Background: The deep understanding of pathogenesis is a key moment in the formation of the modern strategy of modern medicine. We conducted the thorough analysis of the microscopic processes occurring in the bodies of...Background: The deep understanding of pathogenesis is a key moment in the formation of the modern strategy of modern medicine. We conducted the thorough analysis of the microscopic processes occurring in the bodies of patients with purulent-septic complications. The modified pathogenetic concept of the diagnostic and treatment model of diseases with septic complications is presented. The obtained information about the mechanisms of origin and development of these diseases is fundamentally important for finding the modern effective methods of treating patients. The aim of the research is to modify treatment tactics for patients with sepsis and burn injuries based on the modified pathogenetic concept using modern diagnostics, i.e. the method of fluorescence spectroscopy (MFS) and biomarkers. Materials and Methods: The proposed modified pathogenetic concept of the diagnostic and treatment model of diseases with purulent-septic complications along with standard methods was used successfully for effective treatment of 15 patients with sepsis and 25 with burn injuries. Results: 3 main scenarios of behaviour of spectral-fluorescence characteristics of patients with sepsis are illustrated. Spectral-fluorescence markers of sepsis were studied, which are informative 24 to 48 hours before the appearance of obvious clinical and laboratory signs of significant changes in the general somatic status of patients. Conclusions: The proposed diagnostic and therapeutic approach is new and fundamentally important for diagnostics and monitoring of the process of treatment of patients with purulent-septic diseases and burn injuries. An in-depth understanding of the dynamics of septic complications and the corresponding changes of the main markers of these diseases during treatment is especially relevant. The use of infusion therapy with solutions of donor albumin as an effective pathogenetic treatment is scientifically justified.展开更多
<strong>Background:</strong> Poor treatment of burns can lead to sepsis and even death. Especially promising is the use of the method of fluorescence spectroscopy for its diagnostics. <strong>The aim...<strong>Background:</strong> Poor treatment of burns can lead to sepsis and even death. Especially promising is the use of the method of fluorescence spectroscopy for its diagnostics. <strong>The aim of the research</strong> is to create a pathogenetic concept as the basis of a diagnostic and treatment model of purulent-inflammatory diseases and sepsis. <strong>Material and methods:</strong> The study lasted from 2001 to 2019 and included 4 stages. The experimental base of luminescent research at all stages was the luminescence laboratory of the Department of Experimental Physics of the Ivan Franko National University of Lviv. The study was performed by using optical monochromators MDR-2 and MDR-12. The excitation of the blood serum was performed with light with a wavelength of 280 nm, which corresponds to the glow region of human serum albumin. <strong>Results: </strong>The main indicators, used for the analysis, were the fluorescence intensity (<em>I</em><sub><em>F</em></sub>) and the position of the maximum fluorescence band (<em>λ</em><sub>max</sub>). The object of the study was samples of the blood serum of patients of the main and control groups. In the case of the presence of endogenous intoxication in the body, albumin binding centers are blocked by the products of bacterial metabolism and therefore such “pathological” albumin is unable to perform its functions, including transport and detoxification. <strong>Conclusions:</strong> The pathogenetic concept as the basis of the diagnostic and treating model of purulent-inflammatory diseases and sepsis was devised. In particular, the important role of albumin solution infusion in the treatment of the above-mentioned diseases was established.展开更多
<strong>Background: </strong>The main causes of mortality in patients with burn injury are the development of systemic inflammatory process, multiple organ failure and septic complications. <strong>T...<strong>Background: </strong>The main causes of mortality in patients with burn injury are the development of systemic inflammatory process, multiple organ failure and septic complications. <strong>The aim of the research:</strong> Improvement of diagnostic and therapeutic approaches to the detection and elimination of endogenous intoxication in patients with burn injury. <strong>Materials and methods:</strong> The main study group consisted of 19 patients and the comparison group—of 10 patients with burn injury. 92 blood serum (BS) samples of the patients of the main group were tested, using the method of fluorescence spectroscopy (MFS). The advanced therapeutic tactics were proposed for the patients of both groups. The control group consisted of 40 healthy individuals (donors). BS of these patients was also tested using MFS. <strong>Results: </strong>Patients with burn injury have endogenous intoxication in their blood. The effective concentration of albumin is reduced in patients with burn injury due to the blockage of albumin binding centers by bacterial metabolism products. Fluorescence spectra (FS) of BS in patients with burn trauma and donors were obtained and investigated. Based on MFS results, an improved treatment regimen using infusion of albumin solution was proposed. <strong>Conclusions:</strong> An improved technique for the management of patients with burn injury is based on the use of MFS for the diagnostic evaluation of endogenous intoxication in them. The idea of pathological changes in albumin molecules in patients with burn injury is pathogenetically substantiated by the successful use of infusion of albumin solution in these patients on the basis of the MFS.展开更多
In this study, the method of fluorescence spectroscopy was used to improve the diagnostics and prediction of sepsis, pyo-inflammatory diseases and postpartum endometritis. At the first stage of the study, the research...In this study, the method of fluorescence spectroscopy was used to improve the diagnostics and prediction of sepsis, pyo-inflammatory diseases and postpartum endometritis. At the first stage of the study, the researcher explored the fluorescence spectra of dilutions of serum with centrifuged and non-centrifuged bacterial culture (6-day crop sugar broth with Staphylococcus aureus), distilled water, 20% albumin and sugar broth. The focus was on the influence of treatment, including infusion therapy, on the fluorescence spectral characteristics of a patient’s serum. At the second stage, the method of fluorescence spectroscopy was used for the diagnosis of sepsis in vivo. At the third stage, the analyst scrutinized the peculiarities of pregnancy, childbirth, and the postpartum period (totally, 40 parameters) in patients with postpartum pyo-inflammatory diseases and in the control group.展开更多
Background: Currently, postpartum purulent-inflammatory diseases continue to be a prominent issue in medicine. As a result, numerous scientific publications were devoted to finding the solution to this issue. Primaril...Background: Currently, postpartum purulent-inflammatory diseases continue to be a prominent issue in medicine. As a result, numerous scientific publications were devoted to finding the solution to this issue. Primarily these solutions included the idea of optimisation of antibiotic-based disease prevention and therapies. However, the early diagnosis and prognosis of these pathologies were unfortunately overlooked. The Aim of the Study: To build a prognostic model of the development of postpartum purulent-inflammatory diseases. Material and Methods: The main focus of our research was establishment of methods of early diagnosis and prognosis of purulent-inflammatory diseases. The main cohort consisted of 170 women diagnosed with purulent-inflammatory diseases while the control cohort was made of 40 women with an uncomplicated course of pregnancy;patient’s blood serum was analysed using fluorescence spectroscopy. Additionally, we implied a variety of standardised algorithms used during clinical and laboratory examination of the patients with postpartum endometritis. Results: Fluorescence spectra were studied for 40 women of control group and 170 women of the main group. Based on the data obtained using fluorescence spectroscopy and data from clinical and laboratory examinations (extragenital pathology, gynecology-related diseases, risk of miscarriage, surgery, TORCH-infections, colpitis, labour duration > 12 hrs, labour anomalies, maximum blood serum fluorescence spectrum values, fluorescence spectrum ≤ 0.845, age, number of bed days, fetal distress), we have derived a prognostic model of the development of postpartum purulent-inflammatory diseases. Conclusion: As a result, we derived a prognostic model based on the main 13 factors, which contribute to development of postpartum purulent-inflammatory diseases. This model was determined correct with a probability of over 99% (р 2 = 174.74;df = 13).展开更多
<strong>Background: </strong>The significant improvement of immediate and long-term functional results of treating patients is the fundamental problem of modern medical science. A deep understanding of the...<strong>Background: </strong>The significant improvement of immediate and long-term functional results of treating patients is the fundamental problem of modern medical science. A deep understanding of the pathogenesis is the key point in creating the management strategy for patients with various diseases. Information about the mechanisms of origin and development of purulent-inflammatory diseases and sepsis is essential for finding effective ways to prevent and treat them. <strong>The aim of the research</strong> is to use the method of fluorescence spectroscopy in creating the pathogenetic diagnostic and treatment model for the prevention and treatment of purulent-inflammatory diseases and sepsis, modification of treatment tactics, search for new markers of purulent-septic diseases, as well as monitoring of patients during the treatment. <strong>Materials and methods: </strong>The proposed approach, along with standard diagnostic methods, was used to organize the treatment process of 100 patients with purulent-inflammatory diseases, including 15 patients with sepsis, 35 with acute inflammatory abdominal pathology, 20 patients with burn injury (main group) and 35 patients with burn injury (comparison group). <strong>Results:</strong> The behavior of spectral-fluorescent characteristics in their dynamics has been studied, and the new markers for assessing patients’ conditions have been proposed. Their effectiveness for the diagnosis of purulent-septic diseases has been proved, which advances the results of standard research methods by 24 - 48 hours.<strong> Conclusions:</strong> The proposed diagnostic and treatment approach is fundamentally important for diagnosis and monitoring during the treatment of patients with purulent-septic diseases. Particularly relevant is the proposal to modify the treatment process for these patients, associated with the use of infusion of donor albumin solutions.展开更多
文摘Background: The deep understanding of pathogenesis is a key moment in the formation of the modern strategy of modern medicine. We conducted the thorough analysis of the microscopic processes occurring in the bodies of patients with purulent-septic complications. The modified pathogenetic concept of the diagnostic and treatment model of diseases with septic complications is presented. The obtained information about the mechanisms of origin and development of these diseases is fundamentally important for finding the modern effective methods of treating patients. The aim of the research is to modify treatment tactics for patients with sepsis and burn injuries based on the modified pathogenetic concept using modern diagnostics, i.e. the method of fluorescence spectroscopy (MFS) and biomarkers. Materials and Methods: The proposed modified pathogenetic concept of the diagnostic and treatment model of diseases with purulent-septic complications along with standard methods was used successfully for effective treatment of 15 patients with sepsis and 25 with burn injuries. Results: 3 main scenarios of behaviour of spectral-fluorescence characteristics of patients with sepsis are illustrated. Spectral-fluorescence markers of sepsis were studied, which are informative 24 to 48 hours before the appearance of obvious clinical and laboratory signs of significant changes in the general somatic status of patients. Conclusions: The proposed diagnostic and therapeutic approach is new and fundamentally important for diagnostics and monitoring of the process of treatment of patients with purulent-septic diseases and burn injuries. An in-depth understanding of the dynamics of septic complications and the corresponding changes of the main markers of these diseases during treatment is especially relevant. The use of infusion therapy with solutions of donor albumin as an effective pathogenetic treatment is scientifically justified.
文摘<strong>Background:</strong> Poor treatment of burns can lead to sepsis and even death. Especially promising is the use of the method of fluorescence spectroscopy for its diagnostics. <strong>The aim of the research</strong> is to create a pathogenetic concept as the basis of a diagnostic and treatment model of purulent-inflammatory diseases and sepsis. <strong>Material and methods:</strong> The study lasted from 2001 to 2019 and included 4 stages. The experimental base of luminescent research at all stages was the luminescence laboratory of the Department of Experimental Physics of the Ivan Franko National University of Lviv. The study was performed by using optical monochromators MDR-2 and MDR-12. The excitation of the blood serum was performed with light with a wavelength of 280 nm, which corresponds to the glow region of human serum albumin. <strong>Results: </strong>The main indicators, used for the analysis, were the fluorescence intensity (<em>I</em><sub><em>F</em></sub>) and the position of the maximum fluorescence band (<em>λ</em><sub>max</sub>). The object of the study was samples of the blood serum of patients of the main and control groups. In the case of the presence of endogenous intoxication in the body, albumin binding centers are blocked by the products of bacterial metabolism and therefore such “pathological” albumin is unable to perform its functions, including transport and detoxification. <strong>Conclusions:</strong> The pathogenetic concept as the basis of the diagnostic and treating model of purulent-inflammatory diseases and sepsis was devised. In particular, the important role of albumin solution infusion in the treatment of the above-mentioned diseases was established.
文摘<strong>Background: </strong>The main causes of mortality in patients with burn injury are the development of systemic inflammatory process, multiple organ failure and septic complications. <strong>The aim of the research:</strong> Improvement of diagnostic and therapeutic approaches to the detection and elimination of endogenous intoxication in patients with burn injury. <strong>Materials and methods:</strong> The main study group consisted of 19 patients and the comparison group—of 10 patients with burn injury. 92 blood serum (BS) samples of the patients of the main group were tested, using the method of fluorescence spectroscopy (MFS). The advanced therapeutic tactics were proposed for the patients of both groups. The control group consisted of 40 healthy individuals (donors). BS of these patients was also tested using MFS. <strong>Results: </strong>Patients with burn injury have endogenous intoxication in their blood. The effective concentration of albumin is reduced in patients with burn injury due to the blockage of albumin binding centers by bacterial metabolism products. Fluorescence spectra (FS) of BS in patients with burn trauma and donors were obtained and investigated. Based on MFS results, an improved treatment regimen using infusion of albumin solution was proposed. <strong>Conclusions:</strong> An improved technique for the management of patients with burn injury is based on the use of MFS for the diagnostic evaluation of endogenous intoxication in them. The idea of pathological changes in albumin molecules in patients with burn injury is pathogenetically substantiated by the successful use of infusion of albumin solution in these patients on the basis of the MFS.
文摘In this study, the method of fluorescence spectroscopy was used to improve the diagnostics and prediction of sepsis, pyo-inflammatory diseases and postpartum endometritis. At the first stage of the study, the researcher explored the fluorescence spectra of dilutions of serum with centrifuged and non-centrifuged bacterial culture (6-day crop sugar broth with Staphylococcus aureus), distilled water, 20% albumin and sugar broth. The focus was on the influence of treatment, including infusion therapy, on the fluorescence spectral characteristics of a patient’s serum. At the second stage, the method of fluorescence spectroscopy was used for the diagnosis of sepsis in vivo. At the third stage, the analyst scrutinized the peculiarities of pregnancy, childbirth, and the postpartum period (totally, 40 parameters) in patients with postpartum pyo-inflammatory diseases and in the control group.
文摘Background: Currently, postpartum purulent-inflammatory diseases continue to be a prominent issue in medicine. As a result, numerous scientific publications were devoted to finding the solution to this issue. Primarily these solutions included the idea of optimisation of antibiotic-based disease prevention and therapies. However, the early diagnosis and prognosis of these pathologies were unfortunately overlooked. The Aim of the Study: To build a prognostic model of the development of postpartum purulent-inflammatory diseases. Material and Methods: The main focus of our research was establishment of methods of early diagnosis and prognosis of purulent-inflammatory diseases. The main cohort consisted of 170 women diagnosed with purulent-inflammatory diseases while the control cohort was made of 40 women with an uncomplicated course of pregnancy;patient’s blood serum was analysed using fluorescence spectroscopy. Additionally, we implied a variety of standardised algorithms used during clinical and laboratory examination of the patients with postpartum endometritis. Results: Fluorescence spectra were studied for 40 women of control group and 170 women of the main group. Based on the data obtained using fluorescence spectroscopy and data from clinical and laboratory examinations (extragenital pathology, gynecology-related diseases, risk of miscarriage, surgery, TORCH-infections, colpitis, labour duration > 12 hrs, labour anomalies, maximum blood serum fluorescence spectrum values, fluorescence spectrum ≤ 0.845, age, number of bed days, fetal distress), we have derived a prognostic model of the development of postpartum purulent-inflammatory diseases. Conclusion: As a result, we derived a prognostic model based on the main 13 factors, which contribute to development of postpartum purulent-inflammatory diseases. This model was determined correct with a probability of over 99% (р 2 = 174.74;df = 13).
文摘<strong>Background: </strong>The significant improvement of immediate and long-term functional results of treating patients is the fundamental problem of modern medical science. A deep understanding of the pathogenesis is the key point in creating the management strategy for patients with various diseases. Information about the mechanisms of origin and development of purulent-inflammatory diseases and sepsis is essential for finding effective ways to prevent and treat them. <strong>The aim of the research</strong> is to use the method of fluorescence spectroscopy in creating the pathogenetic diagnostic and treatment model for the prevention and treatment of purulent-inflammatory diseases and sepsis, modification of treatment tactics, search for new markers of purulent-septic diseases, as well as monitoring of patients during the treatment. <strong>Materials and methods: </strong>The proposed approach, along with standard diagnostic methods, was used to organize the treatment process of 100 patients with purulent-inflammatory diseases, including 15 patients with sepsis, 35 with acute inflammatory abdominal pathology, 20 patients with burn injury (main group) and 35 patients with burn injury (comparison group). <strong>Results:</strong> The behavior of spectral-fluorescent characteristics in their dynamics has been studied, and the new markers for assessing patients’ conditions have been proposed. Their effectiveness for the diagnosis of purulent-septic diseases has been proved, which advances the results of standard research methods by 24 - 48 hours.<strong> Conclusions:</strong> The proposed diagnostic and treatment approach is fundamentally important for diagnosis and monitoring during the treatment of patients with purulent-septic diseases. Particularly relevant is the proposal to modify the treatment process for these patients, associated with the use of infusion of donor albumin solutions.
