The characteristics of zinc finger of Kruppel homolog-1 (N/kr-hl) gene from brown planthopper and its effect on expression of FAMET and JHE were studied in the paper, in order to understand the regulation role of Nl...The characteristics of zinc finger of Kruppel homolog-1 (N/kr-hl) gene from brown planthopper and its effect on expression of FAMET and JHE were studied in the paper, in order to understand the regulation role of Nlkr-hl. The evolutionary tree of each zinc finger in functional area of Nlkr-hl was analyzed via MEGAS. 0 software, and expression status of FAMeT and JHE after interference of N/kr-hl was determined using qPCR technology. The results showed that the functional area of N/kr-hl mainly included eight zinc fingers, and the conservativeness of Znl and Zn8 was relatively lower. In the brown planthopper with N/b'-hl gene knockout, the expression levels of FAMeT and JHE increased. The paper provided the theoretical basis for understanding molecular mechanism of juvenile hormone signaling pathway and biological control of brown planthopper.展开更多
It has become a global problem to levy the standard value-added tax(VAT)on financial services.Due to the infeasibility of credit input VATs by invoice,it is infeasible to build a standard VAT system in this field.If t...It has become a global problem to levy the standard value-added tax(VAT)on financial services.Due to the infeasibility of credit input VATs by invoice,it is infeasible to build a standard VAT system in this field.If the government has to levy VAT on financial services,a practical choice is to adopt an account book method,which is to levy the special tax base through the account book using a single-rate.Meanwhile,it is not allowed to credit input VATs in this method.This kind of VAT is in effect a business tax.Due to the government’s authorized monopoly,there is no so-called over-taxation in China’s financial sector.As a result,the reform of“Replacing Business Tax with VAT”in finance should proceed without changing the original tax burden.After having conducted abundant research and statistical analyses,as for the VAT rate,9%is advised.The original way of levying taxes on the full amount of value is suggested to supplant with a new means of levying taxes on added value,with a slightly raised tax ratio,to reduce the tax base.展开更多
文摘The characteristics of zinc finger of Kruppel homolog-1 (N/kr-hl) gene from brown planthopper and its effect on expression of FAMET and JHE were studied in the paper, in order to understand the regulation role of Nlkr-hl. The evolutionary tree of each zinc finger in functional area of Nlkr-hl was analyzed via MEGAS. 0 software, and expression status of FAMeT and JHE after interference of N/kr-hl was determined using qPCR technology. The results showed that the functional area of N/kr-hl mainly included eight zinc fingers, and the conservativeness of Znl and Zn8 was relatively lower. In the brown planthopper with N/b'-hl gene knockout, the expression levels of FAMeT and JHE increased. The paper provided the theoretical basis for understanding molecular mechanism of juvenile hormone signaling pathway and biological control of brown planthopper.
文摘It has become a global problem to levy the standard value-added tax(VAT)on financial services.Due to the infeasibility of credit input VATs by invoice,it is infeasible to build a standard VAT system in this field.If the government has to levy VAT on financial services,a practical choice is to adopt an account book method,which is to levy the special tax base through the account book using a single-rate.Meanwhile,it is not allowed to credit input VATs in this method.This kind of VAT is in effect a business tax.Due to the government’s authorized monopoly,there is no so-called over-taxation in China’s financial sector.As a result,the reform of“Replacing Business Tax with VAT”in finance should proceed without changing the original tax burden.After having conducted abundant research and statistical analyses,as for the VAT rate,9%is advised.The original way of levying taxes on the full amount of value is suggested to supplant with a new means of levying taxes on added value,with a slightly raised tax ratio,to reduce the tax base.