AIM： To investigate the effects on telomerase activity of transfection of human T-STAR gene full-length sense cDNA or partial antisense cDNA into human colon cancer cell line HCT-116.METHODS： mRNA and protein expres...AIM： To investigate the effects on telomerase activity of transfection of human T-STAR gene full-length sense cDNA or partial antisense cDNA into human colon cancer cell line HCT-116.METHODS： mRNA and protein expression levels of T-STAR gene were determined by RT-PCR and western blot, and telomerase activity was measured by PCR- ELISA, after transfection of T-STAR sense or antisense gene into HCT-116 cells with lipofectamine. RESULTS： T-STAR gene expression was enhanced or knocked down both at mRNA and protein levels, and telomerase activity was significantly increased or decreased. CONCLUSION： The T-STAR gene may participate in regulation of telomerase activity in human colon cancer HCT-116 cells in a parallel fashion.展开更多
Layered double hydroxides（LDHs） as a class of anionic clays have extensive applications due to their unique structures. Nowadays, the emphasis is laid on the development of LDH coatings for corrosion resistance and ...Layered double hydroxides（LDHs） as a class of anionic clays have extensive applications due to their unique structures. Nowadays, the emphasis is laid on the development of LDH coatings for corrosion resistance and medical applications. Thus, this review highlights synthetic methods of LDH coatings and LDH-based composite coatings on magnesium alloys. Special attention is focused on self-healing,biocompatible and self-cleaning LDH-based composite coatings on magnesium alloys.展开更多
Chiglitazar(Carfloglitazar)is a novel non-thiazolidinedione(TZD)structured peroxisome proliferatoractivated receptor(PPAR)pan-agonist that has shown promising effects on glycemic control and lipid regulation in patien...Chiglitazar(Carfloglitazar)is a novel non-thiazolidinedione(TZD)structured peroxisome proliferatoractivated receptor(PPAR)pan-agonist that has shown promising effects on glycemic control and lipid regulation in patients with type 2 diabetes in previous clinical studies.This randomized phase 3 trial aimed to compare the efficacy and safety of chiglitazar with placebo in patients with type 2 diabetes with insufficient glycemic control by strict diet and exercise alone.Eligible patients were randomly assigned to receive chiglitazar 32 mg(n=167),chiglitazar 48 mg(n=166),or placebo(n=202)once daily.The primary endpoint was the change in glycosylated hemoglobin A_(1c)(HbA_(1c))at week 24 with superiority of chiglitazar over placebo.The results showed that both chiglitazar 32 and 48 mg resulted in significant and clinically meaningful reductions in HbA_(1c),and placebo-adjusted estimated treatment differences at week 24 for chiglitazar 32 and 48 mg were-0.87%(95%confidential interval(CI):-1.10 to-0.65;P<0.0001)and-1.05%(95%CI:-1.29 to-0.81;P<0.0001),respectively.Secondary efficacy parameters including glycemic control,insulin sensitivity and triglyceride reduction were also significantly improved in the chiglitazar groups.The overall frequency of adverse events and study discontinuation attributable to adverse events were similar among the groups.Low incidences of mild edema and body weight gain were reported in the chiglitazar dose groups.The results from this phase 3 trial demonstrated that the PPAR pan-agonist chiglitazar possesses an overall good efficacy and safety profile in patients with type 2 diabetes inadequately controlled with lifestyle interventions,thereby providing adequate supporting evidence for using this PPAR pan-agonist as a treatment option for type 2 diabetes.展开更多
The magnesium aluminum and zinc aluminum layered double hydroxides intercalated with NO3^- （MgAl-NO3-LDH and ZnAl-NO3-LDH） were prepared by the coprecipitation method, and the magnesium aluminum and the zinc aluminu...The magnesium aluminum and zinc aluminum layered double hydroxides intercalated with NO3^- （MgAl-NO3-LDH and ZnAl-NO3-LDH） were prepared by the coprecipitation method, and the magnesium aluminum and the zinc aluminum layered double hydroxides intercalated with VO; （MgAl-VOx-LDH and ZnAl-VOx-LDH） were prepared by the anion-exchange method. Morphologies, microstructures and chemical compositions of LDHs were investigated by SEM, EDS, XRD, FTIR, Raman and TG analyses. The immersion tests were carried to determine the corrosion inhibition properties of MgAl-VOx-LDH and ZnAl-VOx-LDH on AZ31 Mg alloys. The results showed that ZnAl-VOx-LDH possesses the best anion-exchange and inhibition abilities. The influence of treatment parameters on microstructures of LDHs were discussed. Additionally, an inhibition mechanism for ZnAl-VOx-LDH on the AZ31 magnesium alloy was proposed and discussed.展开更多
BACKGROUND Adolescents and young adults might play a key role in the worldwide spread of Coronavirus Disease 2019(COVID-19)because they are more likely to be involved in overseas study,business,work,and travel.However...BACKGROUND Adolescents and young adults might play a key role in the worldwide spread of Coronavirus Disease 2019(COVID-19)because they are more likely to be involved in overseas study,business,work,and travel.However,the epidemiological and clinical characteristics remain unknown.展开更多
基金Supported by the National Natural Science Foundation of China,No. 30271442, No. 39980010
文摘AIM： To investigate the effects on telomerase activity of transfection of human T-STAR gene full-length sense cDNA or partial antisense cDNA into human colon cancer cell line HCT-116.METHODS： mRNA and protein expression levels of T-STAR gene were determined by RT-PCR and western blot, and telomerase activity was measured by PCR- ELISA, after transfection of T-STAR sense or antisense gene into HCT-116 cells with lipofectamine. RESULTS： T-STAR gene expression was enhanced or knocked down both at mRNA and protein levels, and telomerase activity was significantly increased or decreased. CONCLUSION： The T-STAR gene may participate in regulation of telomerase activity in human colon cancer HCT-116 cells in a parallel fashion.
基金supported by the National Natural Science Foundation of China (Grant Nos. 51601108, 51571134)the Natural Science Foundation of Shandong Province (Grant No. 2016ZRB01A62)the SDUST Research Fund (No. 2014TDJH104) and the Ministry-province jointly-constructed cultivation base for state key laboratory of Processing for non-ferrous metal and featured materials, "Guangxi Zhuang Autonomous Region"
文摘Layered double hydroxides（LDHs） as a class of anionic clays have extensive applications due to their unique structures. Nowadays, the emphasis is laid on the development of LDH coatings for corrosion resistance and medical applications. Thus, this review highlights synthetic methods of LDH coatings and LDH-based composite coatings on magnesium alloys. Special attention is focused on self-healing,biocompatible and self-cleaning LDH-based composite coatings on magnesium alloys.
基金grants from Chinese National and Provincial Major Project for New Drug Innovation(National:2008ZX09101-002 and 2013ZX09401301Provincial:2011A080501010)Shenzhen Municipal Major Project(2010-1746)。
文摘Chiglitazar(Carfloglitazar)is a novel non-thiazolidinedione(TZD)structured peroxisome proliferatoractivated receptor(PPAR)pan-agonist that has shown promising effects on glycemic control and lipid regulation in patients with type 2 diabetes in previous clinical studies.This randomized phase 3 trial aimed to compare the efficacy and safety of chiglitazar with placebo in patients with type 2 diabetes with insufficient glycemic control by strict diet and exercise alone.Eligible patients were randomly assigned to receive chiglitazar 32 mg(n=167),chiglitazar 48 mg(n=166),or placebo(n=202)once daily.The primary endpoint was the change in glycosylated hemoglobin A_(1c)(HbA_(1c))at week 24 with superiority of chiglitazar over placebo.The results showed that both chiglitazar 32 and 48 mg resulted in significant and clinically meaningful reductions in HbA_(1c),and placebo-adjusted estimated treatment differences at week 24 for chiglitazar 32 and 48 mg were-0.87%(95%confidential interval(CI):-1.10 to-0.65;P<0.0001)and-1.05%(95%CI:-1.29 to-0.81;P<0.0001),respectively.Secondary efficacy parameters including glycemic control,insulin sensitivity and triglyceride reduction were also significantly improved in the chiglitazar groups.The overall frequency of adverse events and study discontinuation attributable to adverse events were similar among the groups.Low incidences of mild edema and body weight gain were reported in the chiglitazar dose groups.The results from this phase 3 trial demonstrated that the PPAR pan-agonist chiglitazar possesses an overall good efficacy and safety profile in patients with type 2 diabetes inadequately controlled with lifestyle interventions,thereby providing adequate supporting evidence for using this PPAR pan-agonist as a treatment option for type 2 diabetes.
基金This work was supported by the National Natural Science Foundation of China （Grant Nos. 51601108, 51571134, 21676285）, the Natural Science Foundation of Shandong Province （Grant No. 2016ZRB01A62）, the SDUST Research Fund （No. 2014TDJH104）, and the Ministry-Province jointly-constructed cultivation base for the State Key Laboratory of Processing for Non-Ferrous Metal and Featured Materials, Guangxi Zhuang Autonomous Region.
文摘The magnesium aluminum and zinc aluminum layered double hydroxides intercalated with NO3^- （MgAl-NO3-LDH and ZnAl-NO3-LDH） were prepared by the coprecipitation method, and the magnesium aluminum and the zinc aluminum layered double hydroxides intercalated with VO; （MgAl-VOx-LDH and ZnAl-VOx-LDH） were prepared by the anion-exchange method. Morphologies, microstructures and chemical compositions of LDHs were investigated by SEM, EDS, XRD, FTIR, Raman and TG analyses. The immersion tests were carried to determine the corrosion inhibition properties of MgAl-VOx-LDH and ZnAl-VOx-LDH on AZ31 Mg alloys. The results showed that ZnAl-VOx-LDH possesses the best anion-exchange and inhibition abilities. The influence of treatment parameters on microstructures of LDHs were discussed. Additionally, an inhibition mechanism for ZnAl-VOx-LDH on the AZ31 magnesium alloy was proposed and discussed.
基金This work was supported by the Fundamental Research Funds for the Central Universities(No.2020CDJYGRH-YJ03).
文摘BACKGROUND Adolescents and young adults might play a key role in the worldwide spread of Coronavirus Disease 2019(COVID-19)because they are more likely to be involved in overseas study,business,work,and travel.However,the epidemiological and clinical characteristics remain unknown.
