Electroacupuncture is beneficial for the recovery of spinal cord injury, but the underlying mechanism is unclear. The Rho/Rho-associated kinase（ROCK） signaling pathway regulates the actin cytoskeleton by controlling...Electroacupuncture is beneficial for the recovery of spinal cord injury, but the underlying mechanism is unclear. The Rho/Rho-associated kinase（ROCK） signaling pathway regulates the actin cytoskeleton by controlling the adhesive and migratory behaviors of cells that could inhibit neurite regrowth after neural injury and consequently hinder the recovery from spinal cord injury. Therefore, we hypothesized electroacupuncture could affect the Rho/ROCK signaling pathway to promote the recovery of spinal cord injury. In our experiments, the spinal cord injury in adult Sprague-Dawley rats was caused by an impact device. Those rats were subjected to electroacupuncture at Yaoyangguan（GV3）, Dazhui（GV14）, Zusanli（ST36） and Ciliao（BL32） and/or monosialoganglioside treatment. Behavioral scores revealed that the hindlimb motor functions improved with those treatments. Real-time quantitative polymerase chain reaction, fluorescence in situ hybridization and western blot assay showed that electroacupuncture suppressed the m RNA and protein expression of Rho-A and Rho-associated kinase Ⅱ（ROCKⅡ） of injured spinal cord. Although monosialoganglioside promoted the recovery of hindlimb motor function, monosialoganglioside did not affect the expression of Rho-A and ROCKⅡ. However, electroacupuncture combined with monosialoganglioside did not further improve the motor function or suppress the expression of Rho-A and ROCKⅡ. Our data suggested that the electroacupuncture could specifically inhibit the activation of the Rho/ROCK signaling pathway thus partially contributing to the repair of injured spinal cord. Monosialoganglioside could promote the motor function but did not suppress expression of Rho A and ROCKⅡ. There was no synergistic effect of electroacupuncture combined with monosialoganglioside.展开更多
We study the dynamics of single electron in an inhomogeneous cylindrical plasma channel during the direct acceleration by linearly polarized chirped laser pulse.By adjusting the parameters of the chirped laser pulse a...We study the dynamics of single electron in an inhomogeneous cylindrical plasma channel during the direct acceleration by linearly polarized chirped laser pulse.By adjusting the parameters of the chirped laser pulse and the plasma channel,we obtain the energy gain,trajectory,dephasing rate and unstable threshold of electron oscillation in the channel.The influences of the chirped factor and inhomogeneous plasma density distribution on the electron dynamics are discussed in depth.We find that the nonlinearly chirped laser pulse and the inhomogeneous plasma channel have strong coupled influence on the electron dynamics.The electron energy gain can be enhanced,the instability threshold of the electron oscillation can be lowered,and the acceleration length can be shortened by chirped laser,while the inhomogeneity of the plasma channel can reduce the amplitude of the chirped laser.展开更多
基金supported by the National Natural Science Foundation of China,No.81360562
文摘Electroacupuncture is beneficial for the recovery of spinal cord injury, but the underlying mechanism is unclear. The Rho/Rho-associated kinase（ROCK） signaling pathway regulates the actin cytoskeleton by controlling the adhesive and migratory behaviors of cells that could inhibit neurite regrowth after neural injury and consequently hinder the recovery from spinal cord injury. Therefore, we hypothesized electroacupuncture could affect the Rho/ROCK signaling pathway to promote the recovery of spinal cord injury. In our experiments, the spinal cord injury in adult Sprague-Dawley rats was caused by an impact device. Those rats were subjected to electroacupuncture at Yaoyangguan（GV3）, Dazhui（GV14）, Zusanli（ST36） and Ciliao（BL32） and/or monosialoganglioside treatment. Behavioral scores revealed that the hindlimb motor functions improved with those treatments. Real-time quantitative polymerase chain reaction, fluorescence in situ hybridization and western blot assay showed that electroacupuncture suppressed the m RNA and protein expression of Rho-A and Rho-associated kinase Ⅱ（ROCKⅡ） of injured spinal cord. Although monosialoganglioside promoted the recovery of hindlimb motor function, monosialoganglioside did not affect the expression of Rho-A and ROCKⅡ. However, electroacupuncture combined with monosialoganglioside did not further improve the motor function or suppress the expression of Rho-A and ROCKⅡ. Our data suggested that the electroacupuncture could specifically inhibit the activation of the Rho/ROCK signaling pathway thus partially contributing to the repair of injured spinal cord. Monosialoganglioside could promote the motor function but did not suppress expression of Rho A and ROCKⅡ. There was no synergistic effect of electroacupuncture combined with monosialoganglioside.
基金the National Natural Science Foundation of China(Grant Nos.11865014,11765017,11764039,11475027,11274255,and 11305132)the Natural Science Foundation of Gansu Province of China(Grant No.17JR5RA076)+2 种基金the Scientific Research Project of Gansu Higher Education of China(Grant No.2016A-005)the Natural Science Foundation of Education Department of Guizhou Province of China(Grant No.Qianjiaohe-KY-[2017]301)the Science and Technology Project of Guizhou Province of China(Grant No.Qiankehe-LH-[2017]7008).
文摘We study the dynamics of single electron in an inhomogeneous cylindrical plasma channel during the direct acceleration by linearly polarized chirped laser pulse.By adjusting the parameters of the chirped laser pulse and the plasma channel,we obtain the energy gain,trajectory,dephasing rate and unstable threshold of electron oscillation in the channel.The influences of the chirped factor and inhomogeneous plasma density distribution on the electron dynamics are discussed in depth.We find that the nonlinearly chirped laser pulse and the inhomogeneous plasma channel have strong coupled influence on the electron dynamics.The electron energy gain can be enhanced,the instability threshold of the electron oscillation can be lowered,and the acceleration length can be shortened by chirped laser,while the inhomogeneity of the plasma channel can reduce the amplitude of the chirped laser.
