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MicroRNA-145-3p suppresses the malignant behaviors of T-cell acute lymphoblastic leukemia Jurkat cells via inhibiting the NF-kappaB signaling pathway 被引量:1
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作者 Xin YANG liqun lu +2 位作者 Li HUANG Jing HE Jie LV 《BIOCELL》 SCIE 2020年第1期101-110,共10页
T-cell acute lymphoblastic leukemia(T-ALL)is a hematological tumor caused by the malignant transformation of immature T-cell progenitor cells.Emerging studies have stated that microRNAs(miRNAs)may play key roles in T-... T-cell acute lymphoblastic leukemia(T-ALL)is a hematological tumor caused by the malignant transformation of immature T-cell progenitor cells.Emerging studies have stated that microRNAs(miRNAs)may play key roles in T-ALL progression.This study aimed to investigate the roles of miR-145-3p in T-ALL cell proliferation,invasion,and apoptosis with the involvement of the nuclear factor-kappaB(NF-κB)signaling pathway.T-ALL Jurkat cells were harvested,and the expression of miR-145-3p and NF-κB-p65 was measured.Gain-and loss-of-functions of miR-145-3p and NF-κB-p65 were performed to identify their roles in the biological behaviors of Jurkat cells,including proliferation,apoptosis,and invasion.Consequently,the current study demonstrated that miR-145-3p was down-regulated while NF-κB-p65 was up-regulated in Jurkat cells.miR-145-3p directly bound to the 3’untranslated region of NF-κB-p65.Over-expression of miR-145-3p inhibited Jurkat cell proliferation,invasion,and resistance to apoptosis,while over-expression of NF-κB-p65 presented opposite trends.Co-transfection of miR-145-3p and NF-κB-p65 promoted the malignant behaviors of Jurkat cells compared to miR-145-3p transfection alone,while it reduced these behaviors of Jurkat cells compared to NF-κB-p65 transfection alone.Taken together,this study provided evidence that miR-145-3p could suppress proliferation,invasion,and resistance to the death of T-ALL cells via inactivating the NF-κB signaling pathway. 展开更多
关键词 T-cell acute lymphoblastic leukemia Proliferation INVASION Resistance to apoptosis
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Identification of serum immunoglobulin M(IgM)of crucian carp(Carassius auratus gibelio)and immune response to cyprinid herpesvirus 2 infection
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作者 Shuran Wang Haotian Wu +5 位作者 Longlong Wang Pinhua Wang liqun lu Yousheng Jiang Ye Zhang Dan Xu 《Aquaculture and Fisheries》 CSCD 2024年第2期172-180,共9页
Crucian carp(Carassius auratus gibelio),an extensively cultivated freshwater fish,was one of the model species for the study of fish immunology.Polyclonal antibodies were advantageous molecular tools for studying tele... Crucian carp(Carassius auratus gibelio),an extensively cultivated freshwater fish,was one of the model species for the study of fish immunology.Polyclonal antibodies were advantageous molecular tools for studying teleost immune system.Specifically,polyclonal antibodies reacting with immunoglobulins(Ig)were used successfully in studies of the teleost fishes.In the present study,we produced polyclonal antibody against CH2 domains of crucian carp IgM,and measured the in vivo dynamics of IgM mRNA caused by CyHV-2 infection.The recombinant protein IgM with relative molecular weight about 53 KD was correctly expressed in prokaryotic cells.The specificity of the polyclonal antibody was evaluated by Western blotting and results revealed that the antibody not only specifically recognized crucian carp serum but also cross-reacted with grass carp serum.Quantitative RT-PCR analysis demonstrated the expression of IgM mRNA changed significantly after CyHV-2 infection.The expression of IgM in the kidney increased and reached a maximum at 6 h post-infection(hpi),while dropped to a low level at 5 days post-infection(dpi).In conclusion,the expression of IgM was significantly upregulated in the kidney of crucian carp infected with CyHV-2,indicating that IgM played a potential role in systemic immunity against viral infection.Polyclonal antibody against crucian carp IgM had certain clinical relevance,which might provide insight into the early stage of virus infection and prevention of the disease. 展开更多
关键词 Immunoglobulin M Crucian carp Polyclonal antibody Cyprinid herpesvirus 2 IMMUNOPROTECTION
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Detection of cotton waterlogging stress based on hyperspectral images and convolutional neural network 被引量:1
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作者 Jing Zhao Fangjiang Pan +4 位作者 Zhiming Li Yubin Lan liqun lu Dongjian Yang Yuting Wen 《International Journal of Agricultural and Biological Engineering》 SCIE EI CAS 2021年第2期167-174,共8页
Waterlogging in the early stage of cotton will reduce the number of bolls and do harm to yield.Early detection of waterlogging will help farmers to adjust cotton management and save the loss.To evaluate the applicatio... Waterlogging in the early stage of cotton will reduce the number of bolls and do harm to yield.Early detection of waterlogging will help farmers to adjust cotton management and save the loss.To evaluate the application of deep learning for the detection of early waterlogging,this study applied a convolutional neural network(CNN)to classify different durations of waterlogging stress(0,2,4,6,8,10 d)based on hyperspectral images(HSIs)of cotton leaves.An experiment was designed to simulate the situation of cotton under waterlogging stress and collect HSIs of visible and near-infrared(VNIR 450-950 nm)spectra with 126 bands 66 d after cotton sowing(66 DAS).It was found the spectral curve reflectance of waterlogging cotton was higher than that of non-waterlogging cotton.Especially near 550 nm and 750 nm,and the spectral curve increased with durations of waterlogging stress and there were‘blue shift’phenomena for the position of the red edge of the spectra.The first principal components of HSIs after band randomly discarding and principal component analysis(PCA)were used to build a dataset.GoogLeNet Inception-v3(GLNI-v3)and VGG-16 models were selected to detect cotton waterlogging stress with the dataset.The results showed that the average time for a round training for GLNI-v3 was 13.337 s,with a classification accuracy of 96.95%and a loss value of 0.09431.The average time for a round training for VGG-16 was 21.470 s,with a classification accuracy of 97.00%and a loss value of 0.17912.Though these two models had similar classification accuracy and loss value,GLNI-v3 achieved a high accuracy with fewer training iterations.The durations of waterlogging stress of cotton in a short-term can be detected by HSIs of cotton leaves and CNN models are suitable for the classification of HSIs,and this method can provide support for cotton yield estimation and loss assessment after waterlogging. 展开更多
关键词 COTTON WATERLOGGING hyperspectral image convolutional neural network
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New method for cotton fractional vegetation cover extraction based on UAV RGB images 被引量:1
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作者 Huanbo Yang Yubin Lan +3 位作者 liqun lu Daocai Gong Jianchi Miao Jing Zhao 《International Journal of Agricultural and Biological Engineering》 SCIE CAS 2022年第4期172-180,共9页
As the key principle of precision farming,the distribution of fractional vegetation cover is the basis of crop management within the field serves.To estimate crop FVC rapidly at the farm scale,high temporal-spatial re... As the key principle of precision farming,the distribution of fractional vegetation cover is the basis of crop management within the field serves.To estimate crop FVC rapidly at the farm scale,high temporal-spatial resolution imagery obtained by unmanned aerial vehicle(UAV)was adopted.To verify the application potential of consumer-grade UAV RGB imagery in estimated FVC,blue-green characteristic vegetation index(TBVI)and red-green vegetation index(TRVI)were proposed in this study according to the differences of the gray value among cotton vegetation,soil and shadow in the field.First,two new constructed indices and several published indices were used to extract visible light images and generate greyscale images for each of the visible light vegetation indices.Then,the thresholds of cotton vegetation and non-vegetation pixels were established based on the vegetation index threshold method which combines support vector machine classification and vegetation index.Finally,the accuracy difference in vegetation information extraction between the newly constructed and several published indices was compared.The results show that the accuracy of the information extracted by TRVI is higher than that of subdivision index of other visible light(FVC extraction precision in the first bud stage of cotton:R2=0.832,RMSE=2.307,nRMSE=4.405%;FVC extraction precision in the bud stage of cotton:R2=0.981,RMSE=1.393,nRMSE=1.984%;FVC extraction precision in the flowering stage of cotton:R2=0.893,RMSE=2.101,nRMSE=2.422%;FVC extraction precision in the boll stage of cotton:R2=0.958,RMSE=1.850,nRMSE=2.050%). 展开更多
关键词 COTTON UAV visible light images fractional vegetation cover vegetation index threshold method TRVI TBVI
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In vivo effects of neomycin sulfate on non-specific immunity,oxidative damage and replication of cyprinid herpesvirus 2 in crucian carp(Carassius auratus gibelio) 被引量:1
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作者 Penghui Sun Fei Yu +4 位作者 Jianfei lu Minli Zhang Hao Wang Dan Xu liqun lu 《Aquaculture and Fisheries》 2019年第2期67-73,共7页
Neomycin belongs to the family of 2-deoxystreptamine-containing aminoglycoside antibiotics.It is widely used for bacterial infections,targeting most gram-negative and some gram-positive bacteria.Neomycin has also been... Neomycin belongs to the family of 2-deoxystreptamine-containing aminoglycoside antibiotics.It is widely used for bacterial infections,targeting most gram-negative and some gram-positive bacteria.Neomycin has also been reported to show antiviral activity.Here,we evaluated the toxicity of neomycin sulfate,and investigated its effect on non-specific immunity and viral infection in crucian carp(Carassius auratus gibelio).The safe concentration of neomycin sulfate for crucian carp was determined to be 102.9 mg/kg in vivo.In oxidative damage assays,neomycin sulfate increased superoxide dismutase and catalase activity and decreased malondialdehyde in the liver of crucian carp.In non-specific blood immune assays,the white blood cell count and complement 3 content significantly increased after neomycin sulfate treatment,while no significant changes were observed in antibacterial or lysozyme activity.In a challenge test,neomycin sulfate protected the crucian carp from cyprinid herpesvirus 2(CyHV-2)infection and inhibited CyHV-2 replication.In cytotoxicity assays,low concentrations of neomycin sulfate had no cytotoxicity on cells from the fins of crucian carp.The results of the present study indicate that oral administration of neomycin sulfate reduced oxidative damage,enhanced immunity and provided protection against CyHV-2 in crucian carp. 展开更多
关键词 Neomycin sulfate Non-specific immunity Oxidative damage Crucian carp Cyprinid herpesvirus 2
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SUMOylation of Fragile X Mental Retardation Protein: A Critical Mechanism of FMRP-Mediated Neuronal Function
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作者 Mingzhu Tang liqun lu +1 位作者 Feng Xie Linxi Chen 《Neuroscience Bulletin》 SCIE CAS CSCD 2018年第6期1100-1102,共3页
Recently, Khayachi et al.;showed that fragile X mental retardation protein (FMRP) is an active substrate of the small ubiquitin-like modifier (SUMO) pathway in neurons.FMRP SUMOylation is induced by the activation... Recently, Khayachi et al.;showed that fragile X mental retardation protein (FMRP) is an active substrate of the small ubiquitin-like modifier (SUMO) pathway in neurons.FMRP SUMOylation is induced by the activation of metabotropic glutamate receptors (mGlu5Rs). FMRP 展开更多
关键词 FXS AD In SUMOylation of Fragile X Mental Retardation Protein
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Characterization of grass carp FosB, Fosl2, JunD transcription factors in response to GCRV infection
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作者 Fei Yu Wanjuan Li +2 位作者 Longlong Wang Shunzheng Que liqun lu 《Aquaculture and Fisheries》 2022年第3期304-312,共9页
Activating protein-1(AP-1)composed of Jun and Fos family proteins,which are frequently activated by pathogens to induce cellular inflammation and immune responses.In this study,by using RNA sequencing(RNA-seq)technolo... Activating protein-1(AP-1)composed of Jun and Fos family proteins,which are frequently activated by pathogens to induce cellular inflammation and immune responses.In this study,by using RNA sequencing(RNA-seq)technology,we found that FosB,Fosl2,and JunD expression was co-induced 12 and 20 h in grass carp(Ctenopharyngodon idella)kidney cell line(CIK cells)after grass carp reovirus(GCRV)infection.Full-length cDNA of the three genes were cloned and phylogenetic analysis indicated that the corresponding proteins shared high homology with those in higher vertebrates.The tissue distribution analysis of the three genes showed they were ubiquitously expressed in various tissues of healthy grass carp.In infection assay,FosB,Fosl2,and JunD were up-regulated and co-expressed 8 h after GCRV infection in CIK cells.Protein interaction and co-expression network analysis revealed that FosB,Fosl2,and JunD showed similar functions and were co-expressed in mammals and fish.Taken together,these results indicated that the co-expression of the AP-1 family proteins in grass carp uniformly responds to GCRV infection.These findings provided insights into the further study of AP-1 transcription factors function during grass carp reovirus infection. 展开更多
关键词 Grass carp reovirus FOSB Fosl2 JUND Co-expression Inflammation
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Combination of iron flocculation and qPCR for quantitative evaluation of virus-shedding intensity of goldfish Carassius auratus infected with cyprinid herpesvirus 2 in the water and the effect of sodium chlorite powder in blocking waterborne horizontal viral transmission
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作者 Yufei Zhai Fei Shen +1 位作者 Hao Wang liqun lu 《Aquaculture and Fisheries》 2024年第4期617-625,共9页
Cyprinid herpesvirus 2(CyHV-2),a member of the Alloherpesviridae family belonging to the genus Cyprinivirus,was initially isolated from goldfish(Carassius auratus)and has been recently emerging as a virulent pathogen ... Cyprinid herpesvirus 2(CyHV-2),a member of the Alloherpesviridae family belonging to the genus Cyprinivirus,was initially isolated from goldfish(Carassius auratus)and has been recently emerging as a virulent pathogen for cultured prussian carp(Carassius auratus gibelio)world-wide.In this study,a novel and effective method for concentration and quantification of live CyHV-2 virions from water was successfully established through coupling the iron flocculation with real time qPCR assay.Then,the shedding intensity of CyHV-2 in fish-tank water from artificially-challenged goldfish(25 fish/20 L)was monitored continuously for 7 days on a daily basis through quantitating viral genomic copy numbers by qPCR,and the maximum shedding level was determined to be 105 copies/L.Horizontal transmission research system was established by inoculating healthy goldfish in water spiked with serial dilution of CyHV-2 virions ranging from 107 to 103 copies/L.Our results indicated that water-borne CyHV-2 efficiently caused the infection of tested goldfish even in a concentration of 103 copies/L,and the overall transmission efficacy was not linearly correlated with the level of input virus in the fish tank.Commercial disinfectant Composite Sodium Chlorite Powder(CSCP)has been widely applied in aquaculture to control microbial infection through direct spill in the water,and its effect in inactivating the CyHV-2 infectivity remains unknown.We further determined that the EC50 of CSCP against 3.89 TCID50/mL CyHV-2 was close to 15.625μg/mL in vitro,and application of CSCP in a level as high as 60μg/mL(the safety concentration of CSCP for goldfish)couldn’t protect goldfish from CyHV-2 challenge through immersion.Thus,the disinfectant CSCP was regarded as none-effective for blocking CyHV-2 transmission in water during epidemic.Overall,our data provided quantitative data to demonstrate the shedding intensity of CyHV-2 in water,and CSCP was shown to be not effective in blocking water-borne horizontal transmission of CyHV-2 in goldfish.The virus-concentration protocol and virus-inhibition assay established here also paved the way for evaluating more commercial disinfectants in their effects in blocking water-borne horizontal transmission of CyHV-2. 展开更多
关键词 Cyprinid herpesvirus 2 Iron flocculation Virus shedding Water-borne horizontal transmission Composite sodium chlorite powder
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