα phosphorylation and p65 nuclear translocation in HCAECs stimulated with TNF-α for 30 min.In addition,Sal B decreased the phosphorylation of JNK and ERK1/2 proteins in cells treated with TNF-α for 10 min.Conclusions：The data suggested that Sal B suppressed TNF-α-induced MMP-9 expression and activity by blocking the activation of NF-κB,JNK,and ERK1/2 signaling pathways.
基金This study was supported by National Natural Science Foundation of China(Nos.81202345,81771762,81170661 and 31640048)Nanjing Science and Technology Development Program(No.201503003)。
文摘Background Juvenile idiopathic arthritis(JIA)is the most common rheumatic disease in childhood driven by aberrant pathways of T-cell activation.T helper 17(Th17)/regulatory T cell(Treg)imbalance plays critical roles in the pathogenesis of arthritis.MicroRNA-125b(miR-125b)was upregulated after the activation of the initial CD4^+T cells,and could regulate the differentiation of CD4^+T cells.However,the effects of miR-125b on Th17/Treg imbalance and differentiation of Th 17/Treg cells remain unknown.Methods In this study,we evaluated the expression of miR-125b in the peripheral blood mononuclear cells(PBMCs)of children with JIA,and the relationship of miR-125b with Th17/Treg imbalance.Then,we used lentivirus vector-mediated overexpression technology to investigate the regulatory function of miR-125b in CD4^+T cells or dendritic cell/CD4^+T co-culture system.Results Decreased miR-125b expression in PBMCs and CD4^+T cells of JIA patients was negatively correlated with the ratio of Th17/Treg cells.It also correlated negatively with retinoic acid receptor-related orphan receptorγt but positively with Forkhead box protein 3 at transcriptional levels.Furthermore,we found that miR-125b overexpression inhibited Th17 cell differentiation,whereas facilitated the differentiation of Treg cells.MiR-125b upregulation led to the decrease of Th 17-secreting cytokines but the increase of the Treg-secreting cytokines.Conclusions Our results demonstrate that miR-125b participated in regulating Thl7/Treg cell differentiation and imbalance in JIA patients.These findings provide novel insight into the critical role of miR-125b in the Th17/Treg imbalance of JIA,and raise the distinct possibility that miR-125b may prove to be a potential therapeutic target for JIA.
基金supported by the National Natural Science Foundation of China(Grant No.41991252)the Strategic Priority Research Program(Grant No.XDB40000000)+4 种基金the International Partnership Program(Grant No.132B61KYSB20170005)of Chinese Academy of Sciencesthe National Natural Science Foundation of China(Grant No.41888101)partly supported by the USA National Science Foundation(Grant Nos.0908792,1211299&1702816 to R.Lawrence EDWARDS and Hai CHENG)the 111 Program of China(Grant No.D19002)the Belt&Road Center for Climate and Environment Studies of IEECAS。
文摘Evaluating anthropogenic impacts on regional vegetation changes during historical time is not only important for a better understanding of the Anthropocene but also valuable in improving the vegetation-climate models.In this study,we analyzed stable isotopes(δ^18O,δ^13C)and trace elements(Mg/Ca,Sr/Ca)of a stalagmite from Huangchao Cave in central Shandong,northern China.230Th and AMS14C dating results indicate the stalagmite deposited during 174BC and AD1810,with a hiatus between AD638 and 1102.Broad similarities of theδ^18O and trace elements in the stalagmite suggest they are reliable precipitation indexes.Theδ^13C of the stalagmite,a proxy of vegetation change,was generally consistent with local precipitation and temperature variations on a centennial-scale before the 15th century.It typically varied from–9.6‰to–6.3‰,indicating climate controlled C3 type vegetation during this period.However,a persistent and marked increasing trend in theδ^13C record was observed since the 15th century,resulting inδ^13C values from–7.7‰to–1.6‰in the next four centuries.This unprecedentedδ^13C change caused by vegetation deterioration cannot be explained by climate change but is fairly consistent with the dramatically increasing population and farmland in Shandong.We suggest that the increasing deforestation and reclamation in central Shandong began to affect vegetation in the mountain region of central Shandong since the 15th century and severely destroyed or even cleared the forest during the 16th–18th century.
基金supported by the Outstanding Youth Science Foundation of China(51922005)the National Natural Science Foundation of China(51775047 and 52105427)。
文摘With the accelerated development of electronic devices,micro-supercapacitors(MSCs),as energy storage devices that can charge and discharge quickly,have attracted considerable attention.To improve the rate capability of MSCs with consideration of the energy density remains a challenge.We demonstrated a facile method for the preparation of thin films through bottom-up femtosecond pulsed laser deposition.The femtosecond laser irradiated the polyimide film through a transparent substrate to uniformly sputter the electrode material onto the lower surface of the substrate.We successfully deposited porous amorphous carbon,graphene,and carbon quantum dots with controllable properties by temporally shaping the femtosecond laser.The resulting MSC exhibited an ultrahigh frequency response and good performance at scan rates up to 10,000 V s^(-1).The characteristic frequency f_(0) of the MSC was as high as 42,000 Hz,and the relaxation time constant τ_(0) was 0.0238 ms.The MSC reached an impedance phase angle of -82.6° at a frequency of 120 Hz,an ultrahigh power density of more than 30 kW cm^(-3),and an energy density of 0.068 W h cm-3.This method provides a novel perspective for the preparation of ultrahigh frequency filters for future miniaturized portable electronic devices.
基金partially supported by the National Key R&D Program of China (grant 2018YFC1200602 and 2016YFD0500403 to RHH)。
文摘Zika virus(ZIKV) is associated with severe birth defects and Guillain-Barre′ syndrome and no approved vaccines or specific therapies to combat ZIKV infection are currently available. To accelerate anti-ZIKV therapeutics research, we developed a stable ZIKV GFP-reporter virus system with considerably improved GFP visibility and stability. In this system a BHK-21 cell line expressing DC-SIGNR was established to facilitate the proliferation of GFP-reporter ZIKV. Using this reporter virus system, we established a high-throughput screening assay and screened a selected plant-sourced compounds library for their ability to block ZIKV infection. More than 31 out of 974 tested compounds effectively decreased ZIKV reporter infection. Four selected compounds, homoharringtonine(HHT), bruceine D(BD), dihydroartemisinin(DHA) and digitonin(DGT), were further validated to inhibit wild-type ZIKV infection in cells of BHK-21 and human cell line A549.The FDA-approved chronic myeloid leukemia treatment drug HHT and BD were identified as broad-spectrum flavivirus inhibitors. DHA, another FDA-approved antimalarial drug effectively inhibited ZIKV infection in BHK-21 cells. HHT, BD and DHA inhibited ZIKV infection at a post-entry stage. Digitonin was found to have inhibitory activity in the early stage of viral infection. Our research provides an efficient high-throughput screening assay for ZIKV inhibitors. The active compounds identified in this study represent potential therapies for the treatment of ZIKV infection.
基金This work is supported by the National Natural Science Foundation of China(Grant No.31760265 and 82160355)grant awarded by Natural Science Foundation of Jiangxi Province(20171ACB21036 and 20192ACB80008).
文摘The treatment and healing of infected skin lesions is one of the major challenges in surgery.To solve this problem,collagen I(Col-I)and the antibacterial agent hydroxypropyltrimethyl ammonium chloride chitosan(HACC)were composited into the bacterial cellulose(BC)three-dimensional network structure by a novel membrane-liquid interface(MLI)culture,and a Col-I/HACC/BC(CHBC)multifunctional dressing was designed.The water absorption rate and water vapor transmission rate of the obtained CHBC dressing were 35.78±2.45 g/g and 3084±56 g m^(-2)⋅day^(-1),respectively.The water retention of the CHBC dressing was significantly improved compared with the BC caused by the introduced Col-I and HACC.In vitro results indicated that the combined advantages of HACC and Col-I confer on CHBC dressings not only have outstanding antibacterial properties against Staphylococcus aureus(S.aureus)compared with BC and CBC,but also exhibit better cytocompatibility than BC and HBC to promote the proliferation and spread of NIH3T3 cells and HUVECs.Most importantly,the results of in vivo animal tests demonstrated that the CHBC dressings fully promoted wound healing for 8 days and exhibited shorter healing times,especially in the case of wound infection.Excellent skin regeneration effects and higher expression levels of collagen during infection were also shown in the CHBC group.We believe that CHBC composites with favorable multifunctionality have potential applications as wound dressings to treat infected wounds.
基金supported by the Key Program of National Natural Science Foundation of China(82030056)the National Key Research and Development Program(2016YFC1000805)Intramural Research Project Grants(AWS17J007 and 2018-JCJQ-ZQ-001).
文摘Radiotherapy is one of the main cancer treatments,but it may damage normal tissue and cause various side effects.At present,radioprotective agents used in clinics have side effects such as nausea,vomiting,diarrhea and hypotension,which limit their clinical application.It has been found that exosomes play an indispensable role in radiation injury.Exosomes are lipid bilayer vesicles that carry various bioactive substances,such as proteins,lipids and microRNA(miRNA),that play a key role in cell-to-cell communication and affect tissue injury and repair.In addition,studies have shown that radiation can increase the uptake of exosomes in cells and affect the composition and secretion of exosomes.Here,we review the existing studies and discuss the effects of radiation on exosomes and the role of exosomes in radiation injury,aiming to provide new insights for the treatment of radiation injury.
