生长阻滞和DNA损伤诱生蛋白45β(growth arrest and DNA damage 45β,GADD45β)参与多种细胞信号通路,在病毒感染过程中发挥重要作用,但在禽白血病病毒(avian leukosis virus,ALV)感染中研究较少。本研究中,用J亚群禽白血病病毒(ALV-J)...生长阻滞和DNA损伤诱生蛋白45β(growth arrest and DNA damage 45β,GADD45β)参与多种细胞信号通路,在病毒感染过程中发挥重要作用,但在禽白血病病毒(avian leukosis virus,ALV)感染中研究较少。本研究中,用J亚群禽白血病病毒(ALV-J)感染DF-1细胞,通过荧光定量PCR和Western blot检测GADD45β表达水平。此外,在过表达GADD45β和干扰GADD45β的情况下,通过Western blot、间接免疫荧光试验和ELISA检测GADD45β对ALV-J复制的影响。结果表明,ALV-J感染DF-1细胞能显著上调GADD45β表达水平(P<0.05)。过表达GADD45β后,ALV-J的蛋白表达水平显著降低(P<0.05),荧光信号强度也明显低于对照组。然而,干扰GADD45β后,ALV-J的复制水平显著上调(P<0.05),说明GADD45β可以抑制ALV-J病毒复制。本研究首次发现GADD45β具有抑制ALV-J病毒复制的功能,为抗ALV-J的研究提供了新的思路和理论基础。展开更多
We investigated the combined influence of joint inclination angle and joint continuity factor on deforma- tion behavior of jointed rock mass for gypsum specimens with a set of non-persistent open flaws in uni- axial c...We investigated the combined influence of joint inclination angle and joint continuity factor on deforma- tion behavior of jointed rock mass for gypsum specimens with a set of non-persistent open flaws in uni- axial compression. Complete axial stress-strain curves were classified into four types, i.e., single peak, softening after multi-peak yield platform, hardening after multi-peak yield platform and multi-peak dur- ing softening. Observation of crack evolution on the specimen surface reveals that the deformation behavior is correlated to the closure of pre-existing joint, development of fractures in rock matrix and teeth shearing of the shear plane. To investigate the brittleness of the specimens, the ratio of the residual strength to the maximum peak strength as well as the first and last peak strains were studied. At the same joint inclination angle, the ratios between residual strength and the maximum peak strength and the last peak strains increased while the first peak strain decreased with the increase of joint continuity factor. At the same joint continuity factor, the curves of the three brittleness parameters vs. joint inclina- tion angle can either be concave or convex single-oeak or wave-shaoed.展开更多
文摘生长阻滞和DNA损伤诱生蛋白45β(growth arrest and DNA damage 45β,GADD45β)参与多种细胞信号通路,在病毒感染过程中发挥重要作用,但在禽白血病病毒(avian leukosis virus,ALV)感染中研究较少。本研究中,用J亚群禽白血病病毒(ALV-J)感染DF-1细胞,通过荧光定量PCR和Western blot检测GADD45β表达水平。此外,在过表达GADD45β和干扰GADD45β的情况下,通过Western blot、间接免疫荧光试验和ELISA检测GADD45β对ALV-J复制的影响。结果表明,ALV-J感染DF-1细胞能显著上调GADD45β表达水平(P<0.05)。过表达GADD45β后,ALV-J的蛋白表达水平显著降低(P<0.05),荧光信号强度也明显低于对照组。然而,干扰GADD45β后,ALV-J的复制水平显著上调(P<0.05),说明GADD45β可以抑制ALV-J病毒复制。本研究首次发现GADD45β具有抑制ALV-J病毒复制的功能,为抗ALV-J的研究提供了新的思路和理论基础。
基金supported by the National Natural Science Foundation of China (No. 11102224)the Fundamental Research Funds for the Central Universities of China(No. 2009QL05)
文摘We investigated the combined influence of joint inclination angle and joint continuity factor on deforma- tion behavior of jointed rock mass for gypsum specimens with a set of non-persistent open flaws in uni- axial compression. Complete axial stress-strain curves were classified into four types, i.e., single peak, softening after multi-peak yield platform, hardening after multi-peak yield platform and multi-peak dur- ing softening. Observation of crack evolution on the specimen surface reveals that the deformation behavior is correlated to the closure of pre-existing joint, development of fractures in rock matrix and teeth shearing of the shear plane. To investigate the brittleness of the specimens, the ratio of the residual strength to the maximum peak strength as well as the first and last peak strains were studied. At the same joint inclination angle, the ratios between residual strength and the maximum peak strength and the last peak strains increased while the first peak strain decreased with the increase of joint continuity factor. At the same joint continuity factor, the curves of the three brittleness parameters vs. joint inclina- tion angle can either be concave or convex single-oeak or wave-shaoed.
