2,4-dinitroanisole(DNAN)is a good replacement for 2,4,6-trinitrotoluene(TNT)in melt-cast explosives due to its superior insensitivity.With the increasing use of DNAN-based melt-cast explosives,the prediction of reacti...2,4-dinitroanisole(DNAN)is a good replacement for 2,4,6-trinitrotoluene(TNT)in melt-cast explosives due to its superior insensitivity.With the increasing use of DNAN-based melt-cast explosives,the prediction of reaction violence and hazard assessment of the explosives subjected to shock is of great significance.This study investigated the shock initiation characteristics for a DNAN-based melt-cast explosive,DHFA,using the one-dimensional Lagrangian apparatus.The embedded manganin gauges in the apparatus record the pressure histories at four Lagrangian positions and show that shock-todetonation transition in DHFA needs a high input shock pressure.The experimental data are analyzed to calibrate the Ignition and Growth model.The calibration is performed using an objective function based on both pressure history and the arrival time of shock.Good agreement between experimental and calculated pressure histories indicates the high accuracy of the calibrated parameters with the optimization method.展开更多
Dear Editor,Tuberculosis(TB)is an infectious disease which has been considered as a global public health emergency problem during the past 25y.The World Health Organization has estimated that approximately one-third o...Dear Editor,Tuberculosis(TB)is an infectious disease which has been considered as a global public health emergency problem during the past 25y.The World Health Organization has estimated that approximately one-third of the world’s population are infected by TB.However,only 10%of patients with TB is symptomatic.TB can affect multiple organs throughout the body.Eighty percent of those infected are pulmonary TB.Ocular TB can occur with or without lung or other systemic TB.It is a rare extrapulmonary form of the disease and occupied about 3.5%–5%of all of the TB.It should not be disregarded as its potential influence on vision loss.The intraocular and/or extraocular structures could be impacted by ocular TB[1].Uvea was the most common affected structure[2].展开更多
Background:Wernicke encephalopathy(WE)is an acute neurological disease resulting from vitamin B1 deficiency,and there are only very few case reports of WE after liver transplantation.The present study aimed to investi...Background:Wernicke encephalopathy(WE)is an acute neurological disease resulting from vitamin B1 deficiency,and there are only very few case reports of WE after liver transplantation.The present study aimed to investigate the clinical characteristics,etiology,magnetic resonance imaging(MRI)features,treatment and prognosis of patients with WE after liver transplantation.Methods:Twenty-three patients with WE after liver transplantation from the First Affiliated Hospital,Zhejiang University School of Medicine and Jiangxi Provincial People’s Hospital between January 2011 and December 2021 were retrospectively analyzed.Results:Among the 23 patients diagnosed with WE after liver transplantation,6(26%)had a classic triad of impaired consciousness,oculomotor palsy and ataxia,and 17(74%)had two features.The misdiagno-sis rate was 65%.After treatment with high-dose vitamin B1,19(83%)patients showed improvement,whereas 4(17%)showed no improvement,including 3 with residual short-term memory impairments and 1 with residual spatial and temporal disorientation and ataxia.Conclusions:The misdiagnosis rate is high in the early stage of WE,and the prognosis is closely asso-ciated with whether WE is diagnosed early and treated timely.High-dose glucose or glucocorticoids can trigger WE and cannot be administered before vitamin B1 treatment.Vitamin B1 is suggested to be used as a prophylactic treatment for patients with WE after liver transplantation.展开更多
Uncertainty is an important characteristic of scheduling model for scale farm machinery operation organizing. Practice shows that scheduling model without considering uncertainties is nearly useless. Uncertain influen...Uncertainty is an important characteristic of scheduling model for scale farm machinery operation organizing. Practice shows that scheduling model without considering uncertainties is nearly useless. Uncertain influence factors arisen from natural environment, society and economy, market and supply, and customer and behavior, exist widely, emerge frequently, and affect production deeply. Uncertainties interfere with the allocation of productive factors on temporal and spatial dimensions for farm machinery operation scheduling and management. Questionnaire for farm machinery organizations was designed and finished in 2014. Both occurrence frequency and influence degree for each factor were quantified. Four influence factors including operation location change, weather mutation, parts supply delay, and operation skill defects appear in both list of high occurrence and deep influence. Then results of questionnaire and results of specific investigation were used to study temporal and spatial scheduling model and system for farm machinery management. Three case studies are introduced. The first case is about the uncertainty and countermeasure of forage harvesters scheduling and monitoring for a professional forage plantation company. The second case is about the uncertainty and counter measure of cotton-picker scheduling and monitoring for a professional cotton picking company. And the third case is about the uncertainty and countermeasure of social service management for a professional cooperative. The cases show that the research has strong pertinence to deal with uncertainties and can improve management efficiency of farm machinery operation.展开更多
The construction of a stable-membrane tracker has significant implications for the visualization of the membrane in live cells.However,most current plasma trackers are not suitable for tracking plasma membranes for a ...The construction of a stable-membrane tracker has significant implications for the visualization of the membrane in live cells.However,most current plasma trackers are not suitable for tracking plasma membranes for a long time due to their limited retention time.Herein,Mem580-F-Sulfo is designed to target and anchor cell membranes and therefore track cell membranes for a longer time.This tracker is composed of a lipophilic boron-dipyrromethene(BODIPY)derivative and a hydrophilic zwitterion to form an amphiphilic structure,which enables its targeting ability toward cell membranes.Moreover,a reactive ester group is included to bind with proteins through covalent bonds in cell membranes nonspecifically,which extends retention time in cell membranes.Mem580-F-Sulfo shows intense brightness(94600),with a high molar absorption coefficient of up to about 100000 L·mol^(-1)·cm^(-1)and a fluorescence quantum yield of up to 0.97.It shows fast cell membrane targeting ability and long retention up to 90 min.In brief,this work has not only developed a tracker with good cell membrane targetability but also provided a new strategy for improving the targeting stability of cell membranes.展开更多
It is a major challenge for the airframe-inlet design of modern combat aircrafts,as the flow and electromagnetic wave propagation in the inlet of stealth aircraft are very complex.In this study,an aerodynamic/stealth ...It is a major challenge for the airframe-inlet design of modern combat aircrafts,as the flow and electromagnetic wave propagation in the inlet of stealth aircraft are very complex.In this study,an aerodynamic/stealth optimization design method for an S-duct inlet is proposed.The upwind scheme is introduced to the aerodynamic adjoint equation to resolve the shock wave and flow separation.The multilevel fast multipole algorithm(MLFMA)is utilized for the stealth adjoint equation.A dorsal S-duct inlet of flying wing layout is optimized to improve the aerodynamic and stealth characteristics.Both the aerodynamic and stealth characteristics of the inlet are effectively improved.Finally,the optimization results are analyzed,and it shows that the main contradiction between aerodynamic characteristics and stealth characteristics is the centerline and crosssectional area.The S-duct is smoothed,and the cross-sectional area is increased to improve the aerodynamic characteristics,while it is completely opposite for the stealth design.The radar cross section(RCS)is reduced by phase cancelation for low frequency conditions.The method is suitable for the aerodynamic/stealth design of the aircraft airframe-inlet system.展开更多
BACKGROUND Cholesterol stones affect a certain subpopulation of children.Concerns have been raised on the impact of gallbladder surgery on the growth of children and adolescents.AIM To study the population characteris...BACKGROUND Cholesterol stones affect a certain subpopulation of children.Concerns have been raised on the impact of gallbladder surgery on the growth of children and adolescents.AIM To study the population characteristics,clinical features,treatment,and prognosis of gallstones in children.METHODS The clinical data of 44 children with gallstones admitted to The First Affiliated Hospital of Naval Medical University from August 2009 to August 2021 were collected,the children were followed up by telephone to monitor their prognoses.The follow-up ended in August 2023.The shortest follow-up time was 2 years and 6 months,whereas the longest was 13 years and 11 months.The population characteristics,general clinical characteristics,and treatments were retrospectively analyzed.The children were divided according to whether they underwent surgical gallbladder removal into an operation group(n=28)and a non-operation group(n=16),The effects of surgical gallbladder resection on the growth and development of children were analyzed.RESULTS The male–female ratio in the population was 6:5 and 84.09%of the children had onset in adolescence.Furthermore,29.55%of the children were overweight or obese.The study identified 26 cases with metabolic abnormalities,9 with hemolytic anemia,and 4 with choledochal cyst.Of the population,68.18%had recurrent symptomatic cholecystolithiasis.Surgical treatment accounted for 63.64%,with laparoscopic cholecystectomy accounting for 71.43%of surgical treatment.No significant differences were observed in symptoms and complications between the surgery and non-surgery groups.Furthermore,no significant differences were found between the two groups in the attainment of genetic height target and the rightward shift of height curve during follow-up.CONCLUSION The sex characteristics of gallstones in children were not observed.Most gallstones occurred in adolescents and rarely in young children.A considerable proportion of children have inborn causes,which are often concurrent with metabolic abnormalities and hemolytic anemia.Most children had recurrent symptomatic gallstones.Surgical treatment,especially laparoscopic cholecystectomy,is still the main treatment for gallstones in children.Surgical treatment did not affect the growth and development of children who underwent gallstone removal.展开更多
目的探讨全程多学科营养模式在老年患者髋部手术中的应用效果。方法选取2021年10月至2023年12月于温州市中心医院行髋部手术的老年患者90例,根据随机数字表法将其分为对照组和研究组,每组各45例。对照组患者应用饮食管理模式,研究组患...目的探讨全程多学科营养模式在老年患者髋部手术中的应用效果。方法选取2021年10月至2023年12月于温州市中心医院行髋部手术的老年患者90例,根据随机数字表法将其分为对照组和研究组,每组各45例。对照组患者应用饮食管理模式,研究组患者应用全程多学科营养模式。比较两组患者的恢复情况、营养状况、生活质量、髋关节功能和满意度。结果研究组患者的住院时间、骨折愈合时间均显著短于对照组(P<0.05)。干预后,两组患者的前白蛋白(prealbumin,PA)、白蛋白(albumin,Alb)、血红蛋白(hemoglobin,Hb)、健康调查量表36(36-item short form health survey,SF-36)各维度评分、Harris髋关节评分均显著高于本组干预前(P<0.05),且研究组患者的PA、Alb、Hb、SF-36各维度评分、Harris髋关节评分均显著高于对照组(P<0.05)。研究组患者的满意度显著高于对照组(χ^(2)=5.075,P=0.024)。结论老年患者髋部手术中应用全程多学科营养模式可改善其营养状态,提升生活质量,增强髋关节功能,有利于术后恢复。展开更多
In VTI media,the conventional inversion methods based on the existing approximation formulas are difficult to accurately estimate the anisotropic parameters of reservoirs,even more so for unconventional reservoirs wit...In VTI media,the conventional inversion methods based on the existing approximation formulas are difficult to accurately estimate the anisotropic parameters of reservoirs,even more so for unconventional reservoirs with strong seismic anisotropy.Theoretically,the above problems can be solved by utilizing the exact reflection coefficients equations.However,their complicated expression increases the difficulty in calculating the Jacobian matrix when applying them to the Bayesian deterministic inversion.Therefore,the new reduced approximation equations starting from the exact equations are derived here by linearizing the slowness expressions.The relatively simple form and satisfactory calculation accuracy make the reduced equations easy to apply for inversion while ensuring the accuracy of the inversion results.In addition,the blockiness constraint,which follows the differentiable Laplace distribution,is added to the prior model to improve contrasts between layers.Then,the concept of GLI and an iterative reweighted least-squares algorithm is combined to solve the objective function.Lastly,we obtain the iterative solution expression of the elastic parameters and anisotropy parameters and achieve nonlinear AVA inversion based on the reduced equations.The test results of synthetic data and field data show that the proposed method can accurately obtain the VTI parameters from prestack AVA seismic data.展开更多
AIM To investigate the protective mechanism of mitofusin-2 (Mfn2) in rat remote ischemic perconditioning (RIC) models and revalidate it in alpha mouse liver-12 (AML-12) hypoxia cell lines. METHODS Sprague-Dawley rats ...AIM To investigate the protective mechanism of mitofusin-2 (Mfn2) in rat remote ischemic perconditioning (RIC) models and revalidate it in alpha mouse liver-12 (AML-12) hypoxia cell lines. METHODS Sprague-Dawley rats were divided into three groups (n = 6 each): sham, orthotopic liver transplantation and RIC. After operation, blood samples were collected to test alanine aminotransferase and aspartate aminotransferase. The liver lobes were harvested for histopathological examination, western blotting (WB) and quantitative real-time (qRT)-PCR. AML-12 cell lines were then subjected to normal culture, anoxic incubator tank culture (hypoxia) and anoxic incubator tank culture with Mfn2 knockdown (hypoxia + Si), and data of qRT-PCR, WB, mitochondrial membrane potential (Delta psi m), apoptosis, endoplasmic reticulum Ca2+ concentrations and mitochondrial Ca2+ concentrations were collected. RESULTS Both sham and normal culture groups showed no injury during the experiment. The RIC group showed amelioration of liver function compared with the orthotopic liver transplantation group (P < 0.05). qRTPCR and WB confirmed that Mfn2-mitochondrial Ca2+ uptake 1/2 (MICUs) axis was changed (P < 0.005). In AML-12 cell lines, compared with the hypoxia group, the hypoxia + Si group attenuated the collapse of..m and apoptosis (P < 0.005). The endoplasmic reticulum Ca2+ decrease and mitochondrial Ca2+ overloading observed in the hypoxia group were also attenuated in the hypoxia + Si group (P < 0.005). Finally, qRT-PCR and WB confirmed the Mfn2-MICUs axis change in all the groups (P < 0.005). CONCLUSION Mfn2 participates in liver injury in rat RIC models and AML-12 hypoxia cell lines by regulating the MICUs pathway.展开更多
Background: With the increased use of extended-criteria donors, static cold storage has failed to provide optimal preservation of liver grafts, resulting in early allograft dysfunction and long-term complications Mach...Background: With the increased use of extended-criteria donors, static cold storage has failed to provide optimal preservation of liver grafts, resulting in early allograft dysfunction and long-term complications Machine perfusion(MP) is a beneficial alternative preservation strategy for donor livers, particularly fo those considered to be of suboptimal quality, and could expand the limited donor pool. Data sources: A comprehensive search in Pub Med, EMBASE, Ovid databases and Clinical Trials.gov website was conducted using the medical subject heading terms "machine perfusion", "machine preservation""liver transplantation", combined with free text terms such as "hypothermic", "normothermic" and "sub normothermic". The deadline for the search was September 30, 2017. Results: MP can be classified as hypothermic, subnormothermic, and normothermic with the tempera ture maintained at 0–12 °C, 25–34 °C and 35–38 °C, respectively. Twelve clinical trials of MP have been reported in recent years. MP effectively decreased AST/ALT level and the incidence of early allograft dys function. However, the graft and patient survival rate after MP were similar to static cold storage. The detailed clinical characteristics such as liver function, graft survival, patient survival and early allograf dysfunction were reviewed.Conclusions: Clinical trial results showed that MP improves delayed graft function, primary non-function and biliary strictures. However, MP still requires validation in large clinical trials and the key parameters during MP still require optimization.展开更多
AIM：To investigate the relationship between high myopia [with or without complete congenital stationary night blindness（CSNB1）] and TRPM1 and NYX.METHODS： Two unrelated families with early-onset high myopia（eo HM...AIM：To investigate the relationship between high myopia [with or without complete congenital stationary night blindness（CSNB1）] and TRPM1 and NYX.METHODS： Two unrelated families with early-onset high myopia（eo HM） and 96 normal controls were recruited.Sanger sequencing or clone sequencing were used for mutation screening.Further analyses of the available family members and the 96 normal controls were subsequently conducted to obtain additional evidence of the pathogenicity of these variants.The initial diagnosis of the probands was eo HM.We performed a further comprehensive examination of the available family members after mutations were detected in TRPM1 or NYX. RESULTS： Two novel compound heterozygous mutations in TRPM1 were detected in the recruited families.The proband in family A with eo HM carried a c.2594 C 〉T missense mutation in exon 19 and a c.669 ＋3_669 ＋6del AAGT splicing mutation,which was co-segregated with CSNB1 in this family.A patient in family B with a compound heterozygous missense mutation（c.3262 G〉A and c.3250 T〉C） was detected.No mutations were found in NYX.These two identified compound heterozygous mutations were not found in the 96 normal controls.After further examination of the family members,the patients in family A could be diagnosed as eo HM with CSNB1.However due to the limited clinic data,the patient in family B cloud not clearly diagnosed as CSNB1.CONCLUSION： This study has expanded the mutation spectrum of TRPM1 for CSNB1 and additional studiesare needed to elucidate the association between isolated high myopia and TRPM1 and NYX.展开更多
Four new 9,19-cycloartane triterpenoids,cimilactone E(1),cimilactone F(2),20-O-(E)-butenoyl-23-epi-26-deoxyactein(3),and 20,12b-O-diacetylcimiracemonol-3-O-b-D-xylopyranoside(4),together with four known constituents(5...Four new 9,19-cycloartane triterpenoids,cimilactone E(1),cimilactone F(2),20-O-(E)-butenoyl-23-epi-26-deoxyactein(3),and 20,12b-O-diacetylcimiracemonol-3-O-b-D-xylopyranoside(4),together with four known constituents(5–8)were isolated from the roots of Cimicifuga foetida.The new structures were elucidated by extensive spectroscopic analysis.In addition,compounds 7 and 8 showed significant Wnt signaling pathway inhibitory activity,with IC50 values of 3.33 and 13.34 lM,respectively,using the luciferase reporter gene assay.展开更多
To the Editor:Hepatic ischemia-reperfusion(I/R)injury is the component of liver injury related to liver transplantation and liver surgery[1-3].The hepatic sinus microcirculation injury is a central part of hep-atic I/...To the Editor:Hepatic ischemia-reperfusion(I/R)injury is the component of liver injury related to liver transplantation and liver surgery[1-3].The hepatic sinus microcirculation injury is a central part of hep-atic I/R injury,which eventually leads to hepatocyte injury.This process is closely associated with the participation of liver non-parenchymal cells such as Kuffer cells,hepatic stellate cells,liver sinusoidal endothelial cells(LSECs),and cytokines.展开更多
Three new physalin steroids,physalin Ⅲ(1),physalin IV(2),3-O-methylphysalin X(3),together with five known physalins(4-8)were isolated from the 80%EtOH extract of calyces of Physalis alkekengi var.franchetii.The struc...Three new physalin steroids,physalin Ⅲ(1),physalin IV(2),3-O-methylphysalin X(3),together with five known physalins(4-8)were isolated from the 80%EtOH extract of calyces of Physalis alkekengi var.franchetii.The structures of the new compounds were revealed through 1D and 2D NMR and mass spectroscopic studies.展开更多
Genetic diversity and relationship of 183 tea germplasms were assessed by inter-simple sequence repeat(ISSR) marker.The study optimized the ISSR-PCR system for tea plants.Results show that genetic similarity among 183...Genetic diversity and relationship of 183 tea germplasms were assessed by inter-simple sequence repeat(ISSR) marker.The study optimized the ISSR-PCR system for tea plants.Results show that genetic similarity among 183 tea ascensions was between 0.52-0.93,indicating a wide gene pool and a broad genetic variation of Wuling tea resources.The highest similarity of all materials between No1 and No2 was 0.93.Dendrogram of the183 tea germplasms were protracted by UPGMA clustering analysis also revealed the genetic diversity and relationship of the tea germplasms of Wuling mountainous area.Besides,fingerprint of the 183 tea germplasms were constructed.展开更多
To the Editor:Peliosis hepatis(PH)is an uncommon benign vascular disorder characterized by widespread blood-filled cysts in the liver.This dis-ease was first described by Wagner in 1861,and because the liver lesions w...To the Editor:Peliosis hepatis(PH)is an uncommon benign vascular disorder characterized by widespread blood-filled cysts in the liver.This dis-ease was first described by Wagner in 1861,and because the liver lesions were generally red or blue-purple,it was first named PH by Schoenlack in 1916[1].The etiology of PH is not completely clear now,which is mainly related to drug factors,immune fac-tors,and infections.The diagnosis of PH is difficult because of the lack of specific clinical manifestations and imaging features.展开更多
基金Scientific Research Foundation for High-level Talents of Anhui University of Science and Technology(Grant No.2021yjrc38)Anhui Provincial Natural Science Foundation(Grant No.2208085QA27)+1 种基金National Natural Science Foundation of China(Grant Nos.11972046,12002266)the authors would like to thank these foundations for financial support.
文摘2,4-dinitroanisole(DNAN)is a good replacement for 2,4,6-trinitrotoluene(TNT)in melt-cast explosives due to its superior insensitivity.With the increasing use of DNAN-based melt-cast explosives,the prediction of reaction violence and hazard assessment of the explosives subjected to shock is of great significance.This study investigated the shock initiation characteristics for a DNAN-based melt-cast explosive,DHFA,using the one-dimensional Lagrangian apparatus.The embedded manganin gauges in the apparatus record the pressure histories at four Lagrangian positions and show that shock-todetonation transition in DHFA needs a high input shock pressure.The experimental data are analyzed to calibrate the Ignition and Growth model.The calibration is performed using an objective function based on both pressure history and the arrival time of shock.Good agreement between experimental and calculated pressure histories indicates the high accuracy of the calibrated parameters with the optimization method.
基金Natural Science Foundation of Sichuan Province(No.2022NSFSC1370).
文摘Dear Editor,Tuberculosis(TB)is an infectious disease which has been considered as a global public health emergency problem during the past 25y.The World Health Organization has estimated that approximately one-third of the world’s population are infected by TB.However,only 10%of patients with TB is symptomatic.TB can affect multiple organs throughout the body.Eighty percent of those infected are pulmonary TB.Ocular TB can occur with or without lung or other systemic TB.It is a rare extrapulmonary form of the disease and occupied about 3.5%–5%of all of the TB.It should not be disregarded as its potential influence on vision loss.The intraocular and/or extraocular structures could be impacted by ocular TB[1].Uvea was the most common affected structure[2].
基金approved by Jiangxi Provincial People’s Hospital and First Affiliated Hospital,Zhejiang University School of Medicine(2022068 and 2022370).Written informed consent was obtained from all participants.
文摘Background:Wernicke encephalopathy(WE)is an acute neurological disease resulting from vitamin B1 deficiency,and there are only very few case reports of WE after liver transplantation.The present study aimed to investigate the clinical characteristics,etiology,magnetic resonance imaging(MRI)features,treatment and prognosis of patients with WE after liver transplantation.Methods:Twenty-three patients with WE after liver transplantation from the First Affiliated Hospital,Zhejiang University School of Medicine and Jiangxi Provincial People’s Hospital between January 2011 and December 2021 were retrospectively analyzed.Results:Among the 23 patients diagnosed with WE after liver transplantation,6(26%)had a classic triad of impaired consciousness,oculomotor palsy and ataxia,and 17(74%)had two features.The misdiagno-sis rate was 65%.After treatment with high-dose vitamin B1,19(83%)patients showed improvement,whereas 4(17%)showed no improvement,including 3 with residual short-term memory impairments and 1 with residual spatial and temporal disorientation and ataxia.Conclusions:The misdiagnosis rate is high in the early stage of WE,and the prognosis is closely asso-ciated with whether WE is diagnosed early and treated timely.High-dose glucose or glucocorticoids can trigger WE and cannot be administered before vitamin B1 treatment.Vitamin B1 is suggested to be used as a prophylactic treatment for patients with WE after liver transplantation.
文摘Uncertainty is an important characteristic of scheduling model for scale farm machinery operation organizing. Practice shows that scheduling model without considering uncertainties is nearly useless. Uncertain influence factors arisen from natural environment, society and economy, market and supply, and customer and behavior, exist widely, emerge frequently, and affect production deeply. Uncertainties interfere with the allocation of productive factors on temporal and spatial dimensions for farm machinery operation scheduling and management. Questionnaire for farm machinery organizations was designed and finished in 2014. Both occurrence frequency and influence degree for each factor were quantified. Four influence factors including operation location change, weather mutation, parts supply delay, and operation skill defects appear in both list of high occurrence and deep influence. Then results of questionnaire and results of specific investigation were used to study temporal and spatial scheduling model and system for farm machinery management. Three case studies are introduced. The first case is about the uncertainty and countermeasure of forage harvesters scheduling and monitoring for a professional forage plantation company. The second case is about the uncertainty and counter measure of cotton-picker scheduling and monitoring for a professional cotton picking company. And the third case is about the uncertainty and countermeasure of social service management for a professional cooperative. The cases show that the research has strong pertinence to deal with uncertainties and can improve management efficiency of farm machinery operation.
基金supported by the National Natural Science Foundation of China(22278059,22174009,and 22078047)Fundamental Research Funds for the Central Universities(DUT22LAB601 and DUT22LAB608)。
文摘The construction of a stable-membrane tracker has significant implications for the visualization of the membrane in live cells.However,most current plasma trackers are not suitable for tracking plasma membranes for a long time due to their limited retention time.Herein,Mem580-F-Sulfo is designed to target and anchor cell membranes and therefore track cell membranes for a longer time.This tracker is composed of a lipophilic boron-dipyrromethene(BODIPY)derivative and a hydrophilic zwitterion to form an amphiphilic structure,which enables its targeting ability toward cell membranes.Moreover,a reactive ester group is included to bind with proteins through covalent bonds in cell membranes nonspecifically,which extends retention time in cell membranes.Mem580-F-Sulfo shows intense brightness(94600),with a high molar absorption coefficient of up to about 100000 L·mol^(-1)·cm^(-1)and a fluorescence quantum yield of up to 0.97.It shows fast cell membrane targeting ability and long retention up to 90 min.In brief,this work has not only developed a tracker with good cell membrane targetability but also provided a new strategy for improving the targeting stability of cell membranes.
文摘It is a major challenge for the airframe-inlet design of modern combat aircrafts,as the flow and electromagnetic wave propagation in the inlet of stealth aircraft are very complex.In this study,an aerodynamic/stealth optimization design method for an S-duct inlet is proposed.The upwind scheme is introduced to the aerodynamic adjoint equation to resolve the shock wave and flow separation.The multilevel fast multipole algorithm(MLFMA)is utilized for the stealth adjoint equation.A dorsal S-duct inlet of flying wing layout is optimized to improve the aerodynamic and stealth characteristics.Both the aerodynamic and stealth characteristics of the inlet are effectively improved.Finally,the optimization results are analyzed,and it shows that the main contradiction between aerodynamic characteristics and stealth characteristics is the centerline and crosssectional area.The S-duct is smoothed,and the cross-sectional area is increased to improve the aerodynamic characteristics,while it is completely opposite for the stealth design.The radar cross section(RCS)is reduced by phase cancelation for low frequency conditions.The method is suitable for the aerodynamic/stealth design of the aircraft airframe-inlet system.
文摘BACKGROUND Cholesterol stones affect a certain subpopulation of children.Concerns have been raised on the impact of gallbladder surgery on the growth of children and adolescents.AIM To study the population characteristics,clinical features,treatment,and prognosis of gallstones in children.METHODS The clinical data of 44 children with gallstones admitted to The First Affiliated Hospital of Naval Medical University from August 2009 to August 2021 were collected,the children were followed up by telephone to monitor their prognoses.The follow-up ended in August 2023.The shortest follow-up time was 2 years and 6 months,whereas the longest was 13 years and 11 months.The population characteristics,general clinical characteristics,and treatments were retrospectively analyzed.The children were divided according to whether they underwent surgical gallbladder removal into an operation group(n=28)and a non-operation group(n=16),The effects of surgical gallbladder resection on the growth and development of children were analyzed.RESULTS The male–female ratio in the population was 6:5 and 84.09%of the children had onset in adolescence.Furthermore,29.55%of the children were overweight or obese.The study identified 26 cases with metabolic abnormalities,9 with hemolytic anemia,and 4 with choledochal cyst.Of the population,68.18%had recurrent symptomatic cholecystolithiasis.Surgical treatment accounted for 63.64%,with laparoscopic cholecystectomy accounting for 71.43%of surgical treatment.No significant differences were observed in symptoms and complications between the surgery and non-surgery groups.Furthermore,no significant differences were found between the two groups in the attainment of genetic height target and the rightward shift of height curve during follow-up.CONCLUSION The sex characteristics of gallstones in children were not observed.Most gallstones occurred in adolescents and rarely in young children.A considerable proportion of children have inborn causes,which are often concurrent with metabolic abnormalities and hemolytic anemia.Most children had recurrent symptomatic gallstones.Surgical treatment,especially laparoscopic cholecystectomy,is still the main treatment for gallstones in children.Surgical treatment did not affect the growth and development of children who underwent gallstone removal.
文摘目的探讨全程多学科营养模式在老年患者髋部手术中的应用效果。方法选取2021年10月至2023年12月于温州市中心医院行髋部手术的老年患者90例,根据随机数字表法将其分为对照组和研究组,每组各45例。对照组患者应用饮食管理模式,研究组患者应用全程多学科营养模式。比较两组患者的恢复情况、营养状况、生活质量、髋关节功能和满意度。结果研究组患者的住院时间、骨折愈合时间均显著短于对照组(P<0.05)。干预后,两组患者的前白蛋白(prealbumin,PA)、白蛋白(albumin,Alb)、血红蛋白(hemoglobin,Hb)、健康调查量表36(36-item short form health survey,SF-36)各维度评分、Harris髋关节评分均显著高于本组干预前(P<0.05),且研究组患者的PA、Alb、Hb、SF-36各维度评分、Harris髋关节评分均显著高于对照组(P<0.05)。研究组患者的满意度显著高于对照组(χ^(2)=5.075,P=0.024)。结论老年患者髋部手术中应用全程多学科营养模式可改善其营养状态,提升生活质量,增强髋关节功能,有利于术后恢复。
文摘In VTI media,the conventional inversion methods based on the existing approximation formulas are difficult to accurately estimate the anisotropic parameters of reservoirs,even more so for unconventional reservoirs with strong seismic anisotropy.Theoretically,the above problems can be solved by utilizing the exact reflection coefficients equations.However,their complicated expression increases the difficulty in calculating the Jacobian matrix when applying them to the Bayesian deterministic inversion.Therefore,the new reduced approximation equations starting from the exact equations are derived here by linearizing the slowness expressions.The relatively simple form and satisfactory calculation accuracy make the reduced equations easy to apply for inversion while ensuring the accuracy of the inversion results.In addition,the blockiness constraint,which follows the differentiable Laplace distribution,is added to the prior model to improve contrasts between layers.Then,the concept of GLI and an iterative reweighted least-squares algorithm is combined to solve the objective function.Lastly,we obtain the iterative solution expression of the elastic parameters and anisotropy parameters and achieve nonlinear AVA inversion based on the reduced equations.The test results of synthetic data and field data show that the proposed method can accurately obtain the VTI parameters from prestack AVA seismic data.
基金Supported by Science and Technology Innovation Talents Support Plan,Department of Education,Henan Province,China,No.17HASTIT044China Postdoctoral Science Foundation,No.2017M610374
文摘AIM To investigate the protective mechanism of mitofusin-2 (Mfn2) in rat remote ischemic perconditioning (RIC) models and revalidate it in alpha mouse liver-12 (AML-12) hypoxia cell lines. METHODS Sprague-Dawley rats were divided into three groups (n = 6 each): sham, orthotopic liver transplantation and RIC. After operation, blood samples were collected to test alanine aminotransferase and aspartate aminotransferase. The liver lobes were harvested for histopathological examination, western blotting (WB) and quantitative real-time (qRT)-PCR. AML-12 cell lines were then subjected to normal culture, anoxic incubator tank culture (hypoxia) and anoxic incubator tank culture with Mfn2 knockdown (hypoxia + Si), and data of qRT-PCR, WB, mitochondrial membrane potential (Delta psi m), apoptosis, endoplasmic reticulum Ca2+ concentrations and mitochondrial Ca2+ concentrations were collected. RESULTS Both sham and normal culture groups showed no injury during the experiment. The RIC group showed amelioration of liver function compared with the orthotopic liver transplantation group (P < 0.05). qRTPCR and WB confirmed that Mfn2-mitochondrial Ca2+ uptake 1/2 (MICUs) axis was changed (P < 0.005). In AML-12 cell lines, compared with the hypoxia group, the hypoxia + Si group attenuated the collapse of..m and apoptosis (P < 0.005). The endoplasmic reticulum Ca2+ decrease and mitochondrial Ca2+ overloading observed in the hypoxia group were also attenuated in the hypoxia + Si group (P < 0.005). Finally, qRT-PCR and WB confirmed the Mfn2-MICUs axis change in all the groups (P < 0.005). CONCLUSION Mfn2 participates in liver injury in rat RIC models and AML-12 hypoxia cell lines by regulating the MICUs pathway.
基金supported by grants from the National Natural Science Foundation of China(81470891)China Postdoctoral Science Foundation(2017M610374)+1 种基金Science and Technology Bureau of Zhejiang Province,China(2016C33145)Innovative Research Groups of the National Natural Science Foundation of China(81421062)
文摘Background: With the increased use of extended-criteria donors, static cold storage has failed to provide optimal preservation of liver grafts, resulting in early allograft dysfunction and long-term complications Machine perfusion(MP) is a beneficial alternative preservation strategy for donor livers, particularly fo those considered to be of suboptimal quality, and could expand the limited donor pool. Data sources: A comprehensive search in Pub Med, EMBASE, Ovid databases and Clinical Trials.gov website was conducted using the medical subject heading terms "machine perfusion", "machine preservation""liver transplantation", combined with free text terms such as "hypothermic", "normothermic" and "sub normothermic". The deadline for the search was September 30, 2017. Results: MP can be classified as hypothermic, subnormothermic, and normothermic with the tempera ture maintained at 0–12 °C, 25–34 °C and 35–38 °C, respectively. Twelve clinical trials of MP have been reported in recent years. MP effectively decreased AST/ALT level and the incidence of early allograft dys function. However, the graft and patient survival rate after MP were similar to static cold storage. The detailed clinical characteristics such as liver function, graft survival, patient survival and early allograf dysfunction were reviewed.Conclusions: Clinical trial results showed that MP improves delayed graft function, primary non-function and biliary strictures. However, MP still requires validation in large clinical trials and the key parameters during MP still require optimization.
基金Supported by the National Nature Science Foundation of China(No.81362138)
文摘AIM：To investigate the relationship between high myopia [with or without complete congenital stationary night blindness（CSNB1）] and TRPM1 and NYX.METHODS： Two unrelated families with early-onset high myopia（eo HM） and 96 normal controls were recruited.Sanger sequencing or clone sequencing were used for mutation screening.Further analyses of the available family members and the 96 normal controls were subsequently conducted to obtain additional evidence of the pathogenicity of these variants.The initial diagnosis of the probands was eo HM.We performed a further comprehensive examination of the available family members after mutations were detected in TRPM1 or NYX. RESULTS： Two novel compound heterozygous mutations in TRPM1 were detected in the recruited families.The proband in family A with eo HM carried a c.2594 C 〉T missense mutation in exon 19 and a c.669 ＋3_669 ＋6del AAGT splicing mutation,which was co-segregated with CSNB1 in this family.A patient in family B with a compound heterozygous missense mutation（c.3262 G〉A and c.3250 T〉C） was detected.No mutations were found in NYX.These two identified compound heterozygous mutations were not found in the 96 normal controls.After further examination of the family members,the patients in family A could be diagnosed as eo HM with CSNB1.However due to the limited clinic data,the patient in family B cloud not clearly diagnosed as CSNB1.CONCLUSION： This study has expanded the mutation spectrum of TRPM1 for CSNB1 and additional studiesare needed to elucidate the association between isolated high myopia and TRPM1 and NYX.
基金Program for National Natural Science Foundation of China(Nos.U1132604 and 81302670)The Major Deployment Program of the CAS(No.KSZDEW-Z-004-01)Foundation of State Key Laboratory of Phytochemistry and Plant Resources in West China(P2008-ZZ05).
文摘Four new 9,19-cycloartane triterpenoids,cimilactone E(1),cimilactone F(2),20-O-(E)-butenoyl-23-epi-26-deoxyactein(3),and 20,12b-O-diacetylcimiracemonol-3-O-b-D-xylopyranoside(4),together with four known constituents(5–8)were isolated from the roots of Cimicifuga foetida.The new structures were elucidated by extensive spectroscopic analysis.In addition,compounds 7 and 8 showed significant Wnt signaling pathway inhibitory activity,with IC50 values of 3.33 and 13.34 lM,respectively,using the luciferase reporter gene assay.
基金supported by grants from the Scientific Research Foundation of Traditional Chinese Medicine of Zhejiang Province(2015ZA085)Scientific Research Project of Zhejiang Provincial Health Commission(2021448467)the Basic Public Welfare Re-search Project of Zhejiang Province(LGF20H030011).
文摘To the Editor:Hepatic ischemia-reperfusion(I/R)injury is the component of liver injury related to liver transplantation and liver surgery[1-3].The hepatic sinus microcirculation injury is a central part of hep-atic I/R injury,which eventually leads to hepatocyte injury.This process is closely associated with the participation of liver non-parenchymal cells such as Kuffer cells,hepatic stellate cells,liver sinusoidal endothelial cells(LSECs),and cytokines.
基金This project was financially supported by the National Major Basic Research Program(No.SB2007FY400)the National Ministry of Science and Technology of China,the National Knowledge Innovation Program of Chinese Academy of Sciences(No.KSCX2-YW-G-038)the Foundation of State Key Laboratory of Phytochemistry and Plant Resources in West China(P2010-ZZ14).
文摘Three new physalin steroids,physalin Ⅲ(1),physalin IV(2),3-O-methylphysalin X(3),together with five known physalins(4-8)were isolated from the 80%EtOH extract of calyces of Physalis alkekengi var.franchetii.The structures of the new compounds were revealed through 1D and 2D NMR and mass spectroscopic studies.
基金supported by the earmarked fund for Modern Agro-industry Technology Research System of China (CARS-23)the Priority Academic Program Development of Jiangsu Higher Education Institutions+2 种基金the Science and Technology Program of Suzhou (SZGD201067,WZC65,WNZ1002)The PhD Gathering Program of Jiangsuthe Science and Technology Program of Jiangsu Province (BE2011319,BE2012440,BE2013426)
文摘Genetic diversity and relationship of 183 tea germplasms were assessed by inter-simple sequence repeat(ISSR) marker.The study optimized the ISSR-PCR system for tea plants.Results show that genetic similarity among 183 tea ascensions was between 0.52-0.93,indicating a wide gene pool and a broad genetic variation of Wuling tea resources.The highest similarity of all materials between No1 and No2 was 0.93.Dendrogram of the183 tea germplasms were protracted by UPGMA clustering analysis also revealed the genetic diversity and relationship of the tea germplasms of Wuling mountainous area.Besides,fingerprint of the 183 tea germplasms were constructed.
文摘To the Editor:Peliosis hepatis(PH)is an uncommon benign vascular disorder characterized by widespread blood-filled cysts in the liver.This dis-ease was first described by Wagner in 1861,and because the liver lesions were generally red or blue-purple,it was first named PH by Schoenlack in 1916[1].The etiology of PH is not completely clear now,which is mainly related to drug factors,immune fac-tors,and infections.The diagnosis of PH is difficult because of the lack of specific clinical manifestations and imaging features.
