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miR-223-3p靶向调控Rbpj转录因子对葡萄膜炎大鼠Th1和Th17细胞分化的影响 被引量:2
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作者 周梦贤 屈如意 +3 位作者 殷学伟 郭励劼 邱岩 郭大东 《国际眼科杂志》 CAS 北大核心 2022年第5期769-774,共6页
目的:探讨miR-223-3p对Notch信号通路转录因子Rbpj表达的调控作用以及对实验性自身免疫性葡萄膜炎(EAU)大鼠Th1、Th17细胞分化水平的影响。方法:用双荧光素酶表达报告系统探讨miR-223-3p对Rbpj基因表达的调控作用。将24只雌性Lewis大鼠... 目的:探讨miR-223-3p对Notch信号通路转录因子Rbpj表达的调控作用以及对实验性自身免疫性葡萄膜炎(EAU)大鼠Th1、Th17细胞分化水平的影响。方法:用双荧光素酶表达报告系统探讨miR-223-3p对Rbpj基因表达的调控作用。将24只雌性Lewis大鼠随机分为EAU模型组、正常对照组(NC组)和空白对照组(BC组),每组8只。EAU模型组注射含光感受器间维生素A结合蛋白(IRBP)、结核菌素和完全弗氏佐剂的乳糜液以诱导葡萄膜炎,BC组注射等体积的不含IRBP多肽的乳糜液,NC组注射等体积的无菌生理盐水。免疫后12d,无菌分离三组大鼠的脾脏、淋巴结和眼组织,实时荧光定量PCR(Q-PCR)检测miR-223-3p和Rbpj基因的表达水平;酶联免疫吸附测定(ELISA)方法检测Rbpj、IFN-γ、IL-17蛋白的表达水平;流式细胞仪检测三组大鼠不同组织中Th1和Th17细胞的表达水平。结果:双荧光素酶检测结果证实Rbpj是miR-223-3p调控的靶基因。免疫后12d,相比于NC组,EAU模型组大鼠脾脏、淋巴结和眼组织中miR-223-3p相对表达水平分别为0.33±0.29、0.11±0.12、0.18±0.11,均呈下调表达(均P<0.05);Rbpj mRNA水平均呈上调表达,分别为3.00±0.06、1.52±0.12、3.01±0.34(均P<0.05);BC组大鼠脾脏、淋巴结和眼组织中miR-223-3p和Rbpj mRNA水平与NC组相比均无差异(P>0.05)。ELISA检测结果发现,免疫后12d的EAU模型组大鼠各组织中Rbpj、IFN-γ、IL-17蛋白表达水平均明显高于NC组(均P<0.05),BC组大鼠各组织中Rbpj、IFN-γ、IL-17蛋白表达水平与NC组相比均无差异(P>0.05)。流式细胞仪检测结果显示,免疫后12d,EAU模型组各组织的Th1和Th17细胞比例均显著高于NC组(均P<0.05)。BC组与NC组各组织Th1和Th17细胞比例无明显变化(均P>0.05)。结论:miR-223-3p可负调控Notch信号通路转录因子Rbpj的表达。EAU大鼠中miR-223-3p的下调表达可升高Rbpj基因和蛋白的表达水平,促进Th1和Th17细胞的分化以及提高相关分子IFN-γ和IL-17的表达水平,进而影响葡萄膜炎的发生发展。 展开更多
关键词 miR-223-3p Rbpj 实验性自身免疫性葡萄膜炎 TH1 TH17 IFN-γ IL-17
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Metallographic structure,mechanical properties,and process parameter optimization of 5A06 joints formed by ultrasonic-assisted refill friction stir spot welding 被引量:4
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作者 Xin-bo Liu Feng-bin Qiao +1 位作者 li-jie guo Xiong-er Qiu 《International Journal of Minerals,Metallurgy and Materials》 SCIE EI CAS CSCD 2017年第2期164-170,共7页
Novel hybrid refill friction stir spot welding (RFSSW) assisted with ultrasonic oscillation was introduced to 5A06 aluminum alloy joints. The metallographic structure and mechanical properties of 5A06 aluminum alloy... Novel hybrid refill friction stir spot welding (RFSSW) assisted with ultrasonic oscillation was introduced to 5A06 aluminum alloy joints. The metallographic structure and mechanical properties of 5A06 aluminum alloy RFSSW joints formed without ultrasonic assistance and with lateral and longitudinal ultrasonic assistance were compared, and the ultrasonic-assisted RFSSW process parameters were opti- mized. The results show that compared with lateral ultrasonic oscillation, longitudinal ultrasonic oscillation strengthens the horizontal bond- ing ligament in the joint and has a stronger effect on the joint's shear strength. By contrast, lateral ultrasonic oscillation strengthens the ver- tical bonding ligament and is more effective in increasing the joint's tensile strength. The maximum shear strength of ultrasonic-assisted RFSSW 5A06 aluminum alloy joints is as high as 8761 N, and the maximum tensile strength is 3679 N when the joints are formed at a tool rotating speed of 2000 r/rain, a welding time of 3.5 s, a penetration depth of 0.2 mm, and an axial pressure of 11 kN. 展开更多
关键词 aluminum alloys friction stir welding ultrasonic applications METALLOGRAPHY mechanical properties parameter optimization
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Effect of curing time on the mesoscopic parameters of cemented paste backfill simulated using the particle flow code technique 被引量:3
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作者 Lang Liu Jie Xin +4 位作者 Chao Huan Yu-jiao Zhao Xiang Fan li-jie guo KI-IL Song 《International Journal of Minerals,Metallurgy and Materials》 SCIE EI CAS CSCD 2021年第4期590-602,共13页
Several special mechanical properties,such as dilatancy and compressibility,of cemented paste backfill(CPB)are controlled by its internal microstructure and evolution.The mesoscopic structure changes of CPB during the... Several special mechanical properties,such as dilatancy and compressibility,of cemented paste backfill(CPB)are controlled by its internal microstructure and evolution.The mesoscopic structure changes of CPB during the development process were investigated.On the basis of the scanning electron microscopy(SEM)and mechanical test results of CPB,the particle size information of CPB was extracted,and a two-dimensional particle flow code(PFC)model of CPB was established to analyze the evolution rule of mesoscopic parameters during CPB development.The embedded FISH language in PFC was used to develop a program for establishing a PFC model on the basis of the SEM results.The mesoscopic parameters of CPB samples at different curing times,such as coordination number(C_(n)),contact force chain,and rose diagram,were obtained by recording and loading and used to analyze the intrinsic relationship between mesoscopic parameter variations and macroscopic mechanical response during CPB development.It is of considerable significance to establish the physical model of CPB using the PFC to reveal the mesoscopic structure of CPB. 展开更多
关键词 cemented paste backfill particle flow code method mesoscopic parameters FABRIC
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A state-of-the-art review on delayed expansion of cemented paste backfill materials
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作者 Meng-Yuan Li li-jie guo +4 位作者 Yue Zhao Lei Zhang Kai Yang Xiao-Peng Peng Andrew Pan 《Rare Metals》 SCIE EI CAS CSCD 2024年第8期3475-3500,共26页
Utilizing delayed expansion cemented paste backfill materials(DECPB) in mine backfill operations offers an effective solution to the challenge of achieving proper contact between the in-situ cemented backfill mass and... Utilizing delayed expansion cemented paste backfill materials(DECPB) in mine backfill operations offers an effective solution to the challenge of achieving proper contact between the in-situ cemented backfill mass and the roof in mined-out areas(backfill-roof contact).This article presents recent progress in both theoretical and experimental research concerning DECPB.The types and mechanisms of expansive additives,including expansive agents,foaming agents,and expansive mineral materials relevant to DECPB,are explained,along with their impact on the expansive properties and mechanical characteristics of DECPB.The composition,material characteristics,preparation methods,and controlled release mechanisms of microcapsules are analyzed.Two methods for measuring the volume change rate of paste backfill slurries are discussed.Research indicates that an appropriate content of expansive additives not only enhances the expansive properties of cemented paste backfill materials but also contributes to improving their mechanical properties.Chemical foaming agents and bentonite are found to be more suitable for preparing DECPB compared to expansive agents.The delayed expansion of cemented paste backfill material is influenced by microcapsules,with their wall materials,preparation parameters,and the alkaline slurry environment affecting their controlled release mechanism.Developing and optimizing precise measurement devices and methods forfor the volume change rate of cemented paste backfill materials is essential requirement for studying the performance of DECPB.Combining delayed expansion technology with retarding techniques in cemented paste backfill materials can provide a reliable solution for achieving proper backfill-roof contact in the future. 展开更多
关键词 Paste backfill Delayed expansion Mining with backfill Expansive additives TAILINGS
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