BACKGROUND Gestational diabetes mellitus(GDM)women require prenatal care to minimize short-and long-term complications.The mechanism by which exercise during pregnancy affects organ development and whether glucose tra...BACKGROUND Gestational diabetes mellitus(GDM)women require prenatal care to minimize short-and long-term complications.The mechanism by which exercise during pregnancy affects organ development and whether glucose transporter(GLUT)1 plays a role in GDM offspring organ development remains unknown.AIM To determine the effect of exercise during pregnancy on the cardiac,hepatic and renal development of GDM mother’s offspring.METHODS Placenta samples were collected from humans and mice.GDM mouse models were created using streptozotocin along with a GDM with exercise group.The hearts,livers and kidneys of 3-and 8-week-old offspring were collected for body composition analysis and staining.The effects of high glucose levels and hypoxia were investigated using HTR8/SVneo.Transwell and wound-healing assays were performed to assess cell migration.Immunofluorescence accompanied with TUNEL and Ki67 staining was used to explore apoptosis and proliferation.RESULTS Exercise during pregnancy downregulated the GLUT1 and hypoxia inducible factor-1αexpression in placenta from individuals with GDM.Cobalt chloride induced hypoxia and high glucose levels also significantly decreased migration and apoptosis of HTR8/SVneo cells.In addition,exercise reduced inflammatory cell infiltration in the liver and decreased the tubular vacuolar area in the kidneys of offspring.CONCLUSION GDM affects the growth and development of organs in offspring.Exercise during pregnancy can reverse adverse effects of GDM on the development of the heart,liver,and kidney in offspring.展开更多
Diabetic foot ulcer(DFU)and poor wound healing are chronic complications in patients with diabetes.The increasing incidence of DFU has resulted in huge pressure worldwide.Diagnosing and treating this condition are the...Diabetic foot ulcer(DFU)and poor wound healing are chronic complications in patients with diabetes.The increasing incidence of DFU has resulted in huge pressure worldwide.Diagnosing and treating this condition are therefore of great importance to control morbidity and improve prognosis.Finding new markers with potential diagnostic and therapeutic utility in DFU has gathered increasing interest.Wound healing is a process divided into three stages:Inflammation,proliferation,and regeneration.Non-coding RNAs(ncRNAs),which are small protected molecules transcribed from the genome without protein translation function,have emerged as important regulators of diabetes complications.The deregulation of ncRNAs may be linked to accelerated DFU development and delayed wound healing.Moreover,ncRNAs can be used for therapeutic purposes in diabetic wound healing.Herein,we summarize the role of microRNAs,long ncRNAs,and circular RNAs in diverse stages of DFU wound healing and their potential use as novel therapeutic targets.展开更多
Irradiation tests of tungsten surface were performed with He and He/Ar plasma generated by microwave electron cyclotron resonance. Thickness loss was used as the erosion rate of tungsten surface under the plasma irrad...Irradiation tests of tungsten surface were performed with He and He/Ar plasma generated by microwave electron cyclotron resonance. Thickness loss was used as the erosion rate of tungsten surface under the plasma irradiation. The results revealed that the thickness loss increased linearly with negative bias. SEM images proved that the addition of Ar apparently increased the plasma erosion. The thickness loss increased sharply with the Ar fraction of Ar/He mixture when it was \20 %,where the increasing slope of thickness loss lowered down gradually.展开更多
AIM: To compare the efficacy and safety of photodynamic therapy(PDT) with overlapping multiple spots and single spot for treating circumscribed choroidal hemangioma. · METHODS: Twenty-two patients(22 eyes) with s...AIM: To compare the efficacy and safety of photodynamic therapy(PDT) with overlapping multiple spots and single spot for treating circumscribed choroidal hemangioma. · METHODS: Twenty-two patients(22 eyes) with symptomatic circumscribed choroidal hemangioma received PDT treatment. Fourteen patients received overlapping spots(two to three spots) PDT,whereas eight patients received single-spot PDT. Laser was used at 50J/cm2for 83s in the overlapping-spot group and 50J/cm2for 166s in the single-spot group. Clinical examination,funduscopy,fluorescein angiography,and ultrasonography were performed at baseline and after treatment. ·RESULTS: The mean follow-up time was 28.5 ±8.0 months in the overlapping-spot group and 27.0 ±5.0 months in the single-spot group. Nine patients(64.2%) had their vision improved over two lines on the Snellen chart,and five patients showed stable visual acuity in the overlapping-spot group. The mean thickness of tumor decreased from 2.7 ±0.8mm to 1.2±0.9mm,and the mean greatest tumor linear dimension decreased from 7.4 ± 1.5mm to 4.5 ±3.5mm after treatment. In the single-spot group,two patients(25%) had their vision improved over two lines on the Snellen chart,and six patients had unchanged stable vision. The mean tumor thickness in this group decreased from 2.5±0.7mm to 1.4±1.0mm,and the mean greatest tumor linear dimension decreased from 7.2±1.3mm to 4.7±3.6mm. No significant differences in visual improvement and tumor regression were found between the two groups. ·CONCLUSION:Overlapping-spotPDTunderappropriate treatment parameters and strategies is as effective andsafe as single-spot PDT for treating symptomatic circumscribed choroidal hemangioma. Improved or stabilized visual acuity was achieved as a result of tumor regression.展开更多
BACKGROUND Nipple eczema is the most common presentation of atopic dermatitis of the breast,which seriously influences breastfeeding of mothers.We here present a case of severe nipple eczema that started in puberty an...BACKGROUND Nipple eczema is the most common presentation of atopic dermatitis of the breast,which seriously influences breastfeeding of mothers.We here present a case of severe nipple eczema that started in puberty and received continuous care and interventions during pregnancy.The patient succeeded in breastfeeding after the interventions.CASE SUMMARY A 36-year-old woman at 16 wk of gestation(gestation 1 parturition 0),visited the breastfeeding consultation clinic,complaining of excessive nipple secretion,severe itching,and concerns about breastfeeding.She was diagnosed with severe nipple eczema.Health education,consultation with dermatologists,topical medication,psychological support,and postpartum care were carried out.Through continuous interventions,her nipple eczema significantly improved,lactating confidence enhanced,anxiety symptoms were reduced,and exclusive breastfeeding was achieved.CONCLUSION For lactating women with nipple eczema,breastfeeding consultants should play an important role in patient education and provide whole-process and individual guidance.展开更多
Non-alcoholic fatty liver disease(NAFLD)has become the world’s most common chronic liver disease and is considered one of the main causes of occult liver cirrhosis.To date,major guidelines in China and abroad have no...Non-alcoholic fatty liver disease(NAFLD)has become the world’s most common chronic liver disease and is considered one of the main causes of occult liver cirrhosis.To date,major guidelines in China and abroad have not recommended any drugs with precise efficacy and safety for NAFLD,especially for NAFLD-related liver fibrosis or cirrhosis,which lack effective drugs.Traditional Chinese medicine has significant curative effects and advantages in preventing and treating NAFLD.We retrospectively analyzed a case of liver cirrhosis due to NAFLD that was treated solely with traditional Chinese medicine for nearly one year at the Diagnosis and Treatment Center of Advantage Specific Diseases for Fatty Liver in our hospital to provide a reference for the prevention and treatment of non-alcoholic liver fibrosis and liver cirrhosis by traditional Chinese medicine.展开更多
以大肠杆菌菌毛蛋白CsgA组装形成的蛋白纤维为模板,引导不同数目的DNA四面体(tetrahedron DNA nanostructure,TDN)组装构建了蛋白-DNA亚微米复合结构. TDN经次氮基三乙酸(NTA)修饰后在Ni2+的螯合作用下与CsgA蛋白单体结合,利用CsgA的自...以大肠杆菌菌毛蛋白CsgA组装形成的蛋白纤维为模板,引导不同数目的DNA四面体(tetrahedron DNA nanostructure,TDN)组装构建了蛋白-DNA亚微米复合结构. TDN经次氮基三乙酸(NTA)修饰后在Ni2+的螯合作用下与CsgA蛋白单体结合,利用CsgA的自组装能力将TDN有序地排列在形成的蛋白纤维上.原子力显微镜(atomic force microscopy,AFM)成像结果表明,控制TDN与CsgA的浓度比为1:500,可以得到单个TDN与蛋白纤维的组装产物.将2个TDN通过杂交形成二聚体(dTDN)与CsgA蛋白进行组装,得到的亚微米复合结构保持了很好的直链形态,在蛋白纤维上连有3个dTDN结构的比例达44%.展开更多
Background： Preterm premature rapture of membrane （PPROM） can lead to serious consequences such as intrauterine infection, prolapse of the umbilical cord, and neonatal respiratory distress syndrome. Genital infecti...Background： Preterm premature rapture of membrane （PPROM） can lead to serious consequences such as intrauterine infection, prolapse of the umbilical cord, and neonatal respiratory distress syndrome. Genital infection is a very important risk which closely related with PPROM. The preliminary study only made qualitative research on genital infection, but there was no deep and clear judgment about the effects of pathogenic bacteria. This study was to analyze the association of in fections with PPROM in pregnant women in Shaanxi, China, and to establish Bayesian stepwise discriminant analysis to predict the incidence of PPROM. Methods： In training group, the 112 pregnant women with PPROM were enrolled in the case subgroup, and 108 normal pregnant women in the control subgroup using an unmatched case-control method. The sociodemographic characteristics of these participants were collected by face-to-face interviews. Vaginal excretions fiom each participant were sampled at 28 36-6 weeks of pregnancy using a sterile swab. DNA corresponding to Chlamrdia trachomalix （CT）, Ureaplasma urealyticwn （UU）, Candida albicans, group B streptococci （GBS）, herpes simplex virus- 1 （HSV-1）, and HSV-2 were detected in each participant by real-time polymerase chain reaction. A model of Bayesian discriminant analysis was established and then verified by a mull）center validation group that included 500 participants in the case subgroup and 5（10 participants in the control subgroup from five different hospitals in the Shaanxi province, respectively. Results： The sociological characteristics were not significantly different between the case and control subgroups in both training and validation groups （all P 〉 0.05）. In training group, the infection rates of UU （11.6% vs. 3.7%）, CT （17.0% vs. 5.6%）, and GBS （22.3% vs. 6.5%） showed statistically different between the case and control subgroups （all P 〈 0.05）, Iog-transfomacd quantification of UU, CE GBS, and HSV-2 showed statistically different between the case and control subgroups （P 〈 0.05）. All etiological agents were introduced into the Bayesian stepwise discriminant model showed that UU, CT, and GBS infections were the main contributors to PPROM, with coe｜＇ficients of 0.441,3.347, and 4.126, respectively. The accuracy rates of the Bayesian stepwise discriminant analysis between the case and control subgroup were 84.1% and 86.8% in the training and validation groups, respectively. Conclusions： This study established a Bayesian stepwise discriminant model to predict the incidence of PPROM. The UU, CT, and GBS infections were discriminant factors for PPROM according to a Bayesian stepwise discriminant analysis. This model could provide a new method for the early predicting of PPROM in pregnant women.展开更多
A record-breaking heavy rainfall swept across Suizhou of Hubei province in China on August 12,2021.It was characterized by suddenness and extremeness and caused severe socio-economic losses.To deepen the understanding...A record-breaking heavy rainfall swept across Suizhou of Hubei province in China on August 12,2021.It was characterized by suddenness and extremeness and caused severe socio-economic losses.To deepen the understanding of such an urban rainstorm and to improve the forecasting ability,this study revealed the dominant atmospheric circulation and moisture sources for this event.We performed a Lagrangian model FLEXPART to understand this event in terms of moisture sources and transport trajectories.Three key circulation systems affecting this extreme event were identified,including the western Pacific subtropical high(WPSH),the low-level southwesterly jet and an anticyclone over northern China.The low-level jet was lifted along the northern dry and cold air mass to form a front,resulting in the heavy rain.The moisture sources located on land contributed about 64%of the moisture.The development of the low-level southwesterly jet in southern China was strengthened near the time of the precipitation occurrence,providing the main moisture supply.Southern China(23°-32.5°N,98°-122°E)was the most important source region,which contributed the most to the precipitation(43.6%).The results highlight the prominent role of the terrestrial water cycle in this extreme precipitation event over Hubei.展开更多
基金Supported by Key R and D Program of Zhejiang Province,No.2022C03058Medical and Health Technology Program of Zhejiang Province,No.WKJ-ZJ-2324and 4+X Clinical Research Project of Women's Hospital,School of Medicine,Zhejiang University,No.ZDFY2022-4XB101.
文摘BACKGROUND Gestational diabetes mellitus(GDM)women require prenatal care to minimize short-and long-term complications.The mechanism by which exercise during pregnancy affects organ development and whether glucose transporter(GLUT)1 plays a role in GDM offspring organ development remains unknown.AIM To determine the effect of exercise during pregnancy on the cardiac,hepatic and renal development of GDM mother’s offspring.METHODS Placenta samples were collected from humans and mice.GDM mouse models were created using streptozotocin along with a GDM with exercise group.The hearts,livers and kidneys of 3-and 8-week-old offspring were collected for body composition analysis and staining.The effects of high glucose levels and hypoxia were investigated using HTR8/SVneo.Transwell and wound-healing assays were performed to assess cell migration.Immunofluorescence accompanied with TUNEL and Ki67 staining was used to explore apoptosis and proliferation.RESULTS Exercise during pregnancy downregulated the GLUT1 and hypoxia inducible factor-1αexpression in placenta from individuals with GDM.Cobalt chloride induced hypoxia and high glucose levels also significantly decreased migration and apoptosis of HTR8/SVneo cells.In addition,exercise reduced inflammatory cell infiltration in the liver and decreased the tubular vacuolar area in the kidneys of offspring.CONCLUSION GDM affects the growth and development of organs in offspring.Exercise during pregnancy can reverse adverse effects of GDM on the development of the heart,liver,and kidney in offspring.
基金Supported by the National Natural Science Foundation of China,No.81974234.
文摘Diabetic foot ulcer(DFU)and poor wound healing are chronic complications in patients with diabetes.The increasing incidence of DFU has resulted in huge pressure worldwide.Diagnosing and treating this condition are therefore of great importance to control morbidity and improve prognosis.Finding new markers with potential diagnostic and therapeutic utility in DFU has gathered increasing interest.Wound healing is a process divided into three stages:Inflammation,proliferation,and regeneration.Non-coding RNAs(ncRNAs),which are small protected molecules transcribed from the genome without protein translation function,have emerged as important regulators of diabetes complications.The deregulation of ncRNAs may be linked to accelerated DFU development and delayed wound healing.Moreover,ncRNAs can be used for therapeutic purposes in diabetic wound healing.Herein,we summarize the role of microRNAs,long ncRNAs,and circular RNAs in diverse stages of DFU wound healing and their potential use as novel therapeutic targets.
基金supported by the National Magnetic Confinement Fusion Program (Grant No. 2013GB109003)
文摘Irradiation tests of tungsten surface were performed with He and He/Ar plasma generated by microwave electron cyclotron resonance. Thickness loss was used as the erosion rate of tungsten surface under the plasma irradiation. The results revealed that the thickness loss increased linearly with negative bias. SEM images proved that the addition of Ar apparently increased the plasma erosion. The thickness loss increased sharply with the Ar fraction of Ar/He mixture when it was \20 %,where the increasing slope of thickness loss lowered down gradually.
基金Supported by the Zhejiang Medical Science Research Foundation of China(No.2009A108)Zhejiang Key Innovation Team Project of China(No.2011R09039-02)Zhejiang Key Laboratory Fund of China(No.2011E10006)
文摘AIM: To compare the efficacy and safety of photodynamic therapy(PDT) with overlapping multiple spots and single spot for treating circumscribed choroidal hemangioma. · METHODS: Twenty-two patients(22 eyes) with symptomatic circumscribed choroidal hemangioma received PDT treatment. Fourteen patients received overlapping spots(two to three spots) PDT,whereas eight patients received single-spot PDT. Laser was used at 50J/cm2for 83s in the overlapping-spot group and 50J/cm2for 166s in the single-spot group. Clinical examination,funduscopy,fluorescein angiography,and ultrasonography were performed at baseline and after treatment. ·RESULTS: The mean follow-up time was 28.5 ±8.0 months in the overlapping-spot group and 27.0 ±5.0 months in the single-spot group. Nine patients(64.2%) had their vision improved over two lines on the Snellen chart,and five patients showed stable visual acuity in the overlapping-spot group. The mean thickness of tumor decreased from 2.7 ±0.8mm to 1.2±0.9mm,and the mean greatest tumor linear dimension decreased from 7.4 ± 1.5mm to 4.5 ±3.5mm after treatment. In the single-spot group,two patients(25%) had their vision improved over two lines on the Snellen chart,and six patients had unchanged stable vision. The mean tumor thickness in this group decreased from 2.5±0.7mm to 1.4±1.0mm,and the mean greatest tumor linear dimension decreased from 7.2±1.3mm to 4.7±3.6mm. No significant differences in visual improvement and tumor regression were found between the two groups. ·CONCLUSION:Overlapping-spotPDTunderappropriate treatment parameters and strategies is as effective andsafe as single-spot PDT for treating symptomatic circumscribed choroidal hemangioma. Improved or stabilized visual acuity was achieved as a result of tumor regression.
基金Supported by the CAMS Innovation Fund for Medical Sciences(CIFMS),No.2021-I2M-1-023。
文摘BACKGROUND Nipple eczema is the most common presentation of atopic dermatitis of the breast,which seriously influences breastfeeding of mothers.We here present a case of severe nipple eczema that started in puberty and received continuous care and interventions during pregnancy.The patient succeeded in breastfeeding after the interventions.CASE SUMMARY A 36-year-old woman at 16 wk of gestation(gestation 1 parturition 0),visited the breastfeeding consultation clinic,complaining of excessive nipple secretion,severe itching,and concerns about breastfeeding.She was diagnosed with severe nipple eczema.Health education,consultation with dermatologists,topical medication,psychological support,and postpartum care were carried out.Through continuous interventions,her nipple eczema significantly improved,lactating confidence enhanced,anxiety symptoms were reduced,and exclusive breastfeeding was achieved.CONCLUSION For lactating women with nipple eczema,breastfeeding consultants should play an important role in patient education and provide whole-process and individual guidance.
基金The fifth batch of national training programs for clinical talents of traditional Chinese medicine(No.1[2022]of Chinese Medicine People’s Education Letter).Shaanxi Natural Science Basic Research Plan(2022JM-454).Scientific research project of Shaanxi Provincial Administration of Traditional Chinese Medicine(2021-PY-001)Science and Technology Project of Ankang Science and Technology Bureau(AK2020-SF-06,AK2021-SFZC-06).
文摘Non-alcoholic fatty liver disease(NAFLD)has become the world’s most common chronic liver disease and is considered one of the main causes of occult liver cirrhosis.To date,major guidelines in China and abroad have not recommended any drugs with precise efficacy and safety for NAFLD,especially for NAFLD-related liver fibrosis or cirrhosis,which lack effective drugs.Traditional Chinese medicine has significant curative effects and advantages in preventing and treating NAFLD.We retrospectively analyzed a case of liver cirrhosis due to NAFLD that was treated solely with traditional Chinese medicine for nearly one year at the Diagnosis and Treatment Center of Advantage Specific Diseases for Fatty Liver in our hospital to provide a reference for the prevention and treatment of non-alcoholic liver fibrosis and liver cirrhosis by traditional Chinese medicine.
文摘以大肠杆菌菌毛蛋白CsgA组装形成的蛋白纤维为模板,引导不同数目的DNA四面体(tetrahedron DNA nanostructure,TDN)组装构建了蛋白-DNA亚微米复合结构. TDN经次氮基三乙酸(NTA)修饰后在Ni2+的螯合作用下与CsgA蛋白单体结合,利用CsgA的自组装能力将TDN有序地排列在形成的蛋白纤维上.原子力显微镜(atomic force microscopy,AFM)成像结果表明,控制TDN与CsgA的浓度比为1:500,可以得到单个TDN与蛋白纤维的组装产物.将2个TDN通过杂交形成二聚体(dTDN)与CsgA蛋白进行组装,得到的亚微米复合结构保持了很好的直链形态,在蛋白纤维上连有3个dTDN结构的比例达44%.
文摘Background： Preterm premature rapture of membrane （PPROM） can lead to serious consequences such as intrauterine infection, prolapse of the umbilical cord, and neonatal respiratory distress syndrome. Genital infection is a very important risk which closely related with PPROM. The preliminary study only made qualitative research on genital infection, but there was no deep and clear judgment about the effects of pathogenic bacteria. This study was to analyze the association of in fections with PPROM in pregnant women in Shaanxi, China, and to establish Bayesian stepwise discriminant analysis to predict the incidence of PPROM. Methods： In training group, the 112 pregnant women with PPROM were enrolled in the case subgroup, and 108 normal pregnant women in the control subgroup using an unmatched case-control method. The sociodemographic characteristics of these participants were collected by face-to-face interviews. Vaginal excretions fiom each participant were sampled at 28 36-6 weeks of pregnancy using a sterile swab. DNA corresponding to Chlamrdia trachomalix （CT）, Ureaplasma urealyticwn （UU）, Candida albicans, group B streptococci （GBS）, herpes simplex virus- 1 （HSV-1）, and HSV-2 were detected in each participant by real-time polymerase chain reaction. A model of Bayesian discriminant analysis was established and then verified by a mull）center validation group that included 500 participants in the case subgroup and 5（10 participants in the control subgroup from five different hospitals in the Shaanxi province, respectively. Results： The sociological characteristics were not significantly different between the case and control subgroups in both training and validation groups （all P 〉 0.05）. In training group, the infection rates of UU （11.6% vs. 3.7%）, CT （17.0% vs. 5.6%）, and GBS （22.3% vs. 6.5%） showed statistically different between the case and control subgroups （all P 〈 0.05）, Iog-transfomacd quantification of UU, CE GBS, and HSV-2 showed statistically different between the case and control subgroups （P 〈 0.05）. All etiological agents were introduced into the Bayesian stepwise discriminant model showed that UU, CT, and GBS infections were the main contributors to PPROM, with coe｜＇ficients of 0.441,3.347, and 4.126, respectively. The accuracy rates of the Bayesian stepwise discriminant analysis between the case and control subgroup were 84.1% and 86.8% in the training and validation groups, respectively. Conclusions： This study established a Bayesian stepwise discriminant model to predict the incidence of PPROM. The UU, CT, and GBS infections were discriminant factors for PPROM according to a Bayesian stepwise discriminant analysis. This model could provide a new method for the early predicting of PPROM in pregnant women.
基金Second Tibetan Plateau Scientific Expedition and Research(STEP)program(2019QZKK0102)Chinese Academy of Sciences under grant from K.C.Wong Education Foundation。
文摘A record-breaking heavy rainfall swept across Suizhou of Hubei province in China on August 12,2021.It was characterized by suddenness and extremeness and caused severe socio-economic losses.To deepen the understanding of such an urban rainstorm and to improve the forecasting ability,this study revealed the dominant atmospheric circulation and moisture sources for this event.We performed a Lagrangian model FLEXPART to understand this event in terms of moisture sources and transport trajectories.Three key circulation systems affecting this extreme event were identified,including the western Pacific subtropical high(WPSH),the low-level southwesterly jet and an anticyclone over northern China.The low-level jet was lifted along the northern dry and cold air mass to form a front,resulting in the heavy rain.The moisture sources located on land contributed about 64%of the moisture.The development of the low-level southwesterly jet in southern China was strengthened near the time of the precipitation occurrence,providing the main moisture supply.Southern China(23°-32.5°N,98°-122°E)was the most important source region,which contributed the most to the precipitation(43.6%).The results highlight the prominent role of the terrestrial water cycle in this extreme precipitation event over Hubei.
