Ovarian follicle development is associated with the physiological functions of granulosa cells(GCs),including proliferation and apoptosis.The level of miR-24-3p in ovarian tissue of high-yielding Yorkshire×Landra...Ovarian follicle development is associated with the physiological functions of granulosa cells(GCs),including proliferation and apoptosis.The level of miR-24-3p in ovarian tissue of high-yielding Yorkshire×Landrace sows was significantly higher than that of low-yielding sows.However,the functions of miR-24-3p on GCs are unclear.In this study,using flow cytometry,5-ethynyl-2′-de-oxyuridine(EdU)staining,and cell count,we showed that miR-24-3p promoted the proliferation of GCs increasing the proportion of cells in the S phase and upregulating the expression of cell cycle genes,moreover,miR-24-3p inhibited GC apoptosis.Mechanistically,on-line prediction,bioinformatics analysis,a luciferase reporter assay,RT-qPCR,and Western blot results showed that the target gene of miR-24-3p in proliferation and apoptosis is cyclin-dependent kinase inhibitor 1B(P27/CDKN1B).Furthermore,the effect of miR-24-3p on GC proliferation and apoptosis was attenuated by P27 overexpression.These findings suggest that miR-24-3p regulates the physiological functions of GCs.展开更多
We experimentally and theoretically present a paradigm for the accurate bilayer design of gradient metasurfaces for wave beam manipulation,producing an extremely asymmetric splitting effect by simply tailoring the int...We experimentally and theoretically present a paradigm for the accurate bilayer design of gradient metasurfaces for wave beam manipulation,producing an extremely asymmetric splitting effect by simply tailoring the interlayer size.This concept arises from anomalous diffraction in phase gradient metasurfaces and the precise combination of the phase gradient in bilayer metasurfaces.Ensured by different diffraction routes in momentum space for incident beams from opposite directions,extremely asymmetric acoustic beam splitting can be generated in a robust way,as demonstrated in experiments through a designed bilayer system.Our work provides a novel approach and feasible platform for designing tunable devices to control wave propagation.展开更多
Background:Oleic acid is an abundant free fatty acid present in livestock that are in a negative energy-balance state,and it may have detrimental effects on female reproduction and fertility.Oleic acid induces lipid a...Background:Oleic acid is an abundant free fatty acid present in livestock that are in a negative energy-balance state,and it may have detrimental effects on female reproduction and fertility.Oleic acid induces lipid accumulation in bovine granulosa cells,which leads to a foam cell-like morphology and reduced steroidogenesis.However,why oleic acid increases lipid accumulation but decreases steroidogenesis remains unclear.This study focused on oleic acid’s effects on lipid type and steroidogenesis.Results:Oleic acid increased the lipid accumulation in a concentration-dependent manner and mainly increased the triglyceride level and decreased the cholesterol ester level.Oleic acid also led to a decline in estradiol and progesterone production in porcine granulosa cells in vitro.In addition,oleic acid up-regulated the expression of CD36 and diacylglycerol acyltransferase 2,but down-regulated the expression of 3-hydroxy-3-methylglutarylcoenzyme A reductase,scavenger receptor class B member 1 and acetyl-Coenzyme A acetyltransferase 2,as well as steroidogenesis-related genes,including cytochrome P450 family 11 subfamily A member 1,cytochrome P450family 19 subfamily A member 1 and 3 as well as steroidogenic acute regulatory protein at the mRNA and protein levels.An oleic acid-rich diet also enhanced the triglyceride levels and reduced the cholesterol levels in ovarian tissues of female mice,which resulted in lower estradiol levels than in control-fed mice.Compared with the control,decreases in estrus days and the numbers of antral follicles and corpora lutea,as well as an increase in the numbers of the atretic follicles,were found in the oleic acid-fed female mice.Conclusions:Oleic acid changed the lipid type stored in lipid droplets of ovarian granulosa cells,and led to a decrease in steroidogenesis.These results improve our understanding of fertility decline in livestock that are in a negative energy-balance state.展开更多
Objective:This study aimed to compare the efficacy of anti-CD19 chimeric antigen receptor T cells(CAR-T cells)versus chemotherapy plus donor lymphocyte infusion(chemo-DLI)for treating relapsed CD 19-positive B-cell ac...Objective:This study aimed to compare the efficacy of anti-CD19 chimeric antigen receptor T cells(CAR-T cells)versus chemotherapy plus donor lymphocyte infusion(chemo-DLI)for treating relapsed CD 19-positive B-cell acute lymphoblastic leukemia(B-ALL)after allogeneic hematopoietic stem cell transplantation(allo-HSCT).Methods:Clinical data of 43 patients with B-ALL who relapsed after allo-HSCT were retrospectively analyzed.Twenty-two patients were treated with CAR-T cells(CAR-T group),and 21 with chemotherapy plus DLI(chemo-DLI group).The complete remission(CR)and minimal residual disease(MRD)-negative CR rates,leukemia-free survival(LFS)rate,overall survival(OS)rate,and incidence of acute graft-versus-host disease(aGVHD),cytokine release syndrome(CRS)and immune effector cell-associated neurotoxicity syndrome(ICANS)were compared between the two groups.Results:The CR and MRD-negative CR rates in the CAR-T group(77.3%and 61.5%)were significantly higher than those in the chemo-DLI group(38.1%and 23.8%)(P=0.008 and P=0.003).The 1-and 2-year LFS rates in the CAR-T group were superior to those in the chemo-DLI group:54.5%and 50.0%vs.9.5%and 4.8%(P=0.0001 and P=0.00004).The 1-and 2-year OS rates in the CAR-T versus chemo-DLI group were 59.1%and 54.5%vs.19%and 9.5%(P=0.011 and P=0.003).Six patients(28.6%)with grade 2-4 aGVHD were identified in the chemo-DLI group.Two patients(9.1%)in the CAR-T group developed grade 1-2 aGVHD.Nineteen patients(86.4%)developed CRS in the CAR-T group,comprising grade 1-2 CRS in 13 patients(59.1%)and grade 3 CRS in 6 patients(27.3%).Two patients(9.1%)developed grade 1-2 ICANS.Conclusion:Donor-derived anti-CD19 CAR-T-cell therapy may be better,safer,and more effective than chemo-DLI for B-ALL patients who relapse after allo-HSCT.展开更多
Background Clock circadian regulator(CLOCK)is a core factor of the mammalian biological clock system in regulat-ing female fertility and ovarian physiology.However,CLOCK’s specific function and molecular mechanism in...Background Clock circadian regulator(CLOCK)is a core factor of the mammalian biological clock system in regulat-ing female fertility and ovarian physiology.However,CLOCK’s specific function and molecular mechanism in porcine granulosa cells(GCs)remain unclear.In this study,we focused on CLOCK’s effects on GC proliferation.Results CLOCK significantly inhibited cell proliferation in porcine GCs.CLOCK decreased the expression of cell cycle-related genes,including CCNB1,CCNE1,and CDK4 at the mRNA and protein levels.CDKN1A levels were upregulated by CLOCK.ASB9 is a newly-identified target of CLOCK that inhibits GC proliferation;CLOCK binds to the E-box element in the ASB9 promoter.Conclusions These findings suggest that CLOCK inhibits the proliferation of porcine ovarian GCs by increasing ASB9 level.展开更多
Yellow seed trait is a desirable characteristic with potential for increasing seed quality and commercial value in rapeseed,and anthocyanin and proanthocyanidins(PAs)are major seed-coat pigments.Few transcription fact...Yellow seed trait is a desirable characteristic with potential for increasing seed quality and commercial value in rapeseed,and anthocyanin and proanthocyanidins(PAs)are major seed-coat pigments.Few transcription factors involved in the regulation of anthocyanin and PAs biosynthesis have been characterized in rapeseed.In this study,we identified a transcription factor gene BnbHLH92a(BnaA06T0441000ZS)in rapeseed.Overexpressing BnbHLH92a both in Arabidopsis and in rapeseed reduced levels of anthocyanin and PAs.Correspondingly,the expression profiles of anthocyanin and PA biosynthesis genes(TT3,BAN,TT8,TT18,and TTG1)were shown by quantitative real-time PCR to be inhibited in BnbHLH92a-overexpressing Arabidopsis seeds,indicating that BnbHLH92a represses the anthocyanin and PA biosynthesis pathway in Arabidopsis.BnbHLH92a physically interacts with the BnTTG1 protein and represses the biosynthesis of anthocyanins and PAs in rapeseed.BnbHLH92a also binds directly to the BnTT18 promoter and represses its expression.These results suggest that BnbHLH92a is a novel upstream regulator of flavonoid biosynthesis in B.napus.展开更多
Background:Delirium is a neurocognitive disorder characterized by an abrupt decline in attention,awareness,and cognition after surgical/illness-induced stressors on the brain.There is now an increasing focus on how ca...Background:Delirium is a neurocognitive disorder characterized by an abrupt decline in attention,awareness,and cognition after surgical/illness-induced stressors on the brain.There is now an increasing focus on how cardiovascular health interacts with neurocognitive disorders given their overlapping risk factors and links to subsequent dementia and mortality.One common indicator for cardiovascular health is the heart rate response/recovery(HRR)to exercise,but how this relates to future delirium is unknown.Methods:Electrocardiogram data were examined in 38,740 middle-to older-aged UK Biobank participants(mean age=58.1 years,range:40-72 years;47.3%males)who completed a standardized submaximal exercise stress test(15-s baseline,6-min exercise,and 1-min recovery)and required hospitalization during follow-up.An HRR index was derived as the product of the heart rate(HR)responses during exercise(peak/resting HRs)and recovery(peak/recovery HRs)and categorized into low/average/high groups as the bottom quartile/middle 2 quartiles/top quartile,respectively.Associations between 3 HRR groups and new-onset delirium were investigated using Cox proportional hazards models and a2-year landmark analysis to minimize reverse causation.Sociodemographic factors,lifestyle factors/physical activity,cardiovascular risk,comorbidities,cognition,and maximal workload achieved were included as covariates.Results:During a median follow-up period of 11 years,348 participants(9/1000)newly developed delirium.Compared with the high HRR group(16/1000),the risk for delirium was almost doubled in those with low HRR(hazard ratio=1.90,95%)confidence interval(95%CI):1.30-2.79,p=0.001)and average HRR(hazard ratio=1.54,95%CI:1.07-2.22,p=0.020)).Low HRR was equivalent to being 6 years older,a current smoker,or>3 additional cardiovascular disease risks.Results were robust in sensitivity analysis,but the risk appeared larger in those with better cognition and when only postoperative delirium was considered(n=147;hazard ratio=2.66,95%CI:1.46-4.85,p=0.001).Conclusion:HRR during submaximal exercise is associated with future risk for delirium.Given that HRR is potentially modifiable,it may prove useful for neurological risk stratification alongside traditional cardiovascular risk factors.展开更多
The development of all-solid-state lithium batteries(ASSLBs)depends on exploiting solid-state electrolytes(SSEs)with high ionic conductivity and electrochemical stability.Fluorination is generally considered to be an ...The development of all-solid-state lithium batteries(ASSLBs)depends on exploiting solid-state electrolytes(SSEs)with high ionic conductivity and electrochemical stability.Fluorination is generally considered to be an effective strategy to improve the ionic conductivity and electrochemical stability of inorganic SSEs.Here,we report the partial fluorination of the chlo rine sites in an antiperovskite,by which the orthorhombic Li_(2)OHCl was transformed into cubic Li_(2)OHCl_(0.9)F_(0.1),resulting in a fourfold increase in ionic conductivity at 30℃.The ab initio molecular dynamics simulations suggest that both the crystal symmetry and the anions electronegativity influence the diffusion of Li+in the antiperovskite structure.Besides,from the perspective of experiments and calculations,it is confirmed that fluorination is a feasible method to improve the electrochemical stability of antiperovskite SSEs.The LiFePO_(4)|Li cell based on Li_(2)OHCl_(0.9)F_(0.1) is also assembled and exhibits stable cycle performance,which indicates that fluorination of antiperovskite SSEs is an effective way to produce high-performance SSEs for practical application of ASSLBs.展开更多
Ultra-high spectral purity lasers are of considerable research interests in numerous fields such as coherent optical communication,microwave photonics,distributed optical fiber sensing,gravitational wave detection,opt...Ultra-high spectral purity lasers are of considerable research interests in numerous fields such as coherent optical communication,microwave photonics,distributed optical fiber sensing,gravitational wave detection,optical clock,and so on.Herein,to deeply purify laser spectrum with compact size under normal condition,we propose a novel and practical idea to effectively suppress the spontaneous radiation of the laser cavity through weak external distributed perturbation.Subsequently,a laser configuration consisting of a main lasing cavity and an external distributed feedback cavity is proposed.The feedback signal with continuous spatio-temporal phase transition controlled by a distributed feedback structure is injected into the main cavity,which can deeply suppress the coupling rate from the spontaneous radiation to the stimulated emission and extremely purify the laser spectrum.Eventually,an ultra-narrow linewidth on-chip laser system with a side mode suppression ratio greater than 80 dB,an output linewidth of 10 Hz,and a relative intensity noise less than-150 dB/Hz is successfully obtained under normal conditions.The proposed concept in this work provides a new perspective for extreme regulation of laser parameters by using weak external distributed perturbation,which can be valid for various gain-type lasers with wide wavelength bands.展开更多
Bamboo is an eco-friendly material with light weight,high strength,short growth cycle and high sustainability,which is widely used in building structures.Engineered bamboo has further promoted the development of moder...Bamboo is an eco-friendly material with light weight,high strength,short growth cycle and high sustainability,which is widely used in building structures.Engineered bamboo has further promoted the development of modern bamboo structures due to its unrestricted size and shape.However,as a fiber-reinforced material,fracture damage,especially Mode I fracture damage,becomes the most likely damage mode of its structure,so Mode I fracture characteristics are an important subject in the research of mechanical properties of bamboo.This paper summarizes the current status of experimental research on the Mode I fracture properties of bamboo based on the three-point bending(TPB)method,the single-edge notched beam(SENB)method,the compact tension(CT)method and the double cantilever beam(DCB)method,compares the fracture toughness of different species of bamboo,analyzes the toughening mechanisms and fracture damage modes,discusses the applicability of different theoretical calculation methods,and makes suggestions for future research priorities,aiming to provide a reference for future research and engineering applications in related fields.展开更多
The on-surface synthesis method allows the fabrication of atomically precise narrow graphene nanoribbons(GNRs),which bears great potential in electronic applications.Here,we synthesize armchair graphene nanoribbons(AG...The on-surface synthesis method allows the fabrication of atomically precise narrow graphene nanoribbons(GNRs),which bears great potential in electronic applications.Here,we synthesize armchair graphene nanoribbons(AGNRs)and chevron-type graphene nanoribbons(CGNRs)array on a vicinal Au(111112)surface using 10,10′-dibromo-9,9′-bianthracene(DBBA)and 6,12-dibromochrysene(DBCh)as precursors,respectively.This process creates spatially wellaligned GNRs,as characterized by scanning tunneling microscopy.AGNRs show strong Raman linear polarizability for application in optical modulation devices.Different from the distinct polarization of AGNRs,only weak polarization exists in CGNRs polarized Raman spectrum,which suggests that the presence of the zigzag boundary in the nanoribbon attenuates the polarization rate as an important factor affecting the polarization.We analyze the Raman activation mode of CGNRs using the peak polarization to expand the application of the polarization Raman spectroscopy in nanoarray analysis.展开更多
Based on the many-body radiative heat transfer theory,we investigate a thermal splitter based on three magneto-optical In Sb nanoparticles.The system comprises a source with adjustable parameters and two drains with f...Based on the many-body radiative heat transfer theory,we investigate a thermal splitter based on three magneto-optical In Sb nanoparticles.The system comprises a source with adjustable parameters and two drains with fixed parameters.By leveraging the temperature and magnetic field dependence of the permittivity of In Sb,the direction of heat flux in the system can be controlled by adjusting the magnetic field or temperature at the source.Under magnetic field control,the coupling between the separated modes,and the suppression of the zero-field mode induced by the magnetic field,are utilized to achieve a thermal splitting ratio within the modulation range of 0.15–0.58.Furthermore,temperature control results in a thermal splitting ratio ranging from 0.15 to 0.99,as a result of the suppression of the zero-field mode by the magnetic field and the blue shift effect of the zero-field mode frequency increasing with temperature.Notably,the gap distance between nanoparticles does not significantly affect the splitting ratio.These findings provide valuable theoretical guidance for utilizing magneto-optical nanoparticles as thermal splitters and lay the groundwork for implementing complex heat flux networks using In Sb for energy collection and heat transfer control.展开更多
Near-field radiative heat transfer(NFRHT) research is an important research project after a major breakthrough in nanotechnology. Based on the multilayer structure, we find that due to the existence of inherent losses...Near-field radiative heat transfer(NFRHT) research is an important research project after a major breakthrough in nanotechnology. Based on the multilayer structure, we find that due to the existence of inherent losses,the decoupling of hyperbolic modes(HMs) after changing the filling ratio leads to suppression of heat flow near the surface mode resonance frequency. It complements the physical landscape of enhancement of near-field radiative heat transfer by HMs and more surface states supported by multiple surfaces. More importantly, considering the difficulty of accurate preparation at the nanoscale, we introduce the disorder factor to describe the magnitude of the random variation of the layer thickness of the multilayer structure and then explore the effect on heat transfer when the layer thickness is slightly different from the exact value expected. We find that the near-field radiative heat flux decreases gradually as the disorder increases because of interlayer energy localization. However, the reduction in heat transfer does not exceed an order of magnitude, although the disorder is already very large. At the same time, the regulation effect of the disorder on NFRHT is close to that of the same degree of filling ratio,which highlights the importance of disordered systems. This work qualitatively describes the effect of disorder on heat transfer and provides instructive data for the fabrication of NFRHT devices.展开更多
BACKGROUND Peptic ulcer(PU)is an abnormal phenomenon in which there is rupture of the mucosa of the digestive tract,which not only affects patients'normal life but also causes an economic burden due to its high me...BACKGROUND Peptic ulcer(PU)is an abnormal phenomenon in which there is rupture of the mucosa of the digestive tract,which not only affects patients'normal life but also causes an economic burden due to its high medical costs.AIM To investigate the efficacy of pantoprazole(PPZ)plus perforation repair in patients with PU and its effect on the stress response.METHODS The study subjects were 108 PU patients admitted between July 2018 and July 2022,including 58 patients receiving PPZ plus perforation repair[research group(RG)]and 50 patients given simple perforation repair[control group(CG)].The efficacy,somatostatin(SS)concentration,stress reaction[malondialdehyde(MDA),lipid peroxide(LPO)],inflammatory indices[tumor necrosis factor(TNF)-α,C-reactive protein(CRP),interleukin(IL)-1β],recurrence,and complications(perforation,hemorrhage,and pyloric obstruction)were compared.RESULTS The overall response rate was higher in the RG than in the CG.Patients in the RG and IL-1β were significantly reduced to lower levels than those in the CG.Lower recurrence and complication rates were identified in the RG group.CONCLUSION Therefore,PPZ plus perforation repair is conducive to enhancing treatment outcomes in PU patients,reducing oxidative stress injury and excessive inflammatory reactions,and contributing to low recurrence and complication rates.展开更多
[Objectives]To explore the effects of continuous cropping and crop rotation on fungal diversity in greenhouse soil.[Methods]Using the greenhouse soil grown with Ganoderma leucocontextum in different continuous(rotatio...[Objectives]To explore the effects of continuous cropping and crop rotation on fungal diversity in greenhouse soil.[Methods]Using the greenhouse soil grown with Ganoderma leucocontextum in different continuous(rotation)cropping years as experimental materials,the change trend of soil fungal diversity in different continuous(rotation)cropping years was studied.Through high-throughput sequencing technology combined with related bioinformatics methods,the richness,diversity index and community structure of the fungal ITS1+ITS2 region in the soil were analyzed.[Results]Continuous cropping or crop rotation could increase the diversity of microbial communities.Ascomycota,Basidiomycota and Zygomycota were dominant fungi.The proportion of Basidiomycota gradually increased with the increase of continuous cropping years,and the abundance decreased after crop rotation.[Conclusions]This study is intended to provide a theoretical basis for the research on continuous cropping and crop rotation obstacles.展开更多
基金supported by the National Natural Science Foundation of China(32272849)the National Key R&D Program of China(2021YFF1000602)the earmarked fund for CARS-35-PIG。
文摘Ovarian follicle development is associated with the physiological functions of granulosa cells(GCs),including proliferation and apoptosis.The level of miR-24-3p in ovarian tissue of high-yielding Yorkshire×Landrace sows was significantly higher than that of low-yielding sows.However,the functions of miR-24-3p on GCs are unclear.In this study,using flow cytometry,5-ethynyl-2′-de-oxyuridine(EdU)staining,and cell count,we showed that miR-24-3p promoted the proliferation of GCs increasing the proportion of cells in the S phase and upregulating the expression of cell cycle genes,moreover,miR-24-3p inhibited GC apoptosis.Mechanistically,on-line prediction,bioinformatics analysis,a luciferase reporter assay,RT-qPCR,and Western blot results showed that the target gene of miR-24-3p in proliferation and apoptosis is cyclin-dependent kinase inhibitor 1B(P27/CDKN1B).Furthermore,the effect of miR-24-3p on GC proliferation and apoptosis was attenuated by P27 overexpression.These findings suggest that miR-24-3p regulates the physiological functions of GCs.
文摘We experimentally and theoretically present a paradigm for the accurate bilayer design of gradient metasurfaces for wave beam manipulation,producing an extremely asymmetric splitting effect by simply tailoring the interlayer size.This concept arises from anomalous diffraction in phase gradient metasurfaces and the precise combination of the phase gradient in bilayer metasurfaces.Ensured by different diffraction routes in momentum space for incident beams from opposite directions,extremely asymmetric acoustic beam splitting can be generated in a robust way,as demonstrated in experiments through a designed bilayer system.Our work provides a novel approach and feasible platform for designing tunable devices to control wave propagation.
基金supported by grants from the National Natural Science Foundation(No.31802047)the National Science and Technology Major Project of China(No.2016ZX08006003)。
文摘Background:Oleic acid is an abundant free fatty acid present in livestock that are in a negative energy-balance state,and it may have detrimental effects on female reproduction and fertility.Oleic acid induces lipid accumulation in bovine granulosa cells,which leads to a foam cell-like morphology and reduced steroidogenesis.However,why oleic acid increases lipid accumulation but decreases steroidogenesis remains unclear.This study focused on oleic acid’s effects on lipid type and steroidogenesis.Results:Oleic acid increased the lipid accumulation in a concentration-dependent manner and mainly increased the triglyceride level and decreased the cholesterol ester level.Oleic acid also led to a decline in estradiol and progesterone production in porcine granulosa cells in vitro.In addition,oleic acid up-regulated the expression of CD36 and diacylglycerol acyltransferase 2,but down-regulated the expression of 3-hydroxy-3-methylglutarylcoenzyme A reductase,scavenger receptor class B member 1 and acetyl-Coenzyme A acetyltransferase 2,as well as steroidogenesis-related genes,including cytochrome P450 family 11 subfamily A member 1,cytochrome P450family 19 subfamily A member 1 and 3 as well as steroidogenic acute regulatory protein at the mRNA and protein levels.An oleic acid-rich diet also enhanced the triglyceride levels and reduced the cholesterol levels in ovarian tissues of female mice,which resulted in lower estradiol levels than in control-fed mice.Compared with the control,decreases in estrus days and the numbers of antral follicles and corpora lutea,as well as an increase in the numbers of the atretic follicles,were found in the oleic acid-fed female mice.Conclusions:Oleic acid changed the lipid type stored in lipid droplets of ovarian granulosa cells,and led to a decrease in steroidogenesis.These results improve our understanding of fertility decline in livestock that are in a negative energy-balance state.
基金supported by grants from the National Natural Science Foundation of China(No.82020108004)the Hospital-level Clinical Innovation Military-Civilian Special Project of Army Medical University(No.2018JSLC0020)+1 种基金Chongqing Science and Technology Innovation Leading Talent(No.CSTCCXLJRC201718)Natural Science Foundation of Chongqing Innovation Group Science Program(No.cstc2021jcyj-cxttX0001).
文摘Objective:This study aimed to compare the efficacy of anti-CD19 chimeric antigen receptor T cells(CAR-T cells)versus chemotherapy plus donor lymphocyte infusion(chemo-DLI)for treating relapsed CD 19-positive B-cell acute lymphoblastic leukemia(B-ALL)after allogeneic hematopoietic stem cell transplantation(allo-HSCT).Methods:Clinical data of 43 patients with B-ALL who relapsed after allo-HSCT were retrospectively analyzed.Twenty-two patients were treated with CAR-T cells(CAR-T group),and 21 with chemotherapy plus DLI(chemo-DLI group).The complete remission(CR)and minimal residual disease(MRD)-negative CR rates,leukemia-free survival(LFS)rate,overall survival(OS)rate,and incidence of acute graft-versus-host disease(aGVHD),cytokine release syndrome(CRS)and immune effector cell-associated neurotoxicity syndrome(ICANS)were compared between the two groups.Results:The CR and MRD-negative CR rates in the CAR-T group(77.3%and 61.5%)were significantly higher than those in the chemo-DLI group(38.1%and 23.8%)(P=0.008 and P=0.003).The 1-and 2-year LFS rates in the CAR-T group were superior to those in the chemo-DLI group:54.5%and 50.0%vs.9.5%and 4.8%(P=0.0001 and P=0.00004).The 1-and 2-year OS rates in the CAR-T versus chemo-DLI group were 59.1%and 54.5%vs.19%and 9.5%(P=0.011 and P=0.003).Six patients(28.6%)with grade 2-4 aGVHD were identified in the chemo-DLI group.Two patients(9.1%)in the CAR-T group developed grade 1-2 aGVHD.Nineteen patients(86.4%)developed CRS in the CAR-T group,comprising grade 1-2 CRS in 13 patients(59.1%)and grade 3 CRS in 6 patients(27.3%).Two patients(9.1%)developed grade 1-2 ICANS.Conclusion:Donor-derived anti-CD19 CAR-T-cell therapy may be better,safer,and more effective than chemo-DLI for B-ALL patients who relapse after allo-HSCT.
基金supported by National Natural Science Foundation of China (32272849)China Agriculture Research System of MOF and MARA
文摘Background Clock circadian regulator(CLOCK)is a core factor of the mammalian biological clock system in regulat-ing female fertility and ovarian physiology.However,CLOCK’s specific function and molecular mechanism in porcine granulosa cells(GCs)remain unclear.In this study,we focused on CLOCK’s effects on GC proliferation.Results CLOCK significantly inhibited cell proliferation in porcine GCs.CLOCK decreased the expression of cell cycle-related genes,including CCNB1,CCNE1,and CDK4 at the mRNA and protein levels.CDKN1A levels were upregulated by CLOCK.ASB9 is a newly-identified target of CLOCK that inhibits GC proliferation;CLOCK binds to the E-box element in the ASB9 promoter.Conclusions These findings suggest that CLOCK inhibits the proliferation of porcine ovarian GCs by increasing ASB9 level.
基金supported by the National Natural Science Foundation of China(32072093,31830067)the China Agriculture Research System of MOF and MARA,the Science and Enterprise Consortium Project of Chongqing(cqnyncw-kqlhtxm)+1 种基金the Innovation and Entrepreneurship Training Program for Undergraduates(S202010635197)the 111 Project(B12006).
文摘Yellow seed trait is a desirable characteristic with potential for increasing seed quality and commercial value in rapeseed,and anthocyanin and proanthocyanidins(PAs)are major seed-coat pigments.Few transcription factors involved in the regulation of anthocyanin and PAs biosynthesis have been characterized in rapeseed.In this study,we identified a transcription factor gene BnbHLH92a(BnaA06T0441000ZS)in rapeseed.Overexpressing BnbHLH92a both in Arabidopsis and in rapeseed reduced levels of anthocyanin and PAs.Correspondingly,the expression profiles of anthocyanin and PA biosynthesis genes(TT3,BAN,TT8,TT18,and TTG1)were shown by quantitative real-time PCR to be inhibited in BnbHLH92a-overexpressing Arabidopsis seeds,indicating that BnbHLH92a represses the anthocyanin and PA biosynthesis pathway in Arabidopsis.BnbHLH92a physically interacts with the BnTTG1 protein and represses the biosynthesis of anthocyanins and PAs in rapeseed.BnbHLH92a also binds directly to the BnTT18 promoter and represses its expression.These results suggest that BnbHLH92a is a novel upstream regulator of flavonoid biosynthesis in B.napus.
基金funded by National Institutes of Health(NIH)Grant R03AG067985Foundation for Anesthesia Education and Research+1 种基金funded by the BrightFocus Foundation Alzheimer’s Disease Research Program(A2020886S)funded by NIH Grants RF1AG059867 and RF1AG064312,funded by NIH Grant R01HL140574。
文摘Background:Delirium is a neurocognitive disorder characterized by an abrupt decline in attention,awareness,and cognition after surgical/illness-induced stressors on the brain.There is now an increasing focus on how cardiovascular health interacts with neurocognitive disorders given their overlapping risk factors and links to subsequent dementia and mortality.One common indicator for cardiovascular health is the heart rate response/recovery(HRR)to exercise,but how this relates to future delirium is unknown.Methods:Electrocardiogram data were examined in 38,740 middle-to older-aged UK Biobank participants(mean age=58.1 years,range:40-72 years;47.3%males)who completed a standardized submaximal exercise stress test(15-s baseline,6-min exercise,and 1-min recovery)and required hospitalization during follow-up.An HRR index was derived as the product of the heart rate(HR)responses during exercise(peak/resting HRs)and recovery(peak/recovery HRs)and categorized into low/average/high groups as the bottom quartile/middle 2 quartiles/top quartile,respectively.Associations between 3 HRR groups and new-onset delirium were investigated using Cox proportional hazards models and a2-year landmark analysis to minimize reverse causation.Sociodemographic factors,lifestyle factors/physical activity,cardiovascular risk,comorbidities,cognition,and maximal workload achieved were included as covariates.Results:During a median follow-up period of 11 years,348 participants(9/1000)newly developed delirium.Compared with the high HRR group(16/1000),the risk for delirium was almost doubled in those with low HRR(hazard ratio=1.90,95%)confidence interval(95%CI):1.30-2.79,p=0.001)and average HRR(hazard ratio=1.54,95%CI:1.07-2.22,p=0.020)).Low HRR was equivalent to being 6 years older,a current smoker,or>3 additional cardiovascular disease risks.Results were robust in sensitivity analysis,but the risk appeared larger in those with better cognition and when only postoperative delirium was considered(n=147;hazard ratio=2.66,95%CI:1.46-4.85,p=0.001).Conclusion:HRR during submaximal exercise is associated with future risk for delirium.Given that HRR is potentially modifiable,it may prove useful for neurological risk stratification alongside traditional cardiovascular risk factors.
基金supported by the National Key Research and Development Program of China(2020YFA0210701)the National Natural Science Foundation of China(22005134,12275119,U22A20439)+4 种基金the Shenzhen Science and Technology Program(RCBS20210609103647030)the Guangdong Basic and Applied Basic Research Foundation(2021A1515012403)the Shenzhen Science and Technology Program(KQTD20200820113047086)the Guangdong-Hong Kong-Macao Joint Laboratory for PhotonicThermal-Electrical Energy Materials and Devices(2019B121205001)the open research fund of Songshan Lake Materials Laboratory(2022SLABFK04)。
文摘The development of all-solid-state lithium batteries(ASSLBs)depends on exploiting solid-state electrolytes(SSEs)with high ionic conductivity and electrochemical stability.Fluorination is generally considered to be an effective strategy to improve the ionic conductivity and electrochemical stability of inorganic SSEs.Here,we report the partial fluorination of the chlo rine sites in an antiperovskite,by which the orthorhombic Li_(2)OHCl was transformed into cubic Li_(2)OHCl_(0.9)F_(0.1),resulting in a fourfold increase in ionic conductivity at 30℃.The ab initio molecular dynamics simulations suggest that both the crystal symmetry and the anions electronegativity influence the diffusion of Li+in the antiperovskite structure.Besides,from the perspective of experiments and calculations,it is confirmed that fluorination is a feasible method to improve the electrochemical stability of antiperovskite SSEs.The LiFePO_(4)|Li cell based on Li_(2)OHCl_(0.9)F_(0.1) is also assembled and exhibits stable cycle performance,which indicates that fluorination of antiperovskite SSEs is an effective way to produce high-performance SSEs for practical application of ASSLBs.
基金supported by the National Natural Science Foundation of China(NSFC)(61635004)the National Science Fund for Distinguished Young Scholars(61825501)the Chongqing Natural Science Foundation of Innovative Research Groups under Grant(CSTC2020JCYJ,CXTTX0005)。
文摘Ultra-high spectral purity lasers are of considerable research interests in numerous fields such as coherent optical communication,microwave photonics,distributed optical fiber sensing,gravitational wave detection,optical clock,and so on.Herein,to deeply purify laser spectrum with compact size under normal condition,we propose a novel and practical idea to effectively suppress the spontaneous radiation of the laser cavity through weak external distributed perturbation.Subsequently,a laser configuration consisting of a main lasing cavity and an external distributed feedback cavity is proposed.The feedback signal with continuous spatio-temporal phase transition controlled by a distributed feedback structure is injected into the main cavity,which can deeply suppress the coupling rate from the spontaneous radiation to the stimulated emission and extremely purify the laser spectrum.Eventually,an ultra-narrow linewidth on-chip laser system with a side mode suppression ratio greater than 80 dB,an output linewidth of 10 Hz,and a relative intensity noise less than-150 dB/Hz is successfully obtained under normal conditions.The proposed concept in this work provides a new perspective for extreme regulation of laser parameters by using weak external distributed perturbation,which can be valid for various gain-type lasers with wide wavelength bands.
基金This work was supported by the National Natural Science Foundation of China(Nos.51878354&51308301)the Natural Science Foundation of Jiangsu Province(Nos.BK20181402&BK20130978)+3 种基金333 Talent High-Level Project of Jiangsu ProvinceQinglan Project of Jiangsu Higher Education Institutionsand the Ministry of Housing and Urban-Rural Science Project of Jiangsu Province under Grant(No.2021ZD10)Any research results expressed in this paper are those of the writer(s)and do not necessarily reflect the views of the foundations.
文摘Bamboo is an eco-friendly material with light weight,high strength,short growth cycle and high sustainability,which is widely used in building structures.Engineered bamboo has further promoted the development of modern bamboo structures due to its unrestricted size and shape.However,as a fiber-reinforced material,fracture damage,especially Mode I fracture damage,becomes the most likely damage mode of its structure,so Mode I fracture characteristics are an important subject in the research of mechanical properties of bamboo.This paper summarizes the current status of experimental research on the Mode I fracture properties of bamboo based on the three-point bending(TPB)method,the single-edge notched beam(SENB)method,the compact tension(CT)method and the double cantilever beam(DCB)method,compares the fracture toughness of different species of bamboo,analyzes the toughening mechanisms and fracture damage modes,discusses the applicability of different theoretical calculation methods,and makes suggestions for future research priorities,aiming to provide a reference for future research and engineering applications in related fields.
基金Project supported by the National Natural Science Foundation of China(Grant No.61901200)the Yunnan Fundamental Research Projects(Grant Nos.2019FD041,202101AU070043,202101AV070008+2 种基金202101AW070010)the Strategic Priority Research Program of Chinese Academy of Sciences(Grant No.NXDB30010000)the Dongguan Innovation Research Team Program。
文摘The on-surface synthesis method allows the fabrication of atomically precise narrow graphene nanoribbons(GNRs),which bears great potential in electronic applications.Here,we synthesize armchair graphene nanoribbons(AGNRs)and chevron-type graphene nanoribbons(CGNRs)array on a vicinal Au(111112)surface using 10,10′-dibromo-9,9′-bianthracene(DBBA)and 6,12-dibromochrysene(DBCh)as precursors,respectively.This process creates spatially wellaligned GNRs,as characterized by scanning tunneling microscopy.AGNRs show strong Raman linear polarizability for application in optical modulation devices.Different from the distinct polarization of AGNRs,only weak polarization exists in CGNRs polarized Raman spectrum,which suggests that the presence of the zigzag boundary in the nanoribbon attenuates the polarization rate as an important factor affecting the polarization.We analyze the Raman activation mode of CGNRs using the peak polarization to expand the application of the polarization Raman spectroscopy in nanoarray analysis.
基金supported by the National Natural Science Foundation of China(Grant Nos.92050104,12274314,12174281,and 52106099)the Natural Science Foundation of Jiangsu Province(Grant No.BK20221240)+1 种基金the Natural Science Foundation of Shandong Province(Grant No.ZR2022YQ57)the Taishan Scholars Program。
文摘Based on the many-body radiative heat transfer theory,we investigate a thermal splitter based on three magneto-optical In Sb nanoparticles.The system comprises a source with adjustable parameters and two drains with fixed parameters.By leveraging the temperature and magnetic field dependence of the permittivity of In Sb,the direction of heat flux in the system can be controlled by adjusting the magnetic field or temperature at the source.Under magnetic field control,the coupling between the separated modes,and the suppression of the zero-field mode induced by the magnetic field,are utilized to achieve a thermal splitting ratio within the modulation range of 0.15–0.58.Furthermore,temperature control results in a thermal splitting ratio ranging from 0.15 to 0.99,as a result of the suppression of the zero-field mode by the magnetic field and the blue shift effect of the zero-field mode frequency increasing with temperature.Notably,the gap distance between nanoparticles does not significantly affect the splitting ratio.These findings provide valuable theoretical guidance for utilizing magneto-optical nanoparticles as thermal splitters and lay the groundwork for implementing complex heat flux networks using In Sb for energy collection and heat transfer control.
基金the National Natural Science Foundation of China(Grant Nos.11974010 and 12274313)the National Key R&D Program of China(Grant Nos.2022YFA1404400 and 2022YFA1404300)。
文摘Near-field radiative heat transfer(NFRHT) research is an important research project after a major breakthrough in nanotechnology. Based on the multilayer structure, we find that due to the existence of inherent losses,the decoupling of hyperbolic modes(HMs) after changing the filling ratio leads to suppression of heat flow near the surface mode resonance frequency. It complements the physical landscape of enhancement of near-field radiative heat transfer by HMs and more surface states supported by multiple surfaces. More importantly, considering the difficulty of accurate preparation at the nanoscale, we introduce the disorder factor to describe the magnitude of the random variation of the layer thickness of the multilayer structure and then explore the effect on heat transfer when the layer thickness is slightly different from the exact value expected. We find that the near-field radiative heat flux decreases gradually as the disorder increases because of interlayer energy localization. However, the reduction in heat transfer does not exceed an order of magnitude, although the disorder is already very large. At the same time, the regulation effect of the disorder on NFRHT is close to that of the same degree of filling ratio,which highlights the importance of disordered systems. This work qualitatively describes the effect of disorder on heat transfer and provides instructive data for the fabrication of NFRHT devices.
文摘BACKGROUND Peptic ulcer(PU)is an abnormal phenomenon in which there is rupture of the mucosa of the digestive tract,which not only affects patients'normal life but also causes an economic burden due to its high medical costs.AIM To investigate the efficacy of pantoprazole(PPZ)plus perforation repair in patients with PU and its effect on the stress response.METHODS The study subjects were 108 PU patients admitted between July 2018 and July 2022,including 58 patients receiving PPZ plus perforation repair[research group(RG)]and 50 patients given simple perforation repair[control group(CG)].The efficacy,somatostatin(SS)concentration,stress reaction[malondialdehyde(MDA),lipid peroxide(LPO)],inflammatory indices[tumor necrosis factor(TNF)-α,C-reactive protein(CRP),interleukin(IL)-1β],recurrence,and complications(perforation,hemorrhage,and pyloric obstruction)were compared.RESULTS The overall response rate was higher in the RG than in the CG.Patients in the RG and IL-1β were significantly reduced to lower levels than those in the CG.Lower recurrence and complication rates were identified in the RG group.CONCLUSION Therefore,PPZ plus perforation repair is conducive to enhancing treatment outcomes in PU patients,reducing oxidative stress injury and excessive inflammatory reactions,and contributing to low recurrence and complication rates.
文摘[Objectives]To explore the effects of continuous cropping and crop rotation on fungal diversity in greenhouse soil.[Methods]Using the greenhouse soil grown with Ganoderma leucocontextum in different continuous(rotation)cropping years as experimental materials,the change trend of soil fungal diversity in different continuous(rotation)cropping years was studied.Through high-throughput sequencing technology combined with related bioinformatics methods,the richness,diversity index and community structure of the fungal ITS1+ITS2 region in the soil were analyzed.[Results]Continuous cropping or crop rotation could increase the diversity of microbial communities.Ascomycota,Basidiomycota and Zygomycota were dominant fungi.The proportion of Basidiomycota gradually increased with the increase of continuous cropping years,and the abundance decreased after crop rotation.[Conclusions]This study is intended to provide a theoretical basis for the research on continuous cropping and crop rotation obstacles.
