The safety and effectiveness of magnetic resonance-guided focused ultrasound thalamotomy has been broadly established and validated for the treatment of essential tremor.In 2018,the first magnetic resonance-guided foc...The safety and effectiveness of magnetic resonance-guided focused ultrasound thalamotomy has been broadly established and validated for the treatment of essential tremor.In 2018,the first magnetic resonance-guided focused ultrasound system in Chinese mainland was installed at the First Medical Center of the PLA General Hospital.This prospective,single center,open-label,single-arm study was part of a worldwide prospective multicenter clinical trial(ClinicalTrials.gov Identifier:NCT03253991)conducted to confirm the safety and efficacy of magnetic resonance-guided focused ultrasound for treating essential tremor in the local population.From 2019 to 2020,10 patients with medication refractory essential tremor were recruited into this open-label,single arm study.The treatment efficacy was determined using the Clinical Rating Scale for Tremor.Safety was evaluated according to the incidence and severity of adverse events.All of the subjects underwent a unilateral thalamotomy targeting the ventral intermediate nucleus.At the baseline assessment,the estimated marginal mean of the Clinical Rating Scale for Tremor total score was 58.3±3.6,and this improved after treatment to 23.1±6.4 at a 12-month follow-up assessment.A total of 50 adverse events were recorded,and 2 were defined as serious.The most common intraoperative adverse events were nausea and headache.The most frequent postoperative adverse events were paresthesia and equilibrium disorder.Most of the adverse events were mild and usually disappeared within a few days.Our findings suggest that magnetic resonance-guided focused ultrasound for the treatment of essential tremor is effective,with a good safety profile,for patients in Chinese mainland.展开更多
The mineralogical development and diagenetic sequence of lacustrine shales in the Chang 7 Member of the Yanchang Formation in the Ordos Basin are detailed studied.A model of their depositional system and a diagenetic ...The mineralogical development and diagenetic sequence of lacustrine shales in the Chang 7 Member of the Yanchang Formation in the Ordos Basin are detailed studied.A model of their depositional system and a diagenetic diagram are proposed in this study.Through detailed petrographic,mineralogical,and elemental analyses,four distinct shale types are identified:argillaceous shale,siliceous shale,calcareous shale,and carbonate,clay,and silt-bearing shale.The main diagenetic process in argillaceous shale is the transformation of illite to smectite,negatively impacting shale porosity.Siliceous shale undergoes carbonate cementation and quartz dissolution,contributing to increased porosity,particularly in mesopores.Calcareous shale experiences diagenesis characterised by carbonate formation and dissolution,with a prevalence of siderite.In carbonate,clay,and silt-bearing shale,the dissolution of K-feldspar contributes to illitization of kaolinite.Argillaceous shale,characterised by more clay minerals and lower mesopore volume,is identified as a potential hydrocarbon seal.Siliceous shale,with the highest pore volume and abundant inter-mineral pores,emerges as a promising shale oil reservoir.These findings contribute to a comprehensive understanding of shale properties,aiding in the prediction of shale oil exploration potential in the studied area.展开更多
The early stage evolution of local atomic structures in a multicomponent metallic glass during its crystallization process has been investigated via molecular dynamics simulation.It is found that the initial thermal s...The early stage evolution of local atomic structures in a multicomponent metallic glass during its crystallization process has been investigated via molecular dynamics simulation.It is found that the initial thermal stability and earliest stage evolution of the local atomic clusters show no strong correlation with their initial short-range orders,and this leads to an observation of a novel symmetry convergence phenomenon,which can be understood as an atomic structure manifestation of the ergodicity.Furthermore,in our system we have quantitatively proved that the crucial factor for the thermal stability against crystallization exhibited by the metallic glass is not the total amount of icosahedral clusters,but the degree of global connectivity among them.展开更多
BACKGROUND Spastic pelvic floor syndrome(SPFS)is a refractory pelvic floor disease characterized by abnormal(uncoordinated)contractions of the external anal sphincter and puborectalis muscle during defecation,resultin...BACKGROUND Spastic pelvic floor syndrome(SPFS)is a refractory pelvic floor disease characterized by abnormal(uncoordinated)contractions of the external anal sphincter and puborectalis muscle during defecation,resulting in rectal emptation and obstructive constipation.The clinical manifestations of SPFS are mainly characterized by difficult defecation,often accompanied by a sense of anal blockage and drooping.Manual defecation is usually needed during defecation.From physical examination,it is commonly observed that the patient's anal muscle tension is high,and it is difficult or even impossible to enter with his fingers.AIM To investigate the characteristics of anorectal pressure and botulinum toxin A injection combined with biofeedback in treating pelvic floor muscle spasm syndrome.METHODS Retrospective analysis of 50 patients diagnosed with pelvic floor spasm syndrome.All patients underwent pelvic floor surface electromyography assessment,anorectal dynamics examination,botulinum toxin type A injection 100 U intramuscular injection,and two cycles of biofeedback therapy.RESULTS After the botulinum toxin A injection combined with two cycles of biofeedback therapy,the patient's postoperative resting and systolic blood pressure were significantly lower than before surgery(P<0.05).Moreover,the electromyography index of the patients in the resting stage and post-resting stages was significantly lower than before surgery(P<0.05).CONCLUSION Botulinum toxin A injection combined with biofeedback can significantly reduce pelvic floor muscle tension in treating pelvic floor muscle spasm syndrome.Anorectal manometry is an effective method to evaluate the efficacy of treatment objectively.However,randomized controlled trials are needed.展开更多
BACKGROUND Epidemiological studies have revealed a correlation between Alzheimer’s disease(AD)and type 2 diabetes mellitus(T2D).Insulin resistance in the brain is a common feature in patients with T2D and AD.KAT7 is ...BACKGROUND Epidemiological studies have revealed a correlation between Alzheimer’s disease(AD)and type 2 diabetes mellitus(T2D).Insulin resistance in the brain is a common feature in patients with T2D and AD.KAT7 is a histone acetyltransferase that participates in the modulation of various genes.AIM To determine the effects of KAT7 on insulin patients with AD.METHODS APPswe/PS1-dE9 double-transgenic and db/db mice were used to mimic AD and diabetes,respectively.An in vitro model of AD was established by Aβstimulation.Insulin resistance was induced by chronic stimulation with high insulin levels.The expression of microtubule-associated protein 2(MAP2)was assessed using immunofluorescence.The protein levels of MAP2,Aβ,dual-specificity tyrosine phosphorylation-regulated kinase-1A(DYRK1A),IRS-1,p-AKT,total AKT,p-GSK3β,total GSK3β,DYRK1A,and KAT7 were measured via western blotting.Accumulation of reactive oxygen species(ROS),malondialdehyde(MDA),and SOD activity was measured to determine cellular oxidative stress.Flow cytometry and CCK-8 assay were performed to evaluate neuronal cell death and proliferation,respectively.Relative RNA levels of KAT7 and DYRK1A were examined using quantitative PCR.A chromatin immunoprecipitation assay was conducted to detect H3K14ac in DYRK1A.RESULTS KAT7 expression was suppressed in the AD mice.Overexpression of KAT7 decreased Aβaccumulation and MAP2 expression in AD brains.KAT7 overexpression decreased ROS and MDA levels,elevated SOD activity in brain tissues and neurons,and simultaneously suppressed neuronal apoptosis.KAT7 upregulated levels of p-AKT and p-GSK3βto alleviate insulin resistance,along with elevated expression of DYRK1A.KAT7 depletion suppressed DYRK1A expression and impaired H3K14ac of DYRK1A.HMGN1 overexpression recovered DYRK1A levels and reversed insulin resistance caused by KAT7 depletion.CONCLUSION We determined that KAT7 overexpression recovered insulin sensitivity in AD by recruiting HMGN1 to enhance DYRK1A acetylation.Our findings suggest that KAT7 is a novel and promising therapeutic target for the resistance in AD.展开更多
Prostate cancer is a malignant tumor with a high incidence in elderly men.In recent years,with the improvement of people’s living standards and the advancement of detection technology,the incidence of prostate cancer...Prostate cancer is a malignant tumor with a high incidence in elderly men.In recent years,with the improvement of people’s living standards and the advancement of detection technology,the incidence of prostate cancer has been increasing year by year.Castration-resistant prostate cancer(CRPC)is a highly challenging type of advanced prostate cancer treatment,which clinically shows resistance to hormonal deprivation therapy.The overall treatment efficacy of CRPC is currently poor and further relevant therapeutic studies are needed to improve patient survival and quality of life.Immunosuppressants can play a role in combating the immune system of tumors,and abiraterone has also achieved remarkable results in prostate cancer treatment.This study will investigate the possible clinical effects and safety of immunosuppressants combined with abiraterone in the treatment of metastatic CRPC.The population-based study will provide clinicians with more effective treatment options,as well as enhance the understanding of novel combination therapy strategies to be implemented in the future for such patients.展开更多
Objective： To evaluate the short-term clinical outcomes of intracranial germinoma patients treated with craniospinal irradiation （CSI） using helical tomotherapy （HT） system in our center. Methods： Twenty-three p...Objective： To evaluate the short-term clinical outcomes of intracranial germinoma patients treated with craniospinal irradiation （CSI） using helical tomotherapy （HT） system in our center. Methods： Twenty-three patients who were treated with CSI in our center from January 2008 to July 2012 were collected, with an average age of 20. M1 of the patients＇ CSI used the HT system. The total doses were 27-36 Gy/15-20 F （1.5-2 Gy per fraction）, and total local doses were 46-60 Gy/30-50 F （5 fractions per week）. M1 female patients for CSI were treated with left-right parallel-opposed field irradiation to protect their ovarian functions. Median follow-up time was 30.9 months （range, 5-67 months）. The SPSS19.0 software was used, and the overall survival （OS） was calculated using the Kaplan-Meier method. Results： Among 17 patients with assessable tumors, 9 cases （52.9%） were CR, 7 cases （41.2%） were PR, and 1 case （5.9%） was SD. Hematological toxicity was the severest side-effect occurred in the procedure of CSI. The level 1-4 acute leukopenia were 8.7%, 30.4%, 34.8% and 21.7% and the level 1-4 acute thrombopenia were 8.7%, 30.4%, 21.7% and 8.7%, respectively. Conclusions： For primary intracranial germinomas, HT can be used to implement CSI for simplifying radiotherapy procedures, improving radiotherapy accuracy, enhancing protection of peripheral organs at risk （ORA） and guaranteeing therapeutic effects. With the acceptable acute and long-term toxicity, CSI using HT in intracranial germinoma patients can be a safe and alternative mode.展开更多
Heterogenous distribution of crops,feed and livestock across China has halted the circulation of nutrients within the agricultural system and is responsible for massive nutrient losses[1,2].Generated livestock manure ...Heterogenous distribution of crops,feed and livestock across China has halted the circulation of nutrients within the agricultural system and is responsible for massive nutrient losses[1,2].Generated livestock manure exceeded optimal crop requirements in 30%and 50%of over 2300 studied counties when there was improved recycling of nitrogen(N)and phosphorus(P)in the food chain,repectively[2].Most of these counties are located in southern and coastal areas,whereas there is a deficit of livestock manure in northern and western China.Such heterogenous distribution of crop-livestock production led to 4.0 Tg manure N and 0.9 Tg manure P[2],which are economically impossible to recycle and will end up in the surrounding environment.In addition,about 40%of feed protein consumed by domestic livestock production relied on importation,putting China’s livestock production supply at high risk in the post pandemic world[3].Hence,China is facing the twin issues of too many manure nutrients but too little feed nutrients simultaneously.展开更多
In this work,a series of specimens was prepared by the casting method.Sharp cube-textured substrates were processed by heavy cold rolling and recrystallization annealing(i.e.,the rolling-assisted biaxially textured su...In this work,a series of specimens was prepared by the casting method.Sharp cube-textured substrates were processed by heavy cold rolling and recrystallization annealing(i.e.,the rolling-assisted biaxially textured substrates(RABi TS) method).Both the rolling and the recrystallization texture in the alloy tapes were investigated by X-ray diffraction and electron back-scatter diffraction,respectively.The results showed that a strong copper-type deformation texture was obtained in the heavy cold-rolled substrate.In addition,the recrystallization annealing process was found to be very important for the texture transition in the Cu–Ni alloy substrates.The cube texture content in the Cu60 Ni40 alloy substrates reached 99.7%(≤10°) after optimization of the cold-rolling procedure and the recrystallizing heat-treatment process,whereas the content of low-angle grain boundaries(from 2° to 10° misorientation) in the substrate reached 95.1%.展开更多
Erect milkvetch(Astragalus adsurgens)is a perennial legume forage crop with economic and ecological value in livestock grazing and soil-erosion control in arid and semiarid areas worldwide.Genomic information and mole...Erect milkvetch(Astragalus adsurgens)is a perennial legume forage crop with economic and ecological value in livestock grazing and soil-erosion control in arid and semiarid areas worldwide.Genomic information and molecular tools to support breeding and research in the species are limited.The objectives of this investigation were to map its genome using DNA markers and to identify quantitative trait loci(QTL)in the species.An F1 mapping population of 250 plants was developed from a cross between two parents with differing flowering-related traits.A high-density genetic linkage map containing 4821 markers on eight linkage groups(LGs)with a total genetic length of 1395 cM and a mean interval of 0.29 cM between adjacent markers was constructed with SLAF-seq technology.Comparative genomic analyses revealed the highest genome sequence similarity(8.71%)between erect milkvetch and Medicago truncatula,followed by Glycine max(7.65%),Cicer arietinum(7.53%),and Lupinus angustifolius(5.21%).A total of 64 significant QTL for flowering-related traits on six LGs were detected,accounting for 9.38 to 19.1%of the associated phenotype variation.Five and 48 key candidate genes for floret number and inflorescence length were identified based on the Glycyrrhiza uralensis genome.These candidate genes were involved in ubiquitination/degradation,pollen development,cell division,cytokinin biosynthetic process,and plant flowering.These findings shed light on the regulation of flowering traits in erect milkvetch and provide genomic resources for future molecular breeding of the crop.展开更多
Nanocrystalline TiO2 powders with high photocatalytic activity were prepared by one-step microemulsion-mediated hydrothermal method using tetrabutylorthotitanate (TiO(C4H9)4, TBOT) as precursor. The as-prepared TiO2 p...Nanocrystalline TiO2 powders with high photocatalytic activity were prepared by one-step microemulsion-mediated hydrothermal method using tetrabutylorthotitanate (TiO(C4H9)4, TBOT) as precursor. The as-prepared TiO2 powders were characterized by X-ray diffraction (XRD), transmission electron microscopy (TEM) and the Brunauer-Emmett-Teller (BET) specific surface area measurements. The effects of the oil/water ratio and hydrothermal temperature on the microstructures and photocatalytic activity of the TiO2 powders were investigated. The results suggest that increasing the oil/water emulsion ratio significantly decreased the particle size of the as-prepared TiO2 powders and improved the photocatalytic activity. With hydrothermal temperature increasing, the average crystallite size increased and the photocatalytic activities of TiO2 powders decreased.展开更多
Maintenance immunotherapy after concurrent chemoradiotherapy remains the standard therapeutic approach in patients with unresectable locally advanced non-small cell lung cancer(LA-NSCLC).The efficacy of pembrolizumab ...Maintenance immunotherapy after concurrent chemoradiotherapy remains the standard therapeutic approach in patients with unresectable locally advanced non-small cell lung cancer(LA-NSCLC).The efficacy of pembrolizumab without chemotherapy in stage IV NSCLC has incited interest in similar approaches for LA-NSCLC.Several recent investigations involving the synergistic potential of immunotherapy combined with radiotherapy(i RT)have generated encouraging results.This review discusses the existing studies and prospective directions of chemotherapy-free i RT strategies in unresectable LA-NSCLC.Although the initial findings of chemotherapy-free i RT strategies have shown promising efficacy,we must consider the methodologic limitations of current studies and the myriad of challenges that accompany the implementation of chemotherapy-free i RT.These challenges include determining the optimal dose and fractionation,precise target volume delineation,and identification of additional suitable patient cohorts.Furthermore,the feasibility of chemotherapy-free i RT as a novel treatment modality for select patients with LA-NSCLC is contingent upon validation through randomized phase III trials.展开更多
Background:To explore the role and mechanism of T cell imbalance in the process of immune thrombocytopenic purpura(ITP)and the efficacy of the Ningxue Shengban decoction in treating this disease.Methods:Passive immune...Background:To explore the role and mechanism of T cell imbalance in the process of immune thrombocytopenic purpura(ITP)and the efficacy of the Ningxue Shengban decoction in treating this disease.Methods:Passive immune ITP mouse model was established by injecting CD41 monoclonal antibody into BALB/c mice.The mice were divided into different groups for intervention and drug administration for 9 days.The therapeutic effects(blood cell count,bone marrow morphology)were observed.The changes in the number and proportion of regulatory T/Th17 cells in each group,as well as the expression of key transcription proteins and genes(Foxp3,RORγt)in mouse spleen,and the secretion of related inflammatory factors(interleukin-17,TGF-β,interleukin-21,interleukin-10)in serum were detected.Results:Ningxue Shengban decoction significantly increased the peripheral blood platelet count in ITP mice,improved bone marrow morphology,restored the imbalance of regulatory T/Th17 ratio,and exerted a positive regulatory effect on target proteins and downstream inflammatory factor secretion.Conclusion:Ningxue Shengban decoction may exert its therapeutic effect in treating ITP by regulating T cells and exerting immune regulatory function.展开更多
Insects are the most widely distributed and most abundant biological resources on earth,and are as precious a biological resource as plants and microorganisms.With the increasing population pressure and the serious sh...Insects are the most widely distributed and most abundant biological resources on earth,and are as precious a biological resource as plants and microorganisms.With the increasing population pressure and the serious shortage of food resources,insects as an ideal food substitute have gradually been paid more attention by scientists.In addition to being used as food and feed,the application value of insects in waste treatment,medicine and other aspects(such as ornamental and pollination)has also been widely recognized around the world.However,industrialization of insect resources still faces many challenges in terms of acceptability,safety,nutrition and productivity.We argue that insect mass production should be purpose-oriented and targeted cultivation.For example,insects used for food and feeding should be safe,tasty and nutritious.Insects used for waste disposal should maximize their survival rate and their conversion rate of the waste.For insects with medicinal significance,it is necessary to clarify their medicinal active ingredients and related pharmacological mechanism,and to cultivate insect strains with high content of active ingredients by proper induction.A goal-driven development strategy will help to avoid the risk of the insect industry technically and managerially.展开更多
An economic and feasible way to use black soldier fly larvae consists of treating livestock manure to convert it into high value-added protein feed.This study was aimed to investigate the effects of different pretreat...An economic and feasible way to use black soldier fly larvae consists of treating livestock manure to convert it into high value-added protein feed.This study was aimed to investigate the effects of different pretreatments on the physiochemical properties of pig manure and its potential in promoting the growth and nutrient accumulation of black soldier fly larvae(BSFL).Fresh pig manure was pretreated by aerobic fermentation(AE),anaerobic fermentation(AN)and high temperature anaerobic fermentation(HAN)for 2 or 5 d,and then inoculated with BSFL.Results indicate that EC value,DOC,DON,TN,NH_(4)^(+)-N and protein content were the highest in HAN pretreated manure.The individual body weight and protein content of BSFL fed with HAN pretreated manure increased by 20%and 30%,respectively,compared with those fed with AE pretreated manure.In terms of performance,BSFL fed with HAN pretreated manure showed the highest waste reduction,bioconversion rate,waste conversion efficiency and protein conversion efficiency.The conclusion of the present study may be helpful for further research on the conversion of organic waste by BSFL.展开更多
Objective To investigate the role of sea-level cerebral blood flow(CBF)in predicting acute mountain sickness(AMS)using three-dimensional pseudo-continuous arterial spin labeling(3D-pCASL).Methods Forty-eight healthy v...Objective To investigate the role of sea-level cerebral blood flow(CBF)in predicting acute mountain sickness(AMS)using three-dimensional pseudo-continuous arterial spin labeling(3D-pCASL).Methods Forty-eight healthy volunteers reached an altitude of 3,650 m by air after undergoing a head magnetic resonance imaging(MRI)including 3D-pCASL at sea level.The CBF values of the bilateral anterior cerebral artery(ACA),middle cerebral artery(MCA),posterior cerebral artery(PCA),and posterior inferior cerebellar artery(PICA)territories and the laterality index(LI)of CBF were compared between the AMS and non-AMS groups.Statistical analyses were performed to determine the relationship between CBF and AMS,and the predictive performance was assessed using receiver operating characteristic(ROC)curves.Results The mean cortical CBF in women(81.65±2.69 mL/100 g/min)was higher than that in men(74.35±2.12 mL/100 g/min)(P<0.05).In men,the cortical CBF values in the bilateral ACA,PCA,PICA,and right MCA were higher in patients with AMS than in those without.Cortical CBF in the right PCA best predicted AMS(AUC=0.818).In women,the LI of CBF in the ACA was different between the AMS and non-AMS groups and predicted AMS with an AUC of 0.753.Conclusion Although the mechanism and prediction of AMS are quite complicated,higher cortical CBF at sea level,especially the CBF of the posterior circulatory system,may be used for prediction in male volunteers using non-invasive 3D-pCASL.展开更多
Silk is widely used in the production of high-quality textiles.At the same time,the amount of silk textiles no longer in use and discarded is increasing,resulting in significant waste and pollution.This issue is of gr...Silk is widely used in the production of high-quality textiles.At the same time,the amount of silk textiles no longer in use and discarded is increasing,resulting in significant waste and pollution.This issue is of great concern in many countries where silk is used.Hydrogen peroxide as a naturally occurring compound is an important indicator of detection in both biology and the environment.This study aims to develop a composite fiber with hydrogen peroxide-sensing properties using discarded silk materials.To achieve this goal,firstly,polydopamine(PDA)was used to encapsulate the ZnFe_(2)O_(4) NPs to achieve the improvement of dispersion,and then regenerated silk fibroin(RSF)and PDA@ZnFe_(2)O_(4)/RSF hybrid fibers are prepared by wet spinning.Research has shown that PDA@ZnFe_(2)O_(4)/RSF demonstrates exceptional sensitivity,selectivity,and stability in detecting hydrogen peroxide,while maintaining high mechanical strength.Furthermore,the complete hybridization of PDA@ZnFe_(2)O_(4) with silk fibroin not only results in the combination of the durability of silk fibroin and PDA@ZnFe_(2)O_(4)’s rigidity,ensuring a reliable service life,but also makes PDA@ZnFe_(2)O_(4)/RSF exhibit excellent catalytic activity and biocompatibility.Therefore,the composite fiber exhibits exceptional mechanical properties and reliable hydrogen peroxide sensing capabilities,making it a promising material for biological and medical applications.展开更多
Accurate load forecasting forms a crucial foundation for implementing household demand response plans andoptimizing load scheduling. When dealing with short-term load data characterized by substantial fluctuations,a s...Accurate load forecasting forms a crucial foundation for implementing household demand response plans andoptimizing load scheduling. When dealing with short-term load data characterized by substantial fluctuations,a single prediction model is hard to capture temporal features effectively, resulting in diminished predictionaccuracy. In this study, a hybrid deep learning framework that integrates attention mechanism, convolution neuralnetwork (CNN), improved chaotic particle swarm optimization (ICPSO), and long short-term memory (LSTM), isproposed for short-term household load forecasting. Firstly, the CNN model is employed to extract features fromthe original data, enhancing the quality of data features. Subsequently, the moving average method is used for datapreprocessing, followed by the application of the LSTM network to predict the processed data. Moreover, the ICPSOalgorithm is introduced to optimize the parameters of LSTM, aimed at boosting the model’s running speed andaccuracy. Finally, the attention mechanism is employed to optimize the output value of LSTM, effectively addressinginformation loss in LSTM induced by lengthy sequences and further elevating prediction accuracy. According tothe numerical analysis, the accuracy and effectiveness of the proposed hybrid model have been verified. It canexplore data features adeptly, achieving superior prediction accuracy compared to other forecasting methods forthe household load exhibiting significant fluctuations across different seasons.展开更多
基金sponsored by Insightec Co.Ltd.(Israel)China National Clinical Research Center for Geriatrics,No.NCRCG-PLAGH-2019005 (to LP)
文摘The safety and effectiveness of magnetic resonance-guided focused ultrasound thalamotomy has been broadly established and validated for the treatment of essential tremor.In 2018,the first magnetic resonance-guided focused ultrasound system in Chinese mainland was installed at the First Medical Center of the PLA General Hospital.This prospective,single center,open-label,single-arm study was part of a worldwide prospective multicenter clinical trial(ClinicalTrials.gov Identifier:NCT03253991)conducted to confirm the safety and efficacy of magnetic resonance-guided focused ultrasound for treating essential tremor in the local population.From 2019 to 2020,10 patients with medication refractory essential tremor were recruited into this open-label,single arm study.The treatment efficacy was determined using the Clinical Rating Scale for Tremor.Safety was evaluated according to the incidence and severity of adverse events.All of the subjects underwent a unilateral thalamotomy targeting the ventral intermediate nucleus.At the baseline assessment,the estimated marginal mean of the Clinical Rating Scale for Tremor total score was 58.3±3.6,and this improved after treatment to 23.1±6.4 at a 12-month follow-up assessment.A total of 50 adverse events were recorded,and 2 were defined as serious.The most common intraoperative adverse events were nausea and headache.The most frequent postoperative adverse events were paresthesia and equilibrium disorder.Most of the adverse events were mild and usually disappeared within a few days.Our findings suggest that magnetic resonance-guided focused ultrasound for the treatment of essential tremor is effective,with a good safety profile,for patients in Chinese mainland.
基金founded by National Natural Science Foundation of China(grant Nos.:42072186 and 42090025)National Science and Technology Major Project,China(grant No.:2016ZX05046001)+3 种基金Science and Technology Research Project of Petro China Company Limited,China(grant No.:2021DJ1806)the fund support from China Scholarship Council(No.201806440002)the International Postdoctoral Exchange Fellowship Program,China(Talent-Introduction Program,No.270152)Lin Ma wishes to acknowledge the fund support from Natural Environment Research Council,United Kingdom(NE/R013527/1)。
文摘The mineralogical development and diagenetic sequence of lacustrine shales in the Chang 7 Member of the Yanchang Formation in the Ordos Basin are detailed studied.A model of their depositional system and a diagenetic diagram are proposed in this study.Through detailed petrographic,mineralogical,and elemental analyses,four distinct shale types are identified:argillaceous shale,siliceous shale,calcareous shale,and carbonate,clay,and silt-bearing shale.The main diagenetic process in argillaceous shale is the transformation of illite to smectite,negatively impacting shale porosity.Siliceous shale undergoes carbonate cementation and quartz dissolution,contributing to increased porosity,particularly in mesopores.Calcareous shale experiences diagenesis characterised by carbonate formation and dissolution,with a prevalence of siderite.In carbonate,clay,and silt-bearing shale,the dissolution of K-feldspar contributes to illitization of kaolinite.Argillaceous shale,characterised by more clay minerals and lower mesopore volume,is identified as a potential hydrocarbon seal.Siliceous shale,with the highest pore volume and abundant inter-mineral pores,emerges as a promising shale oil reservoir.These findings contribute to a comprehensive understanding of shale properties,aiding in the prediction of shale oil exploration potential in the studied area.
基金supported by the National Natural Science Foundation of China (Grant Nos. 52031016 and 11804027)the China Scholarship Council for financial support during part of this work
文摘The early stage evolution of local atomic structures in a multicomponent metallic glass during its crystallization process has been investigated via molecular dynamics simulation.It is found that the initial thermal stability and earliest stage evolution of the local atomic clusters show no strong correlation with their initial short-range orders,and this leads to an observation of a novel symmetry convergence phenomenon,which can be understood as an atomic structure manifestation of the ergodicity.Furthermore,in our system we have quantitatively proved that the crucial factor for the thermal stability against crystallization exhibited by the metallic glass is not the total amount of icosahedral clusters,but the degree of global connectivity among them.
文摘BACKGROUND Spastic pelvic floor syndrome(SPFS)is a refractory pelvic floor disease characterized by abnormal(uncoordinated)contractions of the external anal sphincter and puborectalis muscle during defecation,resulting in rectal emptation and obstructive constipation.The clinical manifestations of SPFS are mainly characterized by difficult defecation,often accompanied by a sense of anal blockage and drooping.Manual defecation is usually needed during defecation.From physical examination,it is commonly observed that the patient's anal muscle tension is high,and it is difficult or even impossible to enter with his fingers.AIM To investigate the characteristics of anorectal pressure and botulinum toxin A injection combined with biofeedback in treating pelvic floor muscle spasm syndrome.METHODS Retrospective analysis of 50 patients diagnosed with pelvic floor spasm syndrome.All patients underwent pelvic floor surface electromyography assessment,anorectal dynamics examination,botulinum toxin type A injection 100 U intramuscular injection,and two cycles of biofeedback therapy.RESULTS After the botulinum toxin A injection combined with two cycles of biofeedback therapy,the patient's postoperative resting and systolic blood pressure were significantly lower than before surgery(P<0.05).Moreover,the electromyography index of the patients in the resting stage and post-resting stages was significantly lower than before surgery(P<0.05).CONCLUSION Botulinum toxin A injection combined with biofeedback can significantly reduce pelvic floor muscle tension in treating pelvic floor muscle spasm syndrome.Anorectal manometry is an effective method to evaluate the efficacy of treatment objectively.However,randomized controlled trials are needed.
基金Supported by Natural Science Foundation of Shandong Province,No.ZR2020MH147National Natural Science Foundation of China,No.82002343.
文摘BACKGROUND Epidemiological studies have revealed a correlation between Alzheimer’s disease(AD)and type 2 diabetes mellitus(T2D).Insulin resistance in the brain is a common feature in patients with T2D and AD.KAT7 is a histone acetyltransferase that participates in the modulation of various genes.AIM To determine the effects of KAT7 on insulin patients with AD.METHODS APPswe/PS1-dE9 double-transgenic and db/db mice were used to mimic AD and diabetes,respectively.An in vitro model of AD was established by Aβstimulation.Insulin resistance was induced by chronic stimulation with high insulin levels.The expression of microtubule-associated protein 2(MAP2)was assessed using immunofluorescence.The protein levels of MAP2,Aβ,dual-specificity tyrosine phosphorylation-regulated kinase-1A(DYRK1A),IRS-1,p-AKT,total AKT,p-GSK3β,total GSK3β,DYRK1A,and KAT7 were measured via western blotting.Accumulation of reactive oxygen species(ROS),malondialdehyde(MDA),and SOD activity was measured to determine cellular oxidative stress.Flow cytometry and CCK-8 assay were performed to evaluate neuronal cell death and proliferation,respectively.Relative RNA levels of KAT7 and DYRK1A were examined using quantitative PCR.A chromatin immunoprecipitation assay was conducted to detect H3K14ac in DYRK1A.RESULTS KAT7 expression was suppressed in the AD mice.Overexpression of KAT7 decreased Aβaccumulation and MAP2 expression in AD brains.KAT7 overexpression decreased ROS and MDA levels,elevated SOD activity in brain tissues and neurons,and simultaneously suppressed neuronal apoptosis.KAT7 upregulated levels of p-AKT and p-GSK3βto alleviate insulin resistance,along with elevated expression of DYRK1A.KAT7 depletion suppressed DYRK1A expression and impaired H3K14ac of DYRK1A.HMGN1 overexpression recovered DYRK1A levels and reversed insulin resistance caused by KAT7 depletion.CONCLUSION We determined that KAT7 overexpression recovered insulin sensitivity in AD by recruiting HMGN1 to enhance DYRK1A acetylation.Our findings suggest that KAT7 is a novel and promising therapeutic target for the resistance in AD.
文摘Prostate cancer is a malignant tumor with a high incidence in elderly men.In recent years,with the improvement of people’s living standards and the advancement of detection technology,the incidence of prostate cancer has been increasing year by year.Castration-resistant prostate cancer(CRPC)is a highly challenging type of advanced prostate cancer treatment,which clinically shows resistance to hormonal deprivation therapy.The overall treatment efficacy of CRPC is currently poor and further relevant therapeutic studies are needed to improve patient survival and quality of life.Immunosuppressants can play a role in combating the immune system of tumors,and abiraterone has also achieved remarkable results in prostate cancer treatment.This study will investigate the possible clinical effects and safety of immunosuppressants combined with abiraterone in the treatment of metastatic CRPC.The population-based study will provide clinicians with more effective treatment options,as well as enhance the understanding of novel combination therapy strategies to be implemented in the future for such patients.
文摘Objective： To evaluate the short-term clinical outcomes of intracranial germinoma patients treated with craniospinal irradiation （CSI） using helical tomotherapy （HT） system in our center. Methods： Twenty-three patients who were treated with CSI in our center from January 2008 to July 2012 were collected, with an average age of 20. M1 of the patients＇ CSI used the HT system. The total doses were 27-36 Gy/15-20 F （1.5-2 Gy per fraction）, and total local doses were 46-60 Gy/30-50 F （5 fractions per week）. M1 female patients for CSI were treated with left-right parallel-opposed field irradiation to protect their ovarian functions. Median follow-up time was 30.9 months （range, 5-67 months）. The SPSS19.0 software was used, and the overall survival （OS） was calculated using the Kaplan-Meier method. Results： Among 17 patients with assessable tumors, 9 cases （52.9%） were CR, 7 cases （41.2%） were PR, and 1 case （5.9%） was SD. Hematological toxicity was the severest side-effect occurred in the procedure of CSI. The level 1-4 acute leukopenia were 8.7%, 30.4%, 34.8% and 21.7% and the level 1-4 acute thrombopenia were 8.7%, 30.4%, 21.7% and 8.7%, respectively. Conclusions： For primary intracranial germinomas, HT can be used to implement CSI for simplifying radiotherapy procedures, improving radiotherapy accuracy, enhancing protection of peripheral organs at risk （ORA） and guaranteeing therapeutic effects. With the acceptable acute and long-term toxicity, CSI using HT in intracranial germinoma patients can be a safe and alternative mode.
基金the National Key R&D Program of China(2016YFD0800106)the National Natural Science Foundation of China(31572210,31872403,71961137011)+5 种基金Key Research Program of Frontier Sciences-CAS(QYZDY-SSWSMC014)Key Laboratory of Agricultural Water Resources-CAS(ZD201802)the Key Research Program-CAS(KFJ-STS-ZDTP-053)Hebei Dairy Cattle Innovation Team of Modern Agroindustry Technology Research System,China(HBCT2018120206)the Youth Innovation Promotion Association,CAS(2019101)Outstanding Young Scientists Project of Natural Science Foundation of Hebei(C2019503054).
文摘Heterogenous distribution of crops,feed and livestock across China has halted the circulation of nutrients within the agricultural system and is responsible for massive nutrient losses[1,2].Generated livestock manure exceeded optimal crop requirements in 30%and 50%of over 2300 studied counties when there was improved recycling of nitrogen(N)and phosphorus(P)in the food chain,repectively[2].Most of these counties are located in southern and coastal areas,whereas there is a deficit of livestock manure in northern and western China.Such heterogenous distribution of crop-livestock production led to 4.0 Tg manure N and 0.9 Tg manure P[2],which are economically impossible to recycle and will end up in the surrounding environment.In addition,about 40%of feed protein consumed by domestic livestock production relied on importation,putting China’s livestock production supply at high risk in the post pandemic world[3].Hence,China is facing the twin issues of too many manure nutrients but too little feed nutrients simultaneously.
基金financially supported by the National Natural Science Foundation of China (No.51571002)the Beijing Natural Science Foundation (No.2172008)+4 种基金the Doctoral Program of Higher Education of Special Research Fund of China (No.20121103110012)the Beijing Municipal Natural Science Foundation B Type (No.KZ201310005003)the China Scholarship Councilthe Technology Program of Beijing CityBeijing University of Technology
文摘In this work,a series of specimens was prepared by the casting method.Sharp cube-textured substrates were processed by heavy cold rolling and recrystallization annealing(i.e.,the rolling-assisted biaxially textured substrates(RABi TS) method).Both the rolling and the recrystallization texture in the alloy tapes were investigated by X-ray diffraction and electron back-scatter diffraction,respectively.The results showed that a strong copper-type deformation texture was obtained in the heavy cold-rolled substrate.In addition,the recrystallization annealing process was found to be very important for the texture transition in the Cu–Ni alloy substrates.The cube texture content in the Cu60 Ni40 alloy substrates reached 99.7%(≤10°) after optimization of the cold-rolling procedure and the recrystallizing heat-treatment process,whereas the content of low-angle grain boundaries(from 2° to 10° misorientation) in the substrate reached 95.1%.
基金supported by the Chinese Universities Scientific Fund(2021RC001)the China Agriculture Research System(CARS34)the National Program for Forage Germplasm Conservation(2130135).
文摘Erect milkvetch(Astragalus adsurgens)is a perennial legume forage crop with economic and ecological value in livestock grazing and soil-erosion control in arid and semiarid areas worldwide.Genomic information and molecular tools to support breeding and research in the species are limited.The objectives of this investigation were to map its genome using DNA markers and to identify quantitative trait loci(QTL)in the species.An F1 mapping population of 250 plants was developed from a cross between two parents with differing flowering-related traits.A high-density genetic linkage map containing 4821 markers on eight linkage groups(LGs)with a total genetic length of 1395 cM and a mean interval of 0.29 cM between adjacent markers was constructed with SLAF-seq technology.Comparative genomic analyses revealed the highest genome sequence similarity(8.71%)between erect milkvetch and Medicago truncatula,followed by Glycine max(7.65%),Cicer arietinum(7.53%),and Lupinus angustifolius(5.21%).A total of 64 significant QTL for flowering-related traits on six LGs were detected,accounting for 9.38 to 19.1%of the associated phenotype variation.Five and 48 key candidate genes for floret number and inflorescence length were identified based on the Glycyrrhiza uralensis genome.These candidate genes were involved in ubiquitination/degradation,pollen development,cell division,cytokinin biosynthetic process,and plant flowering.These findings shed light on the regulation of flowering traits in erect milkvetch and provide genomic resources for future molecular breeding of the crop.
文摘Nanocrystalline TiO2 powders with high photocatalytic activity were prepared by one-step microemulsion-mediated hydrothermal method using tetrabutylorthotitanate (TiO(C4H9)4, TBOT) as precursor. The as-prepared TiO2 powders were characterized by X-ray diffraction (XRD), transmission electron microscopy (TEM) and the Brunauer-Emmett-Teller (BET) specific surface area measurements. The effects of the oil/water ratio and hydrothermal temperature on the microstructures and photocatalytic activity of the TiO2 powders were investigated. The results suggest that increasing the oil/water emulsion ratio significantly decreased the particle size of the as-prepared TiO2 powders and improved the photocatalytic activity. With hydrothermal temperature increasing, the average crystallite size increased and the photocatalytic activities of TiO2 powders decreased.
基金funded by the National Natural Science Foundation of China(Grant Nos.81972796,82272845,81972863,and 82030082)the Key Research and Development Program of Shandong(Major Science&Technology Innovation Project Grant No.2021SFGC0501)+1 种基金the CSCO-Haosen Foundation(Grant No.Y-HS202102-0089)the CSCO-Xinda Foundation(Grant No.Y-XD202001-0008)。
文摘Maintenance immunotherapy after concurrent chemoradiotherapy remains the standard therapeutic approach in patients with unresectable locally advanced non-small cell lung cancer(LA-NSCLC).The efficacy of pembrolizumab without chemotherapy in stage IV NSCLC has incited interest in similar approaches for LA-NSCLC.Several recent investigations involving the synergistic potential of immunotherapy combined with radiotherapy(i RT)have generated encouraging results.This review discusses the existing studies and prospective directions of chemotherapy-free i RT strategies in unresectable LA-NSCLC.Although the initial findings of chemotherapy-free i RT strategies have shown promising efficacy,we must consider the methodologic limitations of current studies and the myriad of challenges that accompany the implementation of chemotherapy-free i RT.These challenges include determining the optimal dose and fractionation,precise target volume delineation,and identification of additional suitable patient cohorts.Furthermore,the feasibility of chemotherapy-free i RT as a novel treatment modality for select patients with LA-NSCLC is contingent upon validation through randomized phase III trials.
基金Supported by National Natural Science Foundation of China:Mechanism study of Xijiao Dihuang combined Prescription in Treating Immune Thrombocytopenia Based on PD-1/PD-L1 Regulating Teff/Treg Immune Balance(No.8200152588)National Natural Science Foundation of China:Study on the mechanism of Yiqi Yangyin Qingre Huayu Recipe regulation of ITP platelet destruction and production through the imbalance of Th17/Treg and Notch pathway(No.81373847).
文摘Background:To explore the role and mechanism of T cell imbalance in the process of immune thrombocytopenic purpura(ITP)and the efficacy of the Ningxue Shengban decoction in treating this disease.Methods:Passive immune ITP mouse model was established by injecting CD41 monoclonal antibody into BALB/c mice.The mice were divided into different groups for intervention and drug administration for 9 days.The therapeutic effects(blood cell count,bone marrow morphology)were observed.The changes in the number and proportion of regulatory T/Th17 cells in each group,as well as the expression of key transcription proteins and genes(Foxp3,RORγt)in mouse spleen,and the secretion of related inflammatory factors(interleukin-17,TGF-β,interleukin-21,interleukin-10)in serum were detected.Results:Ningxue Shengban decoction significantly increased the peripheral blood platelet count in ITP mice,improved bone marrow morphology,restored the imbalance of regulatory T/Th17 ratio,and exerted a positive regulatory effect on target proteins and downstream inflammatory factor secretion.Conclusion:Ningxue Shengban decoction may exert its therapeutic effect in treating ITP by regulating T cells and exerting immune regulatory function.
基金supported by Key R&D Program of Hebei(21327315D,21373804D)the Special Research Assistant Funding Project of Chinese Academy of Sciences(E129ST15)+4 种基金the Strategic Priority Research Program of the Chinese Academy of Sciences(XDA28030302-4)Key Laboratory of Agricultural Water Resources-CAS(ZD201802)Strategic Priority Research Program of the Chinese Academy of Sciences(XDA28030302)Youth Innovation Promotion Association CAS(2021095)Hebei Dairy Cattle Innovation Team of Modern Agro-industry Technology Research System(HBCT2018120206).
文摘Insects are the most widely distributed and most abundant biological resources on earth,and are as precious a biological resource as plants and microorganisms.With the increasing population pressure and the serious shortage of food resources,insects as an ideal food substitute have gradually been paid more attention by scientists.In addition to being used as food and feed,the application value of insects in waste treatment,medicine and other aspects(such as ornamental and pollination)has also been widely recognized around the world.However,industrialization of insect resources still faces many challenges in terms of acceptability,safety,nutrition and productivity.We argue that insect mass production should be purpose-oriented and targeted cultivation.For example,insects used for food and feeding should be safe,tasty and nutritious.Insects used for waste disposal should maximize their survival rate and their conversion rate of the waste.For insects with medicinal significance,it is necessary to clarify their medicinal active ingredients and related pharmacological mechanism,and to cultivate insect strains with high content of active ingredients by proper induction.A goal-driven development strategy will help to avoid the risk of the insect industry technically and managerially.
基金supported by Key R&D Program of Hebei(21327315D,21373804D)the Special Research Assistant Funding Project of Chinese Academy of Sciences(E129ST15)+4 种基金the Strategic Priority Research Program of the Chinese Academy of Sciences(XDA28030302-4)Key Laboratory of Agricultural Water Resources-CAS(ZD201802)Strategic Priority Research Program of the Chinese Academy of Sciences(XDA28030302)Youth Innovation Promotion Association CAS(2021095)Hebei Dairy Cattle Innovation Team of Modern Agro-industry Technology Research System(HBCT2018120206).
文摘An economic and feasible way to use black soldier fly larvae consists of treating livestock manure to convert it into high value-added protein feed.This study was aimed to investigate the effects of different pretreatments on the physiochemical properties of pig manure and its potential in promoting the growth and nutrient accumulation of black soldier fly larvae(BSFL).Fresh pig manure was pretreated by aerobic fermentation(AE),anaerobic fermentation(AN)and high temperature anaerobic fermentation(HAN)for 2 or 5 d,and then inoculated with BSFL.Results indicate that EC value,DOC,DON,TN,NH_(4)^(+)-N and protein content were the highest in HAN pretreated manure.The individual body weight and protein content of BSFL fed with HAN pretreated manure increased by 20%and 30%,respectively,compared with those fed with AE pretreated manure.In terms of performance,BSFL fed with HAN pretreated manure showed the highest waste reduction,bioconversion rate,waste conversion efficiency and protein conversion efficiency.The conclusion of the present study may be helpful for further research on the conversion of organic waste by BSFL.
基金supported by the National Natural Science Foundation of China(No.81741115)Military Creative Project(No.16CXZ014)Military Healthcare Project(No.16BJZ11)。
文摘Objective To investigate the role of sea-level cerebral blood flow(CBF)in predicting acute mountain sickness(AMS)using three-dimensional pseudo-continuous arterial spin labeling(3D-pCASL).Methods Forty-eight healthy volunteers reached an altitude of 3,650 m by air after undergoing a head magnetic resonance imaging(MRI)including 3D-pCASL at sea level.The CBF values of the bilateral anterior cerebral artery(ACA),middle cerebral artery(MCA),posterior cerebral artery(PCA),and posterior inferior cerebellar artery(PICA)territories and the laterality index(LI)of CBF were compared between the AMS and non-AMS groups.Statistical analyses were performed to determine the relationship between CBF and AMS,and the predictive performance was assessed using receiver operating characteristic(ROC)curves.Results The mean cortical CBF in women(81.65±2.69 mL/100 g/min)was higher than that in men(74.35±2.12 mL/100 g/min)(P<0.05).In men,the cortical CBF values in the bilateral ACA,PCA,PICA,and right MCA were higher in patients with AMS than in those without.Cortical CBF in the right PCA best predicted AMS(AUC=0.818).In women,the LI of CBF in the ACA was different between the AMS and non-AMS groups and predicted AMS with an AUC of 0.753.Conclusion Although the mechanism and prediction of AMS are quite complicated,higher cortical CBF at sea level,especially the CBF of the posterior circulatory system,may be used for prediction in male volunteers using non-invasive 3D-pCASL.
基金supported by Guizhou Provincial Basic Research Program(Natural Science)(ZK[2024]574)Anshun University PhD Fund Project(No.asxybsjj202302)+1 种基金the National Synchrotron Radiation Laboratory(NSRL,Hefei,China)(No.2021-HLS-PT-004163)Shanghai Synchrotron Radiation Facility(SSRF,Shanghai,China)(No.2018-NFPS-PT-002700).
文摘Silk is widely used in the production of high-quality textiles.At the same time,the amount of silk textiles no longer in use and discarded is increasing,resulting in significant waste and pollution.This issue is of great concern in many countries where silk is used.Hydrogen peroxide as a naturally occurring compound is an important indicator of detection in both biology and the environment.This study aims to develop a composite fiber with hydrogen peroxide-sensing properties using discarded silk materials.To achieve this goal,firstly,polydopamine(PDA)was used to encapsulate the ZnFe_(2)O_(4) NPs to achieve the improvement of dispersion,and then regenerated silk fibroin(RSF)and PDA@ZnFe_(2)O_(4)/RSF hybrid fibers are prepared by wet spinning.Research has shown that PDA@ZnFe_(2)O_(4)/RSF demonstrates exceptional sensitivity,selectivity,and stability in detecting hydrogen peroxide,while maintaining high mechanical strength.Furthermore,the complete hybridization of PDA@ZnFe_(2)O_(4) with silk fibroin not only results in the combination of the durability of silk fibroin and PDA@ZnFe_(2)O_(4)’s rigidity,ensuring a reliable service life,but also makes PDA@ZnFe_(2)O_(4)/RSF exhibit excellent catalytic activity and biocompatibility.Therefore,the composite fiber exhibits exceptional mechanical properties and reliable hydrogen peroxide sensing capabilities,making it a promising material for biological and medical applications.
基金the Shanghai Rising-Star Program(No.22QA1403900)the National Natural Science Foundation of China(No.71804106)the Noncarbon Energy Conversion and Utilization Institute under the Shanghai Class IV Peak Disciplinary Development Program.
文摘Accurate load forecasting forms a crucial foundation for implementing household demand response plans andoptimizing load scheduling. When dealing with short-term load data characterized by substantial fluctuations,a single prediction model is hard to capture temporal features effectively, resulting in diminished predictionaccuracy. In this study, a hybrid deep learning framework that integrates attention mechanism, convolution neuralnetwork (CNN), improved chaotic particle swarm optimization (ICPSO), and long short-term memory (LSTM), isproposed for short-term household load forecasting. Firstly, the CNN model is employed to extract features fromthe original data, enhancing the quality of data features. Subsequently, the moving average method is used for datapreprocessing, followed by the application of the LSTM network to predict the processed data. Moreover, the ICPSOalgorithm is introduced to optimize the parameters of LSTM, aimed at boosting the model’s running speed andaccuracy. Finally, the attention mechanism is employed to optimize the output value of LSTM, effectively addressinginformation loss in LSTM induced by lengthy sequences and further elevating prediction accuracy. According tothe numerical analysis, the accuracy and effectiveness of the proposed hybrid model have been verified. It canexplore data features adeptly, achieving superior prediction accuracy compared to other forecasting methods forthe household load exhibiting significant fluctuations across different seasons.
