Glucosinolates(GLS) contribute to the unique flavour, nutrition, and plant defence of the Cruciferous vegetables. Understanding the GLS changes through postharvest processing is essential for defined preservation. In ...Glucosinolates(GLS) contribute to the unique flavour, nutrition, and plant defence of the Cruciferous vegetables. Understanding the GLS changes through postharvest processing is essential for defined preservation. In this study, four different fresh-cut types, whole flower(W),floret(F), quarterly cut floret(QF) and shredded floret(FS) of broccoli, were stored for 0, 1, 2 and 3 day(s) to explore GLS responses to postharvest treatments. As a result, seven GLS were identified, mainly including glucoraphanin(RAA), neoglucobrassicin(NEO), and glucobrassicin(GBC)and accounting for 52.69%, 20.12% and 14.99% of the total GLS(21.92 ± 0.48) μmol · g ^(-1 )DW, respectively. FS had the sharpest decrease in GLS after three days of storage(6.55 ± 0.37) μmol · g-1DW, while QF had the least(10.16 ± 0.33) μmol · g ^(-1 )DW. All GLS components decreased over storage, except for 4-methoxyglucobrassicin(4 ME) in FS and QF, suggesting its key role in serious wound defence. The results suggested certain postharvest approaches influenced the flavour and nutrition of broccoli.展开更多
The research aimed to develop and validate an ion chromatography method for the simultaneous analysis of Cr(Ⅲ) and Cr(Ⅵ)from pumpkin. A new analytical method based on ion chromatography techniques was developed by t...The research aimed to develop and validate an ion chromatography method for the simultaneous analysis of Cr(Ⅲ) and Cr(Ⅵ)from pumpkin. A new analytical method based on ion chromatography techniques was developed by the Cr(Ⅲ) pre-column derivatization and Cr(Ⅵ) post-column derivatization. The ion chromatography condition was optimized and the detection sensitivity was improved. Cr(Ⅲ) and Cr(Ⅵ) were determined by 365 and 530 nm,respectively. The temperature of water bath,the heating time for pre-column derivatization,and the flow rate of post-column derivative liquid were screened on the basis of single factor experiment,the effects of various factors were determined by the method of L16( 43) orthogonal experiment design. Considering the results of orthogonal experiments and the variation tendency of peak area under different factors,the optimum derivatization conditions were chose as follows: the flow rate of post-column derivative liquid is0. 5 m L/min,the temperature of water bath for pre-column derivatization is 100 ℃ and the heating time is 5 min. The conditions were optimized by means of orthogonal experiments under the p H of leacheate ranged from 6. 5 to 6. 8 and the I-concentration of 5 mmol/L. Under optimized derivatization conditions,the detection limits for Cr(Ⅲ) and Cr(Ⅵ) were 0. 17 and 0. 019 mg,respectively. And the average recoveries of Cr(Ⅲ) in pumpkin were in the range of 82%-85%. Results indicated that pumpkin does not contain Cr(Ⅵ) according to this ion chromatography method of simultaneously determination for Cr(Ⅲ) and Cr(Ⅵ).展开更多
Glucosinolate composition and content were evaluated in flowers and leaves of 12 different broccoli varieties. The results indicated that there were 9 glucosinolates in broccoli,namely Glucoiberin(IBE); Progoitrin(PRO...Glucosinolate composition and content were evaluated in flowers and leaves of 12 different broccoli varieties. The results indicated that there were 9 glucosinolates in broccoli,namely Glucoiberin(IBE); Progoitrin(PRO);Sinigrin(SIN);Glucoraphanin(RAA);Gluconapin(NAP);4-Hydroxyglucobrassicin(4OH);Glucobrassicin(GBC);4-Methoxyglucobrassicin(4ME);Neoglucobrassicin(NEO).Total glucosinolate content in flowers was 1-5 times higher than in leaves. The predominant glucosinolate in broccoli was glucoraphanin.展开更多
X chromosomal short tandem repeats(X-STRs)have the characteristics of both autosomal and uniparental genetic markers and have been shown to be particularly useful in forensic casework.However,relevant research or repo...X chromosomal short tandem repeats(X-STRs)have the characteristics of both autosomal and uniparental genetic markers and have been shown to be particularly useful in forensic casework.However,relevant research or reports have not focused on X-STRs in the Hani population.To investigate the genetic variation and forensic efficiency of 16 X-STR loci in the Hani ethnic minority,we calculated the allele frequencies and forensic parameters of 451(116 males and 335 females)unrelated healthy Hani individuals from Yunnan Province,Southwest China.All these loci are highly polymorphic in Hani individuals in Yunnan Province except DXS6800.The combined power of discrimination in males(PDM)and power of discrimination in females(PDF)were found to be 0.999999998433993 and 0.999999999999998,respectively.Furthermore,a population genetic structure investigation between the Yunnan Hani population and another 18 populations was performed using a principal component analysis,multidimensional scaling plot and neighbouring-joining phylogenetic tree and the findings illustrated that neighbouring populations and different nationalities in the same area appeared to have a closer evolutionary relationship.This study provides the first batch of X chromosome genetic polymorphism data of the Hani population in Yunnan Province,Southwest China and enriches the reference database of the Chinese minority population.展开更多
基金supported by the Collaborative Innovation Center of the Beijing Academy of Agriculture and Forestry Sciences(Grant No.KJCX201915)the Youth Scientific Research Funds of Beijing Academy of Agriculture and Forestry Sciences(Grant No.QNJJ201914)the Innovation and Capacity-building Project of Beijing Academy of Agriculture and Forestry Sciences(Grant No.KJCX20200213).
文摘Glucosinolates(GLS) contribute to the unique flavour, nutrition, and plant defence of the Cruciferous vegetables. Understanding the GLS changes through postharvest processing is essential for defined preservation. In this study, four different fresh-cut types, whole flower(W),floret(F), quarterly cut floret(QF) and shredded floret(FS) of broccoli, were stored for 0, 1, 2 and 3 day(s) to explore GLS responses to postharvest treatments. As a result, seven GLS were identified, mainly including glucoraphanin(RAA), neoglucobrassicin(NEO), and glucobrassicin(GBC)and accounting for 52.69%, 20.12% and 14.99% of the total GLS(21.92 ± 0.48) μmol · g ^(-1 )DW, respectively. FS had the sharpest decrease in GLS after three days of storage(6.55 ± 0.37) μmol · g-1DW, while QF had the least(10.16 ± 0.33) μmol · g ^(-1 )DW. All GLS components decreased over storage, except for 4-methoxyglucobrassicin(4 ME) in FS and QF, suggesting its key role in serious wound defence. The results suggested certain postharvest approaches influenced the flavour and nutrition of broccoli.
基金Supported by Science and Technology Innovation Ability Construction Special Item of Beijing Academy of Agriculture and Forestry Sciences(KJCX20140302,KJCX20140111)
文摘The research aimed to develop and validate an ion chromatography method for the simultaneous analysis of Cr(Ⅲ) and Cr(Ⅵ)from pumpkin. A new analytical method based on ion chromatography techniques was developed by the Cr(Ⅲ) pre-column derivatization and Cr(Ⅵ) post-column derivatization. The ion chromatography condition was optimized and the detection sensitivity was improved. Cr(Ⅲ) and Cr(Ⅵ) were determined by 365 and 530 nm,respectively. The temperature of water bath,the heating time for pre-column derivatization,and the flow rate of post-column derivative liquid were screened on the basis of single factor experiment,the effects of various factors were determined by the method of L16( 43) orthogonal experiment design. Considering the results of orthogonal experiments and the variation tendency of peak area under different factors,the optimum derivatization conditions were chose as follows: the flow rate of post-column derivative liquid is0. 5 m L/min,the temperature of water bath for pre-column derivatization is 100 ℃ and the heating time is 5 min. The conditions were optimized by means of orthogonal experiments under the p H of leacheate ranged from 6. 5 to 6. 8 and the I-concentration of 5 mmol/L. Under optimized derivatization conditions,the detection limits for Cr(Ⅲ) and Cr(Ⅵ) were 0. 17 and 0. 019 mg,respectively. And the average recoveries of Cr(Ⅲ) in pumpkin were in the range of 82%-85%. Results indicated that pumpkin does not contain Cr(Ⅵ) according to this ion chromatography method of simultaneously determination for Cr(Ⅲ) and Cr(Ⅵ).
基金Supported by Special Non-profit Sector Project of the Ministry of Agriculture(20130309)Special Science and Technology Innovation Capacity Building Project of Beijing Academy of Agriculture and Forestry Sciences(KJCX20140111)
文摘Glucosinolate composition and content were evaluated in flowers and leaves of 12 different broccoli varieties. The results indicated that there were 9 glucosinolates in broccoli,namely Glucoiberin(IBE); Progoitrin(PRO);Sinigrin(SIN);Glucoraphanin(RAA);Gluconapin(NAP);4-Hydroxyglucobrassicin(4OH);Glucobrassicin(GBC);4-Methoxyglucobrassicin(4ME);Neoglucobrassicin(NEO).Total glucosinolate content in flowers was 1-5 times higher than in leaves. The predominant glucosinolate in broccoli was glucoraphanin.
基金supported by the Strategic Priority Research Program of the Chinese Academy of Sciences(XDB29040201)the National Natural Science Foundation of China(NSFC)(81901680)。
基金This work was supported by the National Natural Science Foundation of China[grant numbers 81860258 and 31860300]Joint Special Funds for the Department of Science and Technology of Yunnan Province-Kunming Medical University[grant numbers 2017FE467(-022),2018FE001(-131)and 2019FE001(-011)]+3 种基金Yunnan Educational Science Foundation program[grant numbers 2018JS170 and 2018JS171]Yunnan Provincial Young Academic and Technical Leader Reserve Talent Project[grant number 2018HB104]Hundred-Talent Program of Kunming Medical University[grant number 60117190447]Science and Technology Innovation Group of Kunming Medical University[grant number CXTD201803].
文摘X chromosomal short tandem repeats(X-STRs)have the characteristics of both autosomal and uniparental genetic markers and have been shown to be particularly useful in forensic casework.However,relevant research or reports have not focused on X-STRs in the Hani population.To investigate the genetic variation and forensic efficiency of 16 X-STR loci in the Hani ethnic minority,we calculated the allele frequencies and forensic parameters of 451(116 males and 335 females)unrelated healthy Hani individuals from Yunnan Province,Southwest China.All these loci are highly polymorphic in Hani individuals in Yunnan Province except DXS6800.The combined power of discrimination in males(PDM)and power of discrimination in females(PDF)were found to be 0.999999998433993 and 0.999999999999998,respectively.Furthermore,a population genetic structure investigation between the Yunnan Hani population and another 18 populations was performed using a principal component analysis,multidimensional scaling plot and neighbouring-joining phylogenetic tree and the findings illustrated that neighbouring populations and different nationalities in the same area appeared to have a closer evolutionary relationship.This study provides the first batch of X chromosome genetic polymorphism data of the Hani population in Yunnan Province,Southwest China and enriches the reference database of the Chinese minority population.
